Global Analysis of Estrogen-Regulated Genes in Mouse Uterus using cDNA Microarray and Laser Capture Microdissection

홍석호,나희영,이지윤,김정훈,김문규,Hong, Seok-Ho,Nah, Hee-Young,Lee, Ji-Yoon,Kim, Chung-Hoon,Kim, Moon-Kyoo The Korean Society for Reproductive Medicine 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.2

연구목적: Estrogen은 포유류의 생리주기와 착상과정에서 중요한 조절인자로 작용한다. 본 연구에서는 난소 절제된 생쥐의 자궁에서 estrogen에 의해 직접 또는 간접적으로 조절되어 발현하는 유전자를 분석하고자 하였다. 연구재료 및 방법: 생후 8주된 생쥐의 양쪽 난소를 절제하고 14일 동안 회복기간이 지난 후, estrogen (300 ng/mouse)을 피하로 주사하였다. Estrogen 주사 후 6, 12시간째 자궁을 적출하여 cDNA microarray와 laser capture microdissection (LCM) 기술을 이용하여 estrogen에 의해 조절되는 유전자의 시공간적인 발현 양상을 조사하였다. 결 과: Estrogen 주사 후 6시간째에는 조사된 전체 유전자 가운데 0.9% (증가 22, 감소 49), 12시간째에는 8.4% (증가 351, 감소 287)에 해당되는 유전자가 두 배 이상 증가 혹은 감소하는 결과를 보였다. 또한 일부 증감된 유전자를 선택한 후 LCM 기술을 이용하여 시공간적인 발현양상을 확인한 결과 자궁내막상피세포에서만 estrogen에 의해 유전자의 발현이 증가되는 일부 유전자를 선별하였다. 결 론: 이상의 결과들을 종합해보면 1) estrogen에 의해 조절되는 유전자의 수나 증감의 정도는 12시간 이후에 더 많고, 크게 조절되며, 2) 유전자의 조절부위가 자궁의 특이적인 세포층에서 시공간적으로 조절됨을 의미한다. 이러한 유전자의 정보는 생리주기 또는 착상과정의 분자생물학적 기작을 이해하는 데 도움이 될 것이다.

수술 후 재발한 라스케열 낭 - 증례보고 -

홍석호,곽호신,정희원,Hong, Seokho,Gwak, Ho-Shin,Jung, Hee-Won 대한신경외과학회 2000 Journal of Korean neurosurgical society Vol.29 No.2

A case of symptomatic Rathke's cleft cyst, which recurred five months after drainage of the cyst using transs-phenoidal approach, is presented. Symptomatic recurrence after surgery of Rathke's cleft cyst is very rare and nine cases have been reported in the literatures. The radiological and pathologic features and clinical course of this unusual case are described. Recent concepts about embryological pathogenesis and clinical behavior mimicking craniopharyngioma are discussed with the review of literature.

생쥐 배아에서 Heparin-Binding Epidermal Growth Factor가 MMP-9과 ATPase ${\gamma}$-subunit 유전자의 발현에 미치는 영향

홍석호,나희영,이지윤,김정희,김정훈,채희동,강병문,김문규,Hong, Seok-Ho,Nah, Hee-Young,Lee, Ji-Yun,Kim, Jung-Hee,Kim, Chung-Hoon,Chae, Hee-Dong,Kang, Byung-Moon,Kim, Moon-Kyoo 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.2

Objectives: The purpose of this study was to evaluate effects of heparin-binding epidermal growth factor (HB-EGF) on the rate of blastocyst formation and hatching in the mouse embryos and the expression of matrix metalloproteinase-9 (MMP-9) and ATPase ${\gamma}$-subunit mRNA. Methods: Late 2-cell mouse embryos was cultured for 72 hours in RTF medium containing with 1, 10, and 100 ng/ml HB-EGF. The mRNA expression level of MMP-9 and ATPase ${\gamma}$-subunit was detected by reverse transcription-polymerase chain reaction (RT-PCR). Results: The rate of hatching was significantly higher (p<0.05) in group containing with 1 ng/ml HB-EGF than other groups. Also, the rate of hatched blastocyst was significantly higher (p<0.05) in 10 ng/ml. The mRNA expression level of MMP-9 mRNA was not shown any difference among groups, but ATPase ${\gamma}$-subunit was higher than other groups. Conclusions: Taken together these results suggest that HB-EGF has the positive effect to promote the blastocyst formation and hatching process and influences the blastocoel expansion by promoting the ATPase mRNA expression in the mouse embryos.

