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Production and Breeding of Transgenic Cloned Pigs Expressing Human CD73
이승찬,이해선,오근봉,황인설,양혜온,박미령,옥선아,우재석,임기선,황성수 한국발생생물학회 2017 발생과 생식 Vol.21 No.2
One of the reasons to causing blood coagulation in the tissue of xenografted organs was known to incompati-bility of the blood coagulation and anti-coagulation regulatory system between TG pigs and primates. Thus, overexpression of human CD73 (hCD73) in the pig endothelial cells is considered as a method to reduce coagulopathy after pig-to-non-human-primate xenotransplantation. This study was performed to produce and breed transgenic pigs expressing hCD73 for the studies immune rejection responses and could provide a successful application of xenotransplantation. The transgenic cells were con-structed an hCD73 expression vector under control porcine Icam2 promoter (pIcam2-hCD73) and established donor cell lines expressing hCD73. The numbers of transferred reconstructed embryos were 127 ± 18.9. The pregnancy and delivery rate of surrogates were 8/18 (44%) and 3/18 (16%). The total number of delivered cloned pigs were 10 (2 alive, 7 mummy, and 1 died after birth). Among them, three live hCD73-pigs were successfully delivered by Caesarean section, but one was dead af-ter birth. The two hCD73 TG cloned pigs had normal reproductive ability. They mated with wild type (WT) MGH (Massachu-setts General Hospital) female sows and produced totally 16 piglets. Among them, 5 piglets were identified as hCD73 TG pigs. In conclusion, we successfully generated the hCD73 transgenic cloned pigs and produced their litters by natural mating. It can be possible to use a mate for the production of multiple transgenic pigs such as α-1,3-galactosyltransferase knock-out /hCD46 for xenotransplantation.
응애류의 약제 저항성에 관한 연구 II . Metasystox, Folidol 및 C-8514에 대한 과수응애류의 저항성과 방제시험
이승찬,유재기,Lee S. C.,Yoo J. K. 한국응용곤충학회 1971 한국식물보호학회지 Vol.10 No.2
약제 저항성 응애류를 효율적으로 방치하기 위하여 지역별 응애계통의 약제 저항성 수준을 조사하고 유기인제계의 저항성 응애계통에 대하여 화학적 group이 다른 Alternative acaricide로 방제 시험한 결과 1. 유기인제계 (Metasystox 및 Folidol)와 유기염소제계 (C-8514)에 대한 지역 별 응애계통의 저항성 정도를 보면. 가. Metasystox에 대한 사과응애는 인주감수성 계통에 비하여 충주계통이 96배 대구계통이 52배 예산계통이 4배 춘천계통이 3배 수원계통이 2배 가량의 저항성이 생겼고 점박이응애는 감수성계통에 비교하여 대구계통이 32배, 춘천계통이 29배, 예산계통이 25배, 그리고 수원계통이 17배의 저항성을 보였다. 나, Folidol에 대한 사과응애는 감수성계통에 비교하면 충주계통은 126배, 춘천계통은 48배, 예산계통은 33배, 화성계통은 30배 그리고 수원계통이 6배의 저항성이 생겼다. 다. C-8514에 대한 점박이응애 계통은 오산 감수성 계통에 비교하여 대구 계통이 59배 충주계통이 29배 그리고 경산계통이 19배의 저항성을 보였으며 사과응애는 춘천 감수성계통에 비교하여 대구계통이 42배, 수원 및 화성계통이 31배, 그리고 충주계통이 29배의 저항성을 나타냈다. 2. 유기인제계 저항성 사과응애에 대한 교체약제의 방제시험 결과 유기염소제계인 Kelthane MF,주석제인 Plictran, Carbonate계인 Morestan 그리고 혼합제인 K 114의 효과가 우수하였다. The study involved determination of resistance levels of spider mites to Metasystox, Folidol and C-8514 using slide dip technique: a feid trial of alternative acaricides using an O/P resistant strain. 1. Resistant strains of two-spotted spider mite( Tetranychus urticae Koch) were collected from Taegu, Kyungsan, Chunchun, Yesan, Suwon, Osan and Chungju, and kept in the glasshouse through the experiments. Resistant strains of European red mite (Panonychus ulmi Koch) were collected from Chungju, Taegu, Yesan, Chunchun, Suwon and Whasung, and Wonju susceptible was collected. Immediately after collection, tests were applied. All strains except susceptible populations had regularly been sprayed with Organo-phosphates including Parathion (or Folidol), Metasystox, Phenkapton and Trithion for more than the ten Bast years; and Organo-chlorines such as Kelthane and C-8514 more than five years. Comparisons of the resistant strains and susceptible strains at the $LC_50$ values are as follows: a. With Metasystox, resistance levels of the Chungju, Taegu, Yesan, Chunchun and Suwon resistant strains of P. ulmi were respectively, 96,52,4,3 and 2, times as resistant as the Wonju susceptible strain. b. With Folidol, resistance levels of the Chungju, Chunchun, Yesan, Whasung and Suwon strains of P. ulmi showed respectively, 126, 48, 33, 30 and 6-fold resistance levels over the susceptible strain. c. With C-8514, resistance levels of the Taegu, Suwon, Whasung and Chungju strains of P. ulmi were 42, 31, 30 and 20 times greater than the susceptible strain, respectively. d. With Metasystox, resistance levels of the Taegu, Chunchun, Yesan and Suwon, strains of T urticae were respectively, 32,29,25 and 17 times as resistant as the susceptible strain. e. With C-8514, resistance levels of the Taegu, Chungju and Kyungsan strains of T. urticae showed respectively, 59, 29 and 19-fold resistance levels over the Osan strain. 2. Field trials were carried out to assess the toxicities of eleven alternative materials which would be used for control of O/P resistant strain of Panonychus ulmi. The acaricide groups represented were 2 Organo-chlorines (Kelthane MF and Prethylen), 1 carbamate (Furadan), 1 nitrophenyl (Morocide), 1 carbonate (Morestan), 1 tin(Plictran), 1 fluorine (Nissol), 2 mixtures (Fundal and Banmite) and Organo-phosphate (Phenkapton). Of all acaricides tested Kelthane, Plictran, Fundal and Morestan were the most effective; fol]owed by Banmite, Furadan, Prethylen and Nissol. Sumite and Morocide were intermediate, but Phenkapton(Organo-phosphate) was very poor. In other words, a first application of the above outstanding materials gave very high degrees of control of O/P resistant population of European red mite for about 7 to 12 days. However, the results indicate that secondary application would sometimes be necessary. There was no foliage damage to apple trees by any of the acaricides tested of the concentrations used.