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EBV-Positive T/NK-Cell Lymphoproliferative Disease of Childhood
홍민의,고영혜,유건희,구홍회,김석진,김원석,박희정 대한병리학회 2013 Journal of Pathology and Translational Medicine Vol.47 No.2
Background: Epstein-Barr virus (EBV)-associated hemophagocytic lymphohistiocytosis (HLH), EBV-positive systemic T-cell lymphoproliferative disease (STLPD) of childhood, and chronic active EBV (CAEBV) infection may develop after primary EBV infection. This study reviewed the clinicopathological spectrum of EBV-associated T- and natural killer (NK)-cell LPD, including STLPD and CAEBV infection, with an analysis of T-cell clonality. Methods: Clinicopathological features of seven patients with EBV-associated HLH or STLPD and 12 patients with CAEBV infection were reviewed. Immunohistochemical staining and a T-cell receptor (TCR) gene rearrangement study were performed. Results: STLPD and EBV-positive HLH showed significantly overlapping clinicopathological findings. One patient with STLPD and one patient with EBV-positive HLH demonstrated moderate to severe atypia of the infiltrating lymphocytes, whereas the remaining patients lacked significant atypia. Twelve patients had CAEBV infection, four of whom suffered mosquito-bite hypersensitivity, five showed NK lymphocytosis, and one suffered hydroa vacciniforme. Infiltrating lymphocytes were predominantly small and devoid of atypia. Hemophagocytic histiocytosis was found in seven of 11 patients. Monoclonality was detected in three (50%) of the six patients with successful TCR gene analysis. Conclusions: EBV-positive HLH and STLPD share similar clinicopathological findings and may constitute a continuous spectrum of acute EBV-associated T- or NK-cell proliferative disorders. The distinction of EBV-positive T-cell LPD from EBV-positive HLH may be difficult during routine diagnoses because of the technical limitations of clonality assessment.
홍민의,김정원,신미경,김병천 대한의학회 2017 Journal of Korean medical science Vol.32 No.10
In colorectal carcinoma, poorly differentiated clusters (PDCs) are a poor prognostic indicator and show morphological continuity and behavioral similarities to micropapillary patterns (MPPs) as well as tumor buds (TBs). Epithelial-mesenchymal transition (EMT) and inhibition of cancer-stromal interactions may contribute to the development of PDCs. To clarify the biological nature of PDCs, we examined immunohistochemical stainings for β-catenin, Ki-67, E-cadherin, epithelial cell adhesion molecule (EpCAM), MUC1, and epithelial membrane antigen (EMA), which are associated with EMT and cancer-stromal interactions. The expression frequencies and patterns of PDCs, TBs, and differentiated neoplastic glands from the tumor center (TC) were compared. In the study group (117 cases), the nuclear β-catenin staining index was higher in PDCs (37.3%) and TBs (43.3%) than in neoplastic glands from TC (8.9%, P < 0.001). The mean Ki-67 labeling index in TC was 71.5%, whereas it was decreased in PDCs (31.2%) and TBs (10.2%, P < 0.001). E-cadherin and EpCAM displayed a tendency to be found along the cell membrane in TC samples (91.5% and 92.3%, respectively), whereas they showed loss of membranous staining in PDC (44.4% and 36.8%, respectively) and TB samples (60.7% and 68.4%, respectively). An inside-out pattern for MUC1 and EMA was frequently observed in PDC (48.7% and 45.3%, respectively) and TB samples (46.2% and 45.3%, respectively), but not in TC samples. Our data demonstrate that there is a pathogenetic overlap among PDCs, TBs, and MPPs and suggest that they might represent sequential growth patterns that branch from common biological processes such as dedifferentiation and alteration in cancer-stromal interactions.
