성분리 키트가 처리된 소정자를 이용한 체외수정란의 배양과 성분리 효율

허영태,김동구,엄상준,Heo, Young-Tae,Kim, Dong-Gu,Uhm, Sang-jun 한국수정란이식학회 2018 한국동물생명공학회지 Vol.33 No.3

It has been claimed that artificial insemination (AI) of cows with frozen-thawed semen treated with commercially produced kits, Wholemom (in favour of female gender) increases the birth chance of calves with desired sex ratio by approximately 85% without decrease of pregnancy rates. Hence, this study was conducted to investigate the efficacy of wholemom kits as combined with frozen-thawed bovine semen during in vitro fertilization on the in vitro fertilization and developmental efficiency and sex ratios such as some reproductive parameters in bovine. For this, 1,737 oocytes were in vitro fertilized and developed. Agglutination effects on bovine after treatment of Wholemom kit were observed by time passage and dose respectively. To determine sex of embryos, Bovine embryo Y-specific gene primers(ConEY) and Bovine specific universal primer(ConBV) were used as multiple PCR method. Fertilization rate of wholemom-treated group was significantly lower than its of control group[66.9% (1,156/1,737) in Wholemom-treated group; 75.0% (610/813) in control group]. However, developmental rate after fertilization of both wholemom-treated and control groups were not significantly different [26.1% (404/1,156) in Wholemom-treated group; 27.4% (224/610) in control group]. Sex ratio of in vitro fertilized embryo with frozen-thawed semen treated with wholemom kit was determined by multi PCR. Female ratio in wholemom-treated group [85.4% (173/201)] was significantly higher than its of control group [47.2% (66/141)]. In conclusion, wholemom treatments of semen used in the in vitro fertilization and development of bovine oocytes provided increase in female ratio with decrease of fertilization rate.

소 초유의 유효성분과 산업적 활용

서상아,서혜령,허영태,Seo, Sang-Ah,Seo, Hye-Ryoung,Heo, Young-Tae 한국수정란이식학회 2018 한국동물생명공학회지 Vol.33 No.3

Bovine colostrum is necessary for newborn calves to survive, grow and receive immunity from their mother. Cows in Korea produce about 35kg of colostrum, 4Kg of which is fed to the calf, and the rest is discarded. The bovine colostrum causes the harmful side effects to human, such as allergies and digestive problems; so, it is prohibited by law to consume colostrum itself as a food. However, many scientific research data have suggested that components in the colostrum can improve human health and has the ability to help treat diseases. In line with the trend of food and pharmacy industries using natural product materials, which attract positive attention, recently, some ingredients in colostrum have been used in the production of food supplements, and it has been used in its raw form in some cosmetics. This review introduces the active ingredients and physiologically active substances contained in bovine colostrum, summarizes the efficacy of physiological enhancement of the colostrum, which has been proven by scientific methods to date, and also suggests the possibility of industrial applications of colostrum as an animal-derived natural material.

도시형 자기부상열차 분기기 구간의 제3궤조 전차선 시스템 개발

민병찬(Byong-Chan Min),허영태(Young-Tae Heo),홍두영(Du-Young Hong),이원주(Won-Joo Lee),조수연(Su-Yeon Jo),정남철(Nam-Cheol Jeong) 한국철도학회 2011 한국철도학회 학술발표대회논문집 Vol.2011 No.10

The third-rail system is an important device supplying power directly to the Maglev train through physical contact with the collecting shoe. It is directly related to safety and reliability for the running of Maglev. However most the third-rail system used in Korea depend on foreign product or technologies Korea Urban Maglev in the development of appropriate power feeding is urgent. In particular the turnout section is the weakness point in the system because bending force by turnout section movement and fatigue caused by repetitive motion as well as the expansion by temperature the forces by Maglev collecting shoe is added th the third-rail. Therefore this paper proposes the third-rail system appropriate for Korean Urban Maglev of turnout section. To verify the structural stability of POSCO ICT third-rail system the finite element analysis and physical testing was performed. The third-rail is fixed on each side of the turn-out section steel structure by epoxy insulation supporter and the integral behaviors are occurred. Therefore the maximum horizontal displacements of each support are investigated and then it is applied to finite element model of the third-rail to investigate the moments and stress. Also the bending test about one million times and Expansion Joint for the third-rail was performed. The third-rail system safety and reliability was identified by test line on Korea Institute of Machinery & Materials in Deajeon for under the actual usage environment such as the Maglev and turn-out operation.

