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Confocal Microscopy Study of Arabidopsis Embryogenesis Using GFP:mTn
황성수 한국식물학회 2009 Journal of Plant Biology Vol.52 No.4
Embryogenesis in transgenic Arabidopsis plants with GFP:mTn, a chimeric fusion of soluble shifted green fluorescent protein and a mouse actin binding domain, was studied. Confocal laser scanning microscopy was used to determine patterns of formation and cellular responses during asymmetric cell division. Before such cells divide, the nucleus moves to the position where new cell walls are to be formed. The apical–basal axis of the embryo develops mainly at the zygote to octant stage, and these events are associated with asymmetric divisions of the zygote and hypophyseal cells. Formation of the radial axis is established from the dermatogen to the globular-stage embryo via tangential cell division within the upper tiers. Bilateral symmetry of the embryo primarily happens at the triangular stage through zig-zag cell divisions of initials of the cotyledonary primordia. All stages of embryogenesis are described in detail here.
황성수,조은숙,고지영,장은정,주재욱,손정일,심희정,이종현,Hwang, Seong-Soo,Cho, Eun-Sook,Ko, Ji-Young,Jang, Eun-Jung,Joo, Jae-Uk,Son, Jung-Il,Sim, Hee-Jung,Lee, Jong-Hyeon 대한고유수용성신경근촉진법학회 2011 PNF and Movement Vol.9 No.1
Background : This study was to investigate effect of hot and cold water for the hand dexterity. Methods : A total of 37 subjects having hemiplegia(men=6, women=11) and normal(men=10, women=10) were participated in this study. After both hands was soaked in the small tube for 1 minute, we measured 'making dots in circles(BOTMP)', 'lifting small objects(JHFT)', 'disk turning(MMDT)' test. Results : In the case of normal's dominant hand, there were statistically significant differences in all tests after soaked in the hot water but there were statistically significant differences in 'disk turning' test after soaked in the cold water. In the case of hemiplegia's unaffected hand, there were statistically significant differences in 'disk turning' test after soaked in the hot water but there were statistically significant differences in 'making dots in circles', 'disk turning' test after soaked in the cold water. In the case of hemiplegia's affected hand, there were statistically significant differences in 'lifting small objects', 'disk turning' test after soaked in the hot water but there were statistically significant differences in 'making dots in circles', 'disk turning' test after soaked in the cold water. Conclusion : The hot and cold water were affected in the hand dexterity. Especially, in the case of both normal and hemiplegia, there were statistically significant differences in 'disk turning' test after soaked in the cold and hot water. Therefore, We were founded that affected in gross movement than fine movement.