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Induction of anti-aquaporin 5 autoantibodies by molecular mimicry in mice
Lee, Ahreum,Choi, Youngnim 대한구강생물학회 2020 International Journal of Oral Biology Vol.45 No.4
Molecular mimicry is the most common mechanism that breaches self-tolerance. We previously identified autoantibodies to aquaporin-5 (AQP5) in the sera of patients with Sjögren's syndrome and found that the aquaporin of Prevotella melaninogenica (PmAqp), an oral commensal, is highly homologous to human AQP5. This study aimed to test whether PmAqp can induce anti-AQP5 autoantibodies via molecular mimicry. From the amino acid sequence of PmAqp, an immunizing peptide; i.e., PmE-L, was designed, which contained both the B cell epitope "E" and T cell epitope. C57BL/6 and BALB/c mice were subcutaneously immunized with linear or cyclic forms of PmE-L emulsified in incomplete Freund's adjuvant. The concentrations of the antibodies in sera were measured using enzyme-linked immunosorbent assays. Both linear and cyclic PmE-L induced high levels of antibodies against not only the immunized peptides but also autoantibodies against AQP5E and antibodies against PmE, a Pm homolog of AQP5E. In C57BL/6 mice; however, the cyclic form of PmE-L was more efficient than the linear form in inducing autoantibodies against AQP5E that contained a cyclic epitope. The levels of anti-PmE antibodies and anti-AQP5E autoantibodies showed a strong positive correlation (r = 0.95, p < 0.0005), suggesting molecular mimicry. Collectively, the mice produced anti-AQP5E autoantibodies in response to a PmAqp-derived peptide. This model proved to be useful for studying the mechanisms of autoantibody production by molecular mimicry.
Simple and rapid detection of bacteria using a nuclease-responsive DNA probe
Lee, Kyung Jin,Lee, Wang Sik,Hwang, Ahreum,Moon, Jeong,Kang, Taejoon,Park, Kyoungsook,Jeong, Jinyoung The Royal Society of Chemistry 2018 The Analyst Vol.143 No.1
<P>We demonstrate simple and rapid bacterial detection using a nuclease-responsive DNA probe. The probe consisting of a fluorescent dye and a quencher at the 5′ and 3′ termini, respectively, was designed to be cleaved by nucleases such as endonucleases, exonucleases, and DNases, which are released from bacteria using an optimized lysis buffer. The fluorescence signal of the cleaved DNA probe correlates with the number of Gram-negative <I>Escherichia coli</I> and Gram-positive <I>Staphylococcus aureus</I>, and the detection limit was 10<SUP>3</SUP> CFU for <I>E. coli</I> and 10<SUP>4</SUP> CFU for <I>S. aureus</I>. Moreover, this method is specific for live bacteria and takes just one minute to get the signal including sample collection. These features make the present bacterial detection method a powerful on-site bacterial contamination assay which is simple, rapid, and quantitative.</P>
Lee, Ahreum,Kim, Yong C.,Baek, Keumjin,Alam, Jehan,Choi, Yun S.,Rheu, Yaeeun,Shin, Yoo Jin,Kim, Sungtae,Kim, Hyun-Duck,Song, Yeong W.,Choi, Youngnim TaylorFrancis 2018 Virulence Vol.9 No.1
<P><B>ABSTRACT</B></P><P>Autoantibodies against alpha-enolase (ENO1) are often detected in various infectious and autoimmune diseases. Anti-ENO1 antibody titers were reported to be associated with the severity of periodontitis in patients with rheumatoid arthritis. Because the enolase of the periodontal pathogen <I>Treponema denticola</I> (TdEno) has the highest homology with ENO1 among the enolases of human-associated bacteria, we hypothesized that anti-ENO1 autoantibodies produced during the immune response to TdEno may contribute to the progression of periodontitis and tested it in human and mouse systems. In human subjects with healthy periodontium or chronic periodontitis, a strong positive correlation between the levels of anti-TdEno and anti-ENO1 antibodies was observed. In addition, the purified anti-TdEno antibodies recognized ENO1 as well as TdEno in a dot blot, confirming the cross-reactivity between TdEno and ENO1. However, anti-ENO1 antibody titers were not associated with the severity of periodontitis. To further investigate the role of TdEno in the production of anti-ENO1 antibodies and the progression of periodontitis, mice received an oral gavage of <I>P. gingivalis</I> alone, subcutaneous immunization with TdEno alone, or both <I>P. gingivalis</I> oral gavage and TdEno immunization. Immunization with TdEno induced not only anti-TdEno but also anti-mouse Eno1 (mEno1) antibodies and increased the expression of TNFα in the gingival tissues. However, alveolar bone loss was not increased by TdEno immunization. In conclusion, autoreactive anti-ENO1/mEno1 antibodies that are produced as byproducts during the antibody response to TdEno play a minimal role in the progression of periodontitis in the absence of rheumatoid arthritis.