돈(豚)에서 분리(分離)한 Shigella균유래(菌由來) R plasmid의 유전적(遺傳的) 특성(特性)에 관한 연구(硏究)

최원필,권해병,정석찬,Choi, Won-pil,Kwun, Hae-byeng,Jung, Suk-chan 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.1

This paper dealt with the distribution of Shigella spp. on 5 piggeries in Taegu and Kyungpook during the period from August to October 1987. Isolated Shigella were examined for serogrouping, antimicrobial drug resistance and detection of R plasmid. Genetic properties of R plasmid in Shigella have examined to fertility inhibition (Fi) and gel electrophoresis was performed for the isolation of plasmid DNA. The results obtained were summarised as followings; 1. Of total 2,978 samples from 5 piggeries, 82 strains (2.8%) of Shigella spp. were isolated from 82 samples. The isolated strains were identified as S dysenteriae (60 strains), S flexneri (20 strains) and S sonnei (2 strains). 2. Of the 82 strains examined 67 (95.1%) were resistant to one or more antibiotics, such as ampicillin (Am), chloramphenicol (Cm), kanamycin (Km), nalidixic acid (Na), rifampicin (Rf), streptomycin (Sm), sulfademethoxine (Su), and tetracycline (Tc) and higher resistant to Su (90.2%), Sm (63.4%) and Tc (63.4%). 3. Of the 78 resistant Shigella strains 26 (33.3%) harbored conjugative R plasmids and the transfer frequency of Sm (50.0%), Cm(33.3%) resistance was much higher than that of the other drug resistance. 4. The most common resistant patterns were SmSuTc, Su and AmSmSuTc. 5. Out of the 26 Shigella R plasm ids examined for Fi, 14(53.8%) were $Fi^+$ and the remainder were $Fi^-$. 6. The plasmid DNA profiles in Shigella spp. (9 strains) isolated from pigs were confirmed as being 2 to 9 fragments by the gel electrophoresis. Their molecular size ranged 2.17 to 87.62 kilobase (Kb). All strains of Shigella spp. consisted in 15.4 Kb plasmids.

우(牛), 돈(豚)에서 분리(分離)한 Salmonella유래(由來) R plasmid의 유전학적(遺傳學的) 및 분자생물학적(分子生物學的) 성상(性狀)에 관한 연구(硏究) II. R plasmid의 비적합성(非適合性) 및 plasmid profile

최원필,이희석,여상건,이헌준,정석찬,Choi, Won-pil,Lee, Hi-suk,Yeo, San-geon,Lee, Hun-jun,Jung, Suk-chan 대한수의학회 1989 大韓獸醫學會誌 Vol.29 No.2

This paper deals with the genetic properties of R plasmids in Salmonella originated from pigs and cattle. The plasmid DNA was examined for incompatibility, stability and fertility inhibition(Fi), and gel electrophoresis was performed for isolation of plasmid DNA. The results obtained were summerized as follows: 1. Among the 66 conjugative R plasmids from 44 pigs and 22 cattle, 61 R plasmids (92.4%) were $Fi^-$, whereas the remainder were $Fi^+$. 2. The Inc groups of 66 R plasmids were determined with 7 standard plasmids. Twenty-six R plasmids were classified into Inc group $I{\alpha}$, H1, H2 or F1, 40 R plasmids being not classified with standard plasmids used, and the Inc group $I{\alpha}$ (57.7%) was most frequent. 3. Inc groups $I{\alpha}$, H1, and F1 were identified in strains from swine, Inc groups H2 and F1 from cattle. 4. The plasmid DNA profiles in 16 Salmonella isolated from pigs and cattle were confirmed as being 1 to 10 fragments by the gel eletrophoresis. Their molecular weight ranged 1.0 to 90 megadalton. 5. The molecular weight of conjugative plasmids ranged 1.0 to 80 megadalton in 4 Salmonella (P-4, P-5, P-7 and P-8) isolated from pigs.

