지렁이 분변토로부터 황화수소 분해 균주의 분리 및 특성

이은영,조경숙,류희욱,박상진,배무 ( Eun Young Lee,Kyung Suk Cho,Hee Wook Ryu,Sang Jin Park,Moo Bae ) 한국환경생물학회 1997 환경생물 : 환경생물학회지 Vol.15 No.1

A new sulfur -oxidizing bacterium which was capable of degrading hydrogen sulfide was isolated from earthworm casts using deodorant materials. The cell of isolate CP-5 was short rod, gram negative and non-motile, the activities of cytochrome oxidase and catalase were positive. The isolate could autotrophically grow in the basal mineral medium supplemented the reduced sulfur compounds such as thiosulfate and elemental sulfur. This bacterium could oxidize thiosulfate and elemental sulfur to sulfate, and the metabolic intermediates were considered as tetrathionate and thiosulfate, respectively. The removal of hydrogen sulfide by the isolate CP-5 was confirmed, and the CP-5 was regarded as one of microorganisms responsible for degradation of hydrogen sulfide in earthworm casts. The isolate CP-5 can be applied to biodeodorization systems for the improvement of removal efficiency of hydrogen sulfide.

반도체 플라즈마 식각 장치의 부품 가공 연구

이은영,김문기,Lee, Eun Young,Kim, Moon Ki 한국반도체디스플레이기술학회 2020 반도체디스플레이기술학회지 Vol.19 No.4

Plasma etching process employs high density plasma to create surface chemistry and physical reactions, by which to remove material. Plasma chamber includes silicon-based materials such as a focus ring and gas distribution plate. Focus ring needs to be replaced after a short period. For this reason, there is a need to find materials resistant to erosion by plasma. The developed chemical vapor deposition processing to produce silicon carbide parts with high purity has also supported its widespread use in the plasma etch process. Silicon carbide maintains mechanical strength at high temperature, it have been use to chamber parts for plasma. Recently, besides the structural aspects of silicon carbide, its electrical conductivity and possibly its enhanced life time under high density plasma with less generation of contamination particles are drawing attention for use in applications such as upper electrode or focus rings, which have been made of silicon for a long time. However, especially for high purity silicon carbide focus ring, which has usually been made by the chemical vapor deposition method, there has been no study about quality improvement. The goal of this study is to reduce surface roughness and depth of damage by diamond tool grit size and tool dressing of diamond tools for precise dimensional assurance of focus rings.

온라인 소프트웨어교육에서 직장인의 지각된 유용성, 자기조절학습능력이 학습성과에 미치는 영향 -디지털 리터러시, 만족도의 직렬다중매개모형 분석중심-

이은영,Lee, Eun-Young 한국디지털정책학회 2022 디지털융복합연구 Vol.20 No.4

산업전반의 디지털 전환으로 소프트웨어 역량은 미래인재의 핵심역량이 되었다. 하지만 소프트웨어 역량 강화를 위한 기업의 교육과 관련된 연구는 찾아보기 어렵다. 이에 본 연구는 온라인 소프트웨어교육에서 직장인의 학습성과에 영향을 미치는 요인 간의 관계를 검증하고자 하였다. 이를 위하여 온라인 소프트웨어교육 경험이 있는 직장인 223명의 설문을 SPSS PROCESS macro를 사용하여 분석하였다. 분석결과, 지각된 유용성과 자기조절학습능력은 디지털 리터러시와 만족도를 다중매개하여 학습성과에 유의한 영향을 미치는 것으로 나타났다. 이는 학습자 요인뿐만 아니라 교육의 특성도 고려해야 함을 시사한다. 본 연구결과는 효과적인 온라인 교육프로그램을 설계하는 데 도움이 될 것으로 기대된다. With the digital transformation of the entire industry, software competency has become the core competency for the future talent. However, it is difficult to find researches related to the corporate education for improving employee's software capability. Therefore, this study tried to verify the relationship between factors affecting the learning performance of employees in online software education. For this purpose, a survey of 223 employees with online software education experience was analyzed using the SPSS PROCESS macro. As a result of analysis, perceived usefulness and self-regulated learning have been found to have a significant multiple mediating effect on learning performance by digital literacy and satisfaction. This suggests that not only learner factors but also the characteristics of education should be considered. The results of this study are expected to be helpful in designing effective online education programs.

