Adenosine triphosphate-based chemotherapy response assay-guided chemotherapy in unresectable colorectal liver metastasis

Hur, H,Kim, N K,Kim, H G,Min, B S,Lee, K Y,Shin, S J,Cheon, J H,Choi, S H Nature Publishing Group 2012 The British journal of cancer Vol.106 No.1

<P><B>Background:</B></P><P>This study aims to evaluate the effectiveness of adenosine triphosphate-based chemotherapy response assay (ATP-CRA)-guided neoadjuvant chemotherapy for increasing resectability in patients with unresectable colorectal liver metastasis.</P><P><B>Patients and methods:</B></P><P>Patients were randomised into two groups: Group A was treated by conventional chemotherapy regimen and Group B was treated by chemotherapy regimen according to the ATP-CRA. Three chemotherapeutic agents (5-fluorouracil, oxaliplatin and irinotecan) were tested by ATP-CRA and more sensitive agents were selected. Either FOLFOX or FOLFIRI was administered. Between Group A and B, treatment response and resectability were compared.</P><P><B>Results:</B></P><P>Between November 2008 and October 2010, a total 63 patients were randomised to Group A (<I>N</I>=32) or Group B (<I>N</I>=31). FOLFOX was more preferred in Group A than in Group B (26 out of 32 (81.3%) <I>vs</I> 20 out of 31 (64.5%)). Group B showed better treatment response than Group A (48.4% <I>vs</I> 21.9%, <I>P</I>=0.027). The resectability of hepatic lesion was higher in Group B (35.5% <I>vs</I> 12.5%, <I>P</I>=0.032). Mean duration from chemotherapy onset to the time of liver resection was 11 cycles (range 4–12) in Group A and 8 cycles (range 8–16) in Group B.</P><P><B>Conclusion:</B></P><P>This study showed that tailored-chemotherapy based on ATP-CRA could improve the treatment response and resectability in initially unresectable colorectal liver metastasis.</P>

쥐의 근육 및 간장 콜레스테롤 수준에 미치는 사료 중 n-3 / n-6 지방산 , PUFA / SFA 및 MUFA / SFA 비율의 반응표면 분석

박병성(B . S . Park),고병대(B . D . Ko),이영철(Y . C . Rhee),황보종(J . Howang - Bo),성기승(K . S . Seong),이남형(N . H . Lee) 한국영양사료학회 1995 韓國營養飼料學會誌 Vol.19 No.5

<i>CYP2A6</i> and <i>ERCC1</i> polymorphisms correlate with efficacy of S-1 plus cisplatin in metastatic gastric cancer patients

Park, S R,Kong, S-Y,Nam, B-H,Choi, I J,Kim, C G,Lee, J Y,Cho, S J,Kim, Y W,Ryu, K W,Lee, J H,Rhee, J,Park, Y-I,Kim, N K Nature Publishing Group 2011 The British journal of cancer Vol.104 No.7

<P><B>Background:</B></P><P>We evaluated the association between polymorphisms of cytochrome P450 2A6 (<I>CYP2A6</I>)/excision repair cross-complementation group 1 (<I>ERCC1</I>)/X-ray repair cross-complementing group 1(<I>XRCC1</I>) and treatment outcomes of metastatic gastric cancer (MGC) patients treated with S-1/cisplatin.</P><P><B>Methods:</B></P><P>Among MGC patients (<I>n</I>=108), who received S-1 (40 mg m<SUP>−2</SUP> b.i.d., days 1–14) and cisplatin (60 mg m<SUP>−2</SUP>, day 1) every 3 weeks, we analysed the wild-type allele (<I>W</I>) and variants (<I>V</I>) of <I>CYP2A6</I> (<I>*4</I>, <I>*7, *9, *10</I>), and the polymorphisms of <I>ERCC1</I> (rs11615, rs3212986) and <I>XRCC1</I> (rs25487).</P><P><B>Results:</B></P><P>Patients having fewer <I>CYP2A6</I> variants had better response rates (<I>W</I>/<I>W vs W</I>/<I>V</I> other than <I>*1/*4 vs V</I>/<I>V</I> or <I>*1/*4</I>=66.7 <I>vs</I> 58.3 <I>vs</I> 32.3% <I>P</I>=0.008), time to progression (TTP) (7.2 <I>vs</I> 6.1 <I>vs</I> 3.5 months, <I>P</I>=0.021), and overall survival (23.2 <I>vs</I> 15.4 <I>vs</I> 12.0 months, <I>P</I>=0.004). <I>ERCC1 19442C</I>><I>A</I> (rs3212986) was also associated with response rate (<I>C/C</I>, 46.7% <I>vs C/A</I>, 55.3% <I>vs A/A</I>, 87.5%) (<I>P</I>=0.048) and TTP (4.4 <I>vs</I> 7.6 <I>vs</I> 7.9 months) (<I>P</I>=0.012). Patients carrying both risk genotypes of <I>CYP2A6</I> (<I>V</I>/<I>V</I> or <I>1/*4</I>) and <I>ERCC1 19442C</I>><I>A</I> (<I>C/C</I>) <I>vs</I> those carrying none showed an adjusted odds ratio of 0.113 (<I>P</I>=0.004) for response, and adjusted hazard ratios of 3.748 (<I>P</I>=0.0001) for TTP and 2.961 (<I>P</I>=0.006) for death.</P><P><B>Conclusion:</B></P><P>Polymorphisms of <I>CYP2A6</I> and <I>ERCC1 19442C</I>><I>A</I> correlated with the efficacy of S-1/cisplatin.</P>

