Dexamethasone , Nor - adrenalin 및 ACTH 의 투여가 자성 Rat의 생식기에 미치는 영향

함태수,변명대 ( Tae Su Ham,Myung Dae Byun ) 한국축산학회 1982 한국축산학회지 Vol.24 No.4

The present study was initiated to examine the effects of dexamethasone, nor-adrenalin and ACTH on the reproductive organs of immature female rats. 21day old female rats caged in groups of dexamethasone noradrenalin and ACTH, received oradexon, nor-adrenalin and ACTH subcutaneously daily for 10 days or 21 days, respectively. Rats were sacrificed 24 hr after the last injection. The results obtained in this experiment were as follows: 1. Body weight was significantly (p $lt;0.01) decreased in the dexamethasone-treated rats when compared with the control group. But noradrenalin and ACTH-treated groups were not affected significantly by chronic nor-adrenalin and ACTH treatment for 10 days or 21 days. 2. Mean ovarian weight of immature female rats injected with dexamethasone, nor-adrenalin and ACTH for 10 days were significantly (p $lt; 0.01) reduced as compared to that of control group. The ovarian weight of noradrenalin-treated group increased significantly (p$lt;0.05) after 21 days of treatment with nor-adrenalin. Dexamethasone ACTH-treated group had no significant difference, but they tend to decrease. 3. Uterine weight of female rats decreased significantly (p $lt; 0.01) after injection of dexamethasone for 10 days but noradrenalin treated group receiving nor-adrenalin increased significantly (p $lt; 0.01). Groups injected with dexamethasone, noradrenalin and ACTH for 21 days had no significance but dexamethasone-thasone-treated group tends to decrease in weight of uteri. 4. Adrenal weight of female rats receiving dexamethasone and noradrenalin for 10 days were significantly decreased, while ACTH-treated group were significantly greater (p$lt;0.01) than in rats receiving dexamethasone and nor adrenalin. Dexamethasone group were significantly decreased as compared to that of control group after 21 days of treatment of dexamethasone but ACTH-treated group were significantly increased. Noradrenalin treated group had no significance but it tends to decrease. 5. Compared to those of controls, Liver were 69.8 % lighter in 31-day old females, while spleens were 185.3% heavier. The weights of the liver and spleen were reduced by 64.5 % and 27.2 % respectively in 42-day old female. Kidney and heart weights were not affected significantly by chronic dexamethasone treatment. 6. In histological findings of rat ovaries treated with dexamethasone, granulosa cells had necrosis and the majority of follicles showed varying degrees of atresia. 7. Histological appearances of the ovaries showed numerous follicles after 10 days of treatment of nor-adrealin. Luteal cells showed proliferation of fibroblast and vacuolization after 21 days of treatment. Histological studies indicated that corpora lutea showed retrogressive changes. 8. In the overies of ACTH-treated rats for 10 days, granulosa cells of follicles were ruptured and follicular atresia were increased. Luteolysis was observed in rat ovaries treated with ACTH for 21 days but was not remarked as compared to that of noradrenalin treated group.

The regional distribution and relative frequency of gastrointestinal endocrine cells of the ICR mice: An immunohistochemical study

함태수,Ham, Tae-su The Korean Society of Veterinary Science 2001 大韓獸醫學會誌 Vol.41 No.2