상악골에 발생한 거대 함치성낭종의 적출술 후 장기치료 결과: 증례보고

이은영,김경원,Lee, Eun-Young,Kim, Kyoung-Won 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.1

A long-term follow-up study of a case of impacted teeth associated with a large dentigerous cyst in the left maxilla is presented. The patient was a 30-year-old man who had a large dentigerous cyst in the premaxilla and left posterior maxilla, which impacted the canine and supernumerary tooth. This is one of the most prevalent types of odontogenic cysts associated with an erupted or developing tooth, particularly the mandibular third molars. The other teeth commonly affected in order of frequency are the maxillary canines, maxillary third molars and rarely, the central incisor. Radiographically, the cyst appears as huge ovoid well-demarcated unilocular radiolucency with a sclerotic border and causes ectopic displacement of the inferior border of the maxillary sinus without destruction. Dentigerous cysts may grow unnoticed to such extensive sizes as to occupy a considerable portion of the maxillary sinus. These cysts appear to be associated with a supernumerary tooth in the maxillary anterior incisors region called the mesiodens and impacted canine. The present case report describes the surgical enucleation of a dentigerous cyst involving the permanent maxillary left canine and mesioden. After surgery, left maxillary sinus recovered their normal size and apposition of bone was observed around the apex of the posterior teeth. During the subsequent years, there was no recurrence of the cystic lesion but the inflammation was evoked in the anterior maxilla after 42 months. This complication appeared to have correlated with bony healing in the enucleation site of the cyst. We report the healing status of a huge dentigerous cyst in the maxilla for 5 years with a review of the relevant literature.

동종조직이식술시 전염성질환의 이환가능성에 대한 고찰 II: 동종연조직

이은영,김경원,엄인웅,Lee, Eun-Young,Kim, Kyoung-Won,Um, In-Woong 대한악안면성형재건외과학회 2007 Maxillofacial Plastic Reconstructive Surgery Vol.29 No.3

Implantation of allografts has increased widely with not only the availability of many allogenic bone but also allogenic soft tissues. The aim of tissue banking is to provide surgeons with safe tissues compatible with their intended clinical application. The incidence of tissue transplant-transmitted infection is unknown and can only be inferred from prospective studies. The possibility of donor-to-recipient disease transmission through soft tissue transplantation can be considered by reviewing the risk associated with other transplanted hard tissues. Viral, bacterial, and fungal infections have been transmitted via transplantation of soft tissue allografts such as skin, cornea, dura, pericardium. fascia lata, and heart valves. Corneas have transmitted rabies, Creutzfeldt-Jakob disease (CJD), hepatitis B (HBV), cytomegalovirus (CMV), herpes simplex virus (HSV), bacteria, and fungi. Heart valves have been implicated in transmitting tuberculosis, hepatitis B. HIV-1 and CMV. CJD has been transmitted by dura and pericardium transplants. Skin has transmitted CMV, bacteria, and fungi. Cadaveric skin, pericardium, dura, and fascia lata have been used in dental patients with intra-oral soft tissue injuries and GBR. This study is review of the considering transmission of infectious disease in allogenic soft tissues and guidelines of reducing the risk. Prior to use, many tissues are exposed to antibiotics, disinfectants, and sterilants, which further reduce or remove the risk of transmitted disease. Because some soft tissue grafts cannot be subjected to sterilization steps, the risk of infectious disease transmission remains and thorough donor screening and testing is especially important.

