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        TiO<sub>2</sub> Nanoparticles from Baker’s Yeast: A Potent Antimicrobial

        ( Mmk Peiris ),( Tdcp Guansekera ),( Pm Jayaweera ),( Ssn Fernando ) 한국미생물생명공학회(구 한국산업미생물학회) 2018 Journal of microbiology and biotechnology Vol.28 No.10

        Titanium dioxide (TiO<sub>2</sub>) has wide applications in food, cosmetics, pharmaceuticals and manufacturing due to its many properties such as photocatalytic activity and stability. In this study, the biosynthesis of TiO<sub>2</sub> nanoparticles (NPs) was achieved by using Baker’s yeast. TiO<sub>2</sub> NPs were characterized by X-ray Diffraction (XRD), UV-Visible spectroscopy, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM) and Energy Dispersive X-ray analysis (EDX) studies. The XRD pattern confirmed the formation of pure anatase TiO<sub>2</sub> NPs. According to EDX data Ti, O, P and N were the key elements present in the sample. SEM and TEM revealed that the nanoparticles produced were spherical in shape with an average size of 6.7 ± 2.2 nm. The photocatalytic activity of TiO<sub>2</sub> NPs was studied by monitoring the degradation of methylene blue dye when treated with TiO<sub>2</sub> NPs. TiO<sub>2</sub> NPs were found to be highly photocatalytic comparable to commercially available 21 nm TiO<sub>2</sub> NPs. This study is the first report on antimicrobial study of yeast-mediated TiO<sub>2</sub> NPs synthesized using TiCl<sub>3</sub>. Antimicrobial activity of TiO<sub>2</sub> NPs was greater against selected Gram-positive bacteria and Candida albicans when compared to Gram-negative bacteria both in the presence or absence of sunlight exposure. TiO<sub>2</sub> NPs expressed a significant effect on microbial growth. The results indicate the significant physical properties and the impact of yeast-mediated TiO<sub>2</sub> NPs as a novel antimicrobial.

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