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상륙에서 추출한 ${\alpha}-spinasterol$의 백혈병세포주(U937) 자멸사 유도 효능
양준석,정상훈,김호,한웅,진재호,정일국,김대근,정승일,정한솔,이광규,Yang, Jun-Seok,Jeong, Sang-Hun,Kim, Ho,Han, Ung,Jin, Jae-Ho,Jung, Il-Kook,Kim, Dae-Keun,Jeong, Seung-Il,Jeong, Han-Sol,Lee, Kwang-Gyu 대한동의생리학회 2007 동의생리병리학회지 Vol.21 No.5
To investigate the possible mechanism of ${\alpha}-spinasterol$ as a candidate of anti-cancer drug, I examined the effects of ${\alpha}-spinasterol$ on the apoptosis of U937 cells MTT assay, flow cytometric analysis, SDS-polyacrylamide gel electrophoresis, Western blot analysis, and RT-PCR were performed. ${\alpha}-spinasterol$ treatment reduced the cell viablilty of U937 cells in a dose-dependent manner, which was associated with the induction of apoptotic cell death. ${\alpha}-spinasterol$ treatment also reduced the levels of Bcl-xL anti-apoptotic protein expression and increased the levels of caspase-3, p53, pro-apoptotic protein, in U937 cells. After treatment the level of Bcl-xL, anti-apoptotic gene expression was decreased and the level of ICE pro-apoptotic gene expression was increased. These findings suggest that ${\alpha}-spinasterol$ induced the apoptotic cell death via regulation of several growth regulatory gene products. The abbreviations used are: FBS, fetal bovine serum; PBS, phosphate buffered saline; PI, propidium iodide; OD, optical density; DiOC6, 3,3-dihexyloxa carbcyanine iodide; MTT, 3 [4-5-dimethylthiazol-2-yl] -2-diphenyltetrazolium bromide.
U-937 세포에서 도적승기탕(導赤承氣湯) 추출물 중 부탄올 분획에 의한 Apoptosis 유도
박평범,정한솔,김호,진재호,정상훈,한웅,이문원,이광규,Park, Pyeong-Beom,Jeong, Han-Sol,Kim, Ho,Jin, Jae-Ho,Jeong, Sang-Hun,Han, Ung,Lee, Moon-Won,Lee, Kwang-Gyu 대한동의생리학회 2006 동의생리병리학회지 Vol.20 No.6
To investigate the anti-cancer effects of n-butanol fraction of DoJeokSeungKi-Tang extracts(nBFD) in U-937 cells. MTT assay was used to determine U-937 cells proliferation. Flow cytometry was used to detect apoptosis. Bcl-xl anti-apoptotic protein and caspase-3, p53 pro-apoptotic protein were examined by Western blot analysis. nBFD inhibited the proliferation of U-937 cells in a dose-dependent manner. The cells treated with nBFD showed a typical apoptotic process by increasing sub-Gl peak. nBFD reduced uptake of 3,3'dihexyloxacarbocyanine iodide(DiOC6) a fluorochrome which incorporates into cells dependent upon their mitochondrial transmembrane potential$({\triangle}{\psi}m)$. nBFD induced in U-937 cells apoptosis mainly via increasing sub-Gl peak, regulation of Bcl-xl, caspase-3 and p53 protein.