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Evaluation of In Vitro Antioxidant Activity of Sida rhombifolia (L.) Ssp. retusa (L.)
Ajit P. Purohit,Kamlesh Dhalwal,Yogesh S. Deshpande 한국식품영양과학회 2007 Journal of medicinal food Vol.10 No.4
Sida rhombifolia(L.) ssp. retusa(L.) is widely used in Ayurvedic medicine for the treatment of fever as wellEstimation of total polyphenolic content and high-performance thin-layer chromatography profile were determined. Furtherinhibition of oxygen-derived free radicals, viz., assays for free radical scavenging, reducing power, superoxide anion scav-enging, nitric oxide scavenging, and anti-lipid peroxidation, were performed. All the antioxidant activities were comparedwith standard antioxidants such as butylated hydroxyanisole and .-tocopherol acetate. Extracts were found to be good scav-engers of the 1,1-diphenyl-2-picrylhydraazyl radical in the order root. leaves. whole plant. stem with 50% inhibitory con-.g/mL, respectively. All extracts of this plant showed effective free radicalscavenging activity, reducing power, and superoxide scavenging activity. Only root extract inhibited lipid peroxidation in ratliver and brain homogenate. All these antioxidant properties were concentration dependent. In addition, total polyphenoliccontents of all the extracts were determined as gallic acid equivalents. The highest antioxidant activity was observed in rootextract. The results obtained from the current study indicate that S. rhombifolia ssp. retusais a potential source of natural an-tioxidants.