Multiple novel H5N6 highly pathogenic avian influenza viruses, South Korea, 2016

Lee, E.K.,Song, B.M.,Lee, Y.N.,Heo, G.B.,Bae, Y.C.,Joh, S.J.,Park, S.C.,Choi, K.S.,Lee, H.J.,Jang, I.,Kang, M.S.,Jeong, O.M.,Choi, B.K.,Lee, S.M.,Jeong, S.C.,Park, B.K.,Lee, H.S.,Lee, Y.J. Elsevier Science 2017 INFECTION GENETICS AND EVOLUTION Vol.51 No.-

<P>We report the identification of novel highly pathogenic avian influenza viruses of subtype H5N6, Glade 23.4.4, that presumably originated from China. In addition, reassortant strains with Eurasian lineage low pathogenic avian influenza viruses were isolated in wild birds and poultry in South Korea. The emergence of these novel H5N6 viruses and their circulation among bird populations are of great concern because of the potential for virus dissemination with intercontinental wild bird migration. (C) 2017 Elsevier B.V. All rights reserved.</P>

Electrical and luminescent properties and deep traps spectra of N-polar GaN films

Polyakov, A.Y.,Smirnov, N.B.,Govorkov, A.V.,Sun, Q.,Zhang, Y.,Cho, Y.S.,Lee, I.H.,Han, J. Elsevier 2010 Materials science and engineering B. Advanced Func Vol.166 No.1

Electrical and luminescent properties of N-polar undoped GaN films grown using low temperature GaN buffers on on-axis and miscut sapphire and on-axis AlN buffers are compared to the properties of Ga-polar films grown on low temperature GaN buffers. It is shown that the concentration of residual donors increases by about an order of magnitude for on-axis N-polar growth and by two orders of magnitude for off-axis growth compared to Ga-polar films. On-axis films for both Ga-polar and N-polar polarities show the presence of n<SUP>+</SUP> interfacial layers greatly influencing the apparent electron concentration and mobility deduced from capacitance-voltage C-V measurements. These interfacial layers are much less prominent in the miscut N-polar films. Growth on N-polar greatly increases the concentration of electron traps with activation energy of 0.9eV possibly related to Ga-interstitials.

Electrical properties of a Cu-germanide Schottky contact to n-type Ge depending on its microstructural evolution driven by rapid thermal annealing

Janardhanam, V.,Jyothi, I.,Lee, J.H.,Yun, H.J.,Won, J.,Lee, Y.B.,Lee, S.N.,Choi, C.J. Elsevier Sequoia 2017 THIN SOLID FILMS - Vol.632 No.-

