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      • DNA Translocation using Nanoporous Membrane

        최요셉(Yosep Choi),차미선(Misun Cha),이정훈(Junghoon Lee) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.11

        This paper reports an on-chip nano porous membrane that can separate bio-molecules based on their sizes and its working principles. The nano porous membrane is considered as one of key elements used for the separation of macromolecules. In conventional approaches for bio-molecular separation with nano porous membrane, however, there are many limitations such as difficulty in integrating with a lab on a chip (LOC) device and the lack of precision in separations. In this work anodized alumina oxide (AAO) nano porous membrane was directly fabricated on a microfluidic platform to be integrated with LOC. In addition, atomic layer deposition (ALD) was used to reduce the pore size down to 13 ㎚. Bio-molecular trans-location through the AAO by electrokinetics and the effective pore size due to surface charge were investigated. Our efficient and effective on-chip device successfully demonstrated the separation of aptamer-protein complex from DNA.

      • Improvement of Nanosphere Lithography Using Nano Template

        정승원(Seungwon Jung),최요셉(Yosep Choi),이정훈(Junghoon Lee) 대한기계학회 2008 대한기계학회 춘추학술대회 Vol.2008 No.5

        Nanosphere lithography has been studied as a general fabrication method to produce periodic particle array with nanometer scale features. This paper presents the first sub-100 ㎚ nanosphere lithography (NSL) with an optimized spin coating condition and a nano template structure. The spin coating condition was tuned to obtain the best possible crystal formation. The qualities of the crystal domain, i.e., regularity and coverage, were further improved when a nano-trench structure was used as a template. We demonstrate a sub-100 ㎚ nanohole array over 1㎟ area with subsequent fabrication steps.

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