착상 전 생쥐배아에서 Id 유전자의 발현

홍석호,나희영,이영진,이지원,손영수,채희동,김성훈,강병문,김정훈,Hong, Seok-Ho,Nah, Hee-Young,Lee, Young-Jin,Lee, Ji-Won,Son, Young-Soo,Chae, Hee-Dong,Kim, Sung-Hoon,Kang, Byung-Moon,Kim, Chung-Hoon 대한생식의학회 2004 Clinical and Experimental Reproductive Medicine Vol.31 No.4

Objective: The Id family of helix-loop-helix proteins are thought to affect the balance between cell growth and differentiation by negatively regulating the function of basic-helix-loop-helix (bHLH) transcriptional factors. The aim of this study was to investigate the expression pattern of Ids (Id-1, -2, -3, and -4) in preimplantation mouse embryos at mRNA and protein levels. Methods: Oocytes and preimplantation embryos were collected from reproductive organs of female ICR mice following superovulation. RT-PCR was performed to investigate the mRNA expression patterns of Id genes and their protein were localized by immunofluorescence analysis. Results: Id-1 and Id-3 mRNAs were strongly expressed at the germinal vesicle (GV) oocyte and the blastocyst stages. Id-2 mRNA was expressed throughout preimplantation embryo development, but Id-4 was not expressed. Immunofluorescence showed that Id-1 and Id-2 were predominantly localized in cytoplasmic region, but the immunofluorescence signal of Id-3 was weak throughout preimplantation embryo development. Conclusion: These data show for the first time that Ids are expressed in preimplantation mouse embryos and suggest that Ids may play an important role in early preimplantation embryo development and uterine physiological changes.

중복이용구역 지정을 통한 입체도시계획기법에 관한 연구

홍석호(Hong, Suk-Ho),김세용(Kim, Sei-Yong) 대한건축학회 2013 대한건축학회논문집 Vol.29 No.12

Fast paced urbanization has raised property values and increased populations but provisions of infrastructure have not adequately followed demand. This issue has created a poor and inadequate city center environment. Resulting are ever rapidly increasing property values, scarcity of further infrastructure expansionandotherdifficultiesforrelatedindustries. The current reality is that transport planners do not have standardized methods to maintain a diversity of living space. Especially in light of the dynamic changes occurring in the global economy, the existing working methods are beginning to show their limitations. Japan passed the ‘Double Use Road’ regulation in 1989, later on the ‘Double Use Park’ law along with various other laws and regulations regarding the forms of urban space. The application of these regulations towards urban space showed a great degree of flexibility in regards to public and private partnership as these reforms were done in consultation with owner to ensure that the securing of infrastructure went smoothly. This study carried out an inquiry and analysis of the double use zoning designation in urban planning with Japan as its main case study. Relevant laws and regulations were systematically categorized. Additionally, the application of double use zoning was briefly analyzed in its application as a tool for revitalization, which henceforth will prove of vital importance in Korea.

폐측지선(閉測地線)의 존재(存在)에 관하여

홍석호 ( Suk Ho Hong ) 건국대학교 교육연구소 1983 교사와 교육(구 교육논집) Vol.7 No.-

In this note, we wish to study the existence of closed geodesics. Our purpose is to discuss the divisibility that is, to obtain a new conditions for a divisibility including the case of non-riemannian metrics.

한국 노년층의 사회자본에 대한 인식 유형화 및 영향요인

홍석호(Hong, Seokho),황선영(Hwang, Sunyoung),김순은(Kim, Soon Eun) 한국노년학회 2017 한국노년학 Vol.37 No.3

