Diode Laser 조사가 가토경골의 골재형성에 미치는 영향에 관한 연구

강봉기(Bong Ki Kang),천재식(Gae Sig Chun) 대한구강악안면병리학회 2010 대한구강악안면병리학회지 Vol.34 No.6

Currently, Low-level laser therapy (LLLT) is widely used in medicine and dentistry. It has been suggested that LLLT may be beneficial in management of many different medical conditions, including pain, wound healing and nerve injury. Stimulation with LLLT can enhance bone repair as reported in experimental studies on bone defects and fracture healing also. As far as hard tissue is concerned, the biostimulating effect of laser has been demonstrated more rapid healing of tibial bone fracture in mice. This study was performed to compare new bone formation between with LLLT and without LLLT. Two cylinder implants(5mm diameter, 5mm length) were implanted on rabbit's tibia. LLLT was done to one implant with 632mm diode laser. And than Rabbit's were sacrificed after 2nd, 4th, 6th, 8th weeks after implantation. Bone with implant were removed and fixed with 10% formaline. Undecalfied sample were prepared after spurr low resin embedding. Sample were grind and polished to 100㎛. The results were as follows. The amount of trabeclue of experimental group were superior to that of control group from 2 weeks to 8 weeks. There were no difference in arrangement of trabecule between two groups. Bone implant contact were significantly increased at 4th weeks in experimental group. The number of ostecytes in trabecule were different at 4th weeks experimental group. Osteone were appeared dominantly at 6th weeks experimental group, while at 8th weeks experimental group were superior than control group. LLLT group showed increased amount of trabecule, bone implant contact, number of osteocytes & osteone. It suggested that LLLT might be increase the bone formation rate and accelerate the bone formation time.

발음시 파장 및 기간의 변화에 관한 연구

千在植,鄭世賢 단국대학교 1994 論文集 Vol.28 No.-

Sound is the sensation produced when longitudinal variations of molecules in the external environment. ie, altenate phases of condensation and rarefaction of the molecules, strike the tympanic membrane. The purpose of speaking is communication between humans. It is very important for social life to speak and hear exactly. It there were problems to speak, it is very unconvenient for social life psychologically. The purpose of this experiment was to analyse the frequency and duration of pronunciation. Each 20 male and female who were living in Seoul and speaking standard Korean, had participated in this experiment. Each 10 male and female, who were wearing dentures and adapted at least over 1 years, had participated in this experiment also. The data were taken and analysed by Philips PM 3350A Storage Oscilloscope which had 68008 up microprocessor IEEE-488 interface. The results were as follows 1. High pitches were appeared in early stage of pronunciation in plosive sounds(ㅂ, ㅋ, ㅌ, ㅍ)and Y system vowel diphthorgs. 2. Condensation waves were seen in later stage of pronunciation in frictional sounds(ㅅ,ㅎ) and nasal sound(ㄴ, ㅁ). 3. the average data of frequency and duration were as follows ◁ 표 삽입 ▷ (원문을 참조하세요) 4. There were special pitches such as 111, 181, 200, 250, 333, 400, 500, 1000, 2000 Hz in all subjects

