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RISS 인기검색어
- 검색키워드
- 저자 : Antje Baeumner (검색결과 3 건)
- 무료
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The Micro-Total Analytical System for the Detection of Bacteria/Viruses
Min, Junhong,Baeumner, Antje 한국공업화학회 2003 Journal of Industrial and Engineering Chemistry Vol.9 No.1
Gene-based biosensors have allowed fast and specific detection of pathogenic bacteria and viruses in past decade. In addition to nucleic acid biosensors, DNA arrays or gene have been researched to detect sequence-specific information in a faster, simpler and cheaper manner or the micro-total analytical system (μ-TAS), also known as "Lab-on-a-Chip", not only incorporates detection but additionally sample preparation and a digital readout all in one device. A variety of fields have to be combined to realize these micro-total analysis systems. Thus, several biological, chemical and engineering techniques are introduced in this paper to develop μ-TAS for the detection of viruses and bacteria. This short review article on the fabrication of μ-TAS for the detection of bacteria and viruses intended to provide an overview of the design criteria and potential fabrication techniques. Obviously, there are several different ways of realizing a successful μTAS, and only a limited number of examples were presented in this paper. Detection mechanisms such as microarrays, which can detect DNA/RNA molecules or proteins, are already commercialized by several companies (Nanogenu®, BioMicro systems™). They typically use silicon, or glass with standard photolithography techniquies and can even be connected to PDMS with molding and soft lithography to create channels and reactors. However, these arrays only represent the detection part of an entire μTAS, Fabricating μ TAS devices is technologically no challenge anymore, especially since seldomly submicrometer dimensions are required. However, the design of a functional μ-TAS and the integration of the diffcrent modules into one system are main issues to be consdered in the future. Given the impressive progress in the field of μ-TAS, there is no doubt that it will be the major sensing device for clinical diagnostic as well as for the detection of bacteria and viruses in the environment and for food safety.
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A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples
Vijayarani Kumanan,Sam R. Nugen,Antje J. Baeumner,Yung-Fu Chang 대한수의학회 2009 JOURNAL OF VETERINARY SCIENCE Vol.10 No.1
A simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small number (ten) of viable Mycobacterium (M.) avium subsp. paratuberculosis (MAP) cells in fecal samples. The assays are based on the identification of the RNA of the IS900 element of MAP. For the assay, RNA was extracted from fecal samples spiked with a known quantity of (101 to 106) MAP cells and amplified using RT-PCR and identified by the LF biosensor and the microtiter plate assay. While the LF biosensor assay requires only 30 min of assay time, the overall process took 10 h for the detection of 10 viable cells. The assays are based on an oligonucleotide sandwich hybridization assay format and use either a membrane flow through system with an immobilized DNA probe that hybridizes with the target sequence or a microtiter plate well. Signal amplification is provided when the target sequence hybridizes to a second DNA probe that has been coupled to liposomes encapsulating the dye, sulforhodamine B. The dye in the liposomes provides a signal that can be read visually, quantified with a hand-held reflectometer, or with a fluorescence reader. Specificity analysis of the assays revealed no cross reactivity with other mycobacteria, such as M. avium complex, M. ulcerans, M. marium, M. kansasii, M. abscessus, M. asiaticum, M. phlei, M. fortuitum, M. scrofulaceum, M. intracellulare, M. smegmatis, and M. bovis. The overall assay for the detection of live MAP organisms is comparatively less expensive and quick, especially in comparison to standard MAP detection using a culture method requiring 6-8 weeks of incubation time, and is significantly less expensive than real-time PCR.
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