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Ali Mehrban Jafarlou,Saeed Piri Pirivatlo,Behrouz Salehi,Amir Hoshang Hoseynzadeh Mogbli 한국작물학회 2021 Journal of crop science and biotechnology Vol.24 No.4
This survey is aimed at the assessment of the possible efect of diferent plant growth regulators (PGRs) on the micropropagation of commercial sweet cherry rootstocks Gisela 12 (Prunus cerasus×Prunus canescens), and Maxma 14 (Prunus avium×Prunus mahaleb). To study Gisela 12 and Maxma 14 micropropagation were assessed utilizing lateral buds. The treatments used for the establishment and shoots proliferation included MS medium fortifed by benzyl amino purine (BAP) (0.0, 0.5, 1.0, 1.5 and 2.0 mg L−1) and indol-3-butyric acid (IBA) (0, 0.25, 0.50 and 1.0 mg L−1). For rooting, IBA was used at four levels (0.0, 0.25, 0.50, and 1.0 mg L−1) in combination with 0.0, 0.25, and 0.50 mg L−1 naphthaleneacetic acid (NAA) in full and half-strength MS media. Maximum shoot multiplication for Gisela 12 (6.2 shoots/explants) and Maxma 14 (4.4 shoots/explants) were observed on MS medium reinforced with 2.0 mg L−1 BAP with 0.25 mg L−1 IBA. The best rooting response of Gisela 12 (100%) and Maxma 14 (88.88%) was also achieved on half-strength MS including 1.0 mg L−1 IBA plus 0.25 NAA and 0.5 IBA plus 0.25 NAA, respectively. The ISSR banding profle showed the genetic stability of propagated plants along with the mother plant. This is the frst study evaluating the use of molecular markers for genetic uniformity test, which can be applied for large-scale propagation.