http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
The CCR4-NOT Complex Is Implicated in the Viability of Aneuploid Yeasts
Tange, Yoshie,Kurabayashi, Atsushi,Goto, Bunshiro,Hoe, Kwang-Lae,Kim, Dong-Uk,Park, Han-Oh,Hayles, Jacqueline,Chikashige, Yuji,Tsutumi, Chihiro,Hiraoka, Yasushi,Yamao, Fumiaki,Nurse, Paul,Niwa, Osami Public Library of Science 2012 PLoS genetics Vol.8 No.6
<P>To identify the genes required to sustain aneuploid viability, we screened a deletion library of non-essential genes in the fission yeast <I>Schizosaccharomyces pombe</I>, in which most types of aneuploidy are eventually lethal to the cell. Aneuploids remain viable for a period of time and can form colonies by reducing the extent of the aneuploidy. We hypothesized that a reduction in colony formation efficiency could be used to screen for gene deletions that compromise aneuploid viability. Deletion mutants were used to measure the effects on the viability of spores derived from triploid meiosis and from a chromosome instability mutant. We found that the CCR4-NOT complex, an evolutionarily conserved general regulator of mRNA turnover, and other related factors, including poly(A)-specific nuclease for mRNA decay, are involved in aneuploid viability. Defective mutations in CCR4-NOT complex components in the distantly related yeast <I>Saccharomyces cerevisiae</I> also affected the viability of spores produced from triploid cells, suggesting that this complex has a conserved role in aneuploids. In addition, our findings suggest that the genes required for homologous recombination repair are important for aneuploid viability.</P><P><B>Author Summary</B></P> <P>Aneuploidy is a major cause of abortive development and is implicated in tumorigenesis in humans. Recent studies revealed that the increased need for protein degradation might account for the detrimental effects of aneuploidy on a cell. Here, we investigated the genetic systems responsible for aneuploid viability. Using a collection of gene deletions in fission yeast, we isolated mutants that affect aneuploid viability. We found that an evolutionarily conserved transcription regulator, the CCR4-NOT complex, and its related factors are required for aneuploid viability, suggesting that regulation of mRNA turnover is required to tolerate aneuploidy. In addition, homologous recombination repair is important for aneuploid viability.</P>
Kazuya Kariyama,Kazuhiro Nouso,Atsushi Hiraoka,Hidenori Toyoda,Toshifumi Tada,Kunihiko Tsuji,Toru Ishikawa,Takeshi Hatanaka,Ei Itobayashi,Koichi Takaguchi,Akemi Tsutsui,Atsushi Naganuma,Satoshi Yasuda 대한간암학회 2024 대한간암학회지 Vol.24 No.1
Background/Aim: The aim of this study was to compare the therapeutic efficacy of ablation and surgery in solitary hepatocellular carcinoma (HCC) measuring ≤5 cm with a large HCC cohort database. Methods: The study included consecutive 2,067 patients with solitary HCC who were treated with either ablation (n=1,248) or surgery (n=819). Th e patients were divided into three groups based on the tumor size and compared the outcomes of the two therapies using propensity score matching. Results: No significant difference in recurrence-free survival (RFS) or overall survival (OS) was found between surgery and ablation groups for tumors measuring ≤2 cm or >2 cm but ≤3 cm. For tumors measuring >3 cm but ≤5 cm, RFS was significantly better with surgery than with ablation (3.6 and 2.0 years, respectively, P=0.0297). However, no significant difference in OS was found between surgery and ablation in this group (6.7 and 6.0 years, respectively, P=0.668). Conclusion: The study suggests that surgery and ablation can be equally used as a treatment for solitary HCC no more than 3 cm in diameter. For HCCs measuring 3-5 cm, the OS was not different between therapies; thus, ablation and less invasive therapy can be considered a treatment option; however, special caution should be taken to prevent recurrence.