축우의 유산에 관여하는 Aspergilli에 관한 연구

최원필,권해병,여상건,Choi Won Pil,Kwun Hae Byeng,Yeo Sang Geon 대한수의사회 1980 대한수의사회지 Vol.16 No.11·12

The purposes of this study were to determine the incidence of the abortion and vaginitis due to Aspergilli and to research the Aspergilli in the vaginal exudates from apparently healthy dairy cows in the Gyeongbug area. Fetuses from 13 aborted cows, vagin

유우질내(乳牛膣內)의 Aerobic Actinomycetes 의 분포(分布)

최원필,권해병,Choi, Won-Pil,Kwun, Hae-Byeng 대한수의학회 1982 大韓獸醫學會誌 Vol.22 No.1

A bacteriological survey was made for the aerabic Actinonmycetes from the vaginal exudates of apparently healthy dairy cows in Gyeongbug area. One hundred twenty isolated were made from 1,328 cows. Among them 30 isolates (25%) were identified as Nocardia asteriodes, 4 (3.3%) as Nocaradia brasiliensis, and 2 (1.7%) as Nocardia madurae. The remaining 84 isolates (70%) were identified as Streptomyces species.

Survey for Antibodies to Bovine Leukemia Virus in Dairy and Korean Native Cattle

최원필,Choi, Won-Pil The Korean Society of Veterinary Science 1982 大韓獸醫學會誌 Vol.22 No.1

한건(韓件) 및 유우(乳牛)의 우백혈병(牛白血病) 바이러스의 감염상태(感染狀態)와 목장(牧場)의 오염상황(汚染狀況) 등 역학적(疫學的)인 연구(硏究)를 위하여, 경북지방(慶北地方)의 14개목장(個牧場) 유우(乳牛) 106두(頭)와 대구(大邱) 도축장(屠畜場)에서 한우(韓牛) 699두(頭)의 혈청항체(血淸抗體)를 조사(調査)하였다. 우백혈병(牛白血病) 바이러스의 바이러스의 본(本) 바이러스의 단백항원(蛋白抗原)(P)과 당단백항원(糖蛋白抗原)(gp)를 가지고 한천(寒天) Gel 내침강반응(內沈降反應)(ID)을 실시하였고 그 결과(結果)는 다음과 같다. 1. 유우(乳牛) 106두(頭)에 있어서 gp-ID 양성(陽性)인 것은 30두(頭)(28.3%)이었고, 14개목장(個牧場)중 12개(個) 목장(牧場)이 본(本) 바이러스에 오염(汚染)되어 있었으며, 목장별(牧場別) 오염률(汚染率)은 12.5에서 60%로 높은 감염률(感染率)을 나타내었다. 2. 한건(韓件) 699두(頭)에서 gp-ID에 양성(陽性)인 것은 17두(頭)(2.4%)로 낮았다. 3. gp-ID 양성혈청(陽性血淸) 47례(例)중 P 항원(抗原)을 가지고 있는 것은 유우(乳牛) 5두(頭)에서만 인정(認定)되었다.

물범 및 코끼리의 백선균증(白癬菌症)에 관한 연구(硏究)

최원필,Choi, Won Pil 대한수의학회 1981 大韓獸醫學會誌 Vol.21 No.2

This experiment was undertaken to determine the causative agent of dermatophytosis occured in the 3 common seals and a elephant which were derived from the Tae-gu and Busan zoological gardens. Direct microscopic examination, culture and pathogenicity test were performed for the samples obtained from the skin lesions of the affected common seals and elephant. The causative agent was identified as Trichophyton mentagrophytes exclusively in these cases, and the present report describes the first cases of the common beat and elephant ringworm.

