人蔘 Ethanol 抽出物 投與가 白鼠脂肪組織의 遊離脂酸代謝에 미치는 影響 : 인삼 Ethanol 추출물 투여가 백서지방조직의 유리지산대사에 미치는 영향

홍기환 부산대학교 1974 論文集: 自然科學篇 Vol.17 No.-

白鼠 脂肪組織의 遊離脂酸代謝에 미치는 Prostaglandin E1의 作用 : 백서 지방조직의 유리지산대사에 미치는 Prostaglandin E1의 작용

홍기환 부산의대 1973 부산의대잡지 - 부산대학교 의과대학 Vol.13 No.2

子宮筋의 弛緩後 興奮作用機轉에 關하여 : Ⅰ. 家兎摘出子宮에 있어서 isoproterenol 誘發 後興奮

홍기환 부산의대 1976 부산의대잡지 - 부산대학교 의과대학 Vol.16 No.2

白鼠脂肪組織의 遊離脂酸代謝에 미치는 人蔘 saponin의 作用 : 백서지방조직의 유리지산대사에 미치는 인삼 saponin의 작용

홍기환 부산대학교 1973 論文集: 自然科學篇 Vol.16 No.-

가토심근(家兎心筋) Mitochondria 분획내(分劃內) Adenosine triphosphatase 활성도(活性度)에 대(對)한 Diphenylhydantoin sodium 및 Quinidine의 작용(作用)

홍기환,Hong, Ki-Whan 대한약리학회 1972 대한약리학잡지 Vol.8 No.1

The author studied the actions of ouabain and diphenylhydantoin sodium on the ATPase activity in mitochondrial fraction isolated from rabbit heart and compared with that of quinidine. The results obtained are as follows: 1) In studying the $(Na^++K^+)-activated$ ATPase activity, the rabbit heart isolated was immediately frozen for 7-9 days (ageing of preparation) and thereafter the mitochondria1 fraction obtained by differential centrifugation technic was treated with solution A containing 0.15% deoxycholate for 24-48 hours at $-10^{\circ}C$ before using in experiment. These methods increased the activity ratio to 0.87-0.98. 2) The $(Na^++K^+)-activated$ ATPase activity in mitochondrial fraction of rabbit heart was not completely but markedly inhibited by ouabain. This inhibitory action of ouabain was moderately antagonised by $K^+$ concentration at constant Na concentration. 3) Diphenylhydantoin sodium in concentration of $5{\times}10^{-4}{\sim}10^{-3}M$ stimulated markedly not only $Mg^{++}-dependent$ ATPase activity but also $(Na^++K^+)$-activated ATPase activity and in concentration lower than $10^{-6}M$ had little effect. However, this effect of diphenylhydantoin was markedly increased in the presence of $Na^+$ alone rather than $K^+$ alone, but lesser than that effect in the presence of both $Na^+$ and $K^+$, together. The stimulating effect of diphenylhydantoin was specifically antagonized by ouabaion. 4) When the rabbits were intravenously injected with ouabain and diphenylhydantion respectively, $(Na^++K^+)-activated$ ATPase activity of rabbit heart of ouabain-treated group was much decreased and both $(Na^++K^+)-activated$ ATPase and $Mg^{++}-activated$ ATPase activity were moderately increased in diphenylhydantoin-treated rabbit group. 5) The $(Na^++K^+)-activated$ ATPase activity in mitochondrial fraction of rabbit heart was slightly inhibited by quinidine in high concentration of $10^{-4}M$, but nearly little effect was observed below the concentration of $5{\times}10^{-5}M$. 6) It might be possible to conclude that diphenylhydantoin specifically antagonised the action of ouabain on the membrane ATPase, which is different from the action of quinidine.

