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Virulence Differentiation of Eight Turnip mosaic virus Isolates Infecting Cruciferous Crops
최홍수,윤무경,최장경,김국형,천정욱,Seong-Han Sohn,Jeong-Soo Kim,Hassan Karakacha Were,Yoichi Takanami 한국식물병리학회 2005 Plant Pathology Journal Vol.21 No.4
Turnip mosaic virus (TuMV) is an infectious viral pathogen on the cruciferous crops, predominantly Chinese cabbage (Brassica campestris subsp. pekinensis) and radish (Raphanus sativus). On the basis of the symptom development in selective differential hosts from indicator host species, Chinese cabbage and Korean radish inbred lines, the representative eight isolates of TuMV were divided into two major groups/or six types. Group I includes Tu 1, Ca-ad7, and Cj-ca2-1 isolates, while group II includes the other isolates (rg-pf1, r 9-10, Rhcq1- 2, Stock and Mustard). According to the molecular phylogenetic analysis, these isolates, however, divided into two groups and two independent isolates. Phylogenetic analysis indicated that four isolates (Tu 1, r 9-0,Stock and Rh-cq1-2) formed a distinct phylogenetic group, and the other two isolates (Ca-ad7 and Cj-ca2-1) also formed another group. Mustard and rg-pf1 isolates did not seem to have any relationship with these two groups. Taken together, these results indicated that virulence differentiation on host plants, molecular phylogenetic analysis of the nucleotide and the deduced amino acid of TuMV coat proteins did not show any relationship. The multi-resistant lines, Wonyae 20026 and BP058 in Chinese cabbage represent valuable genetic materials that can be used for crucifer breeding programs on TuMV resistance, but not in Korean radish.
Characteristics of Potato virus Y Isolated from Paprika in Korea
최홍수,김미경,박진우,김국형,고숙주,최장경,Su-Heon Lee,Hassan Karakacha Were,Yoichi Takanami 한국식물병리학회 2005 Plant Pathology Journal Vol.21 No.4
A virus isolate collected from infected paprika (Capsicum annuum var. grossum) was characterized as Potato virus Y (PVY) based on biological, serological, cytopathological, and molecular properties. In host range studies, the paprika isolate produced the mosaic symptom on some tobacco, tomato and pepper (Capsicum annuum). A new paprika isolate also infected potato cultivars which is different biological characteristic compared to the other popular potyvirus infecting paprika, Pepper mottle virus (PepMoV). Previously reported PVY strains, PVYO and PVYN did not infect pepper and typical PepMoV isolates did not infect potato. Distinctive inclusion patterns of the scroll, pinwheel, long laminated inclusions, and helper components in the cytoplasm of infected cells were also different to those observed by the typical PVY isolate infections. However, the paprika isolate reacted to the monoclonal antibody of PVYN strain with high absorbance readings. RT-PCR amplification, cloning,and sequencing of the 3' untranslated region and a part of coat protein gene also added additional evidence of the paprika isolate as the PVYN-related isolate. Multiple alignments as well as cluster dendrograms of PVYpaprika isolate revealed close phylogenetic relationship to the PVYN subgroup. Altogether, these results suggest that a new PVY isolate infecting paprika contained distinct characteristics compared to the other previously described PVY strains with closer relationship to the PVYN strain.
崔洪守 慶尙大學校 1984 論文集 Vol.23 No.2
Optical absorption, thermally stimulated luminescence(TSL) and TSL emission spectra of uv-grade single-crystal Al₂O₃have been conducted in the temperature range 77-300K. Two kinds of samples was studied. CS sample had been grown in reduced atomosphere environment and Linde sample in oxidizing environment. A typical absorption curve of CS sample exhibits a intense peak near 6.1eV. Linde sample shows a intense peak near 6eV and two weak peaks at 5.4eV and 4.8eV after neutron irradiation. Glow curves of CS sample resulting from uv irradiation at 77k exhibit maxima at 258K (thermal activation energy calculated by Initial Rise method: E=0.72eV, emission spectrum peak:410nm) and 285K(E could not be deduced). A new peak was appeared at 225K(E=0.56eV, 410nm) when bleached at 200K. From Linde crystal, a intense glow peak was observed at 285K(E=0.77eV, 320nm) and weak one at 220K(E=0.53eV, 315nm). The dependence of the intensity of glow peaks on the excitation wavelength was also examined. Experimental results suggest that the 258K and 225K peaks of CS sample are due to the thermal release of trapped electrons which recombine with existing F+ centers producing F centers in an excited state. De-excitation of these centers produces near 410nm radiation. The 285K and 220K peaks of Linde sample are attributed to the thermal release of trapped holes that recombine with electrons of the F centers converting them to excited-state F+ centers which relax to the ground state emitting near 320nm radiation.
최홍수,Choe, Hong-Su 한국작물보호협회 2012 자연과 농업 Vol.283 No.-
우리나라 파프리카에 발생하는 주요 바이러스는 오이모자이크바이러스(Cucumber mosaic virus, CMV), 고추모틀바이러스(Pepper mottle virus, PepMoV), 잠두위조바이러스2(Broad bean wilt virus2, BBWV2), 고추약한모틀바이러스(Pepper mild mottle virus, PMMoV), 토마토반점위조바이러스(Tomato spotted wilt virus, TSWV) 및 사탕무황화바이러스(Beet western yellows virus, BWYV) 등 6종이다. 생산량 감소 및 고품질 규격품 생산의 가장 큰 제한요인이 되고 있는 바이러스병의 피해를 최소화하기 하기 위하여 바이러스별 병징 및 발생원인에 따른 예방법을 알아본다.