Effects of Diallyl Disulfide on the Hepatic Glutathione Peroxidase Activity in Rat

허근,이상일,박종민,Huh, Keun,Lee, Sang-Il,Park, Jong-Min The Korean Society of Pharmacology 1986 대한약리학잡지 Vol.22 No.2

Glutathione peroxidase might play an important role in the protection of cellular structures against oxidative challange by hydrogen peroxide and several organic hydroperoxides. It is widely accepted that allicin is biological active component of garlic, and allicin is easily degraded to diallyl disulfide and other components. This study was attempted to elucidate the effect of diallyl disulfide on some biological activities. It was observed that the activity of serum transaminase and glutathione level in liver were not changed by the treatment of diallyl disulfide. The liver cytosolic glutathione peroxidase activity was significantly enhanced. Whereas, mitochondrial enzyme activity was slightly increased. In the presence of diallyl disulfide in vitro, $V_{max}$ value of glutathione peroxidase for hydrogen peroxide was increased. On the other hand, Km value was not changed. 마늘성분중의 하나인 diallyl disulfide가 간 glutathione peroxidase 활성에 어떠한 영향을 주는가를 관찰하여 다음과 같은 성적을 얻었다. 여러 농도의 diallyl disulfide를 흰쥐에 투여 하였을때 혈청 transaminase의 활성은 변동이 없었으며 또한 간 glutathione의 함량도 변화되지 않았다. 그러나 간 가용성분획의 glutathion peroxidase 활성은 현저히 증가되었다. 시험관 내에서 간 가용성분획의 glutathione peroxidase의 활성은 $15{\mu}g/m{\ell}$ 이상의 diallyl disulfide를 첨가 하였을때 현저히 증가되었으며, 동력학적인 측면에서 관찰하였을때 diallyl disulfide$(20{\mu}g/m{\ell})$에 의해 Km치는 별 변화가 없었으나 Vmax치는 현저히 증가되었다. 이상의 실험결과에서 마늘 성분 중에 glutathione peroxidase 활성을 증가시키는 성분은 allicin의 분해산물인 diallyl disulfide에 기인된 것으로 사료되며 또한 이 성분은 효소의 기질결합 부위가 아닌 다른 부위에서 효소의 활성을 조절해 줄 것으로 생각되어진다.

미역의 자궁수축에 관한 연구

허근,송재웅,최종원,Huh, Keun,Song, Jae-Woong,Choi, Jong-Won 한국생약학회 1992 생약학회지 Vol.23 No.3

Undaria pinnatifida (UP) has long been ingested by woman who was delivered of a child. The present study was investigated to find out the contractive compound from UP and the contractive mechanism. The active extract, MeOH extract was partitioned with $CHCl_3$ and water. Whereas potassium iodide and alginic acid do not affected the uterus contraction, $CHCl_3$ extract significantly increased uterus contraction in vitro and in situ studies. The contraction by $CHCl_3$ extract was not blocked by atropine, prazosin and propranolol. Blood estrogen level was not changed by $CHCl_3$ extract.

Effect of Sinigrin on the Hepatic Xanthine Oxidase and Free Radical Scavenging Enzyme Activities in Mice

허근,이상일,송민익,Huh, Keun,Lee, Sang-Il,Song, Min-Ik Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.1

Ethanol을 투여한 mouse 간에서 free radical 생성효소인 xanthine oxidase와 분해효소 활성에 미치는 sinigrin의 영향을 관찰하였다. Ethanol 투여에 의해 간 xanthine oxidase 활성은 대조군에 비해 증가하였으나 $NAD^+$ 의존성 xanthine dehydrogenase 활성은 감소하였다. 한편 sinigrin의 전처리는 ethanol 투여에 의해 증가되는 간 xanthine oxidase의 활성 및 조직 중의 과산화지질 함량을 감소시켰다. Free radical 분해효소인 superoxide dismutase와 catalase 활성을 관찰한 실험에서 sinigrin을 전처리한 실험군은 ethanol 단독 투여군에 비하여 유의적인 효소활성 변동을 나타나지 않았다. The effect of sinigrin was investigated on xanthine oxidase and free radical scavenging enzymes in ethanol-treated mice. In vivo, the treatment of ethanol increased the activity of the $O_2$-dependent hepatic xanthine oxidase compared to those of the control group. Whereas the activity of the $NAD^+$-dependent xanthine dehydrogenase was decreased about 50 percent. The pretreatmemt of sinigrin reduced the increment of the activity of xanthine oxidase and hepatic lipid peroxide content in ethanol-treated mice. On the superoxide scavenging system, the superoxide dismutase activity with the treatment of ethanol showed the trend of increasing, whereas the sinigrin pretreatment did not affect the enzyme activity. The activity of catalase which is another scavenger was not influenced by the ethanol or sinigrin treatment.