Broiler양계사내(養鷄舍內)의 진균총(眞菌叢)

최원필,여상건,이헌준,Choi, Won-pil,Yeo, Sang-geon,Lee, Hun-jun 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.1

The present survey was undertaken to determine the mycoflora of broiler houses. Attempts were made to isolate and identify fungi in the dust, feed, litter and water from 21 broiler houses. A total of 166 isolates of fungi was identified as yeast spp. (44%), Aspergillus spp. (30.7%), Verticillium spp. (7.2%), Penicillium spp. (3.6%), Paecilomyces spp. (3.6%), Scopulariopsis spp. (3.0%), Cephalosporium spp. (3.0%), Chrysosporium spp. (2.4%), Cladosporium spp. (1.8%) and Absidia spp. (0.6%). Isolated of Aspergillus(A) spp. and Penicillium(P) spp. were identified as A. fumigatus, A. flavus, A. terreus, A. nidulans, A. niger, P. citrinum and P. palitan. Fungal contamination of the broiler houses predominated in the dust. The important point is that must of the isolates were pathogenic fungi.

Leptospira 속균(屬菌)에 대한 한우(韓牛) 및 유우(乳牛)의 혈청항체조사(血淸抗體調査)

최원필,이희석,Choi, Won-pil,Lee, Hi-suk 대한수의학회 1985 大韓獸醫學會誌 Vol.25 No.1

A serological survey for antibodies to Leptospira (L.) interrogans in dairy and Korean native cattle in Gyeongbuk area was performed using 6 different living antigens, which were L. icterohaemorrhagiae(RGA), L. canicola(Hond Utrecht IV), L. autumnalis(Akiyami A), L. australis(Ballico), L. pomona(Pomona) and L. hebdomadis(Hebdomadis), by microscopic agglutination test. In the microscopic agglutination test, greater than partial agglutination at a serum dilution of 1:300 or over was recorded as positive. In the dairy cattle(Holstein), four(4.7%) of 86 sera from 13 dairy farms were positive for L. canicola and L. pomona antibodies. In the Korean native cattle, four(3.2%) of 124 sera from the slaughter house in Taegu city were positive for L. canicola, L. icterohaemorrhagiae and L. pomona antibodies. Among the positive sera, L, canicola was dominated in this experiment.

준임상형(準臨床型) 유방염(乳房炎) 및 집합유(集合乳)의 Staphylococcus 속균(屬菌)에 대하여

최원필,박노찬,이강록,Choi, Won-pil,Park, No-chan,Lee, Gang-log 대한수의학회 1986 大韓獸醫學會誌 Vol.26 No.2

This paper deals with the incidence of bovine mastitis for 743 quarters and distribution of Staphylococci for the quarter and 70 bulk milk samples in the northern area of Gyeongbuk during the period from January to December 1984. Isolated Staphylococci were examined for species, subgroups, antibiotic resistance and penicillinase production. The results obtained were summarized as follows : A total of 25(73.5%) of 34 herds, 102(54.3%) of 188 cows and 208(30.3%) of 743 quarters were found to be infected with subclinical mastitis. A total of 83(83.1%) of 102 cows, 94(45.2%) of 208 mastitic quarters and 55(78.6%) of 70 bulk milk samples were isolated Staphylococci. Three hundred and eighteen strains of Staphylococci were classified into 11 species. Of these speoies, S. aureus from mastitis and S. sciuri from bulk milk were found most frequently, followed by S. epidermidis, S. simulans, S. cohnii, S. haemolyticus, S. xylosus, S. hyicus subsp. chromogenes, S. saprophyticus, S. warneri, S hyicus subsp. hyicus. Subgroups of catalase-positive and negative cocci were belonged most frequently to subgroup I, and subgroups III and III b, respectively. The method of Pelzer of al(97.8%) was more classified than that of Baird-Parker (68.5%). One hundred and sixty one strains(50.6%) of 318 Staphylococci isolates were resistance to one or more antibiotics such as ampicillin, chloramphenicol, gentamicin, kanamycin, streptomycin, and tetracycline. Isolates from subclinical mastitis were more resistant to antibiotics than its from bulk milk. Of the 318 Staphylococci Isolates, 128(40.3%) gave positive reaction for the penicillinase test, all of ampicillin resistance strains produced this emzyme.

