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RISS 인기검색어
- 검색키워드
- 저자 : Ali Akbar Shafiee (검색결과 3 건)
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UBE2Q1 in a Human Breast Carcinoma Cell Line: Overexpression and Interaction with p53
Shafiee, Sayed Mohammad,Rasti, Mozhgan,Seghatoleslam, Atefeh,Azimi, Tayebeh,Owji, Ali Akbar Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.9
The p53 tumor suppressor protein is a principal mediator of growth arrest, senescence, and apoptosis in response to a broad array of cellular damage. p53 is a substrate for the ubiquitin-proteasome system, however, the ubiquitin-conjugating enzymes (E2s) involved in p53 ubiquitination have not been well studied. UBE2Q1 is a novel E2 ubiquitin conjugating enzyme gene. Here, we investigated the effect of UBE2Q1 overexpression on the level of p53 in the MDA-MB-468 breast cancer cell line as well as the interaction between UBE2Q1 and p53. By using a lipofection method, the p53 mutated breast cancer cell line, MDA-MB-468, was transfected with the vector pCMV6-AN-GFP, containing UBE2Q1 ORF. Western blot analysis was employed to verify the overexpression of UBE2Q1 in MDA-MB-468 cells and to evaluate the expression level of p53 before and after cell transfection. Immunoprecipitation and GST pull-down protocols were used to investigate the binding of UBE2Q1 to p53. We established MDA-MB-468 cells that transiently expressed a GFP fusion proteins containing UBE2Q1 (GFP-UBE2Q1). Western blot analysis revealed that levels of p53 were markedly lower in UBE2Q1 transfected MDA-MB-468 cells as compared with control MDA-MB-468 cells. Both in vivo and in vitro data showed that UBE2Q1 co-precipitated with p53 protein. Our data for the first time showed that overexpression of UBE2Q1can lead to the repression of p53 in MDA-MB-468 cells. This repression of p53 may be due to its UBE2Q1 mediated ubiquitination and subsequent proteasome degradation, a process that may involve direct interaction of UBE2Q1with p53.
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Gas transport behavior of DMDCS modified MCM_48/polysulfone mixed matrix membrane coated by PDMS
Abolfazl Jomekian,Seyed Ali Akbar Mansoori,Negin Monirimanesh,Alireza Shafiee 한국화학공학회 2011 Korean Journal of Chemical Engineering Vol.28 No.10
Mesoporous MCM-48 silica was synthesized by templating method and the structure of particles was characterized by XRD, TEM and N_2 adsorption techniques. The surface modification of particles in order for introducing into PSF matrix was performed by dimethyldichlorosilane (DMDCS) silylation agent. SEM images of as-synthesized and modified MCM-48/PSF MMMs indicate that in the modified MCM-48 silica particles adhered well to the PSF matrix and that the synthesized MMMs were defect free. The incorporation of MCM-48 particles in to the PSF matrix and also surface coating of these MMMs by polydimethylsiloxane (PDMS) were performed. The quality of surface coating was investigated by SEM images and permeability tests. For all gases tested (N_2, CO_2, CH4 and O_2), the permeabilities increased in proportion to the weight percent of MCM-48 present in the film and the calculated CO_2/CH_4 and O_2/N_2 selectivities of PDMS coated membranes showed enhancement in ideal and actual selectivities both.
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Fluid-structure coupling of concentric double FGM shells with different lengths
Ehsan Moshkelgosha,Ehsan Askari,정경훈,Ali Akbar Shafiee 국제구조공학회 2017 Structural Engineering and Mechanics, An Int'l Jou Vol.61 No.2
The aim of this study is to develop a semi-analytical method to investigate fluid-structure coupling of concentric double shells with different lengths and elastic behaviours. Co-axial shells constitute a cylindrical circular container and a baffle submerged inside the stored fluid. The container shell is made of functionally graded materials with mechanical properties changing through its thickness continuously. The baffle made of steel is fixed along its top edge and submerged inside fluid such that its lower edge freely moves. The developed approach is verified using a commercial finite element computer code. Although the model is presented for a specific case in the present work, it can be generalized to investigate coupling of shellplate structures via fluid. It is shown that the coupling between concentric shells occurs only when they vibrate in a same circumferential mode number, n. It is also revealed that the normalized vibration amplitude of the inner shell is about the same as that of the outer shell, for narrower radial gaps. Moreover, the natural frequencies of the fluid-coupled system gradually decrease and converge to the certain values as the gradient index increases.
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