설암 및 구강저암의 수술적 치료

홍기환,양윤수 대한기관식도과학회 1997 大韓氣管食道科學會誌 Vol.3 No.2

The records of 18 patients with squamous cell carcinoma of the tongue and floor of mouth treated surgically were reviewed. Surgical approaches, staging, treatment modalities, recurrence and vital status were evaluated. The distrubutions of involved sites were tongue(9 cases) and mouth floor(9 cases). Patients were treated by surgery primarily, combined chemotherapy and radiation, and by surgical salvage in the failure cases of radiation and chemotherapy. All ipsilateral necks of mouth floor cancer and advanced tongue cancer were treated with neck dissection. Cases of early tongue cancer could be excised with transoral route, and advanced cases needed transmandibular approach. Whereas, majorites of mouth floor cancer needed transmandibular approach, and other cases could be excised transoral and pull-through approaches. In the recontructions, we used primary closure, pectoralis major myocutaneous flap, forearm free flap, fibular osteocutaneous flap and skin graft. One year survival rate was 93% and 2 year survival rate was 60%.

가토(家兎) 및 백서자궁(白鼠子宮)의 Adrenotropic Receptors에 관(關)한 연구(硏究)

홍기환,Hong, Ki-Whan 대한약리학회 1966 대한약리학잡지 Vol.2 No.1

The author studied the adrenotropic receptors of the non-pregnant uteri of the rabbit and rat, using epinephrine (alpha and beta activator), phenoxybenzamine(alpha blocking agent) and nethalide (beta blockade), and obtained the following results: 1. The spontaneous motility of isolated non-pregnant uteri from rabbits were stimulated by epinephrine, whereas that of isolated non-pregnant rat uterus was inhibited by epinephrine. 2. Both alpha and beta adrenergic receptors were present in the uterine muscle of both animals. 3. In the non-pregnant rabbit uterus, alpha receptors were predominant, whereas in the non -pregnant rat uterus, beta receptors preponderated over alpha receptors.

갑상선 수술 후 증후군

홍기환 대한이비인후과학회 2014 대한이비인후과학회지 두경부외과학 Vol.57 No.5

Phonatory and swallowing symptoms after thyroidectomy are well-known implications of laryngeal nerve injuries. The laryngeal nerve injuries, superior and inferior, are major complication that results in changes both to voice quality and production of high-pitched sounds, which is of utmost importance for voice professionals. However, these functional and subjective voice and swallowing symptoms were often neglected by clinicians. Most voice and swallowing alterations after thyroidectomy are self limited and are not related to demonstrable impaired nerves function. Several causes have been supposed to interpret these symptoms. They have been attributed to arytenoid trauma after endotracheal intubation, surgical trauma, modification of the vascular supply of the larynx, local pain in the neck, cricothyroid dysfunction, strap muscle mal-function, laryngotracheal fixation with impairment of vertical movement, and psychologic reaction to the operation. Because of the impact of patient’s perception on postoperative outcome in recent years, some groups have extensively investigated this functional post-thyroidectomy syndrome. All the published studies demonstrate that most patients who undergo thyroidectomy have at least some subjective vocal and/or swallowing complaints early after the opera-tion. Despite increasing interest, prospective data about the long-term outcomes of functional postthyroidectomy syndrome are lacking. This paper is to review the long-term trend and evolution of voice and swallowing symptoms after thyroidectomy in the absence of laryngeal nerve injury. Korean J Otorhinolaryngol-Head Neck Surg 2014;57(5):297-303

뇌졸중증(腦卒中症)에 있어서 혈소판(血少板) 응집( 凝集)의 역할에 관한 연구(硏究)

