Polymerase Chain Reaction 을 이용한 Apolipoprotein E 유전자의 Genotyping

팽정령,김덕윤,우정택,김성문,양인명,김진우,김영설,김광원,최영길 한국지질학회 1991 韓國脂質學會誌 Vol.1 No.1

Polymerase Chain Reaction 을 이용한 Apolipoprotein E 유전자의 Genotyping

팽정령,김덕윤,우정택,김성운,양인명,김진우,김영설,김광원,최영길 한국지질동맥경화학회 1991 韓國脂質學會誌 Vol.1 No.1

제2형 당뇨병에서 Apolipoprotein C-Ⅱ 유전자의 제한분절길이 다양성

팽정령,김성운,양인명,김진우,김영설,김광원,최영길 대한내분비학회 1990 Endocrinology and metabolism Vol.5 No.부록2호

사람 적혈구에서 인슐린 수용체의 정제 및 결합특성에 대한 연구

팽정령 ( Jung Ryung Paeng ),정수경 ( Soo Kyung Jung ),서광식 ( Kwang Sik Suh ),이군자 ( Gun Ja Lee ) 대한임상검사과학회 1986 대한임상검사과학회지(KJCLS) Vol.18 No.1

당뇨병 환자에서의 PCR를 이용한 apolipoprotein E 유전자형 분석

팽정령 ( Jung Ryung Paeng ),김덕윤 ( Deog Yoon Kim ),우정택 ( Jung Taek Woo ),김성운 ( Sung Woon Kim ),양인명 ( In Myung Yang ),김진우 ( Jin Woo Kim ),김영설 ( Young Seol Kim ),김광원 ( Kwang Won Kim ),최영길 ( Yong Kil Choi ) 대한임상검사과학회 1992 대한임상검사과학회지(KJCLS) Vol.24 No.1

당뇨병은 고지혈증이 흔히 동반되며 다양한 벼인이 관여된다. Apolipoprotein E(단백질의 변이는) 고지혈증 유발성과 apo E isoform 의 분석이 시행되고있다. 최근 apo E 유전자형을 밝히는데 polymerase chain reaction(PCR) 방볍이 이용되고 있다. Apo E 는 299개의 아미노상으로 되어 있고 3개의 isoform 을 갖고 있는데 112번 158번째 아미노산서열의 차이점에 따라 E2, E3, E4로 구분한다. 본 연구에서는 apo E gene 을 PCR 법으로 증폭시켜 Hha I 제한효소로 소화시킨 다음 polyacryl-amide gel electrophoresis 하여 간편하고 신속하게 유전자형을 결정하였다. Hha I 은 Arginine 을 encod-ing 하는 GCGC sote를 인식 절단하는 효소이며 112arg(E4)과 158 arg(E3, E4)는 전단하지만 112cys(E2, E3) 와 15CYS(E2)는 GTGC 로 절단하지 못한다. 이러한 제한효소의 특성을 이용하여 Hha I restriciction fragment length 를 조사하였다. 당뇨병 환자 79명에서 각가의 유전자형을 분류한 결과 E2(6.3%), E3(81.0%), E4(12.7%)로 나타났으며 정신인 73명에서의 유전자형은 E2(4.8%), E3(84.9%), E4(10.3%)로 나타났다. 이상의 결과에서 보면 당뇨병 환자와 대조군과의 유전자 빈도에서의 통계적 유의성은 없었다. 그러나 유전자 빈도에 있어서는 우리 결과와 일본의 결과는 매우 유사하지만 유럽 지역의 결과와는 차이를 보였다. 이러한 결과의 차이는 인종간의 차이 및 유전자형을 결정한는 PCR법과 단백질을 분석하는 isoelectric focusing 간의 차이로 생각되며 향후 PCR법 이 유전자형을 결정하고 진단하는데 중요한 분야기 될 것으로 사료된다.

Graves병 환자의 HLA-DQ β 유전자의 제한효소 분절 길이의 다형성 : Restriction Fragment Length Polymorphism, RFLP

양인명,팽정령,김성운,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1990 遺傳工學論文集 Vol.2 No.-

The distributions of HLA-DR and DQ antigen were compared between 40 patients with, Graves' disease and 24 normal controls. The frequency of HLA-DR 7 among 16 types of HLA-DR was significantly lower in the patient group. And the frequency of HLA-DQw4 among 6 types of HLA -DQ was significantly higher in the patients group. We compared the restriction fragments of HLA-DQ β DNA digested with Bam HI and Taq I and hybridized with DQ β cDNA between 10 patients and 10 normal controls who were homozygotes of HLA-DQw1 or DQw6. There was no band showing significant difference in the frequency. These data suggest that the pattern of linkage between HLA class Ⅱ antigens and the disease susceptibility in Korean is different from that in Caucacian, and a gene responsible for disease suceptibility is not located on DQ βgene (J Kor Soc Endocrinol 5:29-36, 1990).

