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Kim-Ngan T. Tran,쿠마라벨 아쇽 쿠마,정재훈,홍순호 한국생물공학회 2022 Biotechnology and Bioprocess Engineering Vol.27 No.2
L-serine is a non-essential amino acid which has a wide range of applications and plays an important role as a building block for growing cells. L-serine microbial development is considered a difficult activity due to Lserine's central role in cellular metabolism with 2 main degradation pathways. A novel strategy is needed to overcome the L-serine degradation pathway and low Lserine tolerance of Escherichia coli for efficient L-serine production. A synthetic protein scaffold between SerB and EamA was introduced in this study to physically combine the two enzymes. Through this strategy, the L-serine production is more efficient than in competing pathways. By the introduction of a synthetic protein scaffold without metabolic pathway engineering or addition of glycine, 1.8 g/L of L-serine was produced at pH7 and 37°C. By fermentation, 9.4 g/L of serine was produced at a yield of 0.34 mol/mol glucose. These results suggest that the carbon flux was successfully directed to the L-serine secretion pathway without knocking out a competing pathway or adding expensive glycine.
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Impact of the Synthetic Scaffold Strategy on the Metabolic Pathway Engineering
Kim-Ngan T. Tran,쿠마라벨 아쇽 쿠마,홍순호 한국생물공학회 2023 Biotechnology and Bioprocess Engineering Vol.28 No.3
For the development of the efficient bio-refinery process or biochemical producer, metabolic engineering has become an attractive choice recently. However, engineered metabolic pathways often suffer from flux imbalances due to a lack of corresponding regulatory mechanisms associated with natural metabolism. The interaction among different enzymes within a metabolic pathway plays an important role in regulating the efficiency of metabolic processes. Consequently, the creation of protein scaffolds has helped with the spatial co-localization of proteins in metabolic engineering. Research on protein scaffolds indicated scaffold systems may enhance metabolic productivity further. In this review, the specificity, selectivity, and regulatory mechanisms of protein-protein interactions are discussed in the context of the important effects that they exert on various biological processes.
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