Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of Streptomyces coelicolor A3(2)

Ferdous, Nadim,Reza, Mahjerin Nasrin,Emon, Md. Tabassum Hossain,Islam, Md. Shariful,Mohiuddin, A.K.M.,Hossain, Mohammad Uzzal Korea Genome Organization 2020 Genomics & informatics Vol.18 No.3

Streptomyces coelicolor is a gram-positive soil bacterium which is well known for the production of several antibiotics used in various biotechnological applications. But numerous proteins from its genome are considered hypothetical proteins. Therefore, the present study aimed to reveal the functions of a hypothetical protein from the genome of S. coelicolor. Several bioinformatics tools were employed to predict the structure and function of this protein. Sequence similarity was searched through the available bioinformatics databases to find out the homologous protein. The secondary and tertiary structure were predicted and further validated with quality assessment tools. Furthermore, the active site and the interacting proteins were also explored with the utilization of CASTp and STRING server. The hypothetical protein showed the important biological activity having with two functional domain including POD-like_MBL-fold and rhodanese homology domain. The functional annotation exposed that the selected hypothetical protein could show the hydrolase activity. Furthermore, protein-protein interactions of selected hypothetical protein revealed several functional partners those have the significant role for the bacterial survival. At last, the current study depicts that the annotated hypothetical protein is linked with hydrolase activity which might be of great interest to the further research in bacterial genetics.

FUZZY EXPERT SYSTEM FOR CONTROLLING SWAMP TERRAIN INTELLIGENT AIR-CUSHION TRACKED VEHICLE

A. HOSSAIN,A. RAHMAN,A. K. M. MOHIUDDIN 한국자동차공학회 2011 International journal of automotive technology Vol.12 No.5

A fuzzy expert system was used in this study to control an intelligent air-cushion tracked vehicle (IACTV) as it operated in a swamp peat terrain. The system was effective in controlling the intelligent air–cushion vehicle while measuring the vehicle traction (TE), motion resistance (MR), power consumption (PC), cushion clearance height (CCH) and cushion pressure (CP). An ultrasonic displacement sensor, pull-in solenoid electromagnetic switch, pressure-control sensor,microcontroller, and battery pH sensor were incorporated into the fuzzy expert system (FES) to experimentally determine the TE, MR, PC, CCH, and CP. In this study, we provide an illustration of how an FES might play an important role in the prediction of the power consumption of the vehicle’s intelligent air-cushion system. The mean relative error in the actual and predicted values from the FES model with respect to tractive effort, total motion resistance and total power consumption were found to be 5.58 %, 6.78 % and 10.63 %, respectively. For all parameters, the relative error in the predicted values was found to be less than the acceptable limit (10%), except for the total power consumption. Furthermore, the goodness of fit of the predicted values was found to be close to 1.0 as expected and, hence, indicates the good performance of the developed system.

PERFORMANCE MEASUREMENTS OF A TRACKED VEHICLE SYSTEM

A. RAHMAN,A. K. M. MOHIUDDIN,A. HOSSAIN 한국자동차공학회 2011 International journal of automotive technology Vol.12 No.4

To improve crossing ability, the most important performance factor for tracked vehicle systems operating on low-bearing capacity peats, and to minimize income losses that result from downtime and maintenance costs, a vehicle was designed in order to adapt to operating condition changes. This article describes the mobile performance of a novel vehicle with segmented rubber tracks on a low-bearing capacity peat. At an equivalent travelling speed, the novel vehicle’s tractive performance in a variable operating environment caused by changes in terrain cohesiveness and hydrodynamic responses was superior to that of the previous model. The new vehicle, which could be operated on the Sepang peat, showed a tractive effort of 42.2% of the gross vehicle weight in field experiments; the recommended minimum tractive effort is between 30 and 36% of the gross vehicle weight.

Kinematics and nonlinear control of an electromagnetic actuated CVT system for passenger vehicle

Ataur Rahman,Sazzad Bin Sharif,Altab Hossain,A. K. M. Mohiuddin,A. H. M. Zahirul Alam 대한기계학회 2012 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.26 No.7

An electromagnetic actuated continuously variable transmission (EMA-CVT) system is developed by two sets of electromagnetic actuators (solenoid) located on primary and secondary pulley. A set of solenoids are attached to the primary and secondary pulley to develop the attraction and repulsive forces. The relationships between the speed ratio and electromagnetic actuation and clamping force and output torque of the CVT are established based on the kinematics of the EMA-CVT system. A fuzzy logic controller (FLC) is developed to control the EMA precisely based on the feedback of the RPM sensor and slope sensor. The EMA-CVT performance with controller has found 28% more than the performance of the EMA-CVT without controller. The solenoids of the EMA were activated by varying the current supply with the Fuzzy-Proportional-Derivative-Integrator (FPID) to maintain the non-linearity of the CVT in response of the vehicle traction torque demand. Result shows that the solenoid is able to pull the plunger in the desired distance with supply current of 12.5 amp while push the plunger to the desired distance with 14.00 amp current supply to the windings when the vehicle is considered in 10% grade. The acceleration time of the ¼ scale car has been recorded as 5.5 s with the response of drive wheels torque.

Energy efficient electromagnetic actuated CVT system

Ataur Rahman,Sazzad Bin Sharif,A.K.M. Mohiuddin,Mahbubur Rashid,Altab Hossain 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.4

A continuously variable transmission (CVT) system transmits the engine/battery power to the car driving wheel smoothly and efficiently. Several types of CVT already been developed to improve the transmission losses while maintaining acceleration time. However,most of the CVT has some constraints in the actuation mechanism which led us to develop an innovative electromagnetic actuator forCVT. Simplified mathematical equations have been developed for the kinematics analysis of clamping forces of the CVT and electromagneticforces of EMA. The EMA has been developed for ¼ scale car with two sets of solenoid. Each of the two sets has been equippedwith primary and secondary pulleys for pushing and pulling the movable sheave. The solenoid is operated by controlling the supply currentwith a fuzzy logic controller. A simulation based fuzzy logic controller has been introduced here for identifying the desired current ofthe EMA actuation. The experimental results show that the EMA develops electromagnetic forces 301 N for the supply current of 3.37amp, which makes the acceleration time of the car in the range of 2.5~3.5 sec and electromagnetic actuated CVT system highly energyefficient.

Cushion pressure control system for an intelligent air-cushion track vehicle

Altab Hossain,Ataur Rahman,A.K.M. Mohiuddin 대한기계학회 2011 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.25 No.4

This paper presents the control system of cushion pressure for the developed intelligent air-cushion track vehicle (IACTV) for operating on swamp terrain and wet fields. A novel auto-adjusting supporting system is designed for the vehicle’s intelligent air-cushion system. Focusing on minimizing the total power demand of the vehicle, an optimization model has been established, for examining the effects of vehicle parameters and load distribution on power consumption by controlling air-cushion pressure. Then optimum cushion pressure is determined based on the developed optimum pressure – sinkage relationship and the pressure in the cushion chamber is controlled by the Fuzzy controller by maintaining volume flow rate and continuously monitored by the pressure sensor attached with the cushion chamber. The ultrasonic displacement sensor is used to measure the sinkage of the vehicle. The output voltages of the ultrasonic displacement are used to operate the pull-in solenoid switch through the microcontroller which closes the circuit of the compressor motor. Distribution of vehicle load to the air-cushion system is controlled by Fuzzy Logic controller by maintaining the inside pressure of the cushion.