The electrical properties of Cu-germanide(Cu<SUB>3</SUB>Ge)/n-type Ge Schottky contacts formed as a result of a solid state reaction between Cu and n-type Ge were investigated as a function of the rapid thermal annealing (RTA) temperature and correlated with its microstructural evolution driven by the RTA process. The variations of the barrier height of Cu<SUB>3</SUB>Ge/n-type Ge Schottky rectifiers caused by the RTA process were determined using current-voltage (I-V) and capacitance-voltage (C-V) methods. The Cu<SUB>3</SUB>Ge film formed after annealing at 400<SUP>o</SUP>C exhibited a relatively uniform surface and interface morphology. This led to the formation of a laterally homogenous Schottky barrier in the Cu<SUB>3</SUB>Ge/n-type Ge Schottky diode, resulting in an improvement of its rectifying I-V behavior. On the other hand, after annealing above 500<SUP>o</SUP>C, the Cu<SUB>3</SUB>Ge film was severely agglomerated without film continuity and eventually evolved into isolated islands at 600<SUP>o</SUP>C. Such structural degradation of Cu<SUB>3</SUB>Ge led to a rapid decrease in the barrier height and an increase in the reverse leakage current of the Cu<SUB>3</SUB>Ge/n-type Ge Schottky diode. The electric field dependence of the reverse current showed that the reverse leakage current in the Cu<SUB>3</SUB>Ge/n-type Ge Schottky diodes was dominated by a Poole-Frenkel emission mechanism, regardless of the RTA temperatures.

Anti-inflammatory activity of SMP30 modulates NF-κB through protein tyrosine kinase/phosphatase balance

Jung, K. J.,Lee, E. K.,Kim, S. J.,Song, C. W.,Maruyama, N.,Ishigami, A.,Kim, N. D.,Im, D. S.,Yu, B. P.,Chung, H. Y. Springer Science + Business Media 2015 Journal of molecular medicine Vol.93 No.3

<P>Recent studies on senescence marker protein-30 (SMP30) have shown that it has an important functional role in the aging process, but its precise participation in cellular works has not been fully determined. We hypothesize that SMP30 plays crucial roles in signaling processes by modulating the balance of protein tyrosine kinase (PTK)/protein tyrosine phosphatase (PTP) and in activating proinflammatory NF-kappa B. An experimental paradigm of gain and loss of SMP30 function was established using SMP30-overexpressed YPEN-1 cells (herein referred to as 'SMP30(+) cells') and SMP30 (Y/-) knockout mouse kidneys. The resulting data show that SMP30 expression suppressed oxidative stress-induced PTK/PTP dysregulation and PP1/2A inactivation in SMP30(+) cells, leading to the suppression of NF-kappa B activation. In the kidneys of SMP30 (Y/-) mice, SMP30 deficiency was found to induce NF-kappa B activation via the upstream signaling of NIK/IKK and MAPKs and to upregulate downstream NF-kappa B-responsive gene expression. In this study, we also demonstrate for the first time that SMP30 deficiency induced PTK activity in SMP30 (Y/-) kidneys, thereby significantly increasing the tyrosine phosphorylation of a catalytic subunit of PP2A (PP2Ac-Tyr307). Based on these findings, we propose that SMP30 involves NF-kappa B regulation through the PTK/PTP balance and that the age-related decrease of SMP30 causes NF-kappa B activation, which contributes to an exacerbation of the inflammatory process during aging. Key message SMP30-deficient mice induced a shorter lifespan and redox changes. Overexpression of SMP30 prevented oxidative stress insults. The depletion of SMP30 increased redox-related PTK/PTP imbalance and PP1/PP2A inactivation. The depletion of SMP30 caused an elevation of NF-kappa B-responsive inflammatory markers. SMP30 may be a potent inhibitory protein against oxidative stress and chronic inflammation.</P>

중년남성의 건강증진행위에 영향을 미치는 요인에 관한 연구

김수지,김하정,박누리,박지성,이지영,이혜미,최한나,한유빈,홍유나,이건정,이혜림 이화여자대학교 간호과학대학 2016 이화간호학회지 Vol.- No.50

Purpose: The purpose of this study was to figure out general characteristics, health perception, self-efficacy, and family support influencing on health promotion behavior of middle-aged male. Method: In this descriptive correlation design study, 320 middle-aged male were recruited through convenient sampling between October and December, 2015. Questionnaires used for this study were HPLP(Health Promotion Lifestyle Profile), HPS(Health perception Scale), Self-Efficacy Scale developed by Hong, and Family Support Scale developed by Park. Data were statistically analyzed using SPSS 22.0 program with descriptive statistics, independent t-test, one way ANOVA with Scheffe's test, and multiple linear regression. Result: It had significant influence to correlation among health promotion behavior, health perception, self-efficacy, and family support of middle-aged male. Also, the most influenced factor to health promotion behavior showed health perception. Conclusion: The result of this study suggested that health perception significantly influenced to health promotion behavior. Therefore, health perception educating program for health promotion behavior of middle-aged male. And further studies are required with more sample of various group and systematic data collecting methods to confirm these results.

