Mouse 피부조직의 계면활성제에 의한 형태학적변화, 면역반응에서의 Protein 및 Lectin변화양상

최정숙 ( Jeong Sook Choi ) 대한화장품학회 1997 대한화장품학회지 Vol.23 No.2

계면활성제에 의해 유도된 흰쥐의 allergy성 접촉피부염에 미치는 영향을 검증하기 위하여 표피의 구조를 광학현미경으로 관찰하였고, 또한 각 항원항체반응에서 단백질의 변화양상을 알아보고자 SDS-PAGE법에 의한 단백질 band의 분석 그리고 피부내에 존재하는 복합당질의 당잔기를 알기 위해 Lectin 반응을 실시하였다. 그 결과는 다음과 같다. 1. 계면활성제의 1일 처리군에서 혈관의 확장이 나타났고 3일 처리군에서 핵이 phknotic되어 세포질의 eosinophlic을 일으켰으며 6일 처리군에서 과각질화, 염증세포의 증가, 표피내로 림프구의 침윤 증가가 관찰되었고 9일 처리군에서 과각질층이 serum crust가 되어 탈락되고 다시 부분적으로 두터워진 Acathosis를 합성한다. 반면에 진피층은 세포내 액포화와 세포사이 공간이 확장되었다. 12일 처리군에서는 표피직하부의 진피층에 혈관이 확장되어 나타나고 표피층은 7층으로 두터워져 생성되며 진피전층에 염증세포의 양이 현저히 감소되었음을 관찰하였다. 2. 계면활성제의 처리군에서 degranulated type histamine, serotonin, hiparin 등의 물질을 함유하고 있는 분비과립이 증가함에 따라 전기영동상에서 나타난 단백질 band의 급격한 변화를 관찰하였다. 3. 피부내 복합당질의 변화는 LCA, PNA, SBA, WGA에서 모두 양성반응을 보였으나, Con-A에서는 음성 반응을 보였다. PNA와 SBA에서는 계면활성제 처리군에 비하여 강한 양성반응을 보였다. Allergic contact dermatitis is a common skin disease resulting from specific immumologic sensitization to topically applied various allergen. Purpose of this study was to investigate skin morphologic chganges by light microscopic, changes of protein band by SDS-PAGE in the skin treated on the surfactant. Furthermore, lectin histochemistry is used to know the chagnges of the terminal sugar of the glycoconjugate in the skin treated on the surfactant. The results were as follows : The 1 day of treated group was indicated the enlargement of capillary, the 3 days of treated group was showed that the cytoplasm was eosinophlic by the pyknotic of nucleus. The 6 days of treated group was observed hyperkeratinoid, increased of inflammation cells to epithelium. The 9 days of treated group was appeares that serum crust was sheded and formed a acanthosis. But dermis layer was cytoplasmic vaculation and enlargement of intercellular space. The 12 days treated group made up new epidermis layer of seven layer and of observed an decreased in quantity of inflammation cells. The mast cell of degranulated type was increased in treated surfactnat. It was secreating granules hold histamin, serotonin and heparin. Accordingly, protein band of electrophortic phase was observed a sudden changes since 3 days treated group. At observeation of the cell-surface glycoconjugates, LCA, PNA, SBA and WGA lectin positive cells but inducated lectin negative cells in Con-A. Furthermore, PNA and SBA showed stroger positive reaction as treated surfactant group.

곰 토템과 언어문화의 유연성 연구

최정숙(Choi jeong sook),정호완(Jeong howan) 한국문화융합학회 2021 문화와 융합 Vol.43 No.1

이 글은, 언어의 문화투영이라는 관점에서 구비문학적 사례와 지명 분포를 통한 제정일치 시기인 단군왕검의 곰 토템과의 유연성을 탐색하고자 하였다. 곰 토템의 방사에 따른 변이형은 신화와 전설, 지명이나 〔고맙다〕로 대변되는 고마(熊)의 단어족과 나라 이름 백제(百濟), 나아가 조상숭배가 효행문화의 원류가 됨을 확인하게 될 것이다. 따라서 고조선 시기 고마(곰) 토템과 제사장 단군의 샤머니즘이 융합된 고조선의 제의문화적인 정체성을 확인하게 될 것이다. 관련하여 그 동안 식민사관이나 동북공정으로 기록과 유물유적에 대한 고려 없이 저질러진 왜곡된 역사관과 궤변을 언어문화의 관점에서 변증하고자 하였다. 곰 토템과 언어문화의 형태 및 의미의 유연성이 검증됨으로써 단군조선의 언어 문화적인 실체성과 조상숭배 문화의 개연성이 제고될 수 있을 것이다. 본론에서 고마(곰) 토템 문화 관련의 신화와 전설 및 보수성이 가장 강한 지명과 국명인 백제(百濟)와의 유연성, 나아가 조상숭배로서의 개연성을 살펴보았다. The purpose of this study is to examine the correlation between Bear Totem and Language Culture, which is the time of culturally-symbolic establishment of language. The radial form of the bear totem will be identified in the word family of Goma(bear), which is expressed by place name or gomapta(thanks), and the name of the country Baekje(百濟). Therefore, the cultural identity of Gojoseon, which is a fusion of Goma(Bear) totem and the shamanism of Dangun(壇君), will be confirmed during the Gojoseon period of Dangun. In this regard, I tried to prove distorted history and riverside that have been committed without consideration of records and relics through colonial or northeast process projected by others. The linguistic and cultural demonstration of Dangun Joseon could be enhanced by revealing the cultural archetype and morphemes of the bear totem. In the main subject, the origin and conservatism of Goma(bear) totem culture are examined and the correlation between the place name and the country Baekje(百濟).

