Studies on nuclear transplantation in mouse embryos. I. Functional differences between maternal and paternal genomes

최상용,박충생,이효종,박희성,Choe, Sang-yong,Park, Choon-saeng,Lee, Hyo-jong,Park, Hee-sung The Korean Society of Veterinary Science 1990 大韓獸醫學會誌 Vol.30 No.2

모성 및 부성 genome의 기능을 알아보기 위하여 미세조작기법과 Sendai virus를 이용한 핵융합 기술을 이용하여 2개의 자성전핵만으로 구성된 2배체의 gynogenetic 수정란을 그리고 2개의 웅성전핵만으로 구성된 2배체의 androgenetic 수정란을 인위적으로 작출하였다. 이들의 작출효율은 biparental 수정란에서는 56%, gynogenetic 수정란에서는 50% 그리고 androgenetic 수정란에서는 56% 이었다. 이들을 체외에서 배양한 결과 gynogenetic 및 androgenetic 수정란은 2-세포기 이후에는 biparental 및 intact 수정란에 비하여 그 발달능이 매우 저조하였으나 이들 중 25% 이상이 포배까지 발달한 수 있음을 확인하였다. Gynogenetic 및 androgenetic 수정란을 동기화된 수란생쥐의 난관내에 이식하였던 바, androgenetic 수정란은 전혀 착상 되지 않았으나, gynogenetic 수정란에서는 착상이 확인되었다. 핵이식기법으로 인위조작된 2배체의 biparental 수정란으로부터 28마리의 생쥐 신생자를 생산하였다. By nuclear transplantation technology twenty eight mice have been produced after transfer of heterozygous biparental eggs. Also heterozygous gynogenetic eggs with two female pronuclei and heterozygous androgenetic eggs with two male pronuclei have been obtained by injecting a male or female pronucleus with Sendai virus into the perivitelline space of enucleated haploid zygotes at pronuclear stage. The success rate of enucleation, karyoplast injection and fusion of both the pronuclei was 80.3, 83.4 and 81.8%, respectively. The overall pronuclei fusion rates by this technique were 56, 50 and 56% in biparental, gynogenetic and androgenetic eggs, respectively. The evidence was ascertained that the gynogenetic and androgenetic eggs were also able to develop in vitro up to blastocyst stage, even though their developmental potential was greatly diminished beyond 2-cell stage. The gynogenetic eggs were able to develop in vivo up to day 10 of pregnancy, while the androgenetic eggs failed to develop in vivo during the same period.

Vitrification 방법에 의한 토끼수정란의 동결에 관한 연구

최상용,이영락,노규진,이효종,박충생,Choe, Sang-yong,Lee, Young-rak,Rho, Gyu-jin,Lee, Hyo-jong,Park, Choong-saeng 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.3

The purpose of this study was to investigate the effects of developmental stage and equilibration time on survival of rabbit embryos following freezing by vitrification. Adult New Zealand White female rabbits were superovulated with PMSG and hCG. The 8-cell stage embryos were collected from 40 to 45 hours after hCG injection by flushing oviducts with Dulbecco's phosphated buffered saline and in vitro cultured in TCM-199 containing 10% fetal calf serum(FCS). Each embryos developed in vitro to 16-cell, compact morula and blastocyst was cryopreserved and cultured following thawing to examine their developmental potential to expanded blastocyst stage in vitro. The frozen-thawed-cultured embryos were stained with Hoechst 33342, and their nuclei were counted using a fluorescence microscope. On the toxicity test of EFS solution as cryopreservation, the survival rates of 8-cell stage embryos was decreased in reverse to increasing of exposure time over 5 minutes. The post-thaw survival rates of embryos on equilibration times was significantly(P<0.05) higher for 2 min. than for 5 or 10 minutes. From morula to blastocyst of rabbit embryos was more suitable than 8-cell stage for cryopreservation by vitrification. The higher post-thaw survival rate of embryos can be achieved by keeping the cryoprotectant at $4^{\circ}C$ than at $20^{\circ}C$. The mean number of nuclei per embryo following freezing by vitrification and in vitro culture to expanded blastocyst at compacted morula and blastcyst was not significantly differ from fresh blastocyst.

