http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : Ardelli, Bernadette F. (검색결과 1 건)
- 무료
- 기관 내 무료
- 유료
-
Ahn, Seong Kyu,Cho, Pyo Yun,Na, Byoung-Kuk,Hong, Sung-Jong,Nam, Ho-Woo,Sohn, Woon-Mok,Ardelli, Bernadette F.,Park, Yun-Kyu,Kim, Tong-Soo,Cha, Seok Ho Springer-Verlag 2016 Parasitology research Vol.115 No.1
<P>A complementary DNA (cDNA) encoding a glucose transporter of Clonorchis sinensis (CsGLUT) was isolated from the adult C. sinensis cDNA library. The open reading frame of CsGLUT cDNA consists of 1653 base pairs that encode a 550-amino acid residue protein. Hydropathy analysis suggested that CsGLUT possess 12 putative membrane-spanning domains. The Northern blot analysis result using poly(A)(+)RNA showed a strong band at similar to 2.1 kb for CsGLUT. When expressed in Xenopus oocytes, CsGLUT mediated the transport of radiolabeled deoxy-D-glucose in a time-dependent but sodium-independent manner. Concentration-dependency results showed saturable kinetics and followed the Michaelis-Menten equation. Nonlinear regression analyses yielded a Km value of 588.5 +/- 53.0 mu M and a Vmax value of 1500.0 +/- 67.5 pmol/oocyte/30 min for [1,2-H-3]2-deoxy-D-glucose. No trans-uptakes of bile acid (taurocholic acid), amino acids (tryptophan and arginine), or p-aminohippuric acid were observed. CsGLUT-mediated transport of deoxyglucose was significantly and concentration-dependently inhibited by radio-unlabeled deoxyglucose and D-glucose. 3-O-Methylglucose at 10 and 100 mu M inhibited deoxyglucose uptake by similar to 50 % without concentration dependence. No inhibitory effects by galactose, mannose, and fructose were observed. This work may contribute to the molecular biological study of carbohydrate metabolism and new drug development of C. sinensis.</P>
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