정상인 악골내 삽입된 I.M.Z. 매식체의 골융합에 관한 주사전자현미경적 연구

주경환,천재식,이종헌 단국대학교 치의학연구소 1993 논문집 Vol.5 No.-

The purpose of this study was to observe morphologically osseointegration and change about bone and peripheral tissue in IMZ implants on 3 and 9 month in normal human mandible. 4 IMZ implants with 3.3mm in diameter and 10mm in length were implanted in normal human mandible. For scanning electron microscopic study in 3 and 9 month, IMZs were explanted by trephine bur, washed with normal saline, fixed with 2.5% glutaraldehyde for 3 days, postifixed with 1% OsO_4, dehydrated gradually with ethyl alchol, changed with isoamyl acetate, and dried with CO_2 critical point dryer. The specimens were gold-plated and examined with scanning electronmicroscope. The obtained results were as follows. 1. There were moderately mineralized trabecular in vent of IMZ, irregular directions of collagen fibers, fibroblasts, osteocytes with prominent process surrounded by moderately mineralized bone in scanning electron microscopic study after 3month of post operation tissues. 2. Scanning electron microscopic study of 3 month showed rarely inflammatory cell infiltration among IMZ, bone tissue, and collagen fiber, disclosing many blood vessels in less mineralized osteoid containing RBCs, fibrin, and rare leukocytes. 3. Scanning electron microscopic study of 9 month showed mainly mineralized, irregular trabecular attached to vent of IMZ, and osteocytes with promint processes in lacuna surrounded by mineralized bone. 4. Scanning electronmicroscopic study of 9 month showed rarely inflammatory cell infiltration between bone and IMZ, and more decreased number of blood vessel than that of 3 month.

Capsaicinoids가 삼차신경감각핵의 신경전달물질 유리에 미치는 영향

안명석,이일규,천재식 단국대학교 치의학연구소 1994 논문집 Vol.6 No.2

Excitatory amino acids(EAA) neurotransmitters, glutamate and aspartate, play a role in transmission of primary afferents of orofacial pain in trigeminal spinal sensory nucleus. This experiment was performed to study the role of capsaicin, its derivative and analogue on release of glutamate and aspartate from trigeminal spinal sensory nucleus in slice of brainstem. Horizontal slice of caudal brainstem of rat was prepared and perfused with modified Krebs-Ringer solution in brain slice chamber. Release of EAAs was induced by capsaicin(10μM), KR-25018(10μM) and shogaol(10μM), which was added to perfusion solution to observe the changes in EAAs release and effect of ruthenium red and capsazepine on the capsaicinoids. And then EAAs being determined by HPLC. Release of glutamate and aspartate from horizontal slice of caudal brainstem was increased by capsaicin and KR-25018. But shogaol had not affected on release of EAA. Effect of capsaicin and KR-25018 was partially blocked by capsaicin antagonists, ruthenium red and capsazepine in vitro.

가토의 경골에 매식된 IMZ 매식체 주위의 신생골 형성에 관한 형광현미경적 연구

황영구,이일규,이종헌,천재식 단국대학교 치의학연구소 1994 논문집 Vol.6 No.2

The purpose of this study was to observe morphologically remodelling and osseintegration of undecalcified bone and peripheral tissue attached to IMZ implants with 3.3㎜ in diameter and 6㎜ in length, being emdedded in rabbit's tibia. After intraperitoneal injection of 25mg/kg oxytetracyclin every weeks the explanted IMZ implants associated with bone tissue were fixed with 10% neutral formalin, dehydrated, and embedded with Spurr low viscosity after 3,6,8,10 weeks. After the specimen was cut by 500um with slow diamond wheel saw, these were grinded up to 100um with grinder, and examined with fluorescent microscopy. The obtained results were as follows. 1. There was TC-labelled low density bone mainly surrounding IMZ implant after 3 weeks of implantation. 2. There was TC-labelled, relative mineralized bone in close proximity to IMZ implant demarcated by compact bone after 6 weeks of implantation. 3. There was TC-labelled mineralized bone with osteocytes slightly demarcated by compact bone after 8 weeks of implantation. 4. There were more osteocytes adjacent to IMZ implant than those of 8 weeks, and slight TC-labelled line partially surrounding implant after 10 weeks of implantation.

가토의 경골에 매식된 I.M.Z.매식체 주위 골조직변화에 관한 반사전자현미경적 연구

김영규,이일규,이종헌,천재식 단국대학교 치의학연구소 1994 논문집 Vol.6 No.2

The purpose of this study was to observe the change of bone and peripheral tissue around IMZ implants on 3, 6, 8, 10, and 12 weeks in rabbit tibia morphologically. TPS-IMZ extraoral implants with 3.3㎜ in diameter and 6㎜ in length were implanted in rabbit tibia