당뇨병 백서의 간세포에서 Glucokinase 활성도 및 유전자 발현에 대한 인슐린의 영향

강성이,팽정령,서광식,안규정,우정택,김성운,양인명,김진우,김영설,김광원,최영길 경희대학교 유전공학연구소 1993 遺傳工學論文集 Vol.5 No.-

목적 당대사의 조절 상태에 따른 생체 변화를 분자 수준에서 이해하고자 식이 조건을 달리한 정상 백서와 화학적으로 유도된 당뇨병 백서의 간조직에서 혈당수준과 인슐린치료 정도에 따라 나타나는 글루코키나제 활성도 및 유전자 발현을 분석하였다. 방법 스트렙토조토신 정맥투여 후 당뇨병의 유발을 확인하고, 인슐린을 1일 3회 3일간 복강내로 투여하여 상태를 안정시킨 후, 인슐린 투여군은 인슐린 투여 6시간이내에 그리고 인슐린 비투여군은 24시간 후 단두하여 채혈하고 복강을 열어 간조직을 채취하였다. 채취한 간조직에서 글루코키나제 활성도는 인산화된 포도당에서 NADH의 형성을 형광분광계로 측정하였으며, 글루코키나제 유전자 mRNA발현은 Northern 분석법을 이용하였다. 성적 정상 백서에서 공복상태와 식이를 섭취한 경우에 간조직의 글루코키나제 효소의 활성은 차이가 없었으나, 글루코키나제 유전자 mRNA 발현은 증가되었다. 당뇨병이 유발된 백서의 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현은 정상 백서에 비하여 낮았다. 인슐린 투쳐 후 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA 발현이 증가되었고, 특히 혈당이 정상화된 경우에서 글루코키나제 유전자의 mRNA 발현이 증가도었다. 결론 인슐린에 의한 간조직에서 글루코키나제 효소의 활성 및 글루코키나제 유전자의 mRNA을 증가를 볼수 있었다. 당뇨병 백서에서 인슐린 투여 후에 혈당조절이 안된 경우 간조직의 글루코키나제 유전자의 mRNA 발현이 증가가 없는 것으로 보아 글루코키나제 mRNA의 발현에는 인슐린 이외의 다른 요소가 관여할 것으로 생각된다. The liver-specific hexokinase isoenzyme, referred to as glucokinase, is thought to play a key reglulatory role in hepatic glucose metabolism. The glucokinase gene is, therefore, of interest both because of its tissue-specific expression and because of the several regulatory processes that can be analyzed. The level of hepatic glucokinase activity appears to be determined essentially by regulation of the rate of enzyme synthesis, with insulin playing a leading role as an inducer. We investigated the role of insulin for the induction of glucokinase in the liver of diabetic rats. Experimental diabetes was induced by injection of streptozotocin 7 days before the experiment. Regular insulin was given by three days intraperitoneal injection at 8-h interval. The glucokinase mRNA in the liver was estimated by Nothern blot assay, as well as by fluorometric enzyme activity assay. Glucokinase activity was not reduced in the liver of normal fasting rats as compared to normal fed rats. And glucokinase activity was reduced in the liver of diabetic rats as compared to normal rats. In diabetic rats treated with insulin, glucokinase enzyme activity were increased. But glucokinase mRNA expression was only increased in normoglycemic diabetic rat with treated with insulin as compared to hyperglycemic rat. These data indicate that insulin stimulates hepatic glucokinase enzyme activity and mRNA expression. But other hormonal or metabolic factors may be contribute to regulation of glucokinase mRNA expression.

당뇨병, 뇌졸중 및 급성심근경색증에서의 아포리포단백질 E의 유전자형 분포

김진우,팽정령,최영길,김영설,김광원,양인명,김성운,우정택,강성이 대한내분비학회 1992 Endocrinology and metabolism Vol.7 No.3

