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천연과즙을 이용한 Aloe vera L.의 callus 배양과 이들 추출물의 항산화 및 항균활성 효과 검정
이인순(In-Soon Lee),배동녁(Dong Nyeok Bae),권오민(Oh Min Kwon),한구태(Gu Tai Han),김대환(Dae Hwan Kim),오명원(Myeong Won Oh),이지홍(Ji Hong Lee),문혜원(Hae-Yeon Moon) 한국생물공학회 2013 KSBB Journal Vol.28 No.6
In order to obtain functional materials from aloe callus, we cultured Aloe vera L. leaf on MS medium added 0.2 mg/L IAA, 0.3 mg/L kinetin and 100 mg/L grape or/and apple juice for 30 days. While a callus differentiation during callus culture did not show, the cultured leaves were uniquely released extracellular material into the agar plate. After cultivation for 18 days, the cultured leaf and agar were harvested for extraction a functional material. The materials extracted were measured on the amount of total phenols, flavonoids and polysaccharides and determined on the antioxidant and antimicrobial activity. In result, callus extracts of additive free (CT) and added apple juice (2T) had more amount of phenol compound (659 μg/mL, 533 μg/mL) and flavonoid (580 μg/ mL, 501 μg/mL) than natural leaf (p: 525 μg/mL, f: 301 μg/ mL). However, the extract of natural leaf had the better effect of lipid peroxidation and polysaccharide content than the culture extract. All samples extracted had same effect on the nitrite scavenging activity. On the other hand, only 2T extract showed excellent 72% nitric oxide scavenging activity. The agar extract was also confirmed to contain polyphenol compound and polysaccharide content that had antioxidant and antimicrobial activity partly.