고등식물로부터 Chitin Synthase Ⅱ 활성 저해물질의 탐색

황의일(Eui Il Hwang),이향복(Hyang Bok Lee),김성욱(Sung Uk Kim) 대한약학회 1999 약학회지 Vol.43 No.4

Chitin is an important structural component of fungal cell wall and is synthesized by chitin synthase Ⅰ, Ⅱ, and Ⅲ. The chitin synthase Ⅱ is an essential enzyme for the formation of primary septum in Saccharomyces cereviside. Therefore, specific inhibitors of this enzyme might block the formation of fungal cell wall and could be used as effective antifungal agents. To search chitin synthase Ⅱ inhibitors from natural products, 67 plants were extracted with methanol and examined for the inhibitory activities against chitin synthase Ⅱ of S. cerevisiae by our cell free assay system. As a result, the extracts from 16 plants showed more than 70% inhibition at the concentration of 280mcg/ml. Of note, Laurus nobilis (81.4%), Lonicera maackii (81.5%), Berchemia berchemiaefolia (82.9%), Koelreuteria paniculata (87.9%), Chamaecyparis pisifera (86%) and Taxus cuspidata (83.9%) inhibited strongly the chitin synthase Ⅱ activity.

오미자에서 분리한 새로운 Chitin Synthase Ⅱ 저해제인 Gomisin N 과 Wuweizisu C

황의일(Eui Il Hwang),김미경(Mi Kyung Kim),이향복(Hyang Bok Lee),김영국(Young Kook Kim),권병목(Byoung Mog Kwon),배기환(Ki Hwan Bae),김성욱(Sung Uk Kim) 대한약학회 1999 약학회지 Vol.43 No.4

To search for new chitin biosynthesis inhibitors from natural sources, several higher plants were examined the inhibitory activity against chitin synthase Ⅱ by enzymatic assay. Among them, the extract of Schizandra chinensis strongly showed the inhibitory activity against chitin synthase Ⅱ. Gomisin N and wuweizisu C were isolated from Schizandra chinensis and showed IC50 value of 62.4mcg/ml and 19.2mcg/ml, respectively. Activities of these compounds were more stronger than that of polyoxin D. However, gomisin N and wuweizisu C showed weakly antifungal activities against various human pathogens.

필터 생분해성 증진을 위한 종자 필터 개발 및 적용 효과

김수호,김민규,황의일,한영림,이창국,여운형,Kim, Soo-Ho,Kim, Min-Kyu,Hwang, Eui-Il,Han, Young-Rim,Lee, Chang-Kuk,Yeo, Woon-Hyung 한국연초학회 2014 한국연초학회지 Vol.36 No.1

Cigarette filters containing Brassica Rapa L. seeds of the dark brown and round shaped were evaluated to determine the effect of seed addition on filter degradability. The seeds with germination capability under the tar/nicotine condition in the preliminary test, were put into the active carbon part of the filter(12mm) during filter rod making by the kit. The $4{\pm}2$ pieces of the seeds were put into the opened fiber bundle of the filter tow. In order to test the germination rate of the seeds, seed filters were placed either in a petri dish or test-pot in a conditioned area ($25^{\circ}C$, 70 % RH). The seed filters were placed outdoors exposed to natural conditions with the periodic water supply. The seeds in the smoked filters showed 90 % germination rate after a month under the open air condition. No significant differences in the sensory evaluation and analysis were obtained when the control sample was compared to the same cigarettes with the seeds.

홍삼 생약 복합물(KTNG0345)이 섬유아세포의 Procollagen 생합성과 Matrix metalloproteinase-1(MMP-1) 활성에 미치는 영향

소승호,이성계,황의일,구본석,한경호,김나미,So, Seung-Ho,Lee, Seong-Kye,Hwang, Eui-Il,Koo, Bon-Suk,Han, Gyeong-Ho,Kim, Na-Mi 고려인삼학회 2007 Journal of Ginseng Research Vol.31 No.4