유아교육에서 창의성 발달은 어떻게 적용될 수 있는가?

박춘성 ( Park Choon-sung ),허영태 ( Heo Young-tae ) 한국실천유아교육학회 2019 실천유아교육 Vol.24 No.1

본 연구는 창의성 슬럼프와 발달초기의 유아 창의성 연구에 대한 제언을 목적으로 연구를 진행하였다. 창의성 슬럼프는 창의성의 개념이 정립되면서 나타난 개념이며 주요한 내용은 초등학교 4학년 시기에 창의성이 급격히 감소한다는 것이다. 이후의 후속 연구를 통하여 다양한 논쟁이 있었는데, 슬럼프가 나타난다는 견해, 슬럼프가 나타나지 않는다는 견해, 슬럼프라기보다는 다른 양상이라는 견해 등 다양한 논의가 진행되었다. 지금까지의 창의성 슬럼프의 연구를 종합적으로 검토한 연구들이 최근에 등장하였는데, 창의성 슬럼프 연구결과들이 각기 다른 이유를 창의성 측정도구, 연구방법론의 문제, 국가 간 창의성 발달 차이 등에서 찾을 수 있다. 유아교육도 창의성 연구를 위해서는 지적된 문제점을 해결하여야 하는데, 창의성 측정도구, 횡단적 연구의 한계 극복, 인과관계 설명으로 요약하면서 연구를 마쳤다. The purpose of this study is to propose the creativity slump and early childhood creativity research. Creativity slump is a concept that has emerged as the concept of creativity has been established. The main content is that creativity declines dramatically in the fourth grade of elementary school. There were various controversies in subsequent researches, such as the opinion that a slump appears, the opinion that a slump does not appear, and the opinion that it is a different aspect than a slump. Research on creativity slump has been introduced recently. The results of creativity slump research are different from those of creativity measurement tool, research methodology, and difference of development of creativity between countries. In the early childhood education, it is necessary to solve the problems pointed out for the creativity research. The research is completed by summarizing the creativity measurement tools, the limit of the cross-sectional study, and the explanation of the causal relationship.

영재들의 정서행동문제와 반려동물 효과에 대한 가능성 탐색

박춘성(Park, Choon-Sung),허영태(Heo, Young-Tae) 한국영재교육학회 2020 영재와 영재교육 Vol.19 No.2

본 연구는 영재학생의 정서행동문제에 반려동물을 활용한 치료 가능성을 탐색하기 위한 방안을 살펴보는데 목적이 있다. 아직 동물을 매개로한 치료는 생소한 분야이기 때문에 가능성에 대한 접근을 하였다. 이를 위해 영재와 일반학생의 정서행동장애의 특성을 밝히고 반려동물이 정서행동장애에 미치는 영향력의 선행 연구를 살펴보았다. 반려동물이 정서행동치료에 긍정적인 영향을 주는 것을 확인하였지만, 국내의 반려동물 매개치료에 대한 연구는 매우 미진함을 밝히면서 5개 분야의 반려동물 매개 치료의 영역을 밝혔다. 연구영역은 반려동물의 양육 여부에 따른 학습자 특성 및 정서문제의 관련성에 대한 분석, 종단적인 효과분석, 발달별 권장 반려동물, 반려동물의 긍정적, 부정적 효과, 반려동물 활용 프로그램의 개발을 제안하였다. This study was conducted to explore the emotional behavioral problems of gifted students through their companion animals. While identifying the characteristics of emotional behavioral disabilities between gifted and general students, we investigated the precedent study of the influence of companion animals on emotional behavioral disabilities while examining the differences between emotional and disability between gifted and general students. Although it was confirmed that the companion animal has a positive effect on the emotional behavior therapy, studies on companion animal-mediated treatment in Korea revealed a very low level and revealed the areas of companion animal-mediated treatment in five fields. The research area suggested the analysis of the relationship between learner characteristics and emotional problems depending on whether or not a pet is reared, longitudinal analysis, and the development of a recommended companion animal for each development, a companion animal s positive and negative effects, and a companion animal utilization program.

