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Overexpression of Zinc Finger Protein of Capsicum annuum (PIF1) in Tobacco Enhances Cold Tolerance
황을원,박수철,변명옥,최미영,권혁빈 한국유전학회 2008 Genes & Genomics Vol.30 No.2
Plants encounter various stresses from their surrounding environment, such as low temperature, drought and high salinity. Thus the plant responds to abiotic stress such as cold and drought through a range of different strategies including physiological and biochemical processes that are usually mediated by up-regulation of the expression of stress regulated genes. Zinc finger proteins play a role in abiotic stress tolerance such as cold, dehydration and salt. As one way to approach to cold defense mechanism in plants, we previously identified a gene for zinc finger protein (PIF1) from hot pepper ( Capsicum annuum) as cold inducible using cDNA microarray analysis coupled with Northern blot analysis. We previously showed that the PIF1 gene was induced strongly by cold stress, slightly by ABA. The PIF1 gene was engineered under control of CaMV 35S promoter for constitutive expression in transgenic tobacco plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting PIF1 transgenic tobacco plants showed significantly increased cold stress resistance. In addition, all PIF1 transgenic plants showed no visible phenotypic alteration compared to wild type plants. These results suggest the biological role of PIF1 in plant stress tolerances. Plants encounter various stresses from their surrounding environment, such as low temperature, drought and high salinity. Thus the plant responds to abiotic stress such as cold and drought through a range of different strategies including physiological and biochemical processes that are usually mediated by up-regulation of the expression of stress regulated genes. Zinc finger proteins play a role in abiotic stress tolerance such as cold, dehydration and salt. As one way to approach to cold defense mechanism in plants, we previously identified a gene for zinc finger protein (PIF1) from hot pepper ( Capsicum annuum) as cold inducible using cDNA microarray analysis coupled with Northern blot analysis. We previously showed that the PIF1 gene was induced strongly by cold stress, slightly by ABA. The PIF1 gene was engineered under control of CaMV 35S promoter for constitutive expression in transgenic tobacco plants by Ti-plasmid of Agrobacterium-mediated transformation. The resulting PIF1 transgenic tobacco plants showed significantly increased cold stress resistance. In addition, all PIF1 transgenic plants showed no visible phenotypic alteration compared to wild type plants. These results suggest the biological role of PIF1 in plant stress tolerances.
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유병국,이재희,신선주,황을원,권혁빈 한국식물학회 2013 Journal of Plant Biology Vol.56 No.2
EREBP (ethylene responsive element binding protein) and WRKY (wizz-like transcription factor) are known to play roles in plant tolerance to abiotic stresses, such as low temperatures. Previously, we used cDNA microarrays and Northern blot analysis to determine the mechanisms responsible for those underlying defenses. These analyses led to the identification of CaEREBP-C1, -C2, -C3 and CaWRKY1A genes that encode the ethylene responsive element binding protein and wizz-like transcription factors, respectively, from hot pepper (Capsicum annuum). In that study, we demonstrated that the CaEREBP-C1, -C2, -C3 and CaWRKY1A genes were strongly induced by cold stress. Here, we used Tiplasmid and Agrobecterium-mediated transformation to engineer CaEREBP-C1, -C2, -C3 and CaWRKY1A under control of the CaMV 35S promoter for constitutive expression in transgenic plants. The resultant transgenic plants exhibited significantly increased tolerance to low temperatures. In addition, none of the CaEREBP-C1, -C2, -C3 and CaWRKY1A transgenic plants showed visible phenotypic alteration when compared to wild type plants. Taken together, these results suggest that CaEREBP-C1, -C2, -C3 and CaWRKY1A play important biological roles in conferring plant cold stress tolerance.
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