ICR 마우스 위장관 8개 부위(위저부, 유문부, 십이지장, 공장, 회장, 맹장, 결장 및 직장)에서 위장관내분비 세포의 부위별 분포 및 상대적 빈도를 somatostain, serotonin, glucagon, chloecystokinin(CCK)-8, secretin, pancreatic polypeptide(PP) 및 gastrin 등 총 7종류의 항혈청을 이용한 면역조직화학적 방법으로 관찰하였던 결과 somatostain, serotonin, glucagon, CCK-8, secretin, 및 gastrin 면역반응세포의 7종류의 내분비세포가 관찰되었다. 본 실험의 결과 장관부위에서는 주로 타원형 또는 방추형의 개방형 세포(open-typed cell)들이 관찰된 반면 위저부와 유문부에서는 주로 원형의 폐쇄형 세포(close-typed cell)을이 관찰되었다. 이들 면역반응세포들의 부위별 분포는 위장관 각 부위에 따라 매우 다양하게 관찰되었다. Somatostain 면역반응세포들은 대장을 제외한 위장관에서 전 부위에서 관찰되었고, serotonin 면역반응세포들은 전 위장관에 걸쳐 관찰되었으며, ICR 마우스에서 가장 높은 빈도를 나타내었다. Glucagon 면역반응세포들은 위저부와 직장에 국한되어 관찰되었으며, 각각 중등도 및 소수의 빈도를 나타내었다. CCK-8 면역반응세포들은 유문부, 십이지장 및 회장에서 각각 다수, 중등도 및 극소수의 빈도로 관찰되었다. 한편 secretin 면역반응세포들은 각각 소수 및 극소수의 빈도로 십이지장과 회장에 국한되어 출현하였고, gastrin 면역반응세포들은 유문부에 국한되어 다수 관찰되었다. 그러나 PP 면역반응세포들은 전 위장관에 걸쳐 관찰되지 않았다. 결론적으로 ICR 마우스의 위장관내분비세포의 부위별 분포 및 상대적 빈도는 다른 포유동물과 유사하게 관찰되었으나, 일부 특이한 양상을 나타내기도 하였다. The regional distributions and relative frequencies of some gastrointestinal endocrine cells in the 8 portions (fundus, pylorus, duodenum, jejunum, ileum, cecum, colon and rectum) of the gastrointestinal tract of ICR mouse (ICR) with immunohistochemical method using 7 types of specific antisera against somatostatin, serotonin, glucagon, cholecystokinin (CCK)-8, secretin, pancreatic polypeptide (PP) and gastrin. In this study, somatostatin-, serotonin-, glucagon-, CCK-8-, secretin- and gastrin-immunoreactive (IR) cells were identified. Most of these IR cells in the intestinal portion were generally spherical or spindle in shape (open-typed cell) while cells showing round in shape (close-typed cell) were found in the stomach regions occasionally. Their relative frequencies were varied according to each portion of gastrointestinal tract. Somatostatin-IR cells were demonstrated throughout whole gastrointestinal tract except for large intestine. Serotonin-IR cells were detected throughout whole gastrointestinal tract and they were most predominant endocrine cell types in this species of mouse. Glucagon-IR cells were restricted to the fundus and rectum with moderate and a few frequencies, respectively. CCK-8-IR cells were observed in the pylorus, duodenum and ileum with numerous, moderate and rare frequencies, respectively. Secretin-IR cells were restricted to the duodenum and ileum with a few and rare frequencies, respectively. Gastrin-IR cells were restricted to the pylorus with numerous frequency. However, no PP-IR cells were found in this study. In conclusion, some peculiar distributional patterns of gastrointestinal endocrine cells were found in the ICR mouse compared to those of other mammals.

Immunohistochemical study of pancreatic poly-peptide-immunoreactive cells in the pancreas of the Korean native goat (Capra hircus) during developmental stages

함태수,Ham, Tae-su The Korean Society of Veterinary Science 2000 大韓獸醫學會誌 Vol.40 No.3

태아기, 신생아기, 생후 1개월령, 6개월령 및 성체의 한국재래산양 췌장에서 pancreatic poluypeptide(PP) 면역반응세포의 분포 및 출현빈도를 PAP 법에 의해 검색하였다. 외분비부에서는 모든 연령에서 관찰되었으며 췌장세포 사이에 분포하였다. PP 면역반응세포는 신생아가기에서 생후 1개월에 증가한 후 점차 감소하였다. 췌도관에서는 PP 면역반응세포가 생후 1개월부터 관찰되면 그후 점차 감소하였다. 췌도관에서는 PP 면역반응세포가 생후 1개월부터 관찰되면 그후 점차 감소하였다. 이들 면역반응세포는 생후 1개월령 및 6개월령의 췌도관 상피하 결합조직에서 소수 관찰되었다. 그러나 성체에서는 췌도관 상피세포 사이에서 극소수 관찰되었다. 그러나 성체에서는 췌도관 상피세포 사이에서 극소수 관찰되었다. 내분비부(췌장섬)에서 PP 면역반응세포는 신생아기부터 관찰되며, 생후 1개월령부터의 PP면역세포의 분포는 췌장성 주변부에 중등도 또는 소수가 산재하는 것과 다수의 PP 면역반응세포가 췌도 전체 (생후 1개월령)또는 췌도 중앙부(생후 6개월령)에 산재하는 것 등 두가지 유형을 나타내었다. 결론적으로 한국 재래산양의 췌장에 있어서 PP 면역반응세포의 분포와 출현빈도는 발생단계에 따라 상이하였다. 이는 발생단계 동안의 식이성 및 생리학적 조건변화가 이와같은 상이함을 유발하는 요인이 된 것으로 사료된다. The regional distribution and relative frequency of the pancreatic polypeptide (PP)-immunoreactive cells in the pancreas of the Korean native goat (Capra hircus) were studied by immunohistochemical methods (PAP methods) using specific antisera against to PP during developmental stage, fetus, neonate, 1-month-old, 6-month-old and adult. The different regional distribution and relative frequency of PP-immunoreactive cells in the pancreas of Korean native goat were observed during development. In the exocrine portions, they were detected in the all ages and distributed in the interacinar regions. PP-immunoreactive cells were increased from neonate to 1-month-old but thereafter decreased with developmental stage. In the pancreatic duct, PP-immunoreactive cells were demonstrated from 1-month-old but they were decreased with developmental stages in these regions. These cells were observed in the subepithelial connective tissue of the pancreatic duct with a few frequencies in 1-and 6-month-old. However, they were located in the interepithelial cells of the pancreatic duct in the adult with rare frequency. In the endocrine portion (pancreatic islets), PP-immunoreactive cells were observed from neonate, and the regional distribution of PP-immunoreactive cells from 1-month-old was divided into two patterns : dispersed 1) in the marginal regions of the pancreatic islets with moderate or a few frequencies, and 2) in the whole pancreatic islets (in a case of 1-month-old) or in the central cores (in a case of 6-month-old and adult) with numerous frequency. In conclusion, the different regional distribution and relative frequency of PP-immunoreactive cells in the pancreas of the Korean native goat were observed during development. It is suggested that the changing of feeding habits and physiological conditions during different developmental stages may cause these differences.