본 실험은 홍삼, 사상자, 산수유를 이용하여 주름 예방 효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 개별추출물과 이들의 혼합물 WM1, WM3 (KTNG0345)에 대하여 사람의 섬유아세포를 이용한 세포증식, 콜라겐 생합성 정도, MMP-1 효소활성, 항산화 활성을 조사하였다. 사용된 시료는 $1{\sim}10\;{\mu}g/ml$의 농도로 첨가하여 세포를 배양했을 때 $83{\sim}100%$의 세포증식율을 나타내어 세포 독성을 나타내지 않았으며, 콜라겐 생합성 정도는 대조군이 474.8 ng/ml이었고 retinoic acid는 568.3 ng/ml이었으며, 5 ug/ml의 농도를 기준으로 할 때 홍삼은 533.9ng/ml, 사상자는 539.3 ng/ml, 산수유는 514.1 ng/ml이었다. 홍삼과 생약을 68:232의 비율로 혼합한 WM1은 474.6 ng/ml이었고 홍삼과 생약을 136:164의 비율로 혼합한 WM3는 561.4 ng/ml로 증가하였다. MMP-1활성은 10 ug/ml의 농도에서 홍삼(ER)은 31.9 ng/ml, 사상자(WT)는 32.85 ng/ml, 산수유(WC)는 32.0 ng/ml, WM1은 31.3 ng/ml이었고 WM3(KTNG0345)는 28.85 ng/ml으로 대조군의 88% 수준으로 감소하였다. SOD활성에서는 1000 ug/ml의 농도로 처리하였을 때 65-97% 항산화활성을 나타내었다. 이들의 결과를 종합해 보면 홍삼과 사상자, 산수유 및 이들 혼합물은 human dermal fibroblas에 대하여 세포 독성을 나타내지 않으면서 콜라겐 생합성을 촉진하고 MMP-1의 활성을 억제하였으며 홍삼과 생약 엑기스를 136:164의 비율로 혼합했을 때 그 효과가 증진되었다. WM3 (KTNG0345)는 지표성분으로서 ginsenoside-$Rb_1$, torilin, loganin을 각각 10.85 mg/g, 0.128 mg/g, 3.92 mg/g 함유하였다. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-$Rb_1$, torilin and loganin were identified at 10.85 mg/g, 0.128 mg/g and 3.92 mg/g respectively.

증예(症例) : 좌 상행대정맥이 없는 우 상행대정맥의 결손

한영민 ( Young Min Han ),황의일 ( Eui Il Hwang ),진공용 ( Kong Yong Jin ) 전북대학교 의과학연구소 2001 全北醫大論文集 Vol.25 No.2

Absence of the right superior vena cava is a rare vascular anomaly. It is associated with a persistent left superior vena cava. We report a case of absence of right superior vena cava and zygous continuation without left superior vena cava features on CT scans.

천연소재 담배 필터 적용 방법

김민규,여운형,김수호,오경환,진용숙,황의일,Kim, Min-Kyu,Yeo, Woon-Hyung,Kim, Soo-Ho,Oh, Kyung-Hwan,Jin, Yong-Sook,Hwang, Eui-Il 한국연초학회 2013 한국연초학회지 Vol.35 No.1

The natural ingredient has been utilized variety of the food and medicine. The aromatherapy well known prevent disease and healthy promotion using the essential oil derived extracted plants. In this work, natural ingredient include herb granulated for application of tobacco filter. That used by granulation using fluidized bed granulator(top-spray, bottom-spray and tangential-spray) and wet granule method. According to ingredient can used granulating method selectivity. So, we used fluid-bed granulator and wet granule method. Grapefruit extract coated sugar particle using the bottom-spray method and red ginseng granule granulated red ginseng powder using the tangential-spray method in a fluidized bed. Then, these granules applied the tobacco filter after due consideration add amount and operation efficiency. As a result, wet granule was loaded dual filter because that similarity carbon granule. And it was fitted in added 3mg/mm, per tip in the tobacco end part. Another type, fuidized bed granules was filled cavity filter because it has high hardness, sphere shape.

