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RISS 인기검색어
- 검색키워드
- 저자 : AbulKalam Azad Mandal (검색결과 3 건)
- 무료
- 기관 내 무료
- 유료
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Muthaiya, Maria John,Nagella, Praveen,Thiruvengadam, Muthu,Mandal, AbulKalam Azad 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.2
Tea cell suspension culture is an alternative method for the synthesis of secondary metabolites. For the separation of cells, different concentrations of pectinase were used and a concentration of 0.5% was found to be the optimum concentration for the separation of cells (41.7%) in the culture medium than the other two concentrations (33.3 and 25.0%). The separated cells were cultured in liquid MS medium using different PGR combinations. The time taken for the cells to reach stationary phase, under different PGRs, ranged from 17 to 21 d. The maximum cell density was found in IAA and 2, 4-D medium at 21 d followed by 2, 4-D. Results revealed that the amount of secondary metabolites such as catechins were high with stationary phase when compared to other growth phases (lag and log phases). Different concentrations of shikimic acid (10, 20, and 30 mM) were added to the stationary phase of cell culture in the bioreactor and the secondary metabolite content was analyzed. Synthesis of polyphenols, catechins, caffeine, and other secondary components were high (33.87, 22.85, and 4.66%) with 20 mM shikimic acid treatment than the other two concentrations.
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Maria John Muthaiya,PraveenNagella,MuthuThiruvengadam,AbulKalam Azad Mandal 한국작물학회 2013 Journal of crop science and biotechnology Vol.16 No.2
Tea cell suspension culture is an alternative method for the synthesis of secondary metabolites. For the separation of cells, different concentrations of pectinase were used and a concentration of 0.5% was found to be the optimum concentration for the separation of cells (41.7%) in the culture medium than the other two concentrations (33.3 and 25.0%). The separated cells were cultured in liquid MS medium using different PGR combinations. The time taken for the cells to reach stationary phase, under different PGRs,ranged from 17 to 21 d. The maximum cell density was found in IAA and 2, 4-D medium at 21 d followed by 2, 4-D. Results revealed that the amount of secondary metabolites such as catechins were high with stationary phase when compared to other growth phases (lag and log phases). Different concentrations of shikimic acid (10, 20, and 30 mM) were added to the stationary phase of cell culture in the bioreactor and the secondary metabolite content was analyzed. Synthesis of polyphenols, catechins, caffeine, and other secondary components were high (33.87, 22.85, and 4.66%) with 20 mM shikimic acid treatment than the other two concentrations
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Metabolic Responses of Tea (Camellia sp.) to Exogenous Application of Ascorbic Acid
Amarchand Chordia Murugan,Jibu Thomas,Raj Kumar Rajagopal,AbulKalam Azad Mandal 한국작물학회 2012 Journal of crop science and biotechnology Vol.15 No.1
Effects of exogenous application of ascorbic acid on physiological and metabolic changes in tea were investigated in the present study. The highest improvement in yield was evident in response to regular application (a day after every harvest) of ascorbic acid at 600 ppm followed by 400 ppm. Foliar application of ascorbic acid (600 ppm) at regular intervals enhanced chlorophyll a and b con-tents besides a significant increase in total polyphenols and catechins when compared to the untreated control. Exogenous application of ascorbic acid at alternate harvesting rounds increased the activity of polyphenol oxidase whereas ascorbate peroxidase remained unchanged. An increase in phenylalanine ammonia lyase activity was noticed with foliar application of ascorbic acid irrespective of its concentrations. Theaflavins and thearubigins of made tea showed an increase when ascorbic acid was applied with 400 and 600ppm at alternate intervals. The scores of brewed tea liquor characteristics, viz. infusion, color, strength, and briskness were higher even at the lower concentration of ascorbic acid treatment. Results suggest that foliar application of ascorbic acid (400 ppm) proved to be a useful measure to improve the yield, physiological attributes, and antioxidant properties of tea.
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