실무중심 정보보안 교육을 위한 셀 기반 입체교육 모델

최상용(Sang-Yong Choi),김정삼(Jeong-Sahm Kim),이규창(Kyu-Chang Lee) 한국정보보호학회 2021 정보보호학회논문지 Vol.31 No.5

최근 정보보안의 중요성이 더욱 커지면서 대학의 정보보안 관련 학과를 포함한 민간 또는 사설 교육기관의 정보보안 관련 학과가 증가하고 있다. 이와 같은 교육기관의 커리큘럼을 분석한 결과 전공과목에 관련하여 NCS에서 언급하고 있는 직무능력과 많은 관련이 있는 것으로 보이며, 이는 직무능력별 표준화된 교육수준을 제공할 수 있는 장점이 있다. 하지만 교과목 기반의 교육과정은 교육 기관별 중복 커리큘럼, 강사의 수준에 따른 교육품질, 필요한 요소기술의 적시 교육 등에 대한 한계점이 발생할 수 있다. 본 논문에서는 대학 및 민간 또는 사설 교육기관의 교육과정을 분석하고, 한계점 해결을 위한 셀 기반 입체교육 모델을 제안한다. 제안하는 모델은 발생 가능한 한계점을 효과적으로 개선할 수 있음을 전문대학 교육과정을 기초로 검증한다. Recently, the importance of information security has become greater. For this reason, the number of departments related to information security at universities and professional educational institutions is increasing. It is analyzed that the curriculum of such educational institutions has a lot to do with the job competency mentioned in NCS in relation to major subjects. This has the advantage of providing a standardized level of education for each job competency. However from the perspective of students there may be limitations on duplicate curriculum quality of education according to the level of teachers and timely acquisition of necessary elemental skills. In this paper we analyze the curriculum of universities and professional educational institutions and propose a cell-based three-dimensional education model to address the limitations of students. We verified based on the college curriculum that the proposed model can effectively improve its limitations.

생쥐 8- 세포기 수정란의 핵이식에 의한 복제산자의 생산

최상용(S . Y . Choe),박희성(H . S . Park),이효종(H . J . Lee),박충생(C . S . Park) 한국축산학회 1992 한국축산학회지 Vol.34 No.2

The present study was carried out to develop a technology of cloning mammalian embryos using mouse embryos as a model and to produce cloned offsprings by transfer of reconstituted embryos to recipient females. Single nucleus from eight-cell embryos was transplanted into the enucleated two-cell embryos by micromanipulation and Sendai virus-mediated fusion. The fusion of nuclei with recipient cytoplasm and the development of reconsitituted embryos in vitro as well as in vivo were examined. The results obtained were summarized as follows: A total of 95(59.3%) nuclei from 20 donor embryos of 8-cell stage was injected successfully into the enucleated 2-cell recipient embryos, of which octa-. hepta-, hexa-, penta- and quadruplets were 0. 2, 3, 3 and 12 sets, respectively. The success in nuclei injection was lower(P$lt;0.05) from 8-cell donor embryos than 4-cell or 2-cell donor embryos. Among the embryos injected with nuclei a total of 81(85.2%) embryos were fused successfully with donor embryos of 8-cell stage. From donor embryos of 8-cell stage, 2 hexa-, 2 penta-, 11 quadru- and 5 triplets were fused successfully. A total of 46(39.l%) reconstituted embryos from 8-cell donor nuclei were developed in vitro to blastocysts, of which penta-, quadru- and triplets. twins and singles were 1, 2, 6, 6 and 3 sets, respectively. The development in vitro to blastocysts of the reconstituted embryos was lower (P$lt;0.05) from 8-cell donor embryos than 4- or 2-cell donor embryos. A total of 13(27.l%) cloned live youngs from transfer of cloned embryos using 8-cell donor embryos were produced, of which triplets, twins and singles were 2, 3 and 1 sets, respectively. The percentage of cloned youngs produced from transfer of cloned embryos using 8-cell donor embryos was lower(P$lt;0.05) than 4- or 2-cell donor embryos. The mean number of cloned live youngs produced per 8-cell donor embryo used for nuclei transplantation was 0.81.

