Studies on the Degree of Mixing in Air Lift Fermentor

최태부,이준식,유두영 한국화학공학회 1978 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.16 No.5

운동요법과 약물요법을 통한 비만 개선 비교 연구

최태부,김유정 한국피부과학연구원 2010 대한피부미용학회지 Vol.8 No.1

The study of obesity among adult women want to exercise, find fitness center, select the name and 70 to select the 70 people targeted drug therapies conducted four weeks later, body weight, BMI, body fat by measuring the change by comparing the two treatments Learn to manage the effects of obesity for each experiment, the experimenter selected the average age of military age in therapy and was able to predict the rating obtained the following conclusions. 1. Changes in body weight exercise reduction of 0.70Kg. There was no significant difference was (p <0.05) changes in drug therapy group weight loss before and after the experiment 61.38.Kg in 65.32.Kg. 2. BMI changes in both the two treatments were significantly different (p <0.05), drug therapy, especially before the experiment group and 26.11 ± 3.98Kg/m2 the experiment compared to 24.67 ± 3.59Kg/m2 to exercise more to reduce differences in said. 3. Body fat changes in the two treatments, but both have significant differences (p <0.1), drug therapy before the experiment, 36.39 ± 6.42%, to 33.82 ± 6.60% after the experiment of the effects found to be reduced. 4. Exercise therapy to control obesity, ages 20 to 30 units the exact distribution was confirmed with plenty of 20s units tested before and after weight 0.76kg, BMI was 0.28Kf/m2, body fat 0.17% of the difference was significant (p <0.01), 30s 40s, weight, BMI, body fat could see the effect of the reduction. 50s units of weight and BMI before and after testing to see the effect of the reduction, but the test body fat than I was then an increase of 0.35%.(P <0.01)5. Drug therapy, 70 subjects selected on-killing of 30 women were able to determine the distribution of preferences. 20s and before and after the test weight, BMI, body fat it had the effect of reducing both the weight of 3.24Kg, BMI was 1.23Kg/m2, body fat 2.75% of the average number was reduced. (P <0.01) 30s before and after the test weight, BMI, body fat it had the effect of reducing both the weight of 3.86Kg, BMI was 1.55Kg/m2, body fat a 2.93% reduction of the average number of was no significant difference (p <0.01) The study of exercise and drug therapy for obesity, to compare improvements based on more realistic basis for obesity how to find hope. 본 연구는 비만관리방법 중 운동요법과 약물요법을 선택한 70명을 대상으로 4주간 실시한 후, 체중, BMI, 체지방률의 변화를 측정, 비교함으로써 비만관리에 미치는 효과를 알아보기 위한 실험으로 다음과 같은 결론을 얻었다. 1. 체중의 변화는 운동요법은 0.70Kg감소하여 유의한 차이가 있었고(p<0.05 ) 약물요법은 65.32.Kg에서 61.38.Kg으로 실험 전후 감소 효과가 컸다. 2. BMI의 변화는 두 요법 모두 유의한 차이가 있었고(p<0.05) 특히 약물요법이 실험 전 26.11±3.98Kg/m2 에서 실험 후24.67±3.59Kg/m2로 운동요법에 비해 더 많은 감소의 차이를 보였다. 3. 체지방률의 변화는 두 요법 모두 유의한 차이가 있었으나(p<0.1) 약물요법이 실험 전 36.39±6.42%, 실험 후 33.82±6.60%로 감소의 효과가 큰 것으로 나타났다. 4. 운동20대와 30대가 많음을 확인하였고, 20대의 체중은 0.76kg, BMI는 0.28Kf/m2, 체지방률은 0.17%감소, 30대와 40대는 세 항목 모두 감소, 50대의 체중과 BMI는 감소의 효과를 볼 수 있었으나 체지방률은 시험전보다 후에 0.35%의 증가율을 보였다. (p<0.01)5. 약물요법은 30대이상이 많음을 확인할 수 있었고 20대의 체중은 3.24kg, BMI는 1.23Kg/m2, 체지방률은 2.75%, 30대,40대, 50대에서도 시험 전과 후 세 가지 항목 모두에서 유의한 차이가 있었다(p<0.01)본 연구는 운동요법과 약물요법의 비만 개선 효과를 비교해보고 좀 더 현실에 근거한 비만관리 방법을 찾기 위한 기초자료가 되기를 바란다.

