Survey on Insect Fauna and Role of Insect Gardens for Ecotourism

최영철,김종길,최지영,김원태,박해철,황석조,정길상,Choi, Young-Cheol,Kim, Jong-Gill,Choi, Ji-Young,Kim, Won-Tae,Park, Hae-Chul,Hwang, Seok-Jo,Jeong, Gil-Sang Korean Society of Applied Entomology 2009 한국응용곤충학회지 Vol.48 No.4

This study was conducted to investigate insect fauna in the four insect garden sites of Suwon, Yeongyang, Buyeo and Yecheon from 2005 to 2007. Seasonal population size of insects was largest from June to August in all the four sites. In the four sites, Coleopteran insects were dominant followed by Hemiptera and Orthoptera. Unique education/learning programs are successfully run at the insect gardens based on the three geographic types (i.e. urban, mountainous and rural). These activities will help preserve insect biodiversity in the area and visitors better understand life forms such as insects found in the areas. 본 연구는 2005년부터 2007년까지 수원, 영양, 부여와 예천의 곤충 생태원 부지에서 곤충강의 다양성을 조사하였다. 월별 곤충 개체군 크기는 6월부터 8월에 네 조사구역 모두에서 가장 컸다. 전체적으로 딱정벌레목의 곤충이 가장 많이 채집되었으며, 노린재목과 메뚜기목의 곤충이 뒤를 이었다. 각 곤충생태원은 지리적 특색에 맞는 고유한 교육프로그램을 운영하고 있었다. 이러한 연구와 생태원의 활동은 그 지역의 곤충과 같은 생물의 다양성을 보존하고 관람객에게 인식시키는데 중요한 역할을 할 것이다.

가속도계를 이용한 재료의 영계수 측정방법

손창호(Sohn, Chang-Ho),박진호(Park, Jin-Ho),윤두병(Yoon, Doo-Byung),정의필(Chong, Ui-Pil),최영철(Choi, Young-Chul) 한국소음진동공학회 2006 한국소음진동공학회 논문집 Vol.16 No.11

For the description of the elastic properties of linear objects a convenient parameter is the ratio of the stress to the strain, a parameter called the Young's modulus of the material. Young's modulus can be used to predict the elongation or compression of an object as long as the stress is less than the yield strength of the material. Conventional method for estimating Young's modulus measured the ratio of stress to corresponding strain below the proportional limit of a material using a tensile testing machine. But the method needs precision specimens and expensive equipment. In this paper, we proposed method for estimating Young's modulus using accelerometer. The basic idea comes from that the wave velocity is different as the Young's modulus. To obtain Young's modulus, a group velocity is obtained. It is difficult to measure group velocity. This is because plate medium has a dispersive characteristics which has different wave speed as frequency. In this paper, we used Wigner-Ville distribution to measure group velocity. To verify the proposed method, steel and acryl plate experiments have been performed. Experimental results show that the proposed method is powerful for estimating Young's modulus.

감염 B 형 바이러스의 pre - S2 부위를 code 하는 DNA 서열의 대장균내 발현

최영철,김지영,유명희,한문희 ( Young Chul Choi,Ji Young Kim,Myeong Hee Yu,Moon H . Han ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.4

A DNA sequence involving pre-S2 region of Hepatitis B virus (HBV) was inserted between ompF and lacZ gene of the plasmid pORF1 to give a recombinant plasmid pHBSC20. In the plasmid pHBSC20 harboring E. coli TK1046 cells, a $quot;tribrid$quot; protein with the translation product of the insert sandwiched between OmpF and β-galactosidase was produced. The fusion protein was detected by SDS-polyacrylamide gel electrophoresis and the antigenicity of the pre-S2 region was determined by immunoblot analysis. The amount of the fusion protein was estimated as much as 1-596 of the total bacterial protein by densitometric scanning of the Coomassie blue-stained gel. Thus, bacteria transformed with pHBSC20 can provide abundant source of pre-S2-β-galactosidase fusion protein in which the pre-S2 region may be useful for the production of HBV vaccine.

간염 B 형 바이러스 표면항원 대장균 내 발현

최영철,김지영,유명희,한문희 ( Young Chul Choi,Ji Young Kim,Myeong Hee Yu,Moon H . Han ) 생화학분자생물학회 1986 BMB Reports Vol.19 No.4

Three different HBsAg sequences encoding for S region, pre-S2 plus S region, and pre-S2 fused with a portion of β-galactosidase have been expressed in E. coli under the control of lac promoter. When the S region containing HBsAg genes were expressed constitutively in E. coli HB101, the growth inhibition of recombinant cells was observed. However, the constitutive expression of pre-S2 fused with apeptide (N-terminal portion of β-galactosidase) was not detrimental to the E. coli HB101 cells. The antigenicity of the hybrid pre-S2 protein seems to be conformation-independent: treatment of 2-mercaptoethanol or 2-mercaptoethanol/SDS did not abolish the antigenicity.