성충에 처리한 IGRs계 살충제가 집파리의 산란과 불임에 미치는 영향

박정규,최상용,김종수,김두호,이흥수,Park, Chung-gyoo,Choe, Sang-young,Kim, Jong-shu,Kim, Doo-ho,Lee, Heung-su 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

Two-day old house fly adults were exposed to six insect growth regulators, flufenoxuron, teflubenzuron, triflumuron, diflubenzuron, methoxyfenozide, tebufenozide, as a feed additive (milk+5% sugar+chemical) in the laboratory for 6 days. The number of eggs deposited by the exposed-adults, viability of the eggs, and $F_1$ larval development were checked. All the IGRs tested were found to have no adverse effect on the reproduction of house fly, except methoxyfenozide (210ppm). The most effective inhibitor to egg hatch was flufenoxuron, followed by teflubenzuron, triflumuron, and diflubenzuron. Exposure to flufenoxuron (over 5ppm), teflubenzuron (over 25ppm), triflumuron (over 125ppm), and diflubenzuron (over 125ppm) reduced egg hatchability to 0 to 1.3%, but lower concentrations of these IGRs were less effective (6.3 to 46.3% egg hatchability). Almost all the larvae emerged from eggs deposited by the adults exposed to diflubenzuron (62.5ppm) and teflubenzuron (12.5ppm) failed to develop into pupae, causing total mortalities of 98% and 100%, respectively. However, two IGRs, methoxyfenozide and tebufenozide, did not inhibit egg hatch and $F_1$ larval development, except methoxyfenozide (210ppm) treatment These results suggest that these 4 IGRs may be used in the development of autosterilization system for house fly control. However, further work is required to develop delivery systems capable of transferring an effective dose to the fly under field conditions.

Studies on nuclear transplantation in mouse embryos II. Developmental potential of nuclei from embryos of different developmental stages

박충생,최상용,이효종,박희성,Park, Choong-saeng,Choe, Sang-yong,Lee, Hyo-jong,Park, Hee-sung The Korean Society of Veterinary Science 1990 大韓獸醫學會誌 Vol.30 No.4

Single nuclei from two-, four- and eight-cell mouse embryos were transplanted into enucleated two-cell embryos by micromanipulation and Sendai virus mediated fusion. The developmental potential of these reconstituted embryos in vitro and in vivo was examined. It was found that the single nuclei which were transplanted to enucleated two-cell embryos were not only able to develop to the blastocyst stage in vitro(two-cell nuclei, 76.5%; four-cell nuclei, 68.4%; eight-cell nuclei, 48.3%), but also able to develop to full term in vivo after transfer to recipient mice(two-cell nuclei, 37.1%; four-cell nuclei, 29.6%; eight-cell nuclei, 16.3%). Although the proportion of live young produced after transfer of nucler of nuclear transplant embryos which received eight-cell nuclei was significantly (p<0.05) reduced, it would be suggested that the overall efficiency in producing identical offspring is greater when eight-cell embryos were selected for nuclear donor than two- or four-cell embryos were selected. 포유동물의 초기 발생단계에서 핵의 분화와 전능성(totipotency) 을 규명하고, 수정란의 cloning technique를 개발하여 우량유전자로 조성된 개체를 복제함으로써 효과적인 종축개량 기법으로 응용하기 위하여 생쥐 수정란을 모델로 하여 미세조작기법과 Sendai virus를 이용한 핵융합기술을 이용하여 인위적으로 동일한 유전자를 가진 복제 수정란을 작출하고 이들의 작출효과, 체외발달능력 및 체내 이식후 개체발생여부 등을 조사하였다. 2-세포기, 4-세포기 및 8-세포기의 수정란으로부터 핵을 채취하여 이들을 탈핵된 2-세포기의 수정란에 이식하였을 때, 이들의 핵융합 성공율은 각각 88.6%, 87.1% 및 84.7%이었다. 나아가서 이들 핵융합된 수정란을 체외에서 96시간 배양한 결과, 2-세포기, 4-세포기 및 8-세포기의 핵이 이식된 수정란은 각각 76.5%, 68.4% 및 48.3%가 배반포로 발달하였다. 핵이식 후 체외에서 배반포로 발달된 수정란을 골라 수란생쥐에 이식하였던 바, 2-세포기의 핵이 이식된 수정란 156개 중 58개(37.1%) 가 발달하여 신생자로 생산되었으며, 4-세포기의 핵아 이식된 수정란 135개 중 40개(29.6%)가, 그리고 8-세포기의 핵이 이식된 92개의 수정란 중 15개(16.3%)가 신생자로 생산되었다.

