Development of a Perfusable Vessel Incorporated in vitro Platform for Co-culturing with Engineered Skeletal Muscle for Inducing Angiogenesis

Mst Zobaida Akter(M. Z. Akter),Ashfaq Ahmad(A. Ahmad),S.-Y. Kim(김서연),C. Lee(이창호),Y.-J. Choi(최영진),H.-G. Yi(이희경) Korean Society for Precision Engineering 2024 한국정밀공학회 학술발표대회 논문집 Vol.2024 No.5

Zooplankton community of Ross Sea, Antarctic Ocean in Austral summer

Akter Masuma,Won-Gyu Park,Hyoung-Sul La 한국수산과학회 양식분과 2020 한국수산과학회 양식분과 학술대회 Vol.2020 No.7

Ultrasensitive Nanoimmunosensor by coupling non-covalent functionalized graphene oxide platform and numerous ferritin labels on carbon nanotubes

Akter, Rashida,Jeong, Bongjin,Choi, Jong-Soon,Rahman, Md. Aminur Elsevier Applied Science 2016 Biosensors & bioelectronics Vol. No.

<P><B>Abstract</B></P> <P>An ultrasensitive electrochemical nanostructured immunosensor for a breast cancer biomarker carbohydrate antigen 15-3 (CA 15-3) was fabricated using non-covalent functionalized graphene oxides (GO/Py-COOH) as sensor probe and multiwalled carbon nanotube (MWCNTs)-supported numerous ferritin as labels. The immunosensor was constructed by immobilizing a monoclonal anti-CA 15-3 antibody on the GO modified cysteamine (Cys) self-assembled monolayer (SAM) on an Au electrode (Au/Cys) through the amide bond formation between the carboxylic acid groups of GO/Py-COOH and amine groups of anti-CA 15-3. Secondary antibody conjugated MWCNT-supported ferritin labels (Ab<SUB>2</SUB>-MWCNT-Ferritin) were prepared through the amide bond formation between amine groups of Ab<SUB>2</SUB> and ferritin and carboxylic acid groups of MWCNTs. The detection of CA 15-3 was based on the enhanced bioelectrocatalytic reduction of hydrogen peroxide mediated by hydroquinone (HQ) at the GO/Py-COOH-based sensor probe. The GO/Py-COOH-based sensor probe and Ab<SUB>2</SUB>-MWCNT-Ferritin labels were characterized using cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), scanning electron microscope (SEM), transmission electron microscope (TEM), and x-ray photoelectron spectroscopy (XPS) techniques. Using differential pulse voltammetry (DPV) technique, CA 15-3 can be selectively detected as low as 0.01±0.07U/mL in human serum samples. Additionally, the proposed CA 15-3 immunosensor showed excellent selectivity and better stability in human serum samples, which demonstrated that the proposed immunosensor has potentials in proteomic researches and diagnostics.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Serum-based CA 15-3 immunosensor was fabricated. </LI> <LI> Immunosensor was fabricated using non-covalent graphene oxide probe. </LI> <LI> Enhanced detection was achieved using carbon nanotube supported ferritin labels. </LI> <LI> The catalytic activity of ferritin was utilized for the signal generation. </LI> <LI> Detection limit of CA 15-3 in serum was as low as 0.01U/mL. </LI> </UL> </P>

Application of sweet and taste modifying genes for development in plants: current status and prospects

AKTER SHAHINA,Md. Amdadul Huq,정유진,조용구,강권규 한국식물생명공학회 2016 JOURNAL OF PLANT BIOTECHNOLOGY Vol.43 No.4

Sweet and taste modifying proteins are natural alternatives to synthetic sweeteners and flavor enhancers, and have been used for centuries in different countries. Use of these proteins is limited due to less stability and availability. However, recent advances in biotechnology have enhanced their availability. These include production of sweet and taste modifying proteins in transgenic organisms, and protein engineering to improve their stability. Their increased availability in the food, beverage or medicinal industries as sweeteners and flavor enhancers will reduce the dependence on artificial alternatives. Production of transgenic plants using sweet and taste modifying genes, is an interesting alternative to the extraction of these products from natural source. In this review paper, we briefly describe various sweet and taste modifying proteins (such as thaumatin, monellin, brazzein, curculin and miraculin), their properties, and their application for plant development using biotechnological approaches.