홍삼 생약 복합물(KTNG0345)이 섬유아세포의 Procollagen 생합성과 Matrix metalloproteinase-1(MMP-1) 활성에 미치는 영향

소승호(Seung-Ho So),이성계(Seong-Kye Lee),황의일(Eui-Il Hwang),구본석(Bon-Suk Koo),한경호(Gyeong-Ho Han),김나미(Na-Mi Kim) 고려인삼학회 2007 Journal of Ginseng Research Vol.31 No.3

본 실험은 홍삼, 사상자, 산수유를 이용하여 주름 예방 효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 개별추출물과 이들의 혼합물 WM1, WM3(KTNG0345)에 대하여 사람의 섬유아세포를 이용한 세포증식, 콜라겐 생합성 정도, MMP-1 효소활성, 항산화 활성을 조사하였다. 사용된 시료는 1~10 ㎍/ml의 농도로 첨가하여 세포를 배양했을 때 83~100%의 세포증식율을 나타내어 세포 독성을 나타내지 않았으며, 콜라겐 생합성 정도는 대조군이 474.8 ng/ml이었고 retinoic acid는 568.3 ng/ml이었으며, 5 ㎍/ml의 농도를 기준으로 할 때 홍삼은 533.9 ng/ml, 사상자는 539.3 ng/ml, 산수유는 514.1 ng/ml이었다. 홍삼과 생약을 68 : 232의 비율로 혼합한 WM1은 474.6 ng/ml이었고 홍삼과 생약을 136 : 164의 비율로 혼합한 WM3는 561.4 ng/ml로 증가하였다. MMP-1활성은 10 ㎍/ml의 농도에서 홍삼(ER)은 31.9 ng/ml, 사상자(WT)는 32.85 ng/ml, 산수유(WC)는 32.0 ng/ml, WM1은 31.3 ng/ml이었고 WM3(KTNG0345)는 28.85 ng/ml으로 대조군의 88% 수준으로 감소하였다. SOD활성에서는 1000 ㎍/ml의 농도로 처리하였을 때 65-97% 항산화활성을 나타내었다. 이들의 결과를 종합해 보면 홍삼과 사상자, 산수유 및 이들 혼합물은 human dermal fibroblast에 대하여 세포 독성을 나타내지 않으면서 콜라겐 생합성을 촉진하고 MMP-1의 활성을 억제하였으며 홍삼과 생약 엑기스를 136 : 164의 비율로 혼합했을 때 그 효과가 증진되었다. WM3(KTNG0345)는 지표성분으로서 ginsenoside-Rb1, torilin, loganin을 각각 10.85 ㎎/g, 0.128 ㎎/g, 3.92 ㎎/g 함유하였다. Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-Rb₁, torilin and loganin were identified at 10.85 ㎎/g, 0.128 ㎎/g and 3.92 ㎎/g respectively.

증예(症例) : Vena Tech-LGM 필터의 자가적인 지주 골절 및 이동: 증례보고

한영민 ( Young Min Han ),이영환 ( Young Hwan Lee ),황의일 ( Eui Il Hwang ) 전북대학교 의과학연구소 2001 全北醫大論文集 Vol.25 No.1

The fracture of inferior vena cava filters is rare. We experienced a case of spontaneous fracture and migration of a Vena Tech LGM filter 8 months after placement in a 56-year-old man. Under such circumstances, we should be careful to perform other interventional procedure by fluoroscopic guidance and advisable to use another IVC filter procedure. The use of Vena-Tech-LGM filter in the placement of prevention of pulmonary thromboembolism in deep vein thrombosis patients should be cautious and close follow-up examination, and probably warning of strut fracture and migration of IVC filter.

