한국(韓國)의 건강인(健康人)에서 간염(肝炎) B항원(抗原)(HBsAg) 양성율(陽性率)에 대한 조사연구(調査硏究)

이수영,김강석,Lee, Soo-Young,Kim, Kang-Sueck 한국생명보험의학회 1984 保險醫學會誌 Vol.1 No.1

In the 2,218 healthy cases-male 1,574 female 644-who were conducted serum examination from May 1st 1983 to November 1st 1983 by the medical selection of Dae Han Kyouk life insurance Co., the results which investigated the HBsAg by using the Reverse Passive Hemagglutination(RPHA) method are as follows; 1. The number of 167 from 2,218 cases proved to be positive, showing 7.52%, the number of 143 from 1,574 male positive, showing 9.08%, and the number 24 from 644 female positive, showing 3.73%. The positive rate of the male was indicated by 5.36% higher than that of the female as the former marked 2.4 times higher distribution than the latter. 2) The positive rate by the type of occupations appears as follows; The highest positive rate group is that of discharger from military service in 1983, the number of 70 from 736 persons marking 9.51% and the next is the merchant 8.79%(8/91), employee of a company markes 8.53%(44/516), high school boy 7.69%(19/247), house wife 5.71%(10/210), carrier woman 3.45%(10/290) and the group of the high school girl records the minimum rate of all, 3.13%(4/128). 3. The positive rate by the age group appears as follows; The highest rate group is that of 31 to 35 years, marking 9.80% positive rate, and the next is $26{\sim}30$(9.3%), $21{\sim}25$(8.01%), $41{\sim}45$(6.96%), $36{\sim}40$(6.9%), those below 20(5.99%), 46 to 50 years shows the minimum rate of all(2.94%) Among the male case, 26 to 30 years shows the highest positive rate by 10.59% and female, 31 to 35 years shows the highest rate by 7.89%. 4. The positive rate by the blood type represents as follows; Blood type A shows 7.30%, type B 7.17%, type O 6.11% and type AB indicates 5.88% in order.

생명보험(生命保險) 계약자(契約者)에서 B형간염(型肝炎) 표면항원(表面抗原)과 표면항체(表面抗體) 양성율(陽性率) 조사(調査) 연구(硏究) : vaccine접종(接種) 이후(以後)의 변화(變化) 양상(樣相)

이수영,이화숙,김경희,김강석,Lee, Soo-Young,Lee, Hoa-Suk,Kim, Kyeong-Hee,Kim, Kang-Seuck 한국생명보험의학회 1986 保險醫學會誌 Vol.3 No.1

To investigate the Positive rate of anti HBs and HBs Ag positive rate of the each group of vaccinated hepatitis B type and not-vaccinated, male 4150 female 2475, from May 1, 1984 to November 30, 1985 in the Medical department of Dae han Kyoyuk ins. Co., We examined into the HBsAg and Anti HBs by using the RPHA method and PHA method and the results which were adjusted statistically were as follows. 1. HBs Ag positive rate was 9.0%(228/2531)in 1984 and 7.0%(287/4069) in 1985, the positive rate in 1985 represented 2% lower than in 1984. 2. Anti HBs positive rate was 43.3%(1096/2531) in 1984 and 42.8%(1744/4069) in 1985, there was no significant change for two years. 3. Anti HBs positive rate in the group of the not-vaccinated hepatitis B type was 39.2%(869/2215) in 1984 and 38.8%(1333/3432)in 1985. 4. Anti HBs positive rate in the group of the vaccinated hepatitis B type more than once was 64.7%(419/647)in male and 71.5%(219/306) in female that was 66.9%(638/953) in total. 5. Anti HBs positive rate in the group of the vaccinated hepatitis B type three times was 68.7%(270/393) in male and 80.0%(156/195) in female and 72.4% in total. In this conclusion; The formation of Anti HBs in female showed more or less higher than male. 6. The cases which were detected HBs Ag and anti HBs at the same time were 4 in male and 3 in female and in the group of the vaccinated hepatitis B type, the number of HBs Ag positive cases were 8 in male and 10 in female. (On condition that we didn't distinguish $5{\mu}g/ml$, hepatitis B type vaccine, from $20{\mu}g/ml$ and after vaccinating, the lapsed time was not settled.)

