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제1위 섬모충(rumen ciliates)을 이용한 동물성 단백질(치어용 사료) 개발
지차호,현공율,Jee, Cha-ho,Hyun, Gong-yool 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.2
This study was carried out to develop the animal protein(feed for fry) that was isolated, purified and lyophilized the rumen ciliates from the healthy rumen contents which have $10^5-10^6/g$ ciliates and were discarded in abattoirs. The rumen ciliates are non-pathogenic, anaerobic and the weight of this protozoa is 2% of rumen content. The rumen protozoan and bacterial proteins both have a biological value for rats of 80-81, which is higher than the 72 of brewer's yeasts. Furthermore, the true digestibility and net protein utility of the protozoan protein are 91 and 73, much higher than those of bacterial(74 and 60) or yeast(84 and 60) proteins. The amino acids of rumen protozoa is nutritionally superior than the others. The size of rumen ciliates is $30-200{\times}20-110{\mu}m$ and so we had isolated and purified the rumen ciliates from the rumen contents by the physical methods. The purified rumen protozoa was lyophilized with freezing dryer. The results of this experiment were as follows : 1. Population dynamics of protozoan ciliates in slaughtered rumens; % of samples which small ciliates were predominated was 82.5%(52/63) and that of large ciliates was 17.5%(11/63). 1) predominant species of small ciliates were Entodinium ovinum and E nanellum. 2) predominant species of large ciliates were Epidinium ecaudatum and Diploplastron affine. 2. The lyophilized rumen ciliates which were isolated and purified from 1 kg of rumen content at the pH 6.2-6.8 was about 7.0 gram. 3. The nutrient analysis of lyophilized rqmen ciliates(LRC) was as follows: 1) Proximate analysis of the LRC and the composition of fry feed; moisture 8.05%(below 10.0), protein 35.37%(45), fat 5.39%(4.5), fiber 1.23%(below 2.5), ash 2.25%(below 15.0), Ca 0.26%(below 2.0), P 0.14%(below 1.1), energy 4,608.11(fish meal 5000 cal/g) 2) Amino acids (% in crude protein) of the LRC and the rotifer(Brachionus plicatilis); Arg 5.19%(4.50), His 2.50%(1.55), Ile 5.29%(3.45), Leu 8.11%(5.85), Lys 10.34%(6.15), Met 2.25% (0.85), Phe 5.66%(3.80), Thr 5.14% (3.45), Val 4.18%(3.90), Ala 4.13%(3.35), Asp 13.26%(8.25), Glu 16.62%(9.20), Gly 4.23%(3.10), Pro 3.25%(5.05), Ser 4.85%(3.85), Tyr 5.04%(3.05) 3) Fatty acids(% in fat) of the LRC and the rotifer(biological feed ; Brachionus plicatilis); myristic acid(C14:0) 3.27%(0.3), myristoleic acid(C14:1) 0.83%(-), palmitic acid(C16:0) 39.11% (23.5), palmitoleic acid(C16:1) 2.81%(2.0), stearic acid(C18:0) 9.36%(5.6), oleic acid(C18:1) 25.54%(3.5), linoleic acid(C18:2) 15.05%(32.9), linolenic acid(C18:3) 1.74%(9.8). Judging from the above investigated results, the analytical data of proximate analysis, amino acids, fatty acids of the purified and lyophilized rumen protozoa are reasonable for the feed of freshwater fishes(fry and fingerling). But it was disappointed of our expectation that the crude protein of lyophilized rumen ciliates contains low percentage, it was thought that because of the small ciliates(starch digester) in beef cattle rumens which were administered the concentrated feed, is much difficult to isolate and purify than the large ciliates(fiber digester).