Biological Synthesis of Ginsenoside Rd Using Paenibacillus horti sp. nov. Isolated from Vegetable Garden

Akter, Shahina,Huq, Md. Amdadul Springer-Verlag 2018 Current microbiology Vol.75 No.12

Femtomolar detection of cardiac troponin I using a novel label-free and reagent-free dendrimer enhanced impedimetric immunosensor

Akter, Rashida,Jeong, Bongjin,Lee, Yong-Mi,Choi, Jong-Soon,Rahman, Md. Aminur Elsevier 2017 Biosensors & bioelectronics Vol.91 No.-

<P><B>Abstract</B></P> <P>A novel highly sensitive dendrimer coupled impedimetric immunosensor was developed for the label-free and reagent-free detection of cardiac troponin I (TnI) in serum samples. The immunosensor probe was fabricated by covalently attaching carboxylic acid-functionalized third generation (G3) poly (amidoamine) (PAMAM) dendrimer (Den) on the 3, 3′, 5, 5′-tetramethyl benzidine (TMB) modified 6-mercaptohexanoic acid (MHA) self-assembled monolayer (SAM) on a gold (Au) electrode. Monoclonal anti-TnI antibody was then covalently immobilized on the Den and TMB attached MHA SAM modified surface. TMB was used as an internal surface redox couple for generating signal which also allowed to avoid the use of an external one (<I>i.e.</I> ferricyanide couple) in solution during the impedance measurement for monitoring the antibody-antigen binding. On the other hand, Den was used as a signal enhancer by immobilizing more anti-body on the immunosensor probe. The immunosensor probe was characterized using X-ray photoelectron spectroscopy (XPS), quartz crystal microbalance (QCM), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) techniques. The TnI detection in diluted serum was based on the measurement of charge transfer resistance (<I>R</I> <SUB>ct</SUB>) of the electron transfer process of the surface-attached TMB before and after immunobinding. Under the optimized condition, the proposed immunosensor could detect human TnI in diluted serum samples as low as 11.7 fM with a wide linear dynamic range, good stability, and excellent specificity. The validity of the proposed method was tested in various TnI spiked human undiluted serum samples and was compared with the enzyme-linked immunosorbent assay (ELISA). The results suggested that the proposed immunosensor could be a useful tool for practical applications in clinical diagnosis.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Impedance-based cardiac troponin I immunosensor was fabricated. </LI> <LI> Reagent-less and label-free detection was developed using tetramethylbenzidine. </LI> <LI> Enhanced detection was achieved using Dendrimer at the probe. </LI> <LI> Detection limit of troponin I in a diluted serum was as low as 11.7 fM. </LI> </UL> </P>

Spectral relaxation computation of electroconductive nanofluid convection flow from a moving surface with radiative flux and magnetic induction

Akter Shahina,Ferdows M,Bég Tasveer A,Bég O Anwar,Kadir A,Sun Shuyu 한국CDE학회 2021 Journal of computational design and engineering Vol.8 No.4