홍기환,이원석 大韓藥理學會 1982 대한약리학잡지 Vol.18 No.2

한국(韓國)에서 빈발(頻發)하는 뇌졸중증(腦卒中症) 환자(患者)의 혈장(血漿)에서 혈전형성(血栓形成)에 관계되는 인자(因子)들을 조사하고, in vitro로 sodium niroprusside의 혈소판(血少板)응집에 대(對)한 작용(作用)을 관찰(觀察)하여 뇌졸중증(腦卒中症) 응급시(應急時) 치료적응용(治療的應用)의 가능성(可能性)을 검토(檢討)하였다. 1) 뇌졸중증(脚卒中症) 환자(患者)의 혈장내(血漿內) 미세혈전형성(微細血栓形成)은 건강인(健康人)에 비(比)하여 유의(有意)하게 (p<0.001) 높았다. 2) 뇌졸중증(腦卒中症) 환자(患者)의 혈장내(血漿內) 과산화지질(過酸化脂質) 및 cathepsin D치(値)는 건강인(健康人)에서 보다 현저히 증가되어 있었다. 3) 건강인(健康人)의 혈소판(血少板)은 1 및 <TEX>$2{\mu}M\;ADP$</TEX>에 대(對)하여 2차반응(次反應)에서는 응집률(凝集率)이 감소(減少)된데 반(反)하여 뇌졸중증환자(腦卒中症患者)에서는 응집률(凝集率)이 항진(亢進)되었다. 4) <TEX>$K^+-free\;PSS$</TEX>, ouatain, 13-HPLA 및 Cd으로 전처치(前處置)한 소 뇌저동맥(腦底動脈), 토끼 대동맥(大動脈) 및 사람 제대동맥(臍帶動脈)은 혈소판응집률(血少板凝集率)을 증가(增加)시켰다. 그중 <TEX>$K^+-free$</TEX>에 처치(處置)한 것이 가장 강(强)하게 작용(作用)하였다. 5) Sodium nitropusside로 전처치(前處置)하였을 때 <TEX>$K^+-free\;PSS$</TEX>에 처치(處置)된 혈관(血管)에 의한 항응집반응(向凝集反應)은 용량(用量)에 의존(依存)하여 현저(顯著)히 억제(抑制)되었으며 hydralazine이나 diazoxide에 의하여는 아무런 작용(作用)을 받지 아니하였다. 6) Dibutyryl cyclic AMP 및 8-bromo cyclic GMP 모두 혈소판응집률(血小板凝集率)을 감소시켰고, 후자(後者)는 전자(前者)에 비(比)하여 월등(越等)히 현저(顯著)하였다. sodium nitroprusside에 의한 항응집률(抗凝集率)은 methylene blue 전처치(前處置)에 의하여 길항(拮抗)되었으나, bovine hemoglobin전처치에 의하여는 영향(影響)을 받지 아니하였다. 이상(以上)의 성적(成績)을 종합(綜合)하면, 뇌졸중증(腦卒中症)때, 특히 뇌혈전증(腦血栓症)의 응급치료시(應急治療時) sodium nitroprusside의 응용(應用)이 가능(可能)하다고 사료(思料)되며, 이에 대(對)하여 임상적(臨床的) 치료(治療)가 기대되는 바이다. It was undertake to investigate the factors involved in the micro thrombus formation in the plasma from the patients with cerebrovascular disease(CVD) and the in vitro actions of sodium nitroprusside on the platelet aggregate formation. 1) The microthrombus formation in the plasma from CVD was significantly enhanced, in comparison with that from the healthy volunteers. 2) Both lipid peroxide and cathepsin D in the plasma from CVD were higher than those levels from the healthy volunteers. 3) Whereas the platelets from healthy individuals showed less aggregation activity in response to ADP in the second phase those from CVD revealed the enhanced aggregating response to ADP. 4) When the bovine basilar artery, rabbit aorta and human umbilical artery were pretreated with <TEX>$K^+-free$</TEX> PSS, ouabain, 13-hydroperoxylinoleic acid(13-HPLA) and cadmium they markedly enhanced the platelet aggregability respectively. 5) Platelet aggregation induced by <TEX>$K^+-free$</TEX> PSS-treated bovine basilar artery was decreased by sodium nitroprusside in a dose-dependent manner, but not by either hydralazine. 6) Both dibutyryl cyclic AMP and 8-bromo cyclic GMP had the inhibitory action on the platelet aggregation. However, the latter had more prominent action than former. The antiaggregating effect by sodium nitroprusside was antagonized by pretreatment with methylene blue, but not by hemoglobin. These results provide the evidences for the therapeutic use of sodium nitroprusside in the emergency of cerebrovascular disease and in remains the further study of the clinical therapy with it.

