Peroxiredoxin 6 expression is inversely correlated with nuclear factor-κB activation during Clonorchis sinensis infestation

Pak, J.H.,Son, W.C.,Seo, S.B.,Hong, S.J.,Sohn, W.M.,Na, B.K.,Kim, T.S. Pergamon ; Elsevier Science Ltd 2016 FREE RADICAL BIOLOGY AND MEDICINE Vol.99 No.-

Clonorchis sinensis is a carcinogenic human liver fluke. Its infection promotes persistent oxidative stress and chronic inflammation environments in the bile duct and surrounding liver tissues owing to direct contact with worms and their excretory-secretory products (ESPs), provoking epithelial hyperplasia, periductal fibrosis, and cholangiocarcinogenesis. We examined the reciprocal regulation of two ESP-induced redox-active proteins, NF-κB and peroxiredoxin 6 (Prdx6), during C. sinensis infection. Prdx6 overexpression suppressed intracellular free-radical generation by inhibiting NADPH oxidase2 and inducible nitric oxide synthase activation in the ESP-treated cholangiocarcinoma cells, substantially attenuating NF-κB-mediated inflammation. NF-κB overexpression decreased Prdx6 transcription levels by binding to two κB sites within the promoter. This transcriptional repression was compensated for by other ESP-induced redox-active transcription factors, including erythroid 2-related factor 2 (Nrf2), hypoxia inducible factor 1α (HIF1α), and CCAAT/enhancer-binding protein β (C/EBPβ). Distribution of immunoreactive Prdx6 and NF-κB was distinct in the early stages of infection in mouse livers but shared concomitant localization in the later stages. The intensity and extent of their immunoreactive staining in infected mouse livers are proportional to lesion severity and infection duration. The constitutive elevations of Prdx6 and NF-κB during C. sinensis infection may be associated with more severe persistent hepatobiliary abnormalities mediated by clonorchiasis.

Concurrent delivery of GM-CSF and B7-1 using an oncolytic adenovirus elicits potent antitumor effect

Choi, K-J,Kim, J-H,Lee, Y-S,Kim, J,Suh, B-S,Kim, H,Cho, S,Sohn, J-H,Kim, G E,Yun, C-O Nature Publishing Group 2006 Gene therapy Vol.13 No.13

Oncolytic adenoviral vectors are currently being developed as biologic anticancer agents. Coupling the lytic function of an oncolytic adenovirus (Ad) with its ability as a transgene delivery system represents a powerful extension of this methodology. A clear advantage is the amplification of a therapeutic gene, as replicating vectors would be able to infect and deliver the gene of interest to neighboring cells. Granulocyte–macrophage colony-stimulating factor (GM-CSF) is one of the most potent stimulators of a specific and long-lasting antitumor immunity and its important role in the maturation of antigen-presenting cells to induce T-cell activation has been well documented. Similarly, the B7 family has also been shown to play an integral role in mediating an antitumor response. Most tumor cells, however, lack the expression of these costimulatory molecules on their surface, thus escaping immune system recognition. To increase the antitumor effect of an oncolytic Ad, we have generated an E1B 55 kDa-deleted oncolytic adenoviral vector, YKL-GB, that expresses both GM-CSF and B7-1. The therapeutic efficacy of YKL-GB Ad was evaluated in immunocompetent mice bearing murine melanoma B16-F10 tumors. Significant inhibition of tumor growth was seen in mice treated with YKL-GB compared to those treated with the analogous vector, YKL-1. Moreover, YKL-GB oncolytic Ad demonstrated enhanced antitumor activity and higher incidences of tumor regression compared to a replication-incompetent Ad, dl-GB, which coexpresses GM-CSF and B7-1. Localized GM-CSF and B7-1 gene transfer also conferred long-lasting immunity against a tumor re-challenge. To establish that the observed antitumor effect is associated with the generation of a tumor-specific immune response, we carried out interferon-γ enzyme-linked immune spot assay. We observed that YKL-GB induced significantly higher immune cell activation than YKL-1. Furthermore, immunohistochemical studies demonstrated robust dendritic cells and CD4<SUP>+</SUP>/CD8<SUP>+</SUP> T-cell infiltration in these mice compared to the YKL-1-treated groups. In agreement with these results, splenocytes from tumor-bearing mice treated with YKL-GB expressed high levels of the costimulatory and activation molecules. These findings demonstrate the effectiveness of enhancing the immune response against tumors with an oncolytic Ad expressing both GM-CSF and B7-1 and provide a potential therapeutic strategy for the management of neoplasia.Gene Therapy (2006) 13, 1010–1020. doi:10.1038/sj.gt.3302759; published online 9 March 2006