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녹차부산물을 이용한 기능성 축산물 증명에 대한 분자생물학적 접근
강신석 ( Shin Seok Kang ),현공율 ( Gong Yul Hyun ),최해연 ( Hae Yeun Choi ),조우영 ( Woo Young Cho ),김태융 ( Tae Yung Kim ),강신권 ( Shin Kwon Kang ),강정부 ( Chung Boo Kang ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.2
Green tea was known which regulated adipocyte differentiation metabolism. The mecha nism on the lipid decreased contents of TAG in the plasma. In addition, green tea increased the expression leptin mRNA, PPAR δ mRNA and TGF β. The tea tested was korean powdered green tea. In this experiment, Sprague-Dawley(SD) rats were fed 3 % green tea(powdered) for 3 weeks on the basal diet and obese diet and green tea grouts-fed pork meats, duck meats. The expression of leptin mRNA and PPAR δ mRNA were up-regulated in the green tea-fed groups compared with those of the not green tea-fed groups. There were no significantly difference on the expression of leptin mRNA and PPAR δ mRNA in green tea grouts-fed pork meats, duck meats as compared with the not fed green tea grouts meats. TGF β mRNA, TNF α mRNA and adipsin mRNA were not expressed in the pork meats, duck meats. The expression of TGF β mRNA, TNF α mRNA and adipsin mRNA were observed in the experimental rats but no significantly difference on the contents. Physiologic regulated genes were not expressed in the green tea grout-fed pork meats and duck meats.
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HPLC를 이용한 carbamate계 농약의 다성분 동시분석
송영각 ( Young Gak Song ),서영철 ( Young Chul Seo ),현공율 ( Gong Yul Hyun ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ) 한국가축위생학회 2003 韓國家畜衛生學會誌 Vol.26 No.2
To explore the most sensitive and useful method for simultaneous determination of 8 carbamate pesticides in livestock productions, the mixture of 8 carbonates was determined by HPLC with scanning fluorescence detector. For mobile phase water, acetonitrile and methanol program gradient showed more applicable sensitivity than water and acetonitrile program gradient used ExW 339nm and EmW 445nm for fluorescence detector. On using carbamate columns, the retention time was within 4 to 20 minutes. And this made it possible to separate and detect simultaneously. Therefore we could analyze it exactly and efficiently and reduce time. The preparation process of MSPD method showed that the high recovery rate was more than 88.5% in most of the carbamate pesticides. Therefore we could say that it was an efficient and fast method analyzing out of lots of samples.
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꽃사슴의 Clostridium perfringens A형에 의한 장독혈증 발생보고
이청산 ( Cheong San Lee ),한성태 ( Sung Tae Han ),곽학구 ( Hak Koo Kwak ),박경재 ( Kyung Jae Park ),현공율 ( Gong Yul Hyun ),조우영 ( Woo Young Cho ),이종인 ( Jong In Lee ),배유찬 ( You Chan Bae ),진영화 ( Yong Hwa Jean ) 한국가축위생학회 2002 韓國家畜衛生學會誌 Vol.25 No.2
The case reports for clostridium perfringens type A enterotoxemia in Formosan deer have rarely been reported. This paper describes a natural case of type A enterotoxemia in farmed Formosan deer in Cheongwon-gun. A dead, male 10-month-old Formosan deer was submitted to Chungbuk Livestock and Veterinary Research Institute, March 24, 2001 and examined. That deer was fed with assorted grain feed, oak leaves, acorn and bean curd. Grossly there was no visible external change. Despite of the carcass being examined within 12 hours of death, there was a quite degree of postmortem decomposition. There was severe hemorrhage in the serosa of abomasum and small intestine. Much blood tinged and watery contents were contained in those organs. Also there were severe swelling of spleen, some red foci in hepatic parenchyma. Microscopically there were severe congestion and hemorrhage in mucosa, submucosa, muscular layer, and serosa of abomasum and small intestine. Also spleen and pancreas showed severe congestion and hemorrhage. There were multifocal hemorrhage with hepatic necrosis in periportal area and focal mononuclear cell deposition in sinusoid. In bacterial culture for small intestine, Cl peifringens was isolated. By toxin typing for the strain, that had α-toxin belonged to type A. In electronmicroscopy for feces, no virus particle was detected. Considering clinical signs, gross lesions, microscopic lesions, bacterial culture, and toxin typing of the isolate, this case was diagnosed as enterotoxemia by Cl perfringens type A.
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