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현방훈,김인중,표현미,차상호,박지연,송재영,조인수,양창범,안수환,이중복,Hyun, Bang-Hun,Kim, In-Joong,Pyo, Hyun-Mi,Cha, Sang-Ho,Park, Ji-Yeun,Song, Jae-Young,Cho, In-Soo,Yang, Chang-Bum,An, Soo-Hwan,Lee, Joong-Bok 대한수의학회 2009 大韓獸醫學會誌 Vol.49 No.1
The molecular genetic characterization of Aujeszky's disease virus (ADV) Yangsan strain (ADVYS), a Korean isolate, was investigated by analyzing the electrophoresis patterns and the physical maps of the viral DNA digested with various endonucleases. To establish DNA library for ADV-YS, twelve major BamHI restricted segments were cloned. Each location of the segments in the ADV genome was determined by sequence comparison with the sequences reported in Genbank and those sequences of the both termini of the segments. Physical maps were constructed based on the electrophoresis patterns of the digested viral DNA by restriction endonuclease and the results of Southern blot analyses with various DIG labeled probes originated from those of enzyme restricted segments of virulent (Shope) and avirulent (Bartha) strain. Comparing ADV-YS with a standard strain of Kaplan in the maps of restriction enzymes, following major respects were identified: (i) disappearance of BamHI restriction site between the first and second BamHI segments, (ii) creation of the BamHI restriction site in the fifth segment, and (iii) generation of the BglII site in the unique short (US) region. The genome of ADV-YS also contains a type 2 herpesvirus DNA molecule (in which the US region only inverts itself relative to the unique longregion) like all other ADV strains except Norden strain(type3), analyzed up to date. The size of the ADV genome estimated from the sizes of the restriction enzyme fragments, was approximately 145.3 kb (BamHI) or 145.4 kb (BglII). BamHI enzyme cleavage patterns were compared among the five Korean ADV isolates: Yangsan, Yongin, Dangjin, Jincheon and Iksan strains. Difference either in the number or in the size of the DNA fragments, suspected regions of termini of IR and TR, could be detected among all five strains.
박민수(Min-Soo Park),표현미(Hyun-Mi Pyo),손종렬(Jong-Ryeul Sohn),변상훈(Sang-Hoon Byeon) 한국실내환경학회 2008 한국실내환경학회지 Vol.5 No.3
According to the measurements at Seoul Metro subway, the sound level at the time of subway arrival ranged from 77.0 to 92.5 ㏈(A). The highest level was 92.5 ㏈(A) at Line-1, and the lowest level was 77.0 ㏈(A) at Line-5. The sound level of departure ranged from the lowest level of 82.9 ㏈(A) at Line-5 and the highest level of 95 ㏈(A) at Line-1. The indoor sound level at subway ranged from the lowest level of 87.2 ㏈(A) (Line-6) to the highest value of 92.1 ㏈(A) (Line-1). The results of this study also showed that the sound level at subway platform with Platform Screen Door(PSD) showed 81.8 ㏈(A) but 89.1 ㏈(A) without PSD. The sound reduction effect of PSD showed 7.3 ㏈(A) at platform. Consequently, the sound level in Korean subway was found to exceed the standard set for the American Public Transportation Association (85㏈(A)).
국내분리 돼지 뇌심근염바이러스의 poly(C)-tract의 다형태성
현방훈,김효진,김인중,표현미,김선미,김성희,김재조,임성인,송재영,Hyun, Bang-Hun,Kim, Hyo-Jin,Kim, In-Joong,Pyo, Hyun-Mi,Kim, Sun-Mi,Kim, Seong-Hee,Kim, Jae-Jo,Lim, Seong-In,Song, Jae-Young 대한수의학회 2010 大韓獸醫學會誌 Vol.50 No.3
Encephalomyocarditis virus (EMCV) belongs to the genus Cardiovirus within the family Picornaviridae. EMCV has been recognized either as a cause of mortality in young pigs, due to acute myocarditis, or of reproductive failure in sows. An EMCV K3 strain was isolated from the heart and brain in a mummified and aborted swine fetus in 1989. For the molecular characterization of the poly(C)-tract of EMCV Korean isolates, K3 strain, viral RNA was extracted and digested with RNase T1, and analyzed the length of the poly(C)-tract by polyacrylamide gel electrophoresis. The poly(C) regions also were amplified by RT-PCR and sequenced. The present study shows that K3 strain of EMCV had a short polymorphic poly(C) tracts (5 to 30 C's) with sequences consisting of $C_9$, $C_{10}$, $C_{13}$, $C_{14}$, $C_{16}$, $C_{20}$, $CUC_{11}$, $C_8UCUC_3UC_{10}$, $C_9UCUC_3UC_{10}$, $C_{10}UCUC_3UC_{10}$, etc. These polymorphism of poly(C)-tracts of EMCV K3 strain implies the historical information of in vivo and/or in vitro passage.