Structural aspects of B*4617 molecule, a novel HLA-B*46 allele identified by sequence-based typing

Sohn, Y.-H.,Oh, H.-B.,Heo, Y.-S.,Kwon, O.-J. Blackwell Publishing Ltd 2009 Tissue antigens Vol.73 No.1

<P>New allele B*4617 showed one nucleotide difference with B*460101 at codon 167 (TGG→TCG).</P>

Effect of Crystallization Treatment on the Magnetic Properties of Amorphous Strips Based on Co-Fe-Ni-B-Si-Cr Containing Nitrogen

Cho H.J.,Kwon H.T.,Ryu H.H.,Sohn K.Y.,You B.S.,Park W.W. The Korean Powder Metallurgy Institute 2006 한국분말재료학회지 (KPMI) Vol.13 No.4

Co-Fe-Ni-B-Si-Cr based amorphous strips containing nitrogen were manufactured via melt spinning, and then devitrified by crystallization treatment at the various annealing temperatures of $300^{\circ}C{\sim}540^{\circ}C$ for up to 30 minutes in an inert gas $(N_2)$ atmosphere. The microstructures were examined by using XRD and TEM and the magnetic properties were measured by using VSM and B-H meter. Among the alloys, the amorphous ribbons of $Co_{72.6}Fe_{9.8}Ni_{5.5}B_{2.4}Si_{7.1}Cr_{2.6}$ containing 121 ppm of nitrogen showed relatively high saturation magnetization. The alloy ribbons crystallized at $540^{\circ}C$ showed that the grain size of $Co_{72.6}Fe_{9.8}Ni_{5.5}B_{2.4}Si_{7.1}Cr_{2.6}$ alloy containing 121 ppm of nitrogen was about f nm, which exhibited paramagnetic behavior. The formation of nano-grain structure was attributed to the finely dispersed Fe4N particles and the solid-solutionized nitrogen atoms in the matrix. Accordingly, it can be concluded that the nano-grain structure of 5nm in size could reduce the core loss within the normally applied magnetic field of 300A/m at 10kHz.

Gilbert damping in asymmetric MgO(Ta)/CoFeB/Ta(MgO) structures with perpendicular magnetic anisotropy

Sohn, J.W.,Song, H.S.,Kim, J.W.,Shin, I.J.,Min, B.C.,You, C.Y.,Shin, S.C. Elsevier 2016 Current Applied Physics Vol.16 No.4

<P>We investigate the ultrafast magnetization dynamics of perpendicularly magnetized MgO/CoFeB/Ta and Ta/CoFeB/MgO stack structures using an all-optical time-resolved magneto-optical Kerr measurement technique. We find that the effective Gilbert damping parameter alpha(eff) converges to a low value of similar to 0.02 in the high external field regime, and it stays unchanged at similar to 0.02 regardless of the pump fluence. Two asymmetric stack structures of CoFeB show identical dynamic behavior and Gilbert damping, implying that the influence of the under and top interfaces is not distinct. (C) 2015 Elsevier B.V. All rights reserved.</P>

ISFET 마이크로 센서용 전해질 분석기의 구현

崔埈赫,李秉柱,元哲昊,具成謨,金明南,金昶秀,孫炳基,曺辰鎬 경북대학교 센서기술연구소 1995 센서技術學術大會論文集 Vol.6 No.1

Electrolyte analyzer is one of widely used instrument in the hospitals and laboratories. In this paper, a desktop electrolyte analyzer which can detect and display both ion concentrations of hydrogen and sodium in the solution using ISFET is developed. We designed hardware of the system including signal-processing circuit for multiple sensor and flow system consist of a sample chamber unit and 12 actuators. For the efficient control of the system, 2-point calibration, measurement and washing algorithms are also developed. We compare the pH, pNa values measured by the developed system with those measured by reference system and verified the performance.