Determination of Tyrosinase mRNA in Melanoma by Reverse Transcription-PCR and Optical Mirror Resonance Biosensor

최태부,Inchul Park,Seokil Hong 한국생물공학회 2002 Biotechnology and Bioprocess Engineering Vol.7 No.4

Tyrosinase transcript in the blood is known as the marker of malignant melanoma and it has been often determined by using reverse transcription-polymerase chain reaction (RT-PCR). However, after the PCR process, the quantification of amplified cDNA by the gel electrophoresis is not reliable and time-consuming. For this reason, we tried to quantify the PCR product using a cuvette-type biosensor, where the oligonucleotide probe was immobilized on the cuvette surface and the single strand cDNA, the denatured PCR product, was then hybridized onto the immobilized probe to give a response signal. The response was immediate and takes 15 min to obtain a stable signal. The biosensor was much more sensitive comparing to the gel electrophoresis method. The quantification of PCR product using a cuvette-type biosensor was feasible and rapid.

Change of Insulin-like Growth Factor Gene Expression in Chinese Hamster Ovary Cells Cultured in Serum-free Media

최태부,박홍우,안성관 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.4

총설 : 단일클론항체의 생산과 이용

최태부 한국화학공학회 1987 NICE Vol.5 No.2

리포좀을 이용한 암치료 제제의 개발

최태부 건국대학교 유전공학연구소 1993 제1회 건국대학교-KIST유전공학연구소 공동심포지움 Vol.- No.-

양이온 리포좀을 이용한 유전자 전달 및 발현서 첨가제의 효과

최태부,박인철,홍석일,C. Schmid 한국생물공학회 1998 KSBB Journal Vol.13 No.4

Cellular transfections with cationic liposomes are widely empolyed for gene and oligonucleotide transfer in vitro because of their safety and ease of use. However, they still suffer from the low transfection efficiency comparing with viral vectors. Substantial effort shave been focused on increasing transfection efficiency by supplementing the liposome/DNA complexes(lipoplex) with various components. In this work, we tired three kinds of supplements, Poly-L-lysine(PLL), transferrin and a mixture of anionic lipids(PS/PE/PC), to study their effects on gene transfer yield and gene expression efficiency. PLL, a polycationic polymer, enhanced gene transfer yield by 3 times but the gene expression efficiency was increased only by 1.5 times. this result implies that PLL can enhance the transfection efficiency mainly by increasing the rate of outermembrane transport of lipoplex into the cells. On the other hand, transferrin which can facilitate the gene transfer via ligand-receptor interaction gave not only increased gene transfer yield but also enhanced gen expression efficiency by 2.8 times. Transferrin seems to contribute to the escape of plasmid from endosomes through ligand-receptor recycle mechanism. When the cells were treated with a mixture of anionic lipids for 3 hours before the transfection, gene transfer yield was slightly decreased but the gene expression efficiency was enhanced by 1.9 times. This is presumably due to the accelerated liposome-plasmid dissociation by the anionic lipids, and the increased delivery of plasmid to the nucleus. According to these results, it is clear that the supplementation to ameliorate transfection efficiency with cationic liposomes should be contrived in the direction of increasing delivery of plasmid.

연구발표논문초록 ( 생물화공 (Ⅰ) ) : 동물세포의 배양을 위한 폴리아크릴아마이드 미립담체의 개발

최태부,박선호,천승조,박형상 ( T . B . Choe,S . H . Park,S . H . Chun,H . S . Park ) 한국화학공학회 1988 춘계학술발표회 Vol.- No.-

위벽세포에 대한 헬리코박터 파이로리의 부착 및 탈착에 관한 연구

구재경,최태부 건국대학교 산업기술연구원 1999 건국기술연구논문지 Vol.24 No.-

H. pylori를 닭에 면역접종을 하여 생산된 난황 항체는 면역접종을 하지 않은 군에 비해 10배 이상의 H. pylori에 대한 특이항체가 생산되었다. 난황 항체를 λ-carrageenan을 이용하여 정제한 결과 약 70-75% 정도의 순도로 조정제되었다. H. pylori가 위 상피세포에 결합하는 현상이 H. pylori의 감염에 중요한 첫 단계라는 점에 착안하여 H. pylori의 위벽세포에 대한 결합의 특성을 조사하는 한편, 난황 항체 및 어려가지 부착억제후보물질을 이용하여 H. pylori의 부착 억제 및 탈착을 할 수 있는지에 대하여 실험하였다. 부착의 특성은 positive-cooperative한 결합을 하는 것으로 나타났으며 부착억제 실험의 결과, 0.5mg/ml의 난황 항체를 이용하였을 때 AGS에 대한 H. pylori의 부착을 90% 가량 저해하거나 AGS에 이미 부착된 H.pylori를 80%이상 탈착시킬 수 있었다. 그러나 난황 항체에 의한 H. pylori의 부착억제나 탈착은 균주에 따라 그 효과가 달라지므로 앞으로 이를 보완하는 실험이 필요하다. 부착억제 후보 물질의 탐색결과, GPI 및 dextran sulfate 등 몇 가지 유용한 후보 물질의 선별이 가능하였다. 하지만 앞서 기술한 바와 같이 H. pylori의 결합에 관계하는 receptors 및 molecules가 어떤 특정한 한가지가 아니라 여러 가지의 복합적인 물질이 관여 하는 것이기 때문에 여기에 대해서는 더 많은 연구가 뒷받침되어야 할 것으로 사료된다. H. pylori known as a key pathogen for chronic gastric and duodenal ulcers. Egg yolk antibody, IgY produced from chicken immunized with H. pylori antigen was tested for the inhibition of adhesion of H. pylori to gastric epithelial cell, AGS, and for the detachment of H. pylori adhered to AGS in vitro. We also characterized the pattern of H. pylori adherence to AGS cell line, which was positive-cooperative interaction. In the adhesion inhibition and detachment study, the inhibition of H. pylori adhesion to AGS was as high as 90% using 0.5mg/ml of antibody only and detachment was also achieved almost 80%. Inhibitory effect of adhesion-inhibiting candidates was investigated, and GP1 and dextran sulfate was very effective. However, this effect was severely dependant on the H. pylori strains tested. Further studies are necessary to employ the egg yolk antibodies for the treatment of H. pylori in vivo.

