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내인성 N1- methylnicotinamide 를 이용한 신혈장유량측정
심창구 한국약제학회 1983 Journal of Pharmaceutical Investigation Vol.13 No.1
Renal plasma flow (RPF) was estimated from the extraction ratio (E.R.) and renal plasma clearance of the endogenous N¹-methylnicotinamide(NMN) in the experimental renal failure (ERF) rat, and was compared with that of healthy rats. PRE of ERF-rats was not different from that of the healthy rat significantly and dependened upon E. R, or intrinsic clearance of the kidney. This NMN method seemed to be superior to the conventional p-aminohippuric acid (PAH) method clinically.
심창구(Chang-Koo Shim),김진웅(Jinwoong Kim) 大韓藥學會 2015 약학회지 Vol.59 No.2
The spring convention of the Pharmaceutical Society of Korea (PSK), composed of eight symposia and two special symposia, was held at the K Seoul Hotel on April 17th and 18th in 2014. During the convention, the board meeting of PSK officially approved the birth of Pharmacy History (PH) Division as one of 19 divisions of PSK on April 17th. In the morning of April 18th, an inaugural symposium of PH division was held at the hotel with a theme of "History of Pharmacy in Korea”. In this article, the establishment of PH division, content of the 1st symposium will be described. The future vision of PH division, which will be focused on the study of modern history rather than that of old history, will be described as well. The introduction of modern pharmacy education system to Korea and various events that happened during the establishment of the system may represent issues that should be investigated with the highest priority by the PH division.
제조 방법에 따른 간 모세담관막 소포계의 수송 특성 비교
심창구,정석재,송임숙 한국약제학회 1999 Journal of Pharmaceutical Investigation Vol.29 No.1
Canalicular liver plasma membrane vesicles (cLPM) were prepared according to two different methods (Inoue method and Meier method), and were evaluated for their protein yield, enzyme activity and transport characteristics. No difference was found between the methods in the protein yield (i.e., 0.14±0.031 and 0.15±0.050 ㎎/g liver for Inoue method and Meier method, respectively). The activity of alkaline phosphatase, a marker enzyme of canalicular membrane, was significantly (P<0.05) higher in the vesicles of Meier method (3.52±0.91 mmol/㎎/hr) than in the vesicles of Inoue method (2.28±0.94 mmol/㎎/hr) indicating that more purified cLPM were obtained from Meier method compared with Inoue method. ATP-dependent vesicular uptake of taurocholate and tributylmethylammonium (TBuMA) was observed for vesicles of both methods, and the kinetic parameters responsible for the transport were similar between the vesicles of both methods (for example, V_(max): 9.72 nmol/㎎ protein/30sec and K_m: 0.63 mM for Inoue method; V_(max): 10.1 nmol/㎎ protein/30sec and K_m: 0.70 mM for Meier method). A pH gradient dependent counter transport of TBuMA was also observed for both vesicles with similar kinetic characteristics. Either the uptake of taurocholate in the absence of ATP or that of TBuMA in the absence of pH gradient, which may represent passive diffusion of respective compound into the vesicles, was more rapid for the vesicles of Meier method than for the vesicles of Inoue method. For example, passive diffusion rate constants (K_d) for TBuMA uptake into the vesicles were 0.00030 and 0.00052 ㎕/㎎ protein/min for the vesicles of Inoue method and Meier method, respectively. It may indicate that more leaky vesicles are obtained form the Meier method compared with the Inoue method. These aspects together with the time necessary to prepare the vesicles (i.e., 8 hr for Inoue method and 23 hr for Meier method) should be considered before selecting an appropriate method for the preparation of cLPM.