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태국칡(Pueraria mirifica)으로부터 norsesquiterpene의 분리 및 동정
권정화 ( Jung Hwa Kwon ),조진경 ( Jin Gyeong Cho ),박희정 ( Hee Jung Park ),허규원 ( Gyu Won Huh ),방면호 ( Myun Ho Bang ),한민우 ( Min Woo Han ),오창환 ( Chang Hwan Oh ),고성권 ( Sung Kwon Ko ),조수열 ( Soo Yeul Cho ),최갑용 ( Ka 한국응용생명화학회(구 한국농화학회) 2014 Journal of Applied Biological Chemistry (J. Appl. Vol.57 No.4
Pueraria mirifica 뿌리를 실온에서 70% EtOH 수용액으로 추출하고 이 추출물을 EtOAc 분획, n-BuOH 분획, H2O 분획으로 나누었다. EtOAc 분획에 대하여 silica gel, octadecyl silica, 및 Sephadex LH-20 c.c.를 반복 실시하여 4종의 화합물을 분리, 정제하였다. Nuclear magnetic resonance, infrare, 및 mass spectrometry의 spectroscopic data를 해석하여, 화합물 1-4를 각각 megastigm-5-en-3,9-diol, linarionoside B, 3,5,6,9-tetrahydroxymegastigm-7-ene 및 3,4,9-trihydroxymegastigma-5,7-diene으로 구조를 결정하였다. 화합물 1-4 모두 P. mirifica에서는 이번에 처음으로 분리된 화합물이다. The roots of Pueraria mirifica were extracted with 70% aqueous ethyl alcohol and partitioned into ethyl acetate (EtOAc), n-butyl alcohol (BuOH), and H2O fractions, successively. From the EtOAc fraction, four norsesquiterpenes were isolated through the repeated silica gel, octadecyl silica gel and Sephadex LH-20 column chromatographies. On the basis of physicochemical and spectroscopic data including nuclear magnetic resonance (NMR), mass spectrometry, and infrared spectroscopy, the chemical structures were identified as megastigm-5-en-3,9-diol (1), linarionoside B (2), 3,5,6,9-tetrahydroxymegastigm-7-ene (3) and 3,4,9-trihydroxymegastigma-5,7-diene (4). Especially, the configuration of the anomer hydroxyl group was determined as a from the coupling constants of the anomer proton (J =8.0 Hz) in the 1H-NMR spectrum. These compounds were isolated for the first time from the roots of P. mirifica in this study.