홍삼 생약 복합물(KTNG0345)의 피부 주름개선에 관한 작용기전

소승호(Seung-Ho So),이성계(Seong-Kye Lee),황의일(Eui-Il Hwang),구본석(Bon-Suk Koo),한경호(Gyeong-Ho Han),정진호(Jin Ho Chung),이민정(Min-Jung Lee),김나미(Na-Mi Kim) 고려인삼학회 2008 Journal of Ginseng Research Vol.32 No.1

본 실험은 홍삼 혼합물 (KTNG0345)을 이용한 주름 예방 및 개선효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 시료를 경구투여한 무모생쥐의 피부조직으로부터 MMP-3의 발현양상과 작용 메커니즘을 연구하였다. MMP-3의 발현정도는 농도 의존적으로 현저한 감소를 나타내었으며, 유전자와 단백질 모두에서 동일한 양상을 보였다. PAK는 변화가 없었지만, p38, p-p38 그리고 c-Jun, p-c-Jun을 통계적으로 유의하게 감소시킴으로써 MMPs의 발현 감소를 가져온 것으로 보인다. 뿐만 아니라 자외선에 의한 TNF-α의 생성 또는 유입을 억제함으로써 TNF-α receptor에 의해 매개되는 신호전달 경로를 둔화시켜 MMPs의 발현을 감소시킨 것으로 보인다. 이렇게 KTNG0345는 복합적인 활성으로 작용하여 주름생성 억제 활성을 보이는 것으로 판단된다. UV irradiation causes skin-aging involving coarse wrinkles, thickening, dyspigmentation, and rough skin surface. These phenomena in complex skin tissue is controlled with receptor of cell surface growth factor and cytokine receptors. The activation of receptors induces multiple downstream signaling pathways including expression of MMPs (matrix metalloproteinases). This study was aimed to elucidate the mechanism for anti-wrinkle activity of Korean red ginseng, Torilis fructus and Corni fructus mixture (KTNG0345). In this animal study, we have investigated decreasing effects of Korean red ginseng mixture on MMP-3 synthesis through diminishing TNF-α signaling that express MMP-1, -3, and -9. c-Jun and c-fos as a component of transcription factor AP-1 (activator protein-1) were analyzed the expression level using real time PCR and western blotting. c-Jun was decreased dose dependent manner both gene and protein level where as cfos was not changed. In upstream, JNK and PAK was not changed, but p38 was decreased in down stream. MMP-3, final product in this pathway was significantly decreased in dose dependent manner. These results suggest that Korean red ginseng mixture have a anti-wrinkle activity through TNF-α mediated MMPs expression pathway.

하지 심부정맥혈전증에서의 스텐트의 사용 경험

이대영,한영민,이재백,유희철,황의일 대한혈관외과학회 2001 Vascular Specialist International Vol.17 No.2

Purpose: To evaluate the efficacy of venous stenting in the treatment of venous obstruction or stenosis of symptomatic deep vein thrombosis (DVT). Method: Eight consecutive patients with lower extremity DVT underwent venous stenting. The duration of symptoms were 20 days or less in 6 cases (acute) and above than 20 days in 2 cases (chronic). The cause of DVT was venous thrombophlebitis in 7 cases and extrinsic compression in 1 case. The indication of venous stenting is venous obstruction or stenosis after aspiration thrombectomy and direct catheter thrombolysis. The exclusion criteria were successful treatment of DVT with aspiration thrombectomy, direct catheter throtnbolysis and percutaneous angioplasty. The follow-up examination was performed 1 week, 2∼3 month interval by Doppler ultrasound, which was evaluated thrombi formation and patency of venous stenting. If the lesion detect in Doppler, second intervention was performed. Result: The venous stenting was successfully performed in all cases. Initial aspiratian thrombectomy was performed in all cases. The urokinase was performed in 4 cases which was 500,000∼750,000 IU (mean, 675,000) during from 2 to 8 hours (mean, 4). After that, the venous stenting was performed at the site of the venous occlusion and stenosis. The direct venography after stenting showed good reconstruction of vein and adequate restoration of venous flow. Seven patients relieved from lower extremity swelling without recurrence. One patient had re-thrombosis after venous stenting, which was successfully treated with percutaneous angioplasty. The follow-up duration was from 3 to 14 months (mean, 8 months). There were no thrombi in follow-up Doppler and normal regular life was resumed without lower extremity swelling. Conclusion: Venous stenting in the treatment of deep vein thrombosis is an effective and safe treatment method in the venous obstructive or stenotic remnant lesion despite following aspiration thrombectomy, direct catheter thrombolysis and percutaneous angioplasty.