Pyrazinamide와 그 대사산물이 유발하는 고뇨산 혈증에 미치는 Pyridoxal의 영향

허근,박종민,신억섭,김석환,Huh, Keun,Park, Jong-Min,Shin, Uk-Seob,Kim, Suk-Hwan 대한약학회 1987 약학회지 Vol.31 No.3

Pyrazinamide, an amide of pyrazinoic acid, is widely used in combination with other drugs for the treatment of tuberculosis. It was attempted to observe the effect of pyridoxal on the pyrazinamide induce hyperuricemia in this study. It was observed that the values of serum transminases were not changed in mice injected pyrazinamide and pyrazinoic acid, respectively (100, 200, and 300mg/kg) compared with control. Blood urate levels were increased in mice treated with these drugs in a dose dependent manner. After pyrazinoic acid was administered intraperitoneally at a dose of 300mg/kg pretreated with 50mg/kg of pyridoxal once daily for 4 days, the blood levels of uric acid and pyrazinoic acid were decreased significantly.

Effect of Panax Ginseng in Mouse Liver with Ethnol-Induced Monoamine Oxidase Activity

허근,이상일,송민익,신억섭,박종민,Huh, Keun,Lee, Sang-Il,Song, Min-Ik,Shin, Uk-Seob,Park, Jong-Min The Korean Society of Pharmacology 1989 대한약리학잡지 Vol.25 No.1

본 실험에서는 ethanol 및 acetaldehyde가 유도하는 MAO 활성변동에 인삼 butanol 분획이 미치는 영향을 검토하였다. Ethanol(1 g/kg)을 mouse 복강내로 투여하였을 때 간장 중 MAO 활성이 현저히 증가하였으며 이러한 작용은 인삼butanol 분획 전처리로 개선되었다. 또한 pyrazole(200mg/kg)을 전처리하였을 때 ethanol에 의해 증가된 간장중 MAO활성이 정상수준으로 감소되었다. 만성적으로 ethanol을 투여한 경우에 있어서도 MAO활성이 증가되었으며 인삼 butaonl분획 전처리로 MAO 활성이 감소됨이 관찰되었다. Ethanol의 대사산물인 acetaldehyde(100mg/kg)을 복강내로 투여하고 관찰한 실험에서도 간장중 MAO활성이 현저하게 증가되었으며 인삼 butanol분획을 전처리함으로써 정상수준 가깝게 MAO활성이 감소되었다. 이 연구결과로 ethanol에 의한 간장중 MAO활성증가는 ethanol의 대사산물인 acetaldehyde에 기인된 것으로 생각되어지며 인삼 butanol분획이 acetaldehyde에 의해 유도되는 간장중 MAO활성 변동을 조절하고 있을 것으로 사료된다. Effect of ginseng butanol fractions on the hepatic mitochondrial monoamine oxidase activity with ethanol treatment was investigated in this experiment. Ethanol treatment, either acutely or chronically, increased the hepatic mitochondrial monoamine oxidase activity compared to control group. Whereas, treatment with ginseng butanol fractions lowered the ethaol-induced monoamine oxidase activity. Acetaldehyde, the major metabolite of ethanol, significantly increased the hepatic mitochondrial monoamine oxidase activity more than ethanol did. It was also observed that ginseng butanol fractions reduced the increase of the hepatic mitochondial monoamine oxidase activity by acetaldehyde. From these results, it is suggested that ginseng butanol fractions may be associated with the modulation of the hepatic mitochondrial monoamine oxidase activity in ethanol-treated animals.

마우스의 아세트알데히드 대사에 미치는 인삼 부탄올 분획의 영향

허근,박종민,이상일,최종원,Huh, Keun,Park, Chong-Min,Lee, Sang-Il,Choi, Chong-Won 대한약학회 1985 약학회지 Vol.29 No.1

The present study was undertaken to investigate the possible effect of ginseng butanol fraction on the hepatic acetaldehyde metabolism. Experimental animals were used for the subject of the study. When, in case of mitochondrial aldehyde dehydrogenase (Ald DH), ginseng butanol fraction was added, enzyme activity was increased in a small dose, while, in a large dose, it showed inhibitory effect. In terms of kinetic aspect, ginseng butanol fraction has the effect to decrease the Km values of Ald DH. In vivo studies, the activity of Aid DH increased by induction of acute intoxication of ethanol was further increased through pretreatment with ginseng butanol fraction. When ginseng butanol fraction was given to mice fed with 5% ethanol instead of water for 60 days, the activity of Ald DH in mitochondrial fraction decreased to about 35% in chronic alcoholism, but after pretreatment of ginseng butanol fraction the activity was restored to the control level. By the pretreatment with disulfiram, the Ald DH activity was inhibited in normal and alcohol-treated groups, but after the treatment with ginseng butanol fraction the activity was restored to the control level. The results suggest that ginseng butanol fraction enhance the Ald DH activity inhibited by the treatment of disulfiram with no relation to NAD. It was observed that ginseng butanol fraction markedly decrease the acetaldehyde levels in plasma and liver. All these observations suggested that reduction of acetaldehyde in blood and liver should be dependent upon increased activity of mitochonclrial Ald DH. It is concluded that the recovery from alcohol intoxication should be prompted by treatment with ginseng.