An immunohistochemical study of the serotonin-immunoreactive cells in the developing pancreas of the chicken embryos

함태수,Ham, The-su The Korean Society of Veterinary Science 2001 大韓獸醫學會誌 Vol.41 No.2

부란 1일부터 부화직후까지의 닭 태자 췌장에서 serotonin 면역반응세포들의 부위별 분포 및 상대적 빈도를 면역조직화학적 방법으로 검색하였다. 췌장은 해부학적으로 배쪽, 등쪽, 제 3엽 및 비장엽의 4개엽으로 구분하였으며, 각 엽은 조직학적으로 외분비 부분, light 및 dark 췌장섬의 3부분으로 세분하였다. 이들의 각 발생단계에 따른 닭 췌장에서 serotonin 면역반응세포들의 분포 및 빈도는 췌장의 엽, 조직학적 부위 및 발생단계에 따라서 매우 다양하게 관찰되었으나, 대체로 원형 또는 난원형의 형태로 모든 엽에서 관찰되었다. 외분비 부분에서 serotonin 면역반응세포들은 비장엽의 경우 부란 13일과 14일에서만 국한되어 관찰되었고, 제 3엽에서는 부란 10일부터 부란 19일 동안 관찰되었다. 또한 배쪽엽에서는 부란 10일부터 부화 직후까지 관찰되었으며, 등쪽엽에서는 부란 11일부터 부화 직후까지 관찰되었다. 췌장섬에서 이들 면역반응세포는 비장엽의 dark 췌장섬에서만 부란 15일과 부란 16일에 국한되어 극소수 관찰되었고 다른 엽 또는 light 췌장섬에서는 관찰되지 않았다. 결론적으로 serotonin 면역반응세포들은 부란 발생 초기에는 다수 관찰된 이후 발생단계에 따라 점차적으로 감소되며 이런 양상은 엽의 종류에 관계없이 나타나는 것으로 관찰되었다. The distributions and relative frequencies of the serotonin-immunoreactive cells were studied in dorsal, ventral, third and splenic lobes of developing chicken pancreas during embryonic periods (10 days of incubation to hatching) by immunohistochemical methods. The regions of pancreas were subdivided into three regions, exocrine, light and dark islets. Round and/or oval shaped serotonin-immunoreactive cells were detected in all four lobes. According to developmental stages, the types of lobes and the regions of pancreas, these immunoreactive cells were showed various distributions and relative frequencies. In exocrine portions, serotonin-immunoreactive cells were found in the splenic lobes at 13-14 days of incubation, in the third lobes from 10 days to 19 days of incubation, in the ventral lobes from 10 days of incubation to hatching and in the dorsal lobes from 11 days of incubation to hatching. In pancreatic islets, these cells were detected only in the dark islets of splenic lobes at 15 and 16 day of incubation with rare frequency. In conclusion, serotonin-immunoreactive cells decreased with developmental stages in all four lobes and their relative frequencies decreased with developmental stages.