한외여과 공정을 이용한 고순도 향균 Lysozyme 의 분리 및 막 침착 특성

이은영,우건조,Lee, Eun-Young,Woo, Gun-Jo 한국식품과학회 1999 한국식품과학회지 Vol.31 No.2

난백 lysozyme은 박테리아 세포벽을 선택적으로 분해하므로 식품 가공 및 저장용 천연 식품보존제로서의 이용 가치가 높다. 농도(0.25, 0.5, 1.0%, w/v)를 달리한 난백 용액을 예비여과하여 얻은 여액(PFS)과 PFS를 $35^{\circ}C$에서 한외여과하여 얻은 여액(PMS)의 단백질 농도와 lysozyme 농도를 측정하고 비활성도와 purification factor를 계산하여 최적 분리 농도를 결정하였다. 난백 용액의 각 단계별 lysozyme의 분리 정제도를 겔 크로마토그래피와 전기영동으로 확인하였으며, PM30 막에 대한 난백 단백질의 막 침착도는 SEM으로 관찰하였다. 예비여과와 한외여과 단계를 거치면서 lysozyme 이외의 비효소성 단백질은 99% 이상이 제거되었다. 비활성도는 0.25% PMS>0.50% PMS>1.0% PMS 순이었으며 PFS에 비해 PMS의 비활성도는 한외여과 단계를 거치면서 최저 18배에서 최고 31배까지 증가하였으므로, 비활성도와 purification factor가 가장 높은 0.25% 난백 용액을 최적 농도로 결정하였다. 난백 용액(0.25%, w/v)의 겔투과 크로마토그래피 결과 비효소 획분은 대부분 ovalbumin으로 판명되었으며, 전기영동 결과 PMS내에는 고순도의 lysozyme이 존재하였다. 분자량이 큰 단백질들이 $0.9{\mu}m$의 두께로 한외여과 막 표면에 침착되었으나, SEM 관찰 결과 이들 침착 단백질의 대부분은 0.1 N NaOH로 세척시 제거되는 것으로 나타났다. 따라서, polysulfone계의 PM30 막을 사용한 한외여과 공정이 고순도의 lysozyme만을 선택적으로 분리하는데 매우 효과적이었다. The value of lysozyme as a natural food preservative is continuously increased due to its unique antimicrobial activity. To determine the optimum separation concentration among the various hen egg white protein (HEWP) concentrations (0.25, 0.5, 1.0, w/v), protein concentrations, lysozyme concentrations, specific activities (SA), and purification factors of prefiltered solution (PFS) and PM30 permeate solution (PMS) were compared. The purity of lysozyme separated at each step was analyzed and confirmed by gel permeation chromatography and electrophoresis. The fouling deposits on membrane were observed by SEM. The non-enzymatic proteins were removed over 99% by ultrafiltration (UF). The increased feed concentration did not contribute to the increase of SA. SA of PMS was 18 to 31 times higher than that of PFS. The optimum feed concentration was decided as 0.25% based on SA and purification factor. The non-enzymatic region of gel chromatogram was proved to be ovalbumin. The thickness of deposit on the UF membrane was approximately $0.9{\mu}m$ and removed by cleaning with 0.1 N NaOH. Therefore, UF using PM30 membrane was very effective to separate the antimicrobial lysozyme from various HEWPs.

비순 피판을 이용한 상악골 편측 괴사환자의 치험례

이은영,김경원,Lee, Eun-Young,Kim, Kyoung-Won 대한악안면성형재건외과학회 2009 Maxillofacial Plastic Reconstructive Surgery Vol.31 No.2

The maxilla rarely undergoes necrosis due to its rich vascularity. Maxillary necrosis can occur due to bacterial infections such as osteomyelitis. viral infections such as herpes zoster and fungal infections such as mucormycosis, aspergillosis etc. Herpes zoster is a common viral infection, the oral soft tissue manifestations of which are widely known and recognized. Extremely rare complications such as osteonecrosis, and secondary osteomyelitis in maxilla were observed. But, reports of spontaneous tooth exfoliation and jaw osteonecrosis following herpes zoster infection in the distribution of the trigeminal nerve are extremely rare in the literature. We report a case of maxillary necrosis by herpes zoster in an uncontrolled diabetic patient. There was extensive necrosis of the buccal and palatal mucoperiosteum and exposure of the alveolar bone. This patient was successfully treated using a removal of necrotic bone and nasolabial flap. We briefly discuss different diseases which can lead to maxillary necrosis and a review. Analysis of the pathogenesis of herpes zoster and bone necrosis are discussed.