Pt deposited Pt-Pd/C electrocatalysts with the enhanced oxygen reduction activity

Park, J.H.,Sohn, Y.,Jung, D.H.,Kim, P.,Joo, J.B. Korean Society of Industrial and Engineering Chemi 2016 Journal of industrial and engineering chemistry Vol.36 No.-

<P>Reducing the cost of state-of-the-art Pt electrocatalysts while maintaining their oxygen reduction performance is always a hotspot in fuel cell research owing to their significant economic benefit for the commercialization of polymer electrolyte membrane fuel cells. Herein, we report a simple and cost-effective synthesis of Pt-Pd catalysts and a systematic study of their characteristics and catalytic performances for the oxygen reduction reaction (ORR). Pt-Pd bimetallic catalysts were prepared by a simple chemical deposition of Pt on the surface of Pd particles using a commercial Pd/C catalyst. During the synthesis, Pt precursor was reduced, and Pt layers were preferentially overgrown on the surface of the preexisting Pd particles resulting in Pd@Pt core-shell particles, which are favorable for ORR. By varying Pt precursors and the amount of Pt deposited, the physicochemical and electrochemical properties of the Pt-Pd catalysts were optimized. The formation of a thin Pt layer on Pd surface is more favorable, when using Pt(NH3)(4)Cl-2 center dot xH(2)O rather than H2PtCl6 center dot xH(2)O. As the amount of Pt increased from 0 to 10%, the surface properties of metal particles changed to similar to that of Pt, and the resulting catalysts mainly consist of a Pt-rich layer with a Pd core such as the Pd@Pt core-shell configuration. Pt(10%)Pd/C catalyst prepared by using Pt(NH3)(4)Cl-2 center dot xH(2)O exhibited a significant improvement in the ORR with the mass activities of 221 and 53 mA/mgpGm at 0.85 and 0.9 V, respectively, which are beatable values compared to those (219 and 59 mA/mg(Pt) at 0.85 and 0.9 V) of commercial Pt/C catalysts. The performance improvement of our bimetallic Pt-Pd/C catalysts mainly originate from the formation of an active Pt surface on the Pd core. In addition, considering that Pd is generally less expensive than Pt, these catalysts should have much better ORR performance and more feasibility of decreasing the total cost of fuel cells. In this study, the characteristics, electrochemical behaviors, and ORR performance improvements of the simply prepared Pt(x)-Pd/C core-shell catalysts were systemically investigated and are discussed. (C) 2016 The Korean Society of Industrial and Engineering Chemistry. Published by Elsevier B.V. All rights reserved.</P>

성인 지역 사회 폐렴의 원인으로서 아데노바이러스, RSV, 인플루엔자바이러스 및 파리인플루엔자바이러스의 역할에 관한 연구

김지희,박찬,이주연,안정배,박종원,신구철,손영모 국립보건연구원 1998 국립보건원보 Vol.35 No.-

Behavior of maltose-neopentyl glycol-3 (MNG-3) at the air/aqueous interface

Yoo, B.,Lee, J.,Choi, S.,Ryu, J.,Lee, H.,Chae, P.S.,Lee, S.U.,Maeda, M.,Sohn, D. Elsevier 2015 Colloids and surfaces. A, Physicochemical and engi Vol.484 No.-

<P>Integral membrane proteins (IMPS) performing a variety of cell functions comprise a significant fraction of the proteins encoded in an organism's genome. Maltose-neopentyl glycol-3 (MNG-3) was invented as a novel detergent to extract IMPs from the cell membranes; MNG-3 has an inherent flexibility originating from its central carbon and its arms comprising alkyl chains and maltose groups. Herein, we demonstrate the reverse-scissoring behavior of MNG-3 through the use of different subphase conditions at the air/water interface. The origin of its high performance was investigated by means of Langmuir techniques, X-ray reflectivity (XRR) analysis of its Langmuir-Schaefer (LS) film, Brewster angle microscope (BAM) and atomic force microscope (AFM) and computational simulation. (C) 2015 Elsevier B.V. All rights reserved.</P>

상부 소화관 출혈에 대한 내시경 직시하 Thrombin 산포 치험

박병권,전재범,손주현,이진,함준수,기춘석 최신의학사 1989 最新醫學 Vol.32 No.9