STS 학습 프로그램 적용이 실업계 고등학생들의 과학 성취도 및 태도에 미치는 효과

최영철,윤일희,Choi, Young-Chul,Yoon, Ill-Hee 한국지구과학회 2001 한국지구과학회지 Vol.22 No.4

이 연구의 목적은 STS 학습 프로그램의 적용이 실업계 고등학생의 과학과 관련된 태도에 미치는 효과를 찾는 것이다. 이를 위하여 고등학교 ‘공통 과학’ 중 지구과학 영역을 선택하였고, STS 학습 프로그램을 1년 간 경북 구미시의 실업고등학교 2학년 여학생 6개반(318명)을 대상으로 하여 교수-학습에 적용하였다. 이런 과정에서 STS 학습 프로그램에 필요한 학습 보조 자료들도 개발하여 활용하였다. 그 결과 STS 학습 프로그램의 적용 후가 적용 전보다는 학생들의 과학 성취도 및 태도에 긍정적인 효과를 나타낸 것으로 나타났다. The purpose of this study is to investigate the effects of the STS program on vocational high school students' science-related attitudes. In order to examine, we selected the content of instructions at the Earth Science content in the "Common Science" and applied the STS program for one year to the second grade students of six classes (N = 318) in a girls high school located in Kumi City, Kyungpook, Korea. For this study, we developed the instructional methods and then applied to the STS program. The result of this study revealed that the STS program on students' science achievement and attitudes were effective.

Expression of Hepatitis B Virus Surface Antigen Gene in E. coli

최영철,김지영,유명희,한문희,Choi, Young-Chul,Kim, Ji-Young,Yu, Myeong-Hee,Han, Moon-H. 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.4

간염 B형 바이러스의 표면항원 유전자중에서 S부위, pre-S2와 S부위 그리고 $\beta$-galactosidase의 일부와 연결된 pre-S2 부위를 포함하는 세가지의 유전자 서열들을 lac promoter의 조절하에 대장균 내에서 발현시켰다. S부위를 포함하는 HBsAg 유전자가 E. coli HB101균주 내에서 지속적으로 발현될 경우에는 재조합 균주의 성장이 심하게 억제되었다. 그러나, $\alpha$-peptide ($\beta$-galactosidase의 N-말단부위)와 연결된 pre-S2 부위의 지속적인 발현은 E. coli HB101균주의 성장에 해로운 영향을 미치지 않았다. 또한, pre-S2 융합단백질의 항원성은 conformation과 무관한 것으로 나타났다. 2-mercaptoethanol 또는 2-mercaptoethanol/SDS의 처리후에도 항원성이 보존되었다. Three different HBsAg sequences encoding for S region, pre-S2 plus S region, and pre-S2 fused with a portion of $\beta$-galactosidase have been expressed in E. coli under the control of lac promoter. When the S region containing HBsAg genes were expressed constitutively in E. coli HB101, the growth inhibition of recombinant cells was observed. However, the constitutive expression of pre-S2 fused with $\alpha$-peptide (N-terminal portion of $\beta$-galactosidase) was not detrimental to the E. coli HB101 cells. The antigenicity of the hybrid pre-S2 protein seems to be conformation-independent: treatment of 2-mercaptoethanol or 2-mercaptoethanol/SDS did not abolish the antigenicity.

대장균에서 융합단백질의 대량생산을 위한 베타갈락토시다제 폴리펩티드 길이의 최적화

최영철,이상철,한문희,유명희 ( Young Chul Choi,Sang Chul Lee,Moon H . Han,Myeong Hee Y ) 생화학분자생물학회 1988 BMB Reports Vol.21 No.1

Plasmid vectors for cloning and directing the synthesis of large amounts of fusion proteins in E. coli were constructed. These plasmids carried tac promoter, the coding sequence of amino terminal portion of β-galactosidase, and the polylinker region at the 3`-end of truncated lacZ. When E. coli MC1061 cells were transformed with these plasmids, 25-60% of the total cellular proteins were represented by the lacZ polypeptides. The overproduced proteins formed inclusion bodies inside the cells. When the amounts of recombinant proteins produced by these plasmids were examined, the higher yield was observed with those of shorter lacZ polypeptides. The length of recombinant polypeptide with the maximum yield (60%) was about 280 amino acids long. However, when the length of β-galactosidase decreased to 200 residues as in pCT30 vector, the yield of recombinant proteins decreased dramatically. When the length of polypeptide increased again to 260 residues by fusing pre-S2 sequence of hepatitis B virus to lacZ sequence in pCT30, the yield of fusion polypeptides increased to over 50% of total cellular proteins.