본 연구는 노인들의 개인수준에서 사회자본을 어떻게 인식하고 있는지에 대한 유형을 분류하고 이러한 차별화된 유형에 영향을 미치는 요인들을 실증적으로 분석하는 것을 목적으로 한다. 연구목적 달성을 위해 2014년 전국거주 만 65세 이상 1,060명 노인을 대상으로 조사한 ‘노인문제 및 지역사회자본 조사연구’ 데이터를 활용하여 K-평균 군집분석과 다항명목 로지스틱 회귀분석을 실시하였다. 본 연구의 주요변수인 사회자본은 신뢰,규범, 네트워크, 사회참여로 측정하였고 영향요인으로는 인구학적, 사회계층적, 정치종교적, 그리고 주거환경적 변수들을 선택하였다. 분석 결과 노인의 사회자본 인식에 대한 3가지 유형(사회자본 잠재형, 사회자본 단절형, 사회자본 풍족형)이 발견되었다. 3가지 유형들은 서로 다른 외재적 특징(나이, 교육, 생활수준, 주택소유,정치성향, 종교, 거주지역, 주거형태)을 가지고 있었다. 사회자본 잠재형과 비교하여 나이가 젊을수록, 정치 성 향이 진보적일수록, 종교가 없다고 응답할수록, 농어촌에 거주하지 않을수록 사회자본 단절형에 속할 확률이 높았다. 반면, 여성일수록, 학력이 높을수록, 연립과 다세대 주택에 거주하지 않을수록, 생활수준이 높을수록, 기독교를 믿을수록, 농어촌에 거주하지 않을수록 사회자본 풍족형에 속할 확률이 높게 나타났다. 본 연구의 분석결과를 기반으로 하여 실천적․정책적 제언을 제시하였다. Korea is proceeding to aging society at much faster pace than other countries. To alleviate various problems (health, environment, social relationships, psychology and emotion, and financial problems) of the elderly caused by aging society, there has been increasing attention to social capital. This study investigated types of ways that the elderly at individual level are aware of social capital and the characteristic. In addition, factors that influence the types were studied. Data from 1060 elderly with the age of 65 and older were collected in Korea. Excluding missing data on variables used in the study, data of 960 elderly were used for analyses. K-mean cluster analysis was conducted to investigate types of elderly awareness on social capital at individual level. Multinominal logistic regression was used to find out factors of the types. K-mean cluster analysis resulted in three types of awareness on social capital among the elderly: (1) potential of social capital; (2) severance of social capital; and (3) wealth of social capital. Multinominal logistic regression resulted in that compared to potential of social capital type, when the elderly were younger, inclination of politics was more progressive, they did not have religion, they did not reside in rural, and they did not live in multiplex housing, the elderly were more likely to be in severance of social capital type. Also, when the elderly were women, had higher education and standard of living, were Christian, and did not reside in rural regions. they tended to be in wealth of social capital type. Based on the study results about awareness on social capital among the elderly and factors related to the awareness types, practical and political suggestions that promote social capital to solve problems of the elderly were provided.

최근(最近) 일본위상수학(日本位相數學)의 연구동향(硏究動向)

홍석호 ( Suk Ho Hong ) 건국대학교 교육연구소 1978 교사와 교육(구 교육논집) Vol.3 No.-

이 글의 내용은 Pittsburgh 대학의 Nagata 교수가 最近 日本에 있어서 位相數學(General topology)의 硏究動向에 관한 강연요지이다. 一般化된 거리공간은 日本에서 가장 활발히 硏究되고 있는 한 분야이다. 이미 發表된 內容과 아직 출판되지 않은 훌륭한 結果들을 뭏아 問題解決의 순서에 따라 整理하였다.

Topological Entropy에 관한 연구(硏究)

홍석호 ( Suk Ho Hong ) 건국대학교 교육연구소 1982 교사와 교육(구 교육논집) Vol.6 No.-

Bowen [1]은 位相的 entropy를 거리공간의 一樣연속사상에 대하여 定義하고 Lie-群의 affine 사상에 대하여 계산하였다. 本 硏究에서는 compact 位相多樣體上의 일반적인 位相同型 사상의 位相的 entropy에 관해서 연구하였다.

Morse 복체에 관하여

홍석호 ( Suk Ho Hong ) 건국대학교 교육연구소 1986 교사와 교육(구 교육논집) Vol.10 No.-

本 論文은 Morse 복체를 정의하고 强한 의미의 非退化 條件 아래서 다음을 증명하였다. 1) Morse 복체는 C.W. 분할 가능하다. 2) H*(M)=H*(∧M) 3) 閉測地線이 雙曲型이면 index c(m)=m index c.