Prevalence of Hepatitis C antibody in Patients with chronic liver disease and hepatocellular carcinoma in Korean

Park, B.C.,Han, B.H.,Ahn, S.Y.,Lee, S.W.,Lee, D.H.,Lee, Y.N.,Seo, J.H.,Kim, K.W. 부산대학교 유전공학연구소 1995 분자생물학 연구보 Vol.11 No.-

To investigate the contribution of hepatitis C virus (HCV) to chronic liver disease and hepatocellular carcinoma (HCC) in Korea. antibodies to HCV (anti-HCV) were tested by enzyme immunoassay in 1759 patients with chronic liver disease and HCC, and in 808 healthy adults. The prevalence of anti-HCV was 1.6% in 808 controls. Anti-HCV was present in 32 (7.7%) of 418 hepatitis B surface antigen (HBsAg)-positive and 128 (53.1%) of 241 HBsAg-negative patients with chronic hepatitis, 16 (6.0%) of 265 HBsAg-positive and 90 (30.5%) of 295 HBsAg-negative patients with liver cirrhosis, and 16 (4.8%) of 330 HBsAg-positive and 61 (29.0%) of 210 HBsAg-negative patients with HCC. Antibodies to hepatitis B core antigen (anti-HBc) were present in 80-88% of patients who were seropositive for anti-HCV and seronegative for HBsAg. Among the sera from 114 patients with HBsAg-negative and anti-HCV-positive chronic liver diseases, HBV DNA and HCV RNA were detected by polymerase chain reaction (POR) in 54 (47.4%) and 61 (53.3%), respectively. Both HBV DNA and HCV RNA were detected in 4 (4.4%) samples. The mean age of the patients with both HBsAg and anti-HCV was not different from that of patients who were seropositive for HBsAg alone. These findings indicate that current and/or past HBV infection is still the main cause of chronic liver disease in Korea. Although multivariate analysis showed that anti-HCV is a risk factor for chronic hepatitis, cirrhosis of the liver and HCC, PCR data for HBV DNA and HCV RNA indicate that HCV infection plays only a minor role in HBsAg-positive as well as in HBsAg-negative liver disease and does not accelerate the development of HCC in HBV carriers.

Isolation and characterization of a novel analyte from Bacillus subtilis SC-8 antagonistic to Bacillus cereus

Lee, N.K.,Yeo, I.C.,Park, J.W.,Kang, B.S.,Hahm, Y.T. Society for Bioscience and Bioengineering, Japan ; 2010 Journal of bioscience and bioengineering Vol.110 No.3

In this study, an effective substance was isolated from Bacillus subtilis SC-8, which was obtained from traditionally fermented soybean paste, cheonggukjang. The substance was purified by HPLC, and its properties were analyzed. It had an adequate antagonistic effect on Bacilluscereus, and its spectrum of activity was narrow. When tested on several gram-negative and gram-positive foodborne pathogenic bacteria such as Salmonella enterica, Salmonella enteritidis, Staphylococcus aureus, and Listeria monocytogenes, no antagonistic effect was observed. Applying the derivative from B. subtilis SC-8 within the same genus did not inhibit the growth of major soybean-fermenting bacteria such as Bacillus subtilis, Bacillus licheniformis, and Bacillus amyloquefaciens. The range of pH stability of the purified antagonistic substance was wide (from 4.0 to >10.0), and the substance was thermally stable up to 60<SUP>o</SUP>C. In the various enzyme treatments, the antagonistic activity of the purified substance was reduced with proteinase K, protease, and lipase; its activity was partially destroyed with esterase. Spores of B. cereus did not grow at all in the presence of 5μg/mL of the purified antagonistic substance. The isolated antagonistic substance was thought to be an antibiotic-like lipopeptidal compound and was tentatively named BSAP-254 because it absorbed to UV radiation at 254nm.