A theoretical model is developed for steady magnetohydrodynamic viscous flow resulting from a moving semi-infinite flat plate in an electrically conducting nanofluid. Thermal radiation and magnetic induction effects are included in addition to thermal convective boundary conditions. Buongiorno’s two-component nanoscale model is deployed, which features Brownian motion and thermophoresis effects. The governing nonlinear boundary layer equations are converted to nonlinear ordinary differential equations by using suitable similarity transformations. The transformed system of differential equations is solved numerically, employing the spectral relaxation method (SRM) via the MATLAB R2018a software. SRM is a simple iteration scheme that does not require any evaluation of derivatives, perturbation, and linearization for solving a nonlinear system of equations. Effects of embedded parameters such as sheet velocity parameter$\lambda$, magnetic field parameter$\beta$, Prandtl number$Pr$, magnetic Prandtl number$Prm$, thermal radiation parameter$Rd$, Lewis number$Le$, Brownian motion parameter$Nb$, and thermophoresis parameter$Nt$ on velocity, induced magnetic field, temperature, and nanoparticle concentration profiles are investigated. The skin-friction results, local Nusselt number, and Sherwood number are also discussed for various values of governing physical parameters. To show the convergence rate against iteration, residual error analysis has also been performed. The flow is strongly decelerated, and magnetic induction is suppressed with greater magnetic body force parameter, whereas temperature is elevated due to extra work expended as heat in dragging the magnetic nanofluid. Temperatures are also boosted with increment in nanoscale thermophoresis parameter and radiative parameter, whereas they are reduced with higher wall velocity, Brownian motion, and Prandtl numbers. Both hydrodynamic and magnetic boundary layer thicknesses are reduced with greater reciprocal values of the magnetic Prandtl number Prm. Nanoparticle (concentration) boundary layer thickness is boosted with higher values of thermophoresis and Prandtl number, whereas it is diminished with increasing wall velocity, nanoscale Brownian motion parameter, radiative parameter, and Lewis number. The simulations are relevant to electroconductive nanomaterial processing.

Reinforcement Learning-based Duty Cycle Interval Control in Wireless Sensor Networks

Akter, Shathee,Yoon, Seokhoon The Institute of Internet 2018 Journal of Advanced Smart Convergence Vol.7 No.4

One of the distinct features of Wireless Sensor Networks (WSNs) is duty cycling mechanism, which is used to conserve energy and extend the network lifetime. Large duty cycle interval introduces lower energy consumption, meanwhile longer end-to-end (E2E) delay. In this paper, we introduce an energy consumption minimization problem for duty-cycled WSNs. We have applied Q-learning algorithm to obtain the maximum duty cycle interval which supports various delay requirements and given Delay Success ratio (DSR) i.e. the required probability of packets arriving at the sink before given delay bound. Our approach only requires sink to compute Q-leaning which makes it practical to implement. Nodes in the different group have the different duty cycle interval in our proposed method and nodes don't need to know the information of the neighboring node. Performance metrics show that our proposed scheme outperforms existing algorithms in terms of energy efficiency while assuring the required delay bound and DSR.

Validation of Neurotensin Receptor 1 as a Therapeutic Target for Gastric Cancer

Akter, Hafeza,Yoon, Jung Hwan,Yoo, Young Sook,Kang, Min-Jung Korean Society for Molecular and Cellular Biology 2018 Molecules and cells Vol.41 No.6

Gastric cancer is the fifth most common type of malignancy worldwide, and the survival rate of patients with advanced-stage gastric cancer is low, even after receiving chemotherapy. Here, we validated neurotensin receptor 1 (NTSR1) as a potential therapeutic target in gastric cancer. We compared NTSR1 expression levels in sixty different gastric cancer-tissue samples and cells, as well as in other cancer cells (lung, breast, pancreatic, and colon), by assessing NTSR1 expression via semi-quantitative real-time reverse transcription polymerase chain reaction, immunocytochemistry and western blot. Following neurotensin (NT) treatment, we analyzed the expression and activity of matrix metalloproteinase-9 (MMP-9) and further determined the effects on cell migration and invasion via wound-healing and transwell assays. Our results revealed that NTSR1 mRNA levels were higher in gastric cancer tissues than non-cancerous tissues. Both of NTSR1 mRNA levels and expression were higher in gastric cancer cell lines relative to levels observed in other cancer-cell lines. Moreover, NT treatment induced MMP-9 expression and activity in all cancer cell lines, which was significantly decreased following treatment with the NTSR1 antagonist SR48692 or small-interfering RNA targeting NTSR1. Furthermore, NT-mediated metastases was confirmed by observing epithelial-mesenchymal transition markers SNAIL and E-cadherin in gastric cancer cells. NT-mediated invasion and migration of gastric cancer cells were reduced by NTSR1 depletion through the Erk signaling. These findings strongly suggested that NTR1 constitutes a potential therapeutic target for the inhibition of gastric cancer invasion and metastasis.

