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질내가검물 및 소변에서 중합효소연쇄반응을 이용한 Chlamydia trachomatis 검사
최철석 ( Cheol Seok Choi ),성상철 ( Sang Chul Seong ),이무주 ( Mu Ju Lee ) 대한임상검사과학회 1993 대한임상검사과학회지(KJCLS) Vol.25 No.1
A practical test using polymerase chain reac-tion(PCR) for detection of Chlamyadia tracho-matis in clinical samples was performed. Also thets for specificity and sensitivity of proim-ers for 537 base pair fragment were deter-mined. In this study, 20 specimens (15 viginal swabs, 5 urines of men) with non-gonocooccal urethritis (NGU) were subjected to the PCR test. For specificity of test, C. trachomatis serovar (ATCC) for positive control, C. psittaci, N. gonorrheae for negative control were used, respectively. For sensitlvity test, extracted C. trachomatis DNA (36ng/㎕) was serial 10-fold diluted to 10-8. DNA was extracted with phenol/chloroform after proteinase K treatment and precipitated with ethanol. The PCR products were analysed by electrophoresis on 2.0% agarose gels and the DNA was stained with ethidium bromide. Seven (35.0%) of 20 samples tested were PCR positive for C. trachomatis. Of these positive specimens, 5 were viginal swabs, 2 were urine. In specificity of detection, C. trachomatis DNA was amplified with 537 base pair DNA fragment, but all negative control strain DNAs were not amplified. The sensitivity of PCR detection with primers used was as-sessed at 2.6fg of input DNA. In this study the PCR provided sensitive and specific tool for detection f Chlamydia tracho-matis. Accordingly it is our opinon that PCR technique with proper primers is rapid and re- liable tool for the diagnosis of Chlamydia trachomatis infection.
한국의 신생아에서 선천성 대사이상 Screening에 관한 역학조사
이재상,최철석,최규순,이무주,두화선,강상훈,Lee, Jae-Sang,Choi, Cheol-Seok,Choi, Kyu-Soon,Lee, Mu-Ju,Doo, Hwa-Sun,Kang, Sang-Hoon 한국생명보험의학회 1999 保險醫學會誌 Vol.18 No.-
The disorders of congenital metabolic errors causing mental retardation can be prevented by early diagnosis and treatment. We analysed 144,000 neonatal blood samples for phenyketonuria(PKU), maple syrup urine disease(MSUD), homocystinuria(HCU) and histidinuria(HE) by bacterial inhibition method, and galactosemia(GAL) by Paigen method. In our survey, the positive were 4 samples in PKU(4mg/dl;2, 6mg/dl;1, 8mg/dl;1), 8 samples in MSUD(4mg/dl;2, 6mg/dl;2, 8mg/dl;3, 10mg/dl;1), 4 samples in HCU(4mg/dl;2, 6mg/dl;2), 4 samples in Galactosemia(4mg/dl;2, 6mg/dl;1, 8mg/dl;1), respectively. while, no one was positive in HE. The frequncy rate were 1/36,000 for PKU, HCU and GAL, 1/18,000 for MSUD, respectively. But those for HE couldn't be detected in our survey. In this study, the hightest frequency rate was 1/18,000 of MSUD, following were 1/36,000 for PKU, HCU and GAL, respectively. As results our data, it is our opinion that neonatal screening should be performed in terms of national policies for ealy diagnosis and theraphy.