Prostaglandin F2α의 투여가 웅서의 혈중 Testosterone 과 LH 함량 및 부생식기에 미치는 영향

함태수 ( T . S . Ham ) 한국축산학회 1986 한국축산학회지 Vol.28 No.12

The present study was undertaken to examine the plasma testosterone concentration of male rats treated with prostaglandin F₂α. In addition, the effects of prostaglandin F₂α on plasma levels of luteinizing hormone (LH), and on the weights of seminal vesicles, ventral prostate and testes were examine in male rats. Rats was injected with 250 ㎍ and 1,000 ㎍ of PGF₂α single for 8 days. The concentrations of testosterone (T) and LH were determined by radioimmunoassay (RIA) after the 8 days treatment period. The results in this experiment were as follows; 1. The concentration of testosterone in plasma of rats treated with 1,000㎍ was 2.73±0.40 ng/㎖. at 14h and 0.92±0.02 ng/㎖ compared to 3.90±0.41 ng/㎖ in control animals and the posttreatment levels were significantly lower than those of pretreatment (p$lt;0.01) at 17 h. However, PGF₂α (250 ng/rat) had no significant effect on plasma testosterone in rats treated with 250 ㎍/day for 8 days. 2. Plasma LH concentration was slightly decreased at 14h but returned to the initial level at 17h. The plasma LH concentration was not affected by the injection of 250 ㎍ or 1,000 ㎍/rat of PGF₂α. 3. Testis weight after treatment with 250 ㎍ and 1,000 ㎍ of PGF₂α was not significant. However, the weights of accessory reproductive glands were greater than in control rats.

Regional Distribution and Relative Frequency of the Neurotensin-immunoreactive Cells in the Alimentary Tract of the Red-eared Slider, Trachemys scripta elegans

함태수(Tae-su Ham) 한국실험동물학회 2000 Laboratory Animal Research Vol.16 No.2

'81 , '82 년도 연구소원 본지 게재외 논문초록 : Dexamethasone , Noradrenalin 및 ACTH 의 투흥가 수성 Rat의 생치계에 미치는 영향

변명태,함태수(Tae Su Ham) 영남대학교 자원문제연구소 1983 資源問題硏究 Vol.2 No.-

한국재래산양의 도체구성에 관한 연구

서영석,함태수 ( Y . S . Sye,T . S . Ham ) 한국축산학회 1998 한국축산학회지 Vol.40 No.3

This experiment was carried out to study the carcass composition between two kinds of goat, fattening Korean Native Goat and 3 years old goat raised in the farm. The average dressing percent of fattening herd was 47.5% while that of 3 years old herd was 45.5%. The neck portion percent in carcass weight was 13.4% in 3 years old herd and this was higher than that of the fattening herd, 11.1%(P$lt;0.01). The forequarter percentages were 50.0% in the fattening herd and 51.4% in 3 years old herd. The hindquarter percentage of fattening herd was 38.9% which was higher than that of the 3 years herd, 35.2%(P$lt;0.05). Edible meat percent in carcass of the 3 years herd was 69.6% which was higher than that of the fattening herd, 63.9%(P$lt;0.05). The fat percent of fattening was 16.6%. It was almost twice compared to that of 3 years old herd, 8.6%(P$lt;0.01). The bone percent of the fattening herd was 18.3% while that of the 3 years herd was 20.0%. The highest meat portion was found in the neck part of the 3 years herd as 73.9%. The highest fat content was 20.0% in the forequarter of the fattening head. The meat portion of slaughter weights of fattening and 3 years old herds were 29.1% and 32.0%, respectably(P$gt;0.05).

도축돈에서 Pasteurella multocida의 분리, 분리주에 대한 미생물학적, 혈청학적성상 및 항생물질의 감수성조사와 돈폐에 대한 병리학적 관찰에 관하여

이학철,함태수,정유열,조성룡,이재현,Lee Hak-Cheol,Ham Tai-Soo,Chung Yoo-Yeol,Cho Seong-Lyong,Lee Jag-Hyeon 한국임상수의학회 1989 한국임상수의학회지 Vol.6 No.2