Effect of Different Preservation Methods on Physicochemical Quality of Beef

Akter, H.,Akhter, S.,Rahman, S.M.E.,Rahman, M.M.,Hossain, M.M.,Ra, C.S.,Kim, Jai-Moung,Oh, Deog-Hwan The Korean Society of Food Hygiene and Safety 2009 한국식품위생안전성학회지 Vol.24 No.3

본 연구는 쇠고기의 품질에 있어서 건조, 절임, 냉동의 효과를 평가하기 위해 수행되었다. 건조의 3가지 타입(소금 비처리 = $T_1$, 소금 처리 = $T_2$, 소금 및 향료 처리 = $T_3$), 절임의 3가지 타입(소금 절임 = $T_4$, 설탕 처리 = $T_5$, 소금물 처리 = $T_6$)과 냉동의 3가지 타입($0^{\circ}C$ = T7, $-10^{\circ}C$ = T8, $-20^{\circ}C$ = T9)으로 180일 동안 시간 간격을 두고 쇠고기의 단백질, 지방, 회분, 색도와 조리 영양손실을 분석하였다. 모든 화학적 구성요소들(단백질, 지방과 회분)은 120일 까지 점차적으로 감소하였으며 감소 경향은 저장 120일이 지난 후 180일까지 빠르게 진행되는 것으로 관찰되었다. 180일 저장 기간 동안 $T_7$에서 단백질 손실이 11.1%로 가장 높았으며 $T_6$는 3.85%로 가장 낮은 단백질 손실을 나타냈고 다른 처리구들에서도 유의적 차이(p < 0.01)가 나타났다. $T_6$에서 지방 손실이 7.52%로 가장 높았으며 $T_2$에서 3.18%로 가장 낮았다. 또한 다른 처리구들에서도 서로 유의적인 차이(p < 0.05)를 나타내었다. 향료가 처리된 건조 쇠고기가 가장 밝은 색을 나타냈으며 절임 쇠고기는 갈색으로 나타났다 색의 명암은 저장 기간이 증가함에 따라 점차적으로 감소하였다. 3가지 벙동 타입 중 $T_9$ 쇠고기가 가장 낮은 조리 영양손실을 나타냈으며 다른 처리구들 또한 유의적 차이(p < 0.01)를 나타냈다 설탕 절임($T_5$)과 향료처리 건조($T_3$)방법은 농촌 지역에서 육류 저장기술로 유용할 것이며 냉동($T_9$)방법은 도시 지역에서 큰 규모의 저장에 사용되면 좋을것으로 사료된다. The study was conducted to evaluate the effect of drying, curing and freezing on the quality of beef. Three types of dried (without salt = $T_1$, with salt = $T_2$ and salt + spices = $T_3$); three types of cured (salt curing = $T_4$, sugar curing = $T_5$ and brine curing = $T_6$) and three types of frozen beef ($0^{\circ}C=T_7^{\circ}C$, $-10^{\circ}C=T_8$ and $-20^{\circ}C=T_9$) were analyzed at different time intervals up to the period of 180 d. Parameters studied were protein, fat, ash, color and cooking loss of beef. All the chemical constituents (protein, fat and ash) were decreased gradually up to 120 d. The decreasing trend was observed rapid after 120 d up to 180 d of preservation. Highest protein loss was found in $T_7$ (11.1 %) and the lowest protein loss was found in $T_6$ (3.85%) in 180 d preservation and significant (p < 0.01) differences were observed among the different preservation methods. Highest fat loss was observed in $T_6$ (7.62%) and the lowest fat loss was observed in $T_2$ (3.18%) and the differences were also significant (p < 0.05) among different methods during the experimental period. Spices dried beef showed a brighter color than others and cured beef showed brown color and the intensity of color was reduced gradually with the increasing of storage period. $T_9$ showed the lowest cooking loss among 3 treatments of frozen beef and the differences also significant (p < 0.01) up to 180 d. It might be stated that sugar curing ($T_5$) and spices drying ($T_3$) would be the useful technique of meat preservation in rural areas and freezing ($T_9$) would be used in large scale preservation at urban areas.