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이상윤,김동수,남윤권 한국수산과학회 2012 Fisheries and Aquatic Sciences Vol.15 No.3
The marine medaka Oryzias dancena choriogenin H gene (odChgH) and its mRNA expression during estradiol-17β (E2) exposure were characterized. At the amino acid level, the choriogenin H protein is predicted to possess the conserved repetitive N-terminal region, as well as zona pellucida (ZP) and Trefoil factor family (TFF) domains. At the genomic level, odChgH has an eight-exon organization with a distribution pattern of transcription factor binding sites in the 5′-upstream region, which is commonly found in other estrogen-responsive genes. The tissue distribution pattern of odChgH mRNA was found to be gender-specific, whereby females showed a higher expression level and wider tissue distribution than did males. During embryonic development, odChgH mRNA was robustly detected from the stage of visceral blood vessel formation. Experimental E2 exposure of males resulted in odChgH mRNA being induced not only in the liver, but also in other several tissues. The E2-mediated induction was fairly dosedependent. The basal expression levels of hepatic odChgH mRNA were lower in males that were acclimated to 30 ppt salinity than in those acclimated to 0 or 15 ppt salinity. In contrast, the inducibility of odChgH mRNA during E2 exposure was greater in seawater-acclimated fish than in brackish water- or freshwater-acclimated fish.
이상윤,김병수,김동수,남윤권 한국수산과학회 2011 Fisheries and Aquatic Sciences Vol.14 No.2
Two hepcidin paralogs (ojhamp1 and ojhamp2) were isolated and characterized from a euryhaline Javanese ricefish (Oryzias javanicus: Beloniformes). The ojhamp1 cDNA encoded 90 or 91 amino acids (aa) of a typical HAMP1 preproprotein. This preproprotein is believed to cleave and yield the 66 or 67 aa-proprotein, followed by the 26 aa-mature peptide, composed of 8 conserved cysteine residues and the QSHL amino terminal motif. The ojhamp2 cDNA encoded 89 aa of HAMP2 preproprotein, cleaved to yield a 65 aa proprotein, and subsequently the 25 aa-mature peptide. The mature OJHAMP1 possessed a cationic isoelectric point (pI), whereas OJHAMP2 had an anionic charge. At the genomic level, both ojhamp1 and ojhamp2 share a conserved tripartite structure (three exons interrupted by two introns) with other vertebrate hepcidin genes. However, the ojhamp1 was shown to exist as two distinct mRNA species, encoding 90 or 91 aa, due to alternative splicing at the junction site between intron I and exon II. Both ojhamp1 and ojhamp2 transcripts were detected in a wide range of tissue types with varying levels of basal expression, although the highest expression was observed in the liver for both isoforms. Transcriptional response to bacterial challenge using Edwardsiella tarda showed that ojhamp1 was moderately upregulated in the liver but remained unchanged in the kidney. However, the ojhamp2 was significantly suppressed in both the kidney and liver, suggesting a potential diversification between the two paralogs.
이상윤,김동수,남윤권 한국수산과학회 2012 Fisheries and Aquatic Sciences Vol.15 No.4
We characterized the cytoskeletal beta-actin (β-ACT) gene (actb) and its 5´-upstream regulatory region in the Javanese ricefish Oryzias javanicus. The gene and protein structures were deduced from amino acid sequences of the actb gene and conserved in the teleost lineage. The O. javanicus actb gene has common transcription factor binding motifs in its regulatory region found in teleostean orthologues. Following quantitative reverse transcription-PCR, actb gene transcripts were detected in all tissues examined; however, the basal expression levels were different. During early development, O. javanicus actb mRNA levels showed a gradual increase and peaked between late somitogenesis and the heartbeat stage. Microinjection of O. javanicus embryos with the actb gene promoter-driven red fluorescent protein (RFP) gene reporter vector showed a ubiquitous distribution of RFP signals, although most exhibited a mosaic pattern of transgene expression. A small number of microinjected embryos displayed a wide distribution of RFP signals over their entire body, which resembled the expression pattern of endogenous actb. Data from this study provide a basis to develop a transgenic system with ubiquitous expression of foreign genes in O. javanicus.
이상윤,이승훈 에스케이텔레콤 (주) 2014 Telecommunications Review Vol.24 No.6
광대역 기술발전, 서비스 수요증대로 국내외에서 광대역 재난안전통신망 구축에 대한 관심이 증대하고 있다. 사고발생 시 주파수 소요가 급증하는 재난안전통신 시스템의 특성을 고려해 특정 응급상황의 트래픽 이용 시나리오를 가정해 주파수 소요량을 산출하였으며 그 결과 2x10MHz폭으로 서비스가 가능할 것으로 예측되었다. 또한 주파수 이용이적은 평시 상황에서 효율적인 주파수 이용을 위해 고속철도, 연안 선박을 위한 공공 광대역 서비스 제공을 고려할 수있으며 이 경우에도 2x10MHz폭 범위 내에서 서비스가 가능할 것으로 예측된다. 평시에 비해 재난 시에는 트래픽 폭주가 발생할 수 있는데 이 경우를 대비한 용량 확보 방안으로서 이동 기지국, 네트워크 공유 등의 방안을 고려할 수 있으며 점대점, 메쉬망 등을 통해 트래픽 분산이 가능한 신규 주파수 대역 분배 검토가 필요하다.
李相潤,李相杰 慶北大學校 1989 論文集 Vol.47 No.-
We have fabricated the double insulated white color emitting thin film electroluminescent devices with S_rS: Ce, K, Eu under different fabrication conditions such as the concentration of dopants, substrate temperature and annealing process. Also, we investigated the electrical and optical properties of devices which depend on fabrication conditions and the roles of buffer layer. The crystal structure of as deposited films and annealed films with XRD was measured. The white light(450~700nm) emission was observed and emission brightness B was measured as the function of applied voltage. The maximum brightness of desices was 700 cd/㎡ under 5kHz sinusoidal voltage excitation.