</P>
맥켄지/윌리엄 운동이 과체중 요통환자의 요통과 과체중에 미치는 영향
AhReum Lee,Han Suk Jung,Bo Ram Eo 한서대학교 보완대체의학연구소 2016 Research Journal of Complementary and Alternative Vol.- No.7
Due to rapid changes in the modern civilized society, there is a growing interest in the reduction of physical labor and the improvement of health. For the last few of decades, the prevalence of obesity has been continually on the rise. Overseas studies on obesity-related risk factors suggested that obesity could lead to backache-related diseases. The backache is the most common musculo-skeletal disease among people of all ages, classes and vocations, and might result in excessive costs on a socio-economic front. In this fact-finding survey, this study examined the relation between pain intensity, muscle strength, and weight loss, when William exercise, McKenzie exercise, and aerobic exercise were applied as a regimen for Korean adults who suffered from abdominal obesity and backache. After selecting a total of 60 male and female adults as an experiment group, their individual BMIs were measured to classify the study participants into overweight, obese and severe obese groups, and their personal information such as gender, physical profiles and vocations were analyzed. An analysis was conducted based on the self-administered questionnaire answers including the occurrence cases and periods of backache, treatment types, treatment periods, exercise periods, and sedentary times on chairs and in front of computers. A 12-week intensive exercise program which required the participants to do the regular exercise regimen for one hour three times per week (a ‘Mon/Wed/Fri’ team or a ‘Tue/Thur/Sat’ team), and the participants’ physical changes were measured when the 12-week program is finished. In the trends of soaring job stress and musculoskeletal disorder, it can be said that they affect not only one of the problems but also the physical and mental health problems in care worker. The problem of service mentality can be a triggered, so it is important to find a solution for the health in care worker.
Conformationally resolved structures of jet-cooled acetaminophen by UV–UV hole-burning spectroscopy
Lee, Seung Jun,Min, Ahreum,Kim, Yusic,Ahn, Ahreum,Chang, Jinyoung,Lee, Sang Hak,Choi, Myong Yong,Kim, Seong Keun Royal Society of Chemistry 2011 Physical chemistry chemical physics Vol.13 No.37
<P>The conformational structures of jet-cooled acetaminophen were investigated in the gas phase by resonant 2-photon ionization and UV–UV hole-burning spectroscopy. In contrast to the results from a previous study, two nearly isoenergetic conformers were distinctly found in a supersonic molecular beam expansion and positively identified as the <I>cis</I> and <I>trans</I> isomers of acetaminophen by UV–UV hole-burning spectroscopy. The 0–0 bands of the <I>cis</I> and <I>trans</I> isomers were found at 33518.7 and 33485.6 cm<SUP>−1</SUP>, respectively. The vibronic bands of the two isomers are close-lying and/or partially overlapping due to the small energy difference (33 cm<SUP>−1</SUP>) between the two 0–0 bands. As a consequence, the recorded resonant 2-photon ionization spectrum is highly congested in the low excitation energy region, which develops continuously into a featureless, broadened spectrum in the high energy region.</P> <P>Graphic Abstract</P><P>Two ground state conformational isomers of acetaminophen, paracetamol, in its <I>cis</I>- and <I>trans</I>- forms, have been clearly and positively identified. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1cp21999e'> </P>