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이춘환,김경숙,Robert t . Ross ( Choon Hwan Lee,Kyung Sook Kim,Robert T . Ross ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.4
The information contained in vivo fluorescence spectra of biological specimens is often obscured by severe overlapping of component spectra. Fluorescence intensity is separately linear in functions of excitation wavelength, emission wavelength, and any chemical treatment which alters overall fluorescence yield. When data are collected for several values of each of these three independent variables, strongly overlapping spectra can be resolved without the use of any a priori information about their shapes. Using concentration of a fluorescence quencher, potassium iodide, as a third variable, a mixture of rhodamine 6G, rhodamine B, and sulforhodamine 101 was used as a test system; the spectra resolved agreed closely with the spectra of the pure dyes. Previously published applications of trilinear analysis to luminescence spectroscopy have required either an instrument capable of time resolution or multiple specimens with varying relative concentrations of fluorophores; use of quencher concentration as a variable permits use of the method with a steady-state fluorometer and without changing fluorophore concentrations. Requiring that the spectra deduced be non-negative can increase the accuracy of the results.
이춘환,이진범,장호식,문병용,정익교,전현식,이진애 동의대학교 기초과학연구소 1993 基礎科學硏究論文集 Vol.3 No.1
The inhibitory effects of mercury ions on the growth of barley seedlings were studied and the distribution of metal elements in the organs of treated plants was investigated by using synchrotron radiation induced X-ray emission (SRIXE). Although the treatment of mercury ions caused growth inhibition, the mercury-specific increase in variable fluorescence and the abolishment of energy-dependent quenching in broken barley chloroplasts as shown by Moon et al.(1992) were not observed in the leaves of growth-inhibited seedlings. Instead the treatment of mercury decreased Fmax and Fo values. However, Fmax/Fo ratio and photochemical and nonphotochemical quenching coefficients were not affected significantly. By SRIXE analysis of 10μM mercury chloride treated seedlings, accumulation of mercury in roots was observed after 1 hour of treatment and similar concentration was sustained for 48 hours. Relative contents of mercury was high in roots and underground nodes where seeds were attached, but was very low in leaves. Iron and zinc were also distributed mainly in the lower parts of the seedlings. However after 72 hours of treatment the contents of these metals in roots decreased and their distribution became more uniform, which may lead to death of the plants. These results suggest that the observed inhibitory effects on barley seedlings upto 48 hours after the treatment is not due to direct damages in the photosynthetic apparatus, but due to its accumulation in roots and the consequent retardation of the growth of barley seedlings. The decrease in Fmax and Fo is probably due to the decrease in chlorophyll and protein contents caused by the retardation of growth. The observed slow expansion of primary leaves could be also explained by the retardation of growth, but the fluorescence induction pattern from the leaves did not show characteristic symptoms of leaves under water stress.
이춘환,장호식,정익교,전현식,이진범,문병용,이진애 한국환경과학회 1992 한국환경과학회지 Vol.1 No.1
The inhibitory effects of mercury ions on the growth of barley seedlings were studied and the distribution of metal elements in the organs of treated plants was investigated by using synchrotron radiation induced X-ray emission (SRIXE). Although the treatment of mercury ions caused growth inhibition, the mercury-specific increase in variable fluorescence and the abolishment of energy-dependent quenching in broken barley chloroplasts as shown by Moon et al. (1992) were not observed in the leaves of growth-inhibited seedlings. Instead the treatment of mercury decreased Fmax and Fo values. However, Fmax/Fo ratio and photochemical and nonphotochemical quenching coefficients were not affected significantly. By SRIXE analysis of 10μM mercury chloride treated seedlings, accumulation of mercury in roots was observed after 1 hour of treatment and similar concentration was sustained for 48 hours. Relative contents of mercury was high in roots and underground nodes where seeds were attached, but was very low in leaves. Iron and zinc were also distributed mainly in the lower parts of the seedlings. However after 72 hours of treatment the contents of these metals in roots decreased and their distribution became more uniform, which may lead to death of the plants. These results suggest that the observed inhibitory effects on barley seedlings upto 48 hours after the treatment is not due to direct damages in the photosynthetic apparatus, but due to its accumulation in roots and the consequent retardation of the growth of barley seedlings. The decrease in Fmax and Fo is probably due to the decrease in chlorophyll and protein contents caused by the retardation of growth. The observed slow expansion of primary leaves could be also explained by the retardation of growth, but the fluorescence induction pattern from the leaves did not show characteristic symptoms of leaves under water stress.
이춘환 한국생화학분자생물학회 1990 생화학분자생물학회 소식 Vol.10 No.3
Photosynthesis research fields using chlorphyll fluorescence as a very sensitive and non-destructive tool are introduced. Steady-state fluoresence can be studied by using derivative and curve-fitting analyses. In comparison to these methods, multilinear analysis is introduced as a new tool which can handle broad room-temperature fluorescence spectra of photosynthetic systems very well. Fluorescence induction process is divided into the fast rising period and the next slow decaying period. Information available by studying these periods and limitations on several methods employed in studying photosystem Ⅱ heterogeneity are discussed. Finally studies on time-resolved fluorescence using single-photon timing method are introduced with emphasis on the global analysis and the target analysis.