대화 암기와 DVD 더빙을 이용한 영어 수업 사례 연구: 만족도와 자신감 향상을 중심으로

최정숙 ( Jeong Sook Choi ) 글로벌영어교육학회(구 호남영어교육학회) 2013 Studies in English education Vol.18 No.1

The purpose of this case study is to examine the effect of repetition, dialogue memorization, and DVD dubbing on enhancing low-proficiency college English learners` confidence and interest in English learning. Eighty three sophomore students participated in this study. During one semester mandatory English courses, the researcher asked the students to memorize several conversations and demonstrate English dubbing (making the actual sound mute) of DVD interviews of native speakers as class activities. Both quantitative (surveys) and qualitative (interview) research methods were conducted. The results showed that the conversation memorization and DVD dubbing had given positive effects on their learning of English, and the students could gain confidence and interest on English speaking and listening, particularly. In spite of the first reluctance of the students about the demonstration of conversations and dubbing, the students seemed to get used to and even enjoyed the activities as time goes by. Overall conclusion and some suggestions for more effective English teaching and further studies were added at the end of the paper.

연구논문 : 1-(2-cyclohexylmethoxy-6-hydroxyphenyl)-3-(4-hydroxymethylphenyl)-propenone의 미백효능 및 작용기전

최현숙 ( Hyun Sook Choi ),우미희 ( Mi Hee Woo ),최정숙 ( Jeong Sook Choi ) 한국의류산업학회 2006 한국의류산업학회지 Vol.8 No.3

예비사회복지사의 사회복지현장실습에서의 성찰경험에 관한 질적연구

김정의(Jeong Ui Kim),최정숙(Jung Sook Choi) 충남대학교 사회과학연구소 2024 사회과학연구 Vol.35 No.1

This study aimed to understand the content and significance of reflection experiences during the social work practicum of preliminary social workers, and to provide discussions and foundational material for enhancing the reflective competence of preliminary social workers and social work field practicum education. The participants comprised seven undergraduate students majoring in social work who had completed their social work field practicum at least once between March 1, 2021, and February 28, 2023. Data were collected through in-depth interviews and analyzed using Giorgis phenomenological methods. The analysis resulted in 8 components, 31 subcomponents, and 115 meaning units. The derived components were as follows: ‘Integration of pre-practice experiences distinct from volunteer activities.’, ‘Confirming, applying, expanding, and considering improvements to the knowledge learned in school.’, ‘Opportunity to expand understanding of clients and strengthen relational skills.’, ‘Realization of the importance of community collaboration.’, ‘Difficulties and regrets encountered during field practicum.’, ‘Time for self-reflection and personal growth.’ and ‘Contemplation of career choices while learning and applying professional tasks as future experts.’. Based on the study results, it is suggested that a discussion on reflective practical learning for preliminary social workers at the field practicum and school curriculum levels is necessary. Furthermore, there is a need to create a practicum environment and implement a well-prepared practicum supervision plan within organizations.

공기청정기를 이용한 규모별 의료기관 실내공기질 개선 연구

전현우(Hyunwoo Jeon),최정숙(Joung-Sook Choi),손종렬(Jong-Ryeul Sohn),정나나(Nana Jeong),김주혁(Juhyeok Kim) 대한환경공학회 2020 대한환경공학회 학술발표논문집 Vol.2020 No.11

흑미 호분층 추출물의 급여가 난소절제 흰쥐의 체내 지방, 혈중 지질 및 관련 호르몬 수준에 미치는 영향

최봉겸(Bong-Kyoum Choi),남송이(Song-Yee Nam),이영민(Young-Min Lee),김정봉(Jung-Bong Kim),최정숙(Jeong-sook Choe),이해정(Hae-Jeung Lee),이성준(Sung-Joon Lee),이성현(Sung-Hyen Lee) 한국콘텐츠학회 2015 한국콘텐츠학회논문지 Vol.15 No.9