조선후기(朝鮮後期) 감영(監營) 중심축(中心軸)의 구성(構成)과 직제별(職制別) 상관성(相關性)에 관한 연구(硏究)

최영철,박언곤,Choi, Young-Chul,Park, Eon-Kon 한국건축역사학회 1994 건축역사연구 Vol.3 No.2

The KAMYOUNG is the supreme local government office in CHOSEON dynasty. This study aims to propose the new methodology on research of local government office's prototype in CHOSEON dynasty. Especially this study is the zoning plan of the KAMYOUNG in the late CHOSEON dynasty, It is meant to present the first trial that pursue to total frame of local government in CHOSEON dynasty through the new methodology. The 18th century is the transitional era when the system of norm was deconstructed. The institution and organization of the supreme lacal government office(KAMYOUNG) was united nationwidely and at the same time its deconstruction was carried out in the 18th century. The domain of the governer has fixed in the 18th century, though the scale of 街e KAMYOUNG was enlarged. These domain expressed a strong symbolization according to hierarchy in exterior appearance of the traditional architecture. With the entire execution of a local government coming soon, the concerns about the localization have been increased rapidly. Under this time situation, as the discussion centered on the creation or change of the local self-governing community, so the concerns regarding the traditional aspects of a local government office have been increasing. Hower, without the recognition of this history, as the research of these existing historical materials has been progressed, so the misunderstanding is still left. Especially, because the government office of CHOSEON is a Confucian nation thinking much of a hierarchy based on Yae - Jae, the hierarchy of this architecture has a deep correlation with the organization of an office. The method of this study is like following: Firstly, prescribed characteristic of KAMYOUNG tough syudys of hierarchy in a local government system, human and material composition. secondly, as the changing progress of KAMYOUNG is compared and analyzed with organization of an office in the center. So characteristics of KAMYOUNG are abstracted. And finally, from this, the interpretation methodology of KAMYOUNG is revealed, with a deep correlation between specific characters of organization and architectural structurals. And also, with these methodologies, the interpretation method of the local government office and normative frame in CHOSEON dynasty are suggested.

Expression of a DNA Sequence Encoding for the Pre-S2 Region of Hepatitis B Virus in E. coli

최영철,김지영,유명희,한문희,Choi, Young-Chul,Kim, Ji-Young,Yu, Myeong-Hee,Han, Moon-H. 생화학분자생물학회 1986 한국생화학회지 Vol.19 No.4

간염 B형 바이러스의 pre-S2 부위를 포함하는 DNA 서열을 pORF1 플라스미드의 ompF와 lacZ 유전자 사이에 끼워 넣어 pHBSC20 재조합 플라스미드를 제조하였다. 플라스미드 pHBSC20를 갖는 E. coli TK1046 균주내에서 OmpF와 $\beta$-galactosidase사이에 pre-S2 부위를 갖는 "tribrid" 단백질이 생성되었다. 이 융합단백질은 SDS-polyacrylamide gel 전기영동에 의해 확인되었고 pre-S2 부위의 항원성은 immunoblot 분석방법에 의해 확인되었다. 융합단백질의 양은 Coomassie blue로 염색한 gel을 densitometer로 측정한 결과 전체 박테리아 단백질중 1-5%를 차지하는 것으로 나타났다. 따라서 pHBSC20 플라스미드를 갖는 박테리아는 pre-S2-$\beta$-galactosidase 융합 단백질을 다량 만들어 낼 수 있으며 이 중에서 pre-S2 부위는 HBV 백신을 생산하는데 유용할 것으로 보인다. A DNA sequence involving pre-S2 region of Hepatitis B virus (HBV) was inserted between ompF and lacZ gene of the plasmid pORF1 to give a recombinant plasmid pHBSC20. In the plasmid pHBSC20 harboring E. coli TK1046 cells, a "tribrid" protein with the translation product of the insert sandwiched between OmpF and $\beta$-galactosidase was produced. The fusion protein was detected by SDS-polyacrylamide gel electrophoresis and the antigenicity of the pre-S2 region was determined by immunoblot analysis. The amount of the fusion protein was estimated as much as 1-5% of the total bacterial protein by densitometric scanning of the Coomassie blue-stained gel. Thus, bacteria transformed with pHBSC20 can provide abundant source of pre-S2-$\beta$-galactosidase fusion protein in which the pre-S2 region may be useful for the production of HBV vaccine.