동결-융해된 인간 배반포기 배 유래의 배아 간(幹) 세포 배양

김은영,남화경,이금실,박세영,박은미,윤지연,허영태,조현정,박세필,정길생,임진호,Kim, Eun-Young,Nam, Hwa-Kyung,Lee, Keum-Sil,Park, Sae-Young,Park, Eun-Mi,Yoon, Ji-Yeon,Heo, Young-Tae,Cho, Hyun-Jung,Park, Se-Pill,Chung, Kil-Saeng,Lim, Jin 대한생식의학회 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.1

Objective: This study was to establish the human embryonic stem (ES) cells derived from frozen-thawed blastocyst stage embryo that were destined to be discarded after five years in routine human IVF-ET program. Methods: Frozen-thawed and survived human blastocysts were treated by immunosurgery, and recovered ICM cells were cultured onto STO feeder cell layer and ICM colony was subcultured by mechanical dissociation into clumps. To identify ES cell, alkaline phosphatase staining and expression of Oct4 in replated ICM colonies were examined. Also, to examine the possibility of ES cell differentiation, retinoic acid (RA), basic fibroblast growth factor (b-FGF), nerve growth factor (NGF) were added in culture medium. In addition, to classify the specific cell type, differentiated cells were stained by indirect immunocytochemistry. Results: One ICM colony recovered from frozen-thawed six blastocysts was subcultured, continuously replated during 40 passage culture duration without differentiation. Subcultured colonies were strong positively stained by alkaline phophatase. When the expression of Oct4 in cultured ES colony was examined, Oct4b type is more clearly indicated than Oct4a one although there was not detected in embryoid body or differentiated cells. In differentiated cardiomyocytes from ES colony, cells were beaten regularly (60 times/min). In differentiated neural cells from ES colony, neurofilament (NF) 200 kDa protein, microtubule associated protein (MAP) 2 and ${\beta}$-tubulin of specific marker in neurons, glial fibrillary acidic protein (GFAP) of specific marker in astrocytes and galactocelebrocide (GalC) of specific marker in oligodendrocytes were confirmed by indirect immunocytochemistry. Also, muscle cells were detected by indirect immunocytochemistry. In addition, ES colonies can be successfully cryopreserved. Conclusion: This study suggested that establishment of human ES cells can be successfully derived from frozen-thawed blastocysts that were destined to be discarded, and obtained specific cell types (cardiomyocytes, neurons and muscle cells) through the in vitro differentiation procedures of ES cells.

Optimization of In Vitro Culture System of Mouse Preantral Follicles

박은미,김은영,남화경,이금실,박세영,윤지연,허영태,조현정,박세필,임진호,Park, Eun-Mi,Kim, Eun-Young,Nam, Hwa-Kyung,Lee, Keum-Sil,Park, Sae-Young,Yoon, Ji-Yeon,Heo, Young-Tae,Cho, Hyun-Jung,Park, Se-Pill,Loo, Jin-Ho The Korean Society for Reproductive Medicine 2001 Clinical and Experimental Reproductive Medicine Vol.28 No.2