홍민의,김한비,심상준 한국공업화학회 2015 한국공업화학회 연구논문 초록집 Vol.2015 No.0
Alzheimer’s disease (AD), which is the most common form of dementia,is characterized by a widespread functional disturbance of the human brain. Brain plaque deposition in the form of beta amyloid (Aβ) is a pathological hallmark of AD. The Aβ deposition is facilitated by an isoform of Apolipoprotein E4 (ApoE4). Here, we present a nanoplasmonic biosensor to detect the dynamics of ApoE4-mediated Aβ aggregation. This sensor is based on the localized surface plasmon resonance (LSPR) of a single gold nanoparticle. In this sensor, ApoE4 is exploited as an inducer of Aβ42 aggregation and the aggregation of Aβ42 is more specific and rapid than that of Aβ40. In addition, a detection limit of 10 μM for Aβ42 can be obtained corresponding to the 12.5 nm LSPR-peak shift, which is in line with the requirement for clinical detection. This is the first platform for the real-time detection of Aβ aggregation, mimicking the biological conditions, which can be used to investigate AD directly in the future.
Efficient bioconversion of CO<sub>2</sub> in microalgae using multiscale strategies
홍민의,장원석,심상준 한국공업화학회 2014 한국공업화학회 연구논문 초록집 Vol.2014 No.1
The multiscale strategies were systematically designed and operated for efficient capture and the bioconversion of CO<sub>2</sub> using microalgal biomass under photoautotrophic condition. In microscale strategy, the relationship between phototaxis and photosynthesis was revealed using microfluidic device. In bench scale strategy, an autotrophic astaxanthin production was improved by highly photosensitive mutant and Habber-Weiss Reaction (Superoxide-Driven Fenton Reaction) under high temperatures in Haematococcus cells. In pilot scale strategy, a closed-photobioreactor with a vertical bubble column type easy to scale-up and efficient for capture of CO<sub>2</sub> was developed. In our work, particularly, an industrial flue gas and sunlight were used for an economical microalgal culture system. The microscale approach fit for strain improvement insures the simple strategy for isolation of highly efficient strains in photosynthesis. The benchscale approach is suitable for process development and will be useful for application to pilot scale. These two approaches will synergically improve the pilotscale approach which is crucial for commercialization of the autotrophic microalgal system.
홍민의,홍순억,권기영,이태진,박언섭,차성재,도재혁,유재형 대한병리학회 2011 Journal of Pathology and Translational Medicine Vol.45 No.2
A 75-year-old man was referred to our hospital with intestinal obstruction caused by intussusception. Abdominal computed tomography (CT) revealed seven polypoid masses in the small intestine, while chest CT revealed a mass in the right lower lobe. Preoperative laboratory tests showed white blood cell (WBC) and neutrophil differential counts of 63,630/mm³ and 95%, respectively. The serum granulocyte colony-stimulating factor (G-CSF) was 114 pg/mL, which was elevated (normal range, <18.1 pg/mL). After resection of the small bowel, the WBC count decreased to 20,510/mm³. The pathology showed a poorly differentiated carcinoma with sarcomatous components confirmed by positive immunostaining of cytokeratin (AE1/AE3) and vimentin in the small intestine. Furthermore, immunohistochemistry with specific monoclonal antibodies against G-CSF was positive. A lung biopsy revealed the same histological findings as the small intestine lesion. Therefore, the patient was diagnosed as having a G-CSF producing sarcomatoid carcinoma of the lung with metastasis to the small intestine
홍민의,고영혜 대한병리학회 2015 Journal of Pathology and Translational Medicine Vol.49 No.6
Eosinophilic ulcer of the oral mucosa (EUOM) is a very rare, benign, self-limiting ulcerative lesion of the oral cavity of unknown pathogenesis, and belongs to the same spectrum of CD30+ T-cell lymphoproliferative disease (LPD) of the oral mucosa. The etiology and pathogenesis of the disease are unknown. We report two cases in children who were initially diagnosed with EUOM and CD30+ T-cell LPD, respectively. However, retrospective analysis revealed that a majority of infiltrated atypical T cells were positive for Epstein-Barr virus (EBV). The present cases suggest that the pathogenesis and etiology of EUOM or CD30+ T-cell LPD occurring in children are different from those in adults. EUOM or CD30+ T-cell LPD in children is a manifestation of EBV-positive T-cell LPD, and should therefore be distinguished from the disease in adults.