본 연구는 흑미(Oryza sativa L.) 호분층 추출물이 난소절제 흰쥐의 체내 지질 및 관련 호르몬 수준에 미치는 영향을 알아보고자 수행되었다. 흰쥐를 Sham군(S), 난소절제 control군(OVX), 난소절제 이소플라본 10 mg/kg 투여군(IF), 난소절제 흑미 30 및 90mg/kg 투여군(BRE-30, BRE?90)으로 나누어 12주 동안 고지방식이(45% calories from fat)를 급여하였을 때, 체중 증가량, 체지방무게 및 비만지수는 OVX군에서 증가하였으나, 이소플라본 및 BRE 투여에 의해 유의하게 감소되었다. 혈중 중성지방은 BRE-90군이 OVX군에 비해 유의적으로 낮았고, 혈청 adiponectin 수준은 BRE군에서 OVX군에 비하여 유의하게 증가하였으며, leptin 수준은 대조군인 OVX군에 비하여 유의적으로 감소하였다. 간 중 총지질과 중성지방 및 콜레스테롤은 유의적이지는 않았으나 BRE 투여군에서 OVX군보다 감소하는 경향을 보였다. 따라서, 흑미 호분층은 난소절제 흰쥐에서 지질 대사를 조절함으로써 비만 및 여성의 폐경기 관련 질병을 예방하는 식품이 될 수 있을 것으로 기대된다. This study was conducted to evaluate suppressive effects of black rice aleurone layer extract (BRE) on body fat, serum lipid, and hormone levels in ovariectomized rats. The rats were divided into five groups (n=8/group): sham operated group (S); ovariectomized control group (OVX); ovariectomized group treated with isoflavone 10 mg/kg B.W. (IF); ovariectomized group treated with BRE 30 or 90mg/kg B.W. (BRE-30 or -90). High fat diet (45% calories from fat) was fed to all rats for 12 weeks. Body weight gain, body fat weight, and adiposity index increased in the OVX group, but they significantly decreased in IF or BRE supplementation. Serum triacylglycerol and leptin levels decreased in BRE groups while serum adiponectin level significantly increased compared to that of the OVX group. Hepatic total lipid, triglyceride, and cholesterol levels increased in the OVX group, but they decreased in BRE supplementation (p>0.05). These data suggest that black rice aleurone layer may be a useful food source to decrease obesity and its related diseases by modulating lipid metabolism in estrogen-deficiency model.

농촌노인의 치아상태에 따른 건강상태 및 식이섭취 평가

최희선 ( Hee Seon Choi ),문현경 ( Hyun Kyung Moon ),김혜영 ( Hye Young Kim ),최정숙 ( Jeong Sook Choi ) 대한영양사협회 2010 대한영양사협회 학술지 Vol.16 No.1

In Korea, there has been a rapid increase in the number and proportion of elderly people, especially in rural areas, due to improvements in the standard of living and medical technology. One of the main health problems for the elderly people is dental health, which can cause nutritional and health problems. Thus, in this study, the dental health status and health status in relation to nutritional intake were analyzed. A total of 155 rural-dwelling elderly people (68 males, 87 females) over the ages of 65 participated in this study. The subjects were classified into three groups; the no denture no teeth group, denture user group, and natural teeth group. The dietary intake, biochemical health status, and anthropometry were evaluated. Can-pro 3.0 was used to assess dietary intakes and the SPSS 12.0 program was used for statistical analysis. The results showed that the natural teeth group had better nutritional and dietary intake status than the no denture no teeth group. The dietary assessment showed that there were differences in food intakes among the groups, which depended on their dental health status. In conclusion, dietary management is required for the elderly since each group has a different ability to chew food depending on their dental health status. In addition, the elderly will need different therapeutic diets because of the high prevalence of chronic degenerative diseases.

배암차즈기(Salvia plebeia R.) 에탄올 추출물의 항산화 및 항알레르기 효과

이성현(Lee, Sung Hyen),최봉겸(Choi, Bong Kyoum),이선혜(Lee, Seon Hye),이영민,김정봉(Kim, Jung Bong),장환희(Jang, Hwan Hee),황유진(Hwang, Yu Jin),최정숙(Choe, Jeong sook) 한국지역사회생활과학회 2015 한국지역사회생활과학회 학술대회 자료집 Vol.2015 No.11

지역 농산물의 항산화 및 NO 생성 효과

김주희(Ju-Hui Kim),임정연(Jeong Yeon Im),최활(Hwal Choi),정운율(Un Yul Jeong),김지수(Ji Su Kim),정지은(Jieun Jung),최정숙(Jeong-Sook Choe),이성현(Sung Hyen Lee) 한국지역사회생활과학회 2022 한국지역사회생활과학회지 Vol.33 No.2