연구목적: 본 연구는 생쥐 preantral follicles의 체외 배양 조건을 확립하고 이를 기초로 높은 체외 발달률 그리고 산자 생산률을 얻고자 하였다. 연구재료 및 방법 : Preantral follicles의 oocyte-granulosa cell complexes (OGCs)는 생후 12일된 FI ($C57BL{\times}CBA$)으로부터 난소를 적출하여 효소를 이용한 방법을 통해 획득하였다. 회수된 complexes는 10일 또는 12일 동안 체외 성장을 위해 Transwell-COL membrane insert로 옮겨졌고 5% FBS, 100 mIU/ml FSH, 100 mIU/ml hMC가 첨가된 ${\alpha}MEM$에서 배양되었다. 체외 성숙을 위해 1.5 IU/ml hCG가 첨가된 ${\alpha}MEM$에서 18 hrs 배양을 실시하였다. 그 후 M16에서 수정능력이 획득된 정자와 수정을 하여 4 hrs, 7 hts, 9 hrs 후에 10% FBS가 첨가된 modified M16 배양액에서 4일간 배양하거나 또는 bovine cumulus cell과 co-culture를 실시하였다. 그리고 형태적으로 정상적인 22개의 상실배와 포배를 2마리의 위임신 대리모 (ICR)의 자궁에 이식하여 산자 생산을 유도하였다. 결과: 1) OGCs 크기가 mouse preantral follicles의 핵 및 세포질 성숙에 미치는 영향을 조사하였을 때 $120{\sim}150{\mu}m$의 preantral follicles (MII: 33.0%, 난할률: 36.7%, 상실배 이상; 20.9%)은 핵 및 세포질 성숙에 있어서 $70{\sim}110{\mu}m$ (MII: 12.2%, 난할률: 10.2%, 상실배 이상: 4.8%)보다 더 높았다(p<0.001). 2)체외 성장기간의 연장이 mouse preantral follicles의 핵 및 세포질 성숙에 미치는 영향을 조사하였을 때 10일 (난할률: 38.2%)은 12일 (난할률: 20.0%)보다 난할률에서만 더 높았다 (p<0.01). 3) 체외 수정 시간의 연장이 mouse preantral follicle의 세포질 성숙에 미치는 영향을 조사하였을 때 9 hrs (난할룰 31.5%, 상실배 이상: 14.3%)은 4 hrs (난할률: 17.5%, 상실배 이상: 4.8%), 7 hrs (난할률: 20.4%, 상실배 이상: 6.1%) 보다 세포질성숙에 있어서 유의하게 높은 발달률을 나타냈다 (p<0.01). 4) 공배양이 mouse preantral follicle의 세포질성숙에 미치는 영향을 조사하였을 때 공배양 (상실배 이상: 17.4%)을 실시했을 때와 M16 (상실배 이상17.4%)에서 배양되었을 때는 차이가 없었다. 5)preantral follicle의 크기 ($120{\sim}150{\mu}m$), 체외 성장기간 (10일), 체외 수정 기간 (9시간), 배양 환경 (단지 medium만 존재)의 적절한 결과들을 종합하여 수행하였을 때 MII 성숙률, 난할률, 상실배 이상의 발달률은 30.2%, 39.3%, 22.5%이었고 총 22개의 상실배 및 포배를 2마리의 대리모에 이식했을 때 1마리가 임신했고 1마리의 산자를 생산했다. 결론: 따라서, 본 실험은 preantral follicle을 이용한 체외 배양 시스템이 생쥐 oocyte를 공급하는 또 다른 방법으로 효과적으로 이용될 수 있다는 것을 시사한다.

도시형 자기부상열차 제3궤조 전차선로의 안전성 및 신뢰성 확보를 위한 인터페이스 정립 (The Organization of Interface for safety and reliability of Urban Maglev Third rail System)

민병찬(Byong-Chan Min),조상훈(Sang-Hoon Cho),허영태(Young-Tae Heo),홍두영(Du-Young Hong),김창환(Chang-Hwan Kim),정남철(Nam-Cheol Jeong) 한국철도학회 2011 한국철도학회 학술발표대회논문집 Vol.2011 No.5