A Epizootiological Study of Salmonella Infection on Piggery : II. A Study on Drug Resistance and R Plasmids in Salmonella

최원필,이희석,여상건,이헌준,정석찬,Choi, Won-pil,Lee, Hi-suk,Yeo, Sang-geon,Lee, Hun-jun,Jung, Suk-chan The Korean Society of Veterinary Science 1986 大韓獸醫學會誌 Vol.26 No.2

1984년 5월부터 1985년 5월까지 대구(大邱), 경북(慶北), 경남(慶南) 및 충남지역(忠南地域) 7개(個) 양돈장(養豚場)의 자돈(仔豚) 및 성돈(成豚)의 분변(糞便) 및 양돈장(養豚場)의 흙, 하수(下水), 사료(飼料), 추비(推肥), 쥐 등 7,440예(例)와 대구시(大邱市) 도축장(屠畜場)의 도축돈(屠畜豚) 장간막임파절(腸間膜淋巴節) 및 직장내용물(直腸內容物) 555예(例)로부터 분리(分離)한 319주(株)의 Salmonelia속균(屬菌)을 대상으로 항균제(抗菌劑)에 대한 내성(耐性) 및 전달성(傳達性) R plasmid의 분포상황(分布狀況)을 조사(詞査)하였던 바 그 결과(結果)는 다음과 같다. 1. 공시균(供試菌) 319주(株) 중(中) 250주(株)(78.4%)가 ampicillin(An), chloramphenicol(Cm), gentamicin(Gm), kanamycin(Km), nalidixic acid(Na), rifampicin(Rf), streptomycin(Sm), sulfadimethoxine(Su), 또는 tetracycline(Tc)에 내성(耐性)을 나타내었으며, 약제별(藥劑別)로는 Su(74.9%), Sm(53.0%) 및 Tc(28.5%)에 높은 내성(耐性)이 인정(認定)되었다. 2. 내성균(耐性菌) 250주(株)의 전달성(傳達性) R plasmid 보유율(保有率)은 51.2%(128주(株))였으며, 약제별(藥劑別)로는 Am경우 100%, Tc 92. 3% 및 Cm 75.0% 순으로 보유율(保有率)이 높았다. 3. 내성균(耐性菌) 250주(株)의 내성양상(耐性樣相)은 SmSu(91주(株)), Su(59주(株)) 및 TcSmSu(50주(株))내성형(耐性型)이 대부분이었고 R plasmid 전달후(傳達後)의 내성양상(耐性樣相)은 TcSmSu(40주(株)) 및 TcSu(28주(株)) 내성형(耐性型)이 많았다. 4. 양돈장별(養豚場別) 내성균(耐性菌) 출현빈도(出現頻度)는 48.0~93.6%로 다양(多樣)하였고, 전달성(傳達性) R plasmid 보유율(保有率)은 0~77.8%로 내성균(耐性菌) 출현빈도(出現頻度)와 일치(一致)되지 않았다. 5. 공시균(供試菌) 319주(株) 중(中) 각각(各各) 2주(株)는 Rf 및 Gm에 대해 내성(耐性)을 나타내었고, 내성균(耐性菌) 250주(株) 중(中) 73.2%(183주(株))가 다제내성(多劑耐性)이었으므로 Salmonella의 다제내성화(多劑耐性化) 경향(傾向)이 있었다. 6. 내성균(耐性菌) 250주(株) 중(中) R plasmid 전달후(傳達後) 5주(株)는 TcAmCmSmSu내성형(耐性型), 1주(株)는 TcAmKmSmSu내성형(耐性型)임이 확인되었다.