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둥근성게(Mesocentrotus nudus)와 말똥성게(Hemicentrotus pulcherrimus)의 수정 및 정상 배아발생률을 이용한 Phenanthrene의 독성영향
최훈,이주욱,박윤호,이승민,최윤석,허승,황운기 한국환경생물학회 2020 환경생물 : 환경생물학회지 Vol.38 No.3
The aim of this study was to define the toxic effects of phenanthrene (PAHs) on the fertilization and normal embryogenesis rates in the two species of sea urchin (Hemicentrotus pulcherrimus and Mesocentrotus nudus). The sperm and fertilized eggs of both sea urchin species were exposed to serial dilutions of phenanthrene for 10 min and 48 hours, respectively. The fertilization rate and normal embryogenesis rate of H. pulcherrimus and M. nudus were decreased in a concentration-dependent manner. The EC50 for the fertilization rate of H. pulcherrimus and M. nudus was 17.48 mg L-1 and 16.21 mg L-1, and the EC50 for the normal embryogenesis rate was 2.99 mg L-1 and 0.36 mg L-1, respectively. Between the two species, H. pulcherrimus was more sensitive to phenanthrene exposure, and 48 h normal embryogenesis was the more sensitive endpoint. Therefore, the results of this study demonstrated that the exposure of both sea urchin species to phenanthrene caused alterations in egg fertilization and the early developmental stages. 본 연구에서는 다환방향족탄화수소 (PAHs) 중, phenanthrene이 둥근성게 (Mesocentrotus nudus)와 말똥성게 (Hemicentrotus pulcherrimus)의 수정률과 정상유생 발생률에 미치는 독성 효과를 확인하고자 하였다. H. pulcherrimus와 M. nudus의 모체에서 각각 획득한 정자와, 인공수정을 통하여 획득된 수정란을 phenanthrene에 노출시킨 후, 수정률과 정상배아 발생률을 측정하였다. 시험결과, H. pulcherrimus와 M. nudus의 수정률과 정상배아 발생률은, 농도 의존적으로 감소하였으며, H. pulcherrimus와 M. nudus의 수정률에 대한 EC50값은 17.48 mg L-1과 16.21 mg L-1이었고, 정상배아 발생률의 EC50값은 각각 2.99 mg L-1과 0.36 mg L-1인 것으로 나타났다. 연구결과, H. pulcherrimus는 M. nudus보다 phenanthrene 노출에 대하여 더 민감하게 반응하는 것으로 나타났으며, 정상배아 발생률은 수정률에 비하여 더 민감한 종말점인 것으로 나타났다. 따라서, phenanthrene은 두 종의 성게 정자의 수정과 초기발달 단계를 포함한, 연안에 서식하는 다양한 생물종에 영향을 미치는 것으로 보여진다. 그중, 두 종의 성게는 다른 연안 서식 생물 종들에 비하여, Phenanthrene의 bio-monitoring을 위한 민감한 생물종일 수 있다고 사료된다. 또한, 본 연구를 통하여 도출된 결과와 독성값 (NOEC, LOEC 및 EC50)은 Phenanthrene을 포함한 PAHs의 환경 기준농도 설정을 위한 유용한 기초 자료로 활용될 수 있다.
남성&여성 당뇨인, 그들만의 고민- 당뇨병 환자의 임신
최훈,Choe, Hun 사단법인 한국당뇨협회 2005 당뇨 Vol.189 No.-
당뇨병은 임신의 금기증이 절대 아니다. 당뇨병이 있는 가임의 여성은 임신 전부터 철저한 혈당 조절과 산과의와의 상담을 통해 계획된 임신을 하고, 임신기간 동안 적극적인 산전진찰과 체계적인 혈당관리를 한다면 건강한 태아를 분만할 수 있다. 무엇보다 임신전과 임신 전반기를 통한 혈당조절이 가장 중요하다는 것을 명심해야 할 것이다.
대학생이 인지하는 크루즈 여행의 제약요인과 태도가 여행의도에 미치는 영향
최훈,이광옥 한국해양관광학회 2019 해양관광학연구 Vol.12 No.2
Although a number of studies have been done exploring the satisfaction of cruise tourism, little investigation has been done of discouraging factors of cruise tourism at universities. The purpose of this study was to gather information how college students perceive discouraging factors of cruise tourism and find out the relationship between discouraging factors of cruise tourism and students' attitude and intention of cruise tourism. 250 students was chosen for questionnaire survey. The survey was conducted on November, 2019. Various inferential statistics were used as appropriate to test hypotheses. Major findings of the study were as follows: 1. 60 percent of respondents traveled abroad and they prefer to cruise western Europe, Mediterranean, and Northern Europe. 2. Most respondents tended to view that they would invest time and money for cruise travel, and would plan for cruise as security is guaranteed and interest for cruise travel is increased. This result supported Chun(2015) and Yoon's(2016) study. 3. The result showed a statistically positive correlation between perceived the attitude of cruise's travel and intention of future cruise traveling.
자발성 두개내 저압 환자에서 뇌척수액 누출부위에 근접하여 재시행한 경막외 혈액 봉합술에 의한 치료 경험 1예
최훈,정일상,한영진,손지선 대한마취통증의학회 2005 Korean Journal of Anesthesiology Vol.49 No.4
Spontaneous intracranial hypotension (SIH) occurs without any preceding events such as lumbar puncture, surgery, trauma, or medical illness. It is characterized by a postural headache that is aggravated in the erect or sitting position, and relieved in the supine position. A postural headache usually resolves either spontaneously or with conservative treatment. An autologous epidural blood patch (EBP) is an effective strategy for managing a postural headache that persists or is aggravated despite conservative treatments. We report a case of SIH with confirmed cerebrospinal fluid (CSF) leakage at the thoracic level, which was managed successfully with an autologous EBP at the lumbar and thoracic levels. We believe that success rate of an epidural blood patch in these cases is dependent on the approximation of the blood injection to the leak site.
최훈,기순종,정석구,이준예 대한마취과학회 1980 Korean Journal of Anesthesiology Vol.13 No.4
Endotracheal intubation has been a routine practice in general anesthesia and is accepted by anesthetiste and surgeons as an integral part of airway management during anesthesia and operation. Paradoxically however, there have been several cases of obstruction of the airway which occured due to endotracheal intubation. The authors experienced two cases of airway obstruction due tendotracheal and endobronchial techniques, using tubes with malfunctioning cuffs. The lumen of an endotracheal tube was collapsed by intracuff pressure, shortly after inflation of the cuff and resulted in extremely high airway resistance. In the other case, gradual leakage of air from the distal cuff of a Carlens tube led to collapse of the distal cuff. This promoted the movement of blood, secretions, and pus from the diseased right lung to the normal healthy left lung in association with the left lateral recumbent position, which in turn resulted in total airway obstruction.