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Ultrasonic Manipulation of Magnetic Particles in a Microfluidic Channel
Siddique, Ariful Haque,Cho, Seung Hyun,Ahn, Bongyoung,Kim, CheolGi Korean Society for Precision Engineering 2014 International Journal of Precision Engineering and Vol.15 No.7
In microfludic biosensor applications, many kinds of magnetic particles have been employed due to their bioaffinity property, so the appropriate manipulation of magnetic microparticles is important. In this work, we demonstrated the alignment of magnetic beads (diameter <TEX>$2.8{\mu}m$</TEX>) and nanowires (length <TEX>${\leq}6{\mu}m$</TEX> & diameter <TEX>${\approx}$</TEX> 50 nm) using ultrasonic standing wave (USW) in a steel-acrylic microfluidic channel (<TEX>$660{\mu}m$</TEX> width) in the aim of concentrating them to a sensor area to enhance the performance of a sensor. Ultrasonic standing wave field generates the radiation force to concentrate microparticles at the pressure nodal planes. To generate a standing wave inside the channel, an ultrasound of 2.25 MHz resonance frequency was applied as a resonance frequency of the channel. Moreover, it was demonstrated here that the position of the aligned magnetic particles could be controlled by changing the applied frequency. These successful alignment and position control can be a milestone for future research which targets to improve the sensitivity of microfluidic sensors using magnetic particles.
Use of Frequency Swept Ultrasound to Manipulate Nb2-11 Live Cells in a Microfluidic Channel
A. K. M. Ariful Haque Siddique,김철기,조승현,안봉영,윤동진 한국물리학회 2014 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.64 No.2
Recently microfluidic manipulation of live cell has been attracting attention because it is necessaryfor fast and accurate bioassays of cells within miniaturized devices like a lab-on-a-chip. This workconcerns the alignment, translocation and concentration of live cells in a microfluidic channel byutilizing an ultrasonic standing wave. Among many ultrasonic manipulation methods, the techniqueusing frequency swept ultrasound which was proposed in a previous study offers excellent particlemanipulation performance, but that has been demonstrated only for biocompatible polystyrenemicrospheres with several different diameters, not actual biological cells. However, the techniqueneeds to be verified for real cells because sizes and properties are little different with each other. Thus, in this work, the feasibility of using the ultrasonic frequency sweep method to manipulate liveNb2-11 cells was investigated experimentally The cells were aligned in a plane, moved to one sideof a channel, and finally filtered out by sending them to one outlet with ultrasound. The frequencyregion of the applied ultrasound was between 1.75 MHz and 3.05 MHz. From experimental results,the cell behavior showing perfect alignment, almost perfect translocation and hence considerableaggregation proved the feasibility of using the frequency sweep method though the force acting onthe Nb2-11 cells are much smaller than the force acting on polystyrene beads due to the differencesin their sizes and mechanical properties.
초음파를 이용한 미세채널 내 유동중 이종 미세입자 분리
조승현,A. K. M. Ariful Haque Siddique,서대철 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.4
Ultrasound can be used to manipulate microparticles since the acoustic radiation force of the ultrasound can exert force on the particles. This work concerns a particle separation technique using ultrasound. We propose a novel method to separate mixed two kinds of particles by adjusting the frequency sweep time of the exciting ultrasound. A modulized rrucrochannel having V-shaped branch was manufactured and ultrasound was excited from one side of the channel. The repeating frequency sweep can move the flowing particles to the side of the channel wall . Particles respond only over specific sweep time since they cannot keep pace with the rapidly changing ultrasonic field. So, we can send two kinds of particles into each outlet using their different minimum sweep time. Experiments confirm the successful performance of the proposed method.
초음파 주파수 스윕 시간조정을 통한 혼합 미세입자 분리 기법
조승현(Seung Hyun Cho),A. K. M. Ariful Haque Siddique 대한기계학회 2010 대한기계학회 춘추학술대회 Vol.2010 No.3
Ultrasound can be used to manipulate microparticles since the acoustic radiation force of the ultrasound can exert force on the particles. This work concerns a particle separation technique using ultrasound. We propose a novel method to separate mixed two kinds of particles by adjusting the frequency sweep time of the exciting ultrasound. A modulized microchannel having V-shaped branch was manufactured and ultrasound was excited from one side of the channel. The repeating frequency sweep can move the flowing particles to the side of the channel wall. Particles respond only over specific sweep time since they cannot keep pace with the rapidly changing ultrasonic field. So, we can send two kinds of particles into each outlet using their different minimum sweep time. Experiments confirm the successful performance of the proposed method.
