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16S-3S rRNA Intergenic Spacer Region을 이용한 Vibrio fluvialis의 검출
강현실 ( Kang Hyeon Sil ),허문수 ( Heo Mun Su ),이제희 ( Lee Je Hui ) 한국환경생물학회 2003 환경생물 : 환경생물학회지 Vol.21 No.1
We have examined the 16S rRNA intergenic spacer regions(ISR) of Vibrio Fluvialis. ISRs were PCR amplified, cloned into a plasmid vector and then sequenced. As results of ISR nucleotide sequence analysis, total of 6 clones were isolated depending on the size. The clones were different in both the number and the composition of the tRNA genes, and were designated ISR-A, ISR-E, ISR-E1, ISR-IA, ISR-EKV, ISR-EKAV. ISR-A contains tRNA^(Ala) ; ISR-IA, tRNA^(Ile)-tRNA^(Ala) ; ISR-EKV, tRNA^(Glu)-tRNA^(Lys)-tRNA^(Val) ; ISE-EKAV, tRNA^(Glu)-tRNA^(Lys)-tRNA^(Ala)-tRNA^(Val) ; ISR-E and E1, tRNA^(Glu) clusters. ISR-EKV was shown to be a minor type out of the six ISR tyoes and showed a very limited homology between ISR-EKV from V. Fluvialis and ISRs from other Vibrio species. Therefore ISR-EKV sequence was used to design species-specific primers to detect V. fluvialis from other Vibrio species by PCR reaction . The specificity of the primers was examined using genomic DNA of other Vibrios as Templates for PCR reaction. The result showed that PCR can be a useful method to detect V. fluvialis among Vibrio species in a single PCR reaction.
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단보 : 제주도 양식넙치, Paralichthys olivaceus의 스쿠티카충병 발생 동향
진창남 ( Jin Chang Nam ),이창훈 ( Lee Chang Hun ),오상필 ( O Sang Pil ),정용욱 ( Jeong Yong Ug ),송춘복 ( Song Chun Bog ),이제희 ( Lee Je Hui ),허문수 ( Heo Mun Su ) 한국어병학회 2003 한국어병학회지 Vol.16 No.2
The research was carried out to know the occurrence trend of scuticoeiliatosis and mortality of fingerlings in flounder. The diseased fish were collected from on-land rearing farms and hatcheries of flounder in Jeju Island from January l99l to December 20
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