마늘성분이 간 Xanthine Oxidase 활성변화에 미치는 영향

허근,이상일,박종민 ( Keun Huh,Sang Il Lee,Jong Min Park ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.3

It was attempted to observe the effect of garlic on the hepatic xanthine oxidase activity in this study. Xanthine oxidase which catalyze the oxidation of many purine and pyrimidine derivatives was further purified by centrifugation, heating, (NH₄)₂SO₄fraction, acetone fraction and dialysis, hydroxyapatite column. Allicin and heat-treated allicin fraction powerfully inhibited xanthine oxidase activity in vitro. When the enzyme was incubated in reaction mixture containing garlic butanol fraction, it was found that xanthine oxidase activity was increased. Garlic bulbs and seeds ethanol extracts increased the enzyme activity, whereas leaves and roots extracts did not affected its activity in vitiro.

마늘성분이 간 aldehyde oxidase 활성변화에 미치는 영향

허근,이상일,박종민,김영문,김석환 ( Keun Huh,Sang Il Lee,Young Moon Kim,Jong Min Park Seok Hwan Kim ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.1

Effects of garlic components on the hepatic aldehyde oxidase activity were studied in this experiment. Aldehyde oxidase which catalyzes the oxidation of many purine, pyrimidine and pteridine derivatives was purified by centrifugation, heat treatment, ammonium sulfate fractionation, acetone fractionation and dialysis, and hydroxyapatite column chromatography. Allicin, heat-treated allicin and garlic butanol fraction powerfully inhibited aldehyde oxidase activity in vitro. It was also observed that aldehyde oxidase activity was inhibited in mice fed a 5% garlic juice in vivo.

Sinigrin 이 Mouse 간 Xanthine oxidase 및 Free Radical 분해효소 활성에 미치는 영향

허근,이상일,송민익 ( Keun Huh,Sang Il Lee,Min Ik Song ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.1

The effect of sinigrin was investigated on xanthine oxidase and free radical scavenging enzymes in ethanol-treated mice. In vivo, the treatment of ethanol increased the activity of the O₂-dependent hepatic xanthine oxidase compared to those of the control group. Whereas the activity of the NAD^+ -dependent xanthine dehydrogenase was decreased about 50 percent. The pretreatmemt of sinigrin reduced the increment of the activity of xanthine oxidase and hepatic lipid peroxide content in ethanol-treated mice. On the superoxide scavenging system, the superoxide dismutase activity with the treatment of ethanol showed the trend of increasing, whereas the sinigrin pretreatment did not affect the enzyme activity. The activity of catalase which is another scavenger was not influenced by the ethanol or sinigrin treatment.

Effect of Garlic on the Hepatic Xanthine Oxidase Activity in Rats

허근,이상일,박종민,Huh, Keun,Lee, Sang-Il,Park, Jong-Min 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.3

마늘성분이 간 xanthine oxidase활성변화에 미치는 영향을 검토하였다. rat의 간장에서 본 효소를 분리정제하고 (in vitro에서), 마늘로 부터 추출된 allicin, heat-treated allicin, crude saponin fraction 및 각 부위별 추출물을 시험관내(內)에 첨가하였을 때 아래의 결과를 얻었다. allicin 분획 또는 열처리된 allicine분획을 반응액 중에 첨가하였을 때 강력한 효소 활성 억제효과를 관찰할 수 있었으며 열처리된 allicine분획이 보다 강력한 억제헨상을 나타내었다. 한편, crude saponin fraction은 효소의 활성을 증가시켰다. 식용으로 상용하는 각 부위별 ethanol 추출물에서 뿌리와 잎추출물은 본 효소활성에 별다른 영향을 나타내지 않았으나 seed와 bulb추출물은 효소의 활성을 증가시키는 경향을 나타내었다. It was attempted to observe the effect of garlic on the hepatic xanthine oxidase activity in this study. Xanthine oxidase which catalyze the oxidation of many purine and pyrimidine derivatives was further purified by centrifugation, heating, $(NH_4)_2SO_4$ fraction, acetone fraction and dialysis, hydroxyapatite column. Allicin and heat-treated allicin fraction powerfully inhibited xanthine oxidase activity in vitro. When the enzyme was incubated in reaction mixture containing garlic butanol fraction, it was found that xanthine oxidase activity was increased. Garlic bulbs and seeds ethanol extracts increased the enzyme activity, whereas leaves and roots extracts did not affected its activity in vitiro.