카드뮴중독(中毒) 가토(家兎)의 동정맥(動靜脈)에 관하여

홍기환 大韓藥理學會 1979 대한약리학잡지 Vol.15 No.1

Cd 중독(中毒)으로 가토(家兎)에 고혈압(高血壓)을 일으키고 이를 중독(中毒) 가토(家兎)와 대조(對照) 비중독(非中毒) 가토(家兎)의 간문맥(肝門脈), 흉부(胸部) 대정맥(大動脈) 및 폐정맥(肺動脈)을 적출하여 in vitro에서 장력(張力)-길이의 관계를 관찰하고 NE, <TEX>$K^+$</TEX>-depolariration 및 angiotensin의 수축(收縮) 효과(效果)를 비교(比較)하였다. 1. 비중독(非中毒) 대조군의 평균 동맥압은 <TEX>$87.0{\pm}4.7mmHg$</TEX>이었고 Cd 중독군(中毒群)에서는 <TEX>$109.0{\pm}2.8mmHg$</TEX>로 Cd 중독(中毒)에 의하여 의의(意義)있는 증가(增加)를 나타내었다. 2. 동(動) 정맥(靜脈)의 장력(張力)-길이 곡선(曲線)에서 Cd 중독(中毒) 가토의 간문맥(肝門脈)은 passive tension의 증가에 따라 distensibility가 현저히 증가되나 대동맥(大動脈)에 있어서는 오히려 대조군에 비(比)하여 유의(有意)한 감소(減少)를 나타내었고 폐동맥(肺動脈)은 아무런 차이(差異)를 나타내지 아니하였다. 3. Passive tension의 증가에 따른 active tension의 변화(變化)는 대동맥(大動脈)에 있어서는 Cd중독으로 현저히 감약되었으나 간문맥(肝門脈)이나 폐동맥(肺動脈)은 대조군과 별다른 차이(差異)가 없었다. 4. Cd 중독군(中毒群)이나 비중독(非中毒) 대조군에서 passive tension의 변동에 따른 수축결과(收縮效果)는 세 혈관에서 모두 유의(有意)한 차이를 나타내지 아니하였다. 5. 대동맥(大動脈) 절편에 대한 angiotensin의 수축(收縮) 효과(效果)는 Cd 중독군(中毒群)에서 유의하게 저하되었다. The tension-length relationships and reactivity of vascular smooth muscle in longitudinal strips from portal vein ana in helical strips from thoracic aorta and pulmonary artery of normotensive control and cadmium-hypertensive rabbits were studied in vitro. 1. The mean arterial pressures of non-poisoned control rabbits was <TEX>$87.0{\pm}4.7 mmHg$</TEX>. However, Cd-poisoned group revealed the significant increase in pressure by <TEX>$109.04{\pm}2.8 mmHg$</TEX> (p<0.005). 2. By tension-length studies, strips from portal vein of Cd-poisoned group stretched a greater percent increase in length in response to an applied resting force from 0.25 to 5 g than did those from non-poisoned group. On the contrary, strips from thoracic aorta of Cd-poisoned group showed less compliant than those from control, i.e. the former underwent a less percent increase in length than the latter. Passive tension-length relations of pulmonary artery was unaffected by Cd-hypertension. 3. The force of contraction(active tension) was significantly lowered in strips from aorta of Cd-hypertensive group throughout the range of <TEX>$0.5{\sim}2g$</TEX> passive tension. However, there was no significant difference in the development of active tension of portal vein or pulmonary artery of both groups. 4. The <TEX>$K^+$</TEX>-contraction in the portal vein, aorta and pulmonary artery of Cd-poisoned group made no difference in the active force from those of control group. 5. The force of contraction in the strips from aorta of Cd-poisoned group was significanty decreased compared to that of control. The results suggested that the alterations in vascular reactivity to contracting substances and in distensibility to passive tension were induced in the Cd-hypertensive rabbits.