효모에서 분리한 멜라닌 생성 억제 물질의 세포분화 억제

최태부 ( Taeboo Choe ),이승선 ( Seungsun Lee ),정호권 ( Hokwon Jung ),오철 ( Oh Chul ) 대한화장품학회 2005 대한화장품학회지 Vol.31 No.1

본 연구에서는 효모에서 분리한 melanoston이라고 명명된 멜라닌 생성을 억제하는 물질의 작용 기전을 밝히기 위한 것이다. α-MSH를 처리한 B16 melanoma 세포에서 melanoston은 tyrosinase mRNA 발현양을 10% 정도 저해되는데 그쳤으며 western blotting을 이용한 단백질 측정에서도 이와 비슷한 정도의 단백질 생성 억제를 보였다. 그러나 B16 세포 배양액에 melanoston을 첨가할 경우 세포내 tyrosinase 활성이 30%까지 감소되는 것으로 나타나 melanoston이 tyrosinase inhibitor는 아니지만 세포내 tyrosinase 활성화(activation) 과정을 억제하는 것으로 추측할 수 있었다. 또한 광학 현미경을 이용한 morphology 관찰에서 α-MSH를 처리한 세포에서는 많은 dentrite가 형성되면서 세포분화가 일어나는 반면 melanoston를 처리한 경우에는 dendrite가 감소하면서 세포형태가 대조군과 비슷하게 회복되는 것을 알 수 있었다. 또 FITC-anti-tyrosinase-Ab를 이용한 형광염색을 통해서는 α-MSH만 처리한 세포에서는 tyrosinase의 분포가 dendrite를 포함한 세포 전체로 퍼져나가는 것을 관찰 할 수 있었고 α-MSH와 melanoston을 동시에 처리한 세포에서는 대조군과 비슷하게 tyrosinase가 핵 주변에서만 관찰되어 melanoston이 B16 melanoma 세포의 분화과정에서 이를 억제하는 효과를 주고 있음을 알 수 있었다. 이상의 결과들을 종합해 볼 때 melanoston은 α-MSH에 의해 진행되는 B16 세포의 분화를 억제하고 이 과정에서 멜라닌 생성의 주된 효소인 tyrosinase의 활성화를 억제하며 결과적으로는 멜라닌 생성을 저해하는 것으로 사료된다. Melanocytes synthesize melanin within discrete organelle termed melanosomes which are transferred to the surrounding keratinocytes and can be produced in varying sizes, numbers and densities. Skin whitening products have become increasingly popular in the past few years. The most successful natural skin whitening agents are: arbutin, vitamin C, kojic acid, and mulberry, which are all tyrosinase inhibitors. In this work, melanoston, a melanogenesis inhibitor isolated from yeast was studied to understand its mechanism of melanogenesis inhibition. It was found that melanoston was not a tyrosinase inhibitor, while when melanoston was applied to the B16 melanoma cell culture media, the intracellular tyrosinase activity was decreased by more than 30%. When B16 melanoma was stimulated with α-MSH, cell morphololgy was dramatically changed to have lots of dendrites on the cell membrane surface. On the other hand, B16 was treated with α-MSH and melanoston, simultaneously, the change of cell morphologv was not so great. This inhibitory effect of melanoston was found to be related to the inhibition of intracellar activation and transportation of tyrosinase, which was observed by irmmunostaining of B16 melanoma using anti-tyrosinase antibody. From these results, melanoston was regarded as an inhibitor to the differentiation of melanoma cells.