도시형 자기부상열차는 전자기력으로 레일에서 낮은 높이로 부상하여 운행되는 열차이다. 자기부상열차는 궤도와의 직접적인 접촉 없이 움직이기 때문에 소음이 적고 기계적 마찰에 의한 진동이 거의 발생하지 않는다. 또한 급곡선 주행이 가능하고 경사도가 높은 언덕지역도 쉽게 주행 할 수 있기 때문에 도심에서의 새로운 교통수단으로 주목받고 있다. 따라서 도시형 자기부상열차의 성공적인 실용화를 위하여 제3궤조 전차선로 설비와 인터페이스를 통하여 안전성과 신뢰성 확보는 필수 고려 사항중 하나이다. 특히 파워레일, 신축확장장치, 절연 구분장치, 지지금구와 같은 제3궤조 전차선로 설비는 내 부식성, 시공의 편의성 및 유지보수성을 고려하여 설치되어야 한다. 본 논문에서는 자기부상열차 시스템 구축을 위한 제3궤조 설비의 구성요소에 대한 인터페이스 항목을 분석하고 전차선로의 안전성을 높일 수 있는 시스템 엔지니어링 인터페이스 체계를 제시하고자 한다. 또한 제안하는 자기부상용 제3궤조 전차선로 시스템은 한국기계연구원내 시험을 통하여 시스템의 안전성을 증명하였으며, 시스템 구성품의 수명분석을 통해 제3궤조 전차선로 운영의 신뢰성을 향상시키고자 한다. The Maglev train is operated by levitating from a power of a large number of magnets and moving without direct contact to railway track so that reduces noise and vibration due to mechanical friction. Also, the Maglev passes sharp curves and steep hill without any difficulties. The Maglev has a potential to be an alternative transport system urban areas. For successful commercializing of Maglev, the organization of interface for safety and reliability of third rail system are one of the key considerations. Especially, the components of the third rail system, such as power rail, expansion joints, FRP section insulator, and supporter with epoxy insulator, should be durable, convenient for construction, and easy to maintenance. This paper analyzes the characteristics of the third rail system components and proposes organization of interface for system engineering. The operating tests of KIMM for the proposed third rail system verify the safety. Also, this paper analyzes the life cycle of the system components to improve the system reliability and evaluation.

성격분류에 기반한 영재들의 반려견 선택을 위한 일연구

유경원(Kyoung-won You),황보은(Bo-Eun Hwang),엄상준(Sang-Jun Uhm),박춘성(Choon-Sung Park),허영태(Young-Tae Heo) 한국영재교육학회 2021 영재와 영재교육 Vol.20 No.2

이 연구에서는 영재들의 심리적인 문제점을 해소할 수 있는 방안의 하나로 반려견을 활용하여 인간의 성격과 반려견종을 매칭 할 수 있는 방법을 고안하고자 하였다. NEO 성격검사의 5가지 요소들을 반려견 양육과 특성에 맞추어 적용하였으며, 각각의 요소별로 반려견의 품종을 분류하여 반려견 양육 희망자의 성격에 부합하는 반려견품종 매칭 가이드라인을 작성하였다. 반려견 양육 희망자의 성격과 부합되는 반려견 품종의 매칭을 위해 인간의 성격 요인을 분석하는 기존의 방법들을 검토하였으며, 반려견 양육요소에 적용이 가장 적절한 5요인(신경증, 외향성, 개방성, 친화성, 성실성) 성격검사를 반려견 양육에 부합되도록 적용하였다. (사)한국애견협회의 등록 견종 상위 50위를 선별하여 각 견종의 고유 성격과 양육 특징을 인간의 5가지 성격 요소별로 분류하였다. 이러한 연구는 선행되어지지 않은 최초의 시도이기에 신뢰도의 확보를 위해서 지속적인 보완작업과 수렴타당도, 판별타당도 등의 타당화 작업이 수행될 필요가 있으며, 본 연구에서 개발된 결과의 타당성 검증을 위한 후속 연구를 수행하여, 신뢰성과 편리성이 있는 인간 성격에 부합하는 반려견 선택 도구를 제시하고자 한다. In this study, five each elements of the NEO personality test were applied according to the companion dog rearing and characteristics, and guideline to choice breed of companion dog was presented based on the personality of the dog rearing applicant. The literature review presented the issue of abandonment and disruption of companion animal, and investigated policies and systems for suppression of abandonment and disruption rates of companion animals in abroad. Methods of analyzing human personality were reviewed and five factors (Neuroticism, Extraversion, Openness to Experience, Agreeableness, Conscientiousness) were applied for matching prospective adopter and specific dog breed which following their characters. The top 50 registered dogs of the Korea Pet Association were selected and the unique personality and parenting characteristics of each dog were classified based on five characteristic elements of humans. Since these studies are the first attempt not to be preceded, continuous complementary and convergent feasibility and validation work need to be carried out to ensure reliability, and follow-up studies to validate reliable dog breed secection system to character of prospective adoptors.