상악골낭종 적출술 후 이식된 이종골지지체의 술 후 8년 감염 증례

이은영,김경원,Lee, Eun-Young,Kim, Kyoung-Won 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.5

Bone grafts are becoming increasingly common in oral and maxillofacial surgery to improve bone healing procedures. Bovine bone as a xenograft is a representative osteoconductor and space filler; however, sometimes complications, such as infection and wound dehiscence are encountered with its use. We report the result of an eight-year follow-up of a xenograft case and processing methods of inorganic bovine bone along with a review of the literature. Xenograft ($LUBBOC^{(R)}$) was used in a cyst enucleation site of the maxilla, as a bone substitute and space filler. Inflammation and infection were defined several times as lack of osseous contact between the graft and host bone, caused by remodeling failure over an eight-year period. Pathologic findings of the xenograft revealed dead bony trabeculae with inflamed fibrous tissue and actinomycosis.

구개부에 발생한 다형성선종의 트랩도어 수술법에 대한 증례보고

이은영,김경원,Lee, Eun-Young,Kim, Kyoung-Won 대한악안면성형재건외과학회 2011 Maxillofacial Plastic Reconstructive Surgery Vol.33 No.6

A 27-year-old female presented to our hospital with a slow growing, hard and soft palate mass on the right that had been present for the several months. Physical examination showed a 2 cm, firm, well-circumscribed, painless mass on the right side of the palate. PNS computer tomographic imaging showed a $1.5{\times}1.3{\times}2$ cm well-defined cystic mass on the right side of both the hard and soft palate without any underlying bone change. The lesion was completely excised under general anesthesia. In order to preserve the palatal mucosa, trapdoor approach for removal of the pleomorphic adenoma was done. This technique provided more comfortable healing of the operative site. Three years after surgery, there was no evidence of recurrence. If pleomorphic adenoma without bony and mucosal destruction exists, we suggest consideration of the trapdoor approach to protect the palatal mucosa. In view of the potential for tumour recurrence, long-term follow-up and careful examination are necessary.

Street Style의 미적 의미에 대한 연구

이은영,Lee, Eun-Young 배재대학교 자연과학연구소 1997 自然科學論文集 Vol.9 No.1

포스트모던 스타일은 현대유행에서 여러 가지로 보여진다. 특히 거리스타일은 테디보이즈, 모즈, 히피, 스킨헤드와 대중유행에서 시작된다. 대중문화는 현대사회에서 여러 가지 방식으로 두드러지고 있다. 음악 연극, 영화에서 젊은이들의 생각을 읽을 수 있는데, 거리스타일은 그들의 공포와 저항 그리고 그들의 열정이 담겨져 있다. 스트리트 스타일의 미학은 대중문화의 미학은 대중문학의 미학에서 다음과 같이 보여진다. ${\cdot}$ 해학과 감상이 원시주의에 ${\cdot}$ 선정성은 비비안 웨스트우드나 잔드라로즈의 작품에서 보여지는 것 같은 에로티즘에 ${\cdot}$ 환상성은 사이버 사이키델릭 패션 속에 표현되어 진다. Postmordern style showed in contemporary fashion is various. Specially, street style begins from teddy boys, mods, hippy, skin head & popular fashion. They are come from mass culture. Mass culture dominated in many ways in modem society. In music, cinema & other plays we can read of youth. Street style includes youth culture about their fear and resistance and their passion. We can see kich in aetheics of street style as it was showed in mass culture. They are as follows. ${\cdot}$ Wit and sympathy expressed in primitivism. ${\cdot}$ Sensuality expressed in erotism like shows in works of Vivian Westwood & Zandra Rhodes. ${\cdot}$ Fantasy expressed cyber & psychidelic fashion.