We tested apolipoprotein E genotypes in 79 patients with hyperlipidemic diabetes, 44 patients with cerebrovascular accident and acute myocardial infaction, and normal subjects with normolipidemia. We distinguished four different apolipoprotein E genotypes by polymerase chain reaction (PCR) and polyacrylamide gel electrophoresis (PAGE) and determined genotypes and allele frequencies. 1) The frequencies of apo E genotype E3/E4 was higher in the patients with hyperlipidemic diabetes (20.2%) than in the normal subjects with normolipidemia(4.1%) (0.01$lt;P$lt;0.025, X2=10.8). 2) The frequencies of the apo E4 allele was signficantly higher in the hyperlipidemic diabetic patients (12.7%) than in the normal subjects(6.1%) (0.005$lt;P$lt;0.01, X2=12.7). 3) In patients with CVA and acute MI the frequencies of apo E genotype E3E4 and E4 allele tended to be higher in the hypercholesterolemia (40.0%, 20.0%) than in the normocholesterolemia(3.8%, 2.0%) P$lt;0.002, X2=14.7). These data suggest that Apo E genotype E3/E4 and E4 allele are one of predisposing factors to hypercholesterolemia in patients with diabetes, cerebrovascular accident, and acute myocardial infarction.(J Kor Soc Endocrinol 7:273~279, 1992)

Graves병 환자의 HLA-DQ 유전자의 제한효소 분절 길이의 다형성

김진우,팽정령,최영길,김영설,김광원,양인명,김성운 대한내분비학회 1990 Endocrinology and metabolism Vol.5 No.1

The distributions of HLA-DR and DQ antigen were compared between 40 patients with Graves' disease and 24 normal controls. The frequency of HLA-DR 7 among 16 types of HLA-DR was significantly lower in the patient group. And the frequency of HLA-DQw4 among 6 types of HLA-DQ was significantly higher in the patients group.We compared the restriction fragments of HLA-DQ DNA digested with Bam HI and Taq I and hybridized with DQ cDNA between 10 patients and 10 normal controls who were homozygotes of HLA-DQw1 or DQw6. There was no band showing significant difference in the frequency. These data suggest that the pattern of linkage between HLA class II antigens and the disease susceptibility in Korean is different from that in Caucacian, and a gene responsible for disease suceptibility is not located on DQ gene (J. Kor Soc Endocrinol 5:29~36, 1990).

홍삼 복합제의 체지방 감소 작용 기전의 연구

신현대,박철영,팽정령,오승준,김영설,김성운,양인명,김진우,최영길 대한비만학회 2000 The Korean journal of obesity Vol.9 No.4

The effects of red ginseng compound preparation in weight loss have been confirmed, though its specific mechanism is unclear. The changes in both clinicai and lab results show body fat reduction through increase in energy metabolism after the administration. Therefore the effects of red ginseng compound preparation are likely to be caused by activation of sympathetic nervous system by beta-adrenalin system. The experiment was done in order to confirm this hypothesis on cellular level by administrating red ginseng compound preparation and extracting peripheral WBC to analyze adrenalin activation and its receptor RNA. Calorie-restricted diet and red ginseng compound preparation were given to 20 obese patients (BMI $gt; 27 kg/㎡) and 10 controls for duration of 4 weeks. (male to female ratio was 5:15) Changes in weight were from 80.5±15.3 kg (base line) to 79.2±14.7 kg (after taking red ginseng compound preparation) in the patient group, but in the control group did not decrease significantly. In similar sense, Bdecreased from 30.9±4.5 kg/㎡ (base line) to 30.4±4.3 kg/㎡ (after taking red ginseng compound preparation) in the patient group. However, BMI was not decreased in control group. Waist hip ratio was unchanged in both groups. The percent body fat was 35.16% (base line), 33.87% (after dieting), and 31.68% (after taking red ginseng compound preparation). Plasma epinephrine was increased in the patient group from 0.15±0.08ng/mL to 0.21±0.07 ng/mL, but in the contral group, the epinephrine level was decreased from 0.17±0.07 ng/mL to 0.14±0.05 ng/mL. The plasma norepinephrine levels of both groups showed no particular difference. To observe the possibility of 3 receptor expression, RT-PCR was done before and after drug administration. The results showed significant increase in receptor expression in both groups, in patient group increased from 0.87±0.45 to 1.79±0.78, and also increased in the control group from 0.62±0.33 to 0.91±0.53. The increase is quite significant in the patient group. The cAMP prod reflects the activity of beta-adrenaline was 10.1±6.1 S.I in patient group and 7.1±3.9 S.I in the control before drug administration. After administration, these changed to 12.3±6.1 S.I in the patient group and 4.8±1.4 S.I in the control group. Although this seems to show some increase in the patient group, it had no significant meaning statistically. The results shown above showed that the effects of red ginseng compound preparation in weight loss seem to result from increase in beta adrenaline activity, though this has not much meaning statistically. In particular, when we look at the increase of beta 3 adrenaline receptors in patient group, it can be that the effect of red ginseng compound preparation is lipolysis through the beta 3 receptor.