농업환경 평가를 위한 지표곤충 선발

최영철,박해철,김종길,심하식,권오석,Choi Young-Cheol,Park Hae-Chul,Kim Jong-Gill,Sim Ha-Sik,Kwon Oh-Seok 한국응용곤충학회 2004 한국응용곤충학회지 Vol.43 No.4

농업환경 평가를 위한 지표곤충을 선발하기 위해 2001년부터 2003년까지 홍천군 남면 양덕원리(홍천A), 홍천군 남면 명동리(홍천B)와 양평군 단월면 덕수리(양평)에서 농경지 주변에 서식하는 곤충을 채집, 동정하고 자연환경을 조사하였다. 시험지역의 벼 재배특성을 조사한 결과, 홍천A 지역은 농약을 년 2-3회 살포하고, 화학비료의 시비 등 일반관행 농법으로 이루어 졌으며, 홍천B 지역은 오리농법을, 양평 지역은 왕우렁이 농법을 도입하여 무농약, 무시비로 재배하면서 호맥을 재배하여 녹비로 이용하였다. 각 조사지역 내에서의 식생을 조사한 결과, 세 지역 모두 바랭이(Digitaria ciliaris (Retz.) Koel.), 돌피(Echinochloa crus-galli (L.) Beauvois) 등이 우점하였으며, 서식 식물 종수는 세 지역 모두 다양한 종이 서식하였고 녹지자연도(DGN)는 홍천A 지역(1.6)보다 홍천B 지역(2.0)과 양평 지역(3.6)이 높았다. 각 조사지역에 서식하고 있는 곤충을 채집, 분류 동정한 결과, 세 지역 모두 8, 9월에 개체수가 가장 많았으며, 채집 종수는 6월부터 9월까지 30종 이상이었다. 홍천A 지역에 비해 홍천B 지역과 양평 지역에서 채집된 곤충의 종수가 더 많았으며, 딱정벌레목의 종수가 다른 목의 곤충의 종수에 비해 월등히 많았다. 세 지역의 곤충 군집분석 결과, 우점도 지수(DI)는 홍천B 지역이 0.24로 가장 낮았으나, 종다양도(H'), 풍부도(RI)와 균등도 지수(DI)는 홍천B 지역과 양평 지역이 홍천A 지역보다 높았다. 세 지역의 시기별, 종별 채집빈도 등을 종합분석한 결과, 농업생태계 환경평가를 위한 유망 지표 곤충종으로 홍천A 지역은 딱정벌레목의 등빨간먼지벌레, 홍천B 지역과 양평 지역은 남방폭탄먼지벌레와 좀방울벌레가 선발되어야 할 것으로 판단된다. To select indicator insects, the abundance of which is one of the major criteria for the evaluation of agricultural environment, was designated and monitored in the three sites (Hongcheon A, B, and Yangpyeong) from 2001 to 2003. Characteristics of rice cultivation are usual cultivation with 2 or 3 times sprayed chemical agents per year in Hongcheon A site, duck cultivation with non-sprayed chemical agent, green manure and mowing in Hongcheon B site, and pond snail with non-sprayed chemical agent, green manure and mowing in Yangpyeong site. Dominant floral species are Digitaria ciliaris (Retz.) Koel., Persicaria thunbergii (Siebold & Zuccarini) H. Gross, Echinochioa crus-galli (L.) Beauvois, and Humulus scandens (Lour.) Merr. in Hongcheon A site, Digitaria ciliaris (Retz.) Koel., Persicaria thunbergii (Siebold & Zuccarini) H. Gross, and Echinochloa crus-galli (L.) Beauvois in Hongcheon B site, and Digitaria ciliaris (Retz.) Koel., Setaria viridis (L.) Beauv., and Persicaria langiseta (De Btuyn) Kitagawa in Yangpyeong site. Degree of green naturality (DGN) was higher in Hongcheon B site (2.0) and Yangpyeong site (3.6) than in Hongcheon A site (1.6). Number of species and individual of insects collected in the three sites were abundant on August and September. The species diversity was higher in Hongcheon B and Yangpyeong than in Hongcheon A. And Coleoptera was dominant in the all sites. The dominance index (DI) was the lowest, but diversify index (H'), evenness index (E) and richness index (RI) were the highest in Hongcheon B site. Based on three results, indicator insects for the evaluation of agricultural environment were selected as Dolichus halensis in Hongeheon A site, as Pheropsophus. javanus and Polionemobius mikado in Hongcheon B and Yangpyeong sites.