PF_Ring을 이용한 대용량 트래픽 처리 시스템

이달원(Dalwon Lee),고대식(Dae-Sik Ko),김동환(Donghwan Kim),최상용(Sang-Yong Choi) 한국정보기술학회 2020 한국정보기술학회논문지 Vol.18 No.2

The advances in IoT (Internet of Things) and Big Data technologies have enabled various devices to work together, also causing the amount of traffic between systems to increase significantly. In these situations, real-time processing of large traffic is critical in order to effectively control the operational status, failure status, and security threat of the system and network. In this paper, a system using PF_Ring is proposed which is capable of extracting meaningful data from high-capacity traffic of 5Gbps in real-time. The proposed system installed PF_Ring in the Linux system kernel, periodically executed PF_Ring using the Collector Handler to collect traffic and read the data through Packet_preprocessor to operate in parallel. The experiment results have confirmed that the proposed system is able to fully handle the entire traffic within one minute, which is the next pre-processing cycle, with the loss rate of 0% in processing the 5Gbps collected packets and pre-processing time of approximately 47 seconds.

소의 조기임신진단 kit의 개발 1. Progesterone의 항체생산(抗體生産) 및 항(抗) BSA항체(抗體)의 제거

강정부,이효종,최상용,Kang, Chung-boo,Lee, Hyo-jong,Choe, Sang-yong 대한수의학회 1991 大韓獸醫學會誌 Vol.31 No.2

Most progesterone enzyme immunoassays(EIA) are used liquid phase double-antibody separation. These methods consume considerable time and reagents because of the requirements for several washing and centrifugation steps involving the reactants. Because of there several problems, we were prompted to develop an effective EIA system by the use of higher titer of progesterone antiserum free of anti-bovine serum albumin antibodies (anti-BSA). The results obtained were as follows. 1. The antibody of progesterone antiserum was high as $1.5{\times}10^5$. 2. Percent activity bound of progesterone antiserum was about 77 at a dilution to $5{\times}10^3$ times. 3. Progesterone antiserum was contained a large amount of anti-BSA antibodies. 4. The anti-BSA was completely absorbed by using of polymerised BSA. 5. The molecular weight of albumin polymer (polymerised BSA) obtained by using 2.5% glut. araldehyde was $5{\times}10^5$.

결장 및 직장에서 발생한 원발성 림프종

이상달(Sang Dal Lee),권도성(Do Seong Kwon),목영재(Young Jae Mok),최상용(Sang Yong Choi),문홍영(Hong Young Moon) 대한외과학회 1996 Annals of Surgical Treatment and Research Vol.51 No.2

신생아기 장폐색증에 대한 임상적 고찰

이효봉(Hoe Bong Lee),최상용(Sang Yong Choi),박신희(Shin Hee Park),김진승(Chin Seung Kim) 대한외과학회 1999 Annals of Surgical Treatment and Research(ASRT) Vol.56 No.3