In recent years much attention has been paid to swine respiratory infection caused by Pasteurella(P) multocida with rapid expansion of pork Industry in Korea. The present study was performed to observe the etiologic situation of P. multocida infection by bacteriological, serological(serotyping) and pathological examinations with the lungs respectively. In addition antibiotic susceptibility test was carried out against the isolated strains of P. multocida. The results obtained are as follows : 1. Eighteen strains(12.8%) wert isolated from the 140 cases of swine lungs examined, and biological and biochemical characteristics of the isolates were the sam as those in the references of other workers, whereas some differences were observed in sugar fermentation and enzyme activity according to the strain of isolates. 2. Capsular serotyping performed on 18 P. multocida revealed that 13 strains(72.2%) were A type and 5 strains(27.8%) were D type, respectively. 3. When serotyping was performed against somatic antigen on 18 strains capsular types of which were identified as described above 9(50%), 3(16.7%) and 4(22.2%) strains belong to 1:A, 3:A and 2:D, respectively, but untypable 2 strains(11.1%) were observed. 4. Antibiotic susceptibility test by employing disc method for 24 kinds of drugs revealed that 15 kinds of antibiotics were sensitive to 18 strains of P. multocida isolated such as ampicillin(l00%), penicillin(100%), cloxacillin(56%), piperacillin(70%), cefotaxime(30%), minocycline(60%), chloramphenicol(95%), erythromycin(39%), kanamycin(17%), gentamicin(70%), amikasin(30%), colistin(78%) and nalidixic acid(5%), respectively, but resistant to 9 kinds of antibiotics such as sulpenicillin, cefazolin, cephalothin, cefametazol, cefoperazone, kitasamycin, oleandomycin. lincomycin and bacitracin. 5. Pathological features of 60 cases of swine lungs indicated that pneumonic .lesions were observed in 38 cases(63.3%) examined by macroscopic finding, in which lesions of 8 cases(13.4%) would correspond to those of mycoplasmal infection, and 30 cases(50%) were similar to viral infection by histopathological finding, whereas 22 cases(36.7%) were considered to be normal by ecropsy or histopathological finding.

Serranidae (Coreoperca herzi) 췌장 내분비세포에 대한 면역조직화학적 연구

이재현,구세광,이형식,함태수,Lee, Jae-hyun,Ku, Sae-kwang,Lee, Hyeung-sik,Ham, Tae-su The Korean Society of Veterinary Science 2003 大韓獸醫學會誌 Vol.43 No.3

The regional distribution and relative frequency of some endocrine cells in the pancreas of the Korean aucha perch, Coreoperca herzi Herzenstein belonging to the family Serranidae in order Perciformis, were observed using specific mammalian antisera against serotonin, insulin, glucagon, somatostatin and human pancreatic polypeptide (hPP) by peroxidase antiperoxidase (PAP) method. The pancreas was divided into four portions (principal and secondary islets, exocrine and pancreatic duct regions). In addition, the pancreatic islet regions were further subdivided into three regions (central, mantle and peripheral regions). Spherical to spindle or occasionally round to oval immunoreactive (IR) cells were demonstrated in the pancreatic islets and exoccrine portions, but no cells were detected in the pancreatic duct portions. In the principal islets, serotonin-IR cells were not detected but most of insulin-IR cells were located in the central regions and they were also demonstrated in the mantle and peripheral regions in moderate and rare frequencies, respectively. Glucagon- and hPP-IR cells were mainly situated in the mantle regions but the cells were also demonstrated in the peripheral regions in relatively lower frequency. Somatostatin-IR cells were evenly distributed in the central and mantle regions in a few frequency and cells were also demonstrated in the peripheral regions in rare frequency. Cell clusters were consisted of hPP-IR cells that were situated in the peripheral to mantle regions. In the secondary islet portions, serotonin-IR cells were randomly distributed throughout the whole pancreatic islet regions but lower frequency was detected in the peripheral regions compared to that in central and mantle regions where cells were detected in a few frequency, respectively. Insulin-IR cells were restricted to the central regions in numerous frequency and glucagon-IR cells were evenly distributed in the mantle and peripheral regions in moderate frequencies, respectively. Somatostatin-IR cells were observed in the central and mantle regions in moderate and a few frequencies, respectively. In addition, hPP-IR cells showed similar distributional patterns to those of glucagon-IR cells except cells were also located in the central regions in rare frequency. In the exocrine portions, only glucagon- and hPP-IR cells were demonstrated in rare and a few frequencies, respectively. In conclusion, the regional distribution and relative frequency of pancreatic endocrine cells of the Korean aucha perch showed general patterns, which were observed in other teleost. However, some species-dependent different distributional patterns and/or relative frequencies were also demonstrated especially to serotonin-IR cells. In pancreas of the Korean aucha perch, insulin-IR cells were the most predominant cell type followed by glucagon-, somatostatin-, hPP- and serotonin-IR cells.