혈장 혈액응고인자 XIII(프로트란스글루타미나제)의 비촉매 사슬의 반감기

이수영,박란숙,Lee, Soo-Young,Park, Lan-Sook 생화학분자생물학회 1982 한국생화학회지 Vol.15 No.4

혈장혈액응고인자 XIII은 $a_{-2}b_{-2}$형의 효소원으로서, 트롬빈에 의해 활성화되어 효소형인 transglutaminase가 된다. transglutaminase는 ${\epsilon}-({\gamma}$-glutamyl) lysine cross-link를 통한 피브린의 cross-link반응을 촉매한다. $a_{-2}b_{-2}+b_{-2}*{\rightleftharpoons}a_{-2}b_{-2}*+b_{-2}$반응이 일어남을 in vivo 및 in vitro에서 확인하였고, $a_{-2}$ 결핍토끼에서의 $b_{-2}$의 반감기는 4.5시간이었다. 이 사실은 순환혈액속에서 $a_{-2}b_{-2}$형만이 오래 머무를 수 있음을 보여주었다. Rabbit plasma blood coagulation factor XIII(protransglutaminase), like the human zymogen, is a tetramer $(a_{-2}b_{-2})$ as is the enzyme$(a_{-2}b_{-2})$(transglutaminase, E.C. 2.32 13) formed by thrombin activation in the absence of $Ca^{2+}$. Transglutaminase from its zymogen, blood coagulation factor XIII, catalyzes the covalent intermolecular polymerization of fibrin through formation of ${\epsilon}-({\gamma}$-glutamyl) lysine crosslink. Since $a_{-2}b_{-2}+b_{-2}*{\rightleftharpoons}a_{-2}b_{-2}*+b_{-2}$ occurs both in vitro and in vivo, the half-life of $b_2$ in $a_2$-deficient rabbit is 4.5 hours. These results suggest that only tetramer form$(a_{-2}b_{-2})$ of a plasma coagulation factor XIII could sustain longer in blood plasma.

혈장 세로토닌과 외상후 스트레스 장애 : 월남전 참전 재향군인을 대상으로

이수영,강석훈,정문용,이명희,김태용,소형석,정혜경,최진희,Lee, Soo-Young,Kang, Suk-Hoon,Chung, Moon-Yong,Lee, Myung-Hee,Kim, Tae-Young,So, Hyung-Seok,Chung, Hae-Kyung,Choi, Jin-Hee 대한불안의학회 2009 대한불안의학회지 Vol.5 No.1

Objective : The objective of this study was to examine the relationship between plasma serotonin concentration and posttraumatic stress disorder (PTSD) symptoms in chronic PTSD patients who have been taking medication. Methods : Plasma serotonin level of 14 PTSD patients and a control group of 28 Vietnam War veterans was measured by HPLC (high performance liquid chromatography). The Combat Exposure Scale (CES), Mississippi Scale for Combat-Related Posttraumatic Stress Disorder (M-PTSD), Clinician Administered PTSD Scale (CAPS), Hamilton Rating Scale for Depression (HRSD), and Hamiltion Anxiety Scale (HAS) were used to evaluate PTSD symptom severity. Results : Serotonin level was significantly higher in the PTSD group than in the control group (p=0.036, p=0.006, respectively). M-PTSD (p<0.001), CAPS (p<0.001), HRSD (p<0.001), and HAS (p<0.001) scale scores were significantly higher in the PTSD group than in the control group; however, the CES score failed to show a significant improvement (p=0.964). There were no significant differences between plasma serotonin and PTSD symptoms. Conclusion : In chronic PTSD patients who have been taking medications, we can not predict treatment effect and symptom severity by measuring only plasma serotonin levels. PTSD is a complicated disorder which may likely be related to a variety of neurotransmitter systems. Therefore, further research which investigate relationships with norepinephrine, dopamine, and other neurotransmitters as well as serotonin is needed to improve the treatment of PTSD.