Dae-Cheol Seo,A. K. M. Ariful Haque Siddique,안봉영,Cheol Gi Kim,Seung Hyun Cho 대한기계학회 2013 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.27 No.3
Many studies have been conducted on the filtration of microparticles using the acoustic radiation force of ultrasonic standing wave. The present work concerns a flow-through particle filtration method by utilizing frequency varying ultrasound. The periodical frequency sweep of the ultrasonic standing wave translocates particles across a microchannel, where particles in fluid flow are filtrated without barriers. The present filtration technique in a microfluidic channel was proposed conceptually in the 199 0s. However, its experimental realization on actual particles in a microfluidic channel has not been carried out in a notable way. Several sizes of polystyrene microspheres (10 µm to 90 µm) and silicon carbide (SiC) particles (37 µm) suspended in water were applied as a test sample. For filtration of those particles, a Y-branched microfluidic channel with one inlet and two outlets was made out of steel and acrylic as a form of modulized device. Ultrasound of a few MHz in band frequency (1.75 MHz to 3.05 MHz) was transmitted into one side of the channel wall to generate a standing wave field in fluid flow. The periodical frequency sweep operation showed successful filtration performance, whereby particles in water flowed into one outlet and purified water flowed into the other outlet of the Y branch of the channel.
ISLAM, K.M. Anwarul,SADEKIN, Mohammad Shamsus,RAHMAN, Md. Tahidur,CHOWDHURY, Md. Ariful Haque Korea Distribution Science Association 2021 The Journal of Asian Finance, Economics and Busine Vol.8 No.3
Although corporate social responsibility (CSR) is an extensively studied topic, its determinants in the field of Islamic banking are scarce. In Bangladesh, CSR plays a vital role in gaining customer loyalty and confidence. Therefore, this research aims to identify and analyze the influence of the Shariah Supervisory Board (SSB) and the Shariah Audit Committee (SAC) on CSR adoption in Islamic banks in Bangladesh. The study population is managers and second managers of 160 Islamic bank branches of different commercial banks in Dhaka, Bangladesh. The sampling technique used is convenience sampling where the first available primary data source was used for the research without additional requirements. The study developed a survey questionnaire from examining previous related studies in Islamic banking and CSR context. The final sample size in this research was n = 309, indicating the survey response rate was about 97%. The study used SPSS 23.0 software to interpret the statistical findings, and the findings revealed that support from the SSB and the presence of a strong and effective SAC has a strong correlation with CSR adoption and significantly influence CSR adoption in Islamic banks in Bangladesh. Finally, the study proposes several significant and crucial policy guidelines for Islamic bank branches to adopt CSR activities.
Hasan, Md. Mahmudul,Islam, Md. Shihabul,Hoque, Kazi Md. Faisal,Haque, Ariful,Reza, Md Abu Korean Society of ToxicologyKorea Environmental Mu 2019 Toxicological Research Vol.35 No.3
Citrus macroptera (Rutaceae) has long been used in folk medicine in Bangladesh. Considering the folkloric context, this study was aimed to scrutinize anti-proliferative activity of C. macroptera fruit pulp juice (CMFPJ) against Ehrlich's ascites carcinoma (EAC). The anti-proliferative capacity of CMFPJ was investigated and confirmed primarily using MTT assay. In vivo anti-proliferative aptitude of CMFPJ was investigated with 25, 50, and 100 mg/kg/day intraperitoneal (i.p.) treatment. Anti-proliferative efficacy of CMFPJ was assessed based on EAC growth inhibition. CMFPJ inhibited EAC growth in vitro in a dose-dependent manner. And the percentages of in vivo EAC growth inhibition were 19.53, 49.2, and 68.9% at 25, 50, and 100 mg/kg CMFPJ respectively. CMFPJ significantly induced expression of apoptosis regulatory genes caspase-8, caspase-9, cytochrome-c, and caspase-3. This considerable anti-cancer activity was perhaps due to combinatorial effect of lectin, polyphenols, and flavonoids present in CMFPJ.
Md. Mahmudul Hasan,Md. Shihabul Islam,Kazi Md. Faisal Hoque,Ariful Haque,Md Abu Reza 한국독성학회 2019 Toxicological Research Vol.35 No.3
Citrus macroptera (Rutaceae) has long been used in folk medicine in Bangladesh. Considering the folkloric context, this study was aimed to scrutinize anti-proliferative activity of C. macroptera fruit pulp juice (CMFPJ) against Ehrlich’s ascites carcinoma (EAC). The anti-proliferative capacity of CMFPJ was investigated and confirmed primarily using MTT assay. In vivo anti-proliferative aptitude of CMFPJ was investigated with 25, 50, and 100 mg/kg/day intraperitoneal (i.p.) treatment. Anti-proliferative efficacy of CMFPJ was assessed based on EAC growth inhibition. CMFPJ inhibited EAC growth in vitro in a dose-dependent manner. And the percentages of in vivo EAC growth inhibition were 19.53, 49.2, and 68.9% at 25, 50, and 100 mg/kg CMFPJ respectively. CMFPJ significantly induced expression of apoptosis regulatory genes caspase-8, caspase-9, cytochrome-c, and caspase-3. This considerable anti-cancer activity was perhaps due to combinatorial effect of lectin, polyphenols, and flavonoids present in CMFPJ.