이질적 환경을 가지는 백신연구에서 백신효과 추정 방법의 비교연구

이수영,이재원,Lee, Soo-Young,Lee, Jae-Won 한국통계학회 2010 Communications for statistical applications and me Vol.17 No.2

백신연구에서 백신효과를 추정하기 위해 생존시간이 반복 관측되었지만 해석의 용이함과 모형의 편리성 때문에 일반적으로 첫 사건만을 고려한 비례위험모형을 사용해왔다. 그러나 이러한 방법은 실험체들의 감수성과 질병에 대한 노출정도가 이질적인 경우 정보의 손실을 초래할 뿐만 아니라 편향된 결과를 도출한다. 또한 반복 측정된 자료가 서로 독립적이기 보다 상호 연관되어 있을 가능성을 배제할 수 없다. 그러므로 본 연구에서는 시뮬레이션 연구를 통해 다양한 요소가 혼합된 복합적 상황에서 여러 통계적 모형을 이용하여 백신효과를 추정하는 방법들을 비교하였다. In vaccine research, proportional hazards model including only first event have been widely used for estimating vaccine efficacy because it is easy to interpret and convenient. However, this method causes not only loss of information but also biased result when heterogeneity of study subject in exposure and susceptibility exists. Furthermore, it is hard to ignore the possibility that each event is correlated with each other in the repeated events. Therefore, we compare various statistical models to estimate vaccine efficacy under various situations with heterogeneity and event dependency.

가상환자 데이터세트를 기반으로 악관절과 심미를 고려한 진단 및 치료계획 수립

이수영,강동휘,이도연,김희철,Lee, Soo Young,Kang, Dong Huy,Lee, Doyun,Kim, Heechul 대한심미치과학회 2021 Journal of the Korean Academy of Esthetic Dentistr Vol.30 No.2

가상 환자 데이터 세트는 단일 환자로부터 획득한 구강스캔 안면스캔 전신스캔 하악운동경로데이터 등 다양한 소스의 진단 데이터를 하나의 3차원 좌표계로 정렬한 데이터의 집합이다. 치과의사는 가상 환자 데이터 세트를 사용하여 효과적으로 치료 계획을 수립하고 다양한 치료 계획을 가상공간상에서 시뮬레이션 할 수 있으며, 가상 환자 데이터 세트에서 환자의 미소를 디자인 후 그 결과를 시뮬레이션하고 최적의 치료결과를 선택할 수 있다. 가상공간에서 선택된 치료 계획은 3D 프린팅, 밀링, 사출 성형과 같은 제조 기술을 사용하여 환자에게 동일하게 전달될 수 있다. 이러 치료 계획의 전달은 임시 수복물 제작 및 환자의 구강 내에서 목업 확인을 통해 최종 보철물 제작으로 연결할 수 있다. 이와 같이 진단 데이터, 중첩 및 가공의 정확도가 보장된다면 3차원 가상공간 상에서 시뮬레이션된 3D 디지털 스마일 디자인을 실제 환자에게 정확하게 전달할 수 있다. 가상환자데이터세트의 임상적용방법으로 동기능적교합측정 검사를 통해 교합조정치료를 치료계획에서 배제할수 있는 의사결정방법과, 턱관절질환을 가지고 있는 청소년기 특발성 척추측만증 환자의 턱관절 치료전후 전신스캔 비교분석방법, 그리고 전악수복증례인 상하악 총의치환자 진료시 가상환자데이터세트에 기반한 교합평면분석 및 디지털심미분석방법을 제시하였다. The virtual patient dataset is a collection of diagnostic data from various sources acquired from a single patient into a coordinate system of three-dimensional visualization. Virtual patient dataset makes it possible to establish a treatment plan, simulate various treatment procedures, and create a treatment planning delivery device. Clinicians can design and simulate a patient's smile on the virtual patient dataset and select the optimal result from the diagnostic process. The selected treatment plan can be delivered identically to the patient using manufacturing techniques such as 3D printing, milling, and injection molding. The delivery of this treatment plan can be linked to the final prosthesis through mockup confirmation through provisional restoration fabrication and delivery in the patient's mouth. In this way, if the diagnostic data superimposition and processing accuracy during the manufacturing process are guaranteed, 3D digital smile design simulated in 3D visualization can be accurately delivered to the real patient. As a clinical application method of the virtual patient dataset, we suggest a decision-making method that can exclude occlusal adjustment treatment from the treatment plan through the digital occlusal pressure analysis. A comparative analysis of whole-body scans before and after temporomandibular joint treatment was suggested for adolescent idiopathic scoliosis patients with temporomandibular joint disease. Occlusal plane and smile aesthetic analysis based on the virtual patient dataset was presented when treating patients with complete dentures.

구강스캐너와 급속 지르코니아 소결을 이용한 당일 풀지르코니아 보철수복

이수영,Lee, Soo Young 대한심미치과학회 2016 Journal of the Korean Academy of Esthetic Dentistr Vol.25 No.2

Single visit monolithic restoration can be proceed with digital workflow which consist of intraoral scanning, dental CAD(computer aided design) and restoration milling with CAM(Computer aided manufacturing). While zirconia has more than 900MPa of flexural strength compared with 400MPa for lithium disilicate, shortened fabricating time of lithium disilicate is considered to be a better choice for fabricating single visit full contour monolithic restoration. However, new zirconia materials which are TZI C(Dentsply Sirona) and LUXEN Enamel(Dental Max), new induction heating method of sintering furnace, and new sintering protocols for MoSi2 heating elements sintering furnace offer significantly reduction of full contour monolithic zirconia restoration fabrication time with greater translucency. These new developments lead single visit zirconia restoration in reality. 당일수복 보철물 제작을 위해 디지털 프로세스 사용은 필수적이다. 이러한 디지털 프로세스는 구강스캔, 컴퓨터를 이용한 보철물을 디자인 과정(CAD)과 보철물을 가공하는 과정(CAM)을 포함한다. 지르코니아의 굴곡강도는 900Mpa로 리튬디실리케이트의 400MPa보다 더 단단하지만, 리튬디실리케이트를 이용한 보철물 기공시간이 지르코니아와 비교하여 더 짧기에 당일수복 보철물 제작시 리튬디실리케이트 사용이 선호되었다. 하지만, TZI C 와 LUXEN Enamel 와 같은 새로운 소재의 지르코니아가 출시되고, 새로운 소결방식이 개발되어 지르코니아 기공시간을 단축시킬수 있게 되었고, 높은 투명도의 풀지르코니아 크라운을 짧은 시간 안에 제작할 수 있게 되었다. 이러한 발전은 당일 풀지르코니아 보철수복을 현실로 이끌고 있다.

구치부 심미수복, 풀지르코니아 크라운의 파절원인과 그 해결방안

이수영,Lee, Soo Young 대한심미치과학회 2014 Journal of the Korean Academy of Esthetic Dentistr Vol.23 No.2

There are several factors affected fractures of full contour zirconia (FCZ) dental prosthesis in laboratory process. First, residual moisture can cause zirconia cracks. Complete dry is requisite before zirconia sintering to prevent zirconia cracks. Second, slow cooling rate is essential to prevent cracks during zirconia sintering process. Cracks in bridge pontic area, thick dental implant prosthesis can be prevented by slow cooling rate such as 3 degree Celsius per minute during zirconia sintering. Third, slow heating rate and slow cooling rate during staining and glazing procedure is necessary to inhibit thermal shock of sintered dental zirconia. Lower preheat temperature of porcelain furnace is recommended. Finally, using diamond disc to open embrasure can lead cracks.

국가전략소재로서의 희유금속

이수영,지율권,최한신,김용환,이진규,김택수,Lee, Soo-Young,Ji, Yul-Kwon,Choi, Han-Shin,Kim, Hwan-Yong,Lee, Jin-Kyu,Kim, Taek-Soo 한국분말야금학회 2009 한국분말재료학회지 (KPMI) Vol.16 No.3

Expression of an artificial gene encoding a repeated tripeptide lysyl-g1utamyl-tryptophan in Tobacco Plant

이수영,나경수,백형석,박희성,조훈식,이용세,최장원,Lee, Soo-Young,Ra, Kyung-Soo,Baik, Hyung-Suk,Park, Hee-Sung,Cho, Hoon-Sik,Lee, Young-Se,Choi, Jang-Won Korean Society of Life Science 2002 생명과학회지 Vol.12 No.1

식물 단백질의 영양가 향상을 위한 일환으로 필수아미노산의 조성이 풍부한 인공단백질을 암호화하는 인공유전자를 담배 식물체에서 발현을 시도하기 위하여, 식물에서 외래유전자의 발현에 널리 사용되는 Cauliflower mosaic virus (CaMV)의 35S promoter를 이중으로 중첩되도록 하고, (Lys-Glu-Trp)이 64번 반복되는 인공유전자 및 nopaline synthase (nos) terminator를 갖고있는 binary vector pART4-4를 구성하였다. 이 재조합 플라스미드는 Agrobacterium tumefaciens를 이용한 형질전환에 의해 Nicotiann tabacum (Var. Xanthi)으로 도입되었다. Kanamycin이 포함된 신초 유도 배지 및 뿌리 유도배지를 이용하여 정상적으로 재생된 담배 식물체로부터 도입된 인공유전자의 발현을 분석하였다. 추출한 genomic DNA를 EcoRI으로 자른 다음 Southern blot 분석에 의하면, 효소 절단 시 예상되는 1.1 kb에서 band를 형성하였으며 각각의 형질전환 식물체에 인공유전자가 1 또는 3 개씩 도입되어 있음을 확인하였다. Northern blot 분석에 의하면 약 1.2 kb 전사체가 비교적 안정하게 발현되었으며, 잎, 줄기, 뿌리로부터 RNA를 분리하여 promoter의 조직 특이성 발현을 분석한 결과, 잎에서 생성되는 RNA가 줄기나 뿌리 조직보다 안정하게 발현되었다. 형질전환 식물체에서 Western blot에 의한 단백질 분석 결과, 잎에서 추출한 단백질로부터 원하는 크기인 33 kDa의 인공단백질이 생성됨을 확인하였으며 발현 수준은 전체 세포 단백질의 0.1%로서 낮은 수준이었다. To investigate expression of the artificial gene encoding a repeated tripeptide lysyl-glutamyl-tryptophan in tobacco plant, the plant binary vector, pART404 has been constructed, which contains the duplicated CaMV 35S promoter, an artificial gene coding for repetitive polymer (Lys-Glu-Trp)$_{64}$, and nopaline synthase (nos) terminator. The recombinant expression vector was introduced in Nicotiana tabacum (var. Xanthi) via Agrobacterium tumefaciens-mediated trans-formation. The transgenic calli selected by kanamycin containing medium were then regenerated to whole plants. Southern blot analysis indicated that five transgenic plants (No. 1, 7, 9, 43, 45) showed the hybridizing signals at 1.1 kb of the expected size on EcoRI digestion and each of the transgenic plants contained 1 or 3 copies of the artificial gene inserted into its genome. By northern blot analysis, the size of the hybridized total RNA was estimated to be approximately 1.2 kb and the RNA appeared generally to have the integrity. Western blot indicated that the protein was detected at the position of 33 kDa and the expression level of the polypeptide in the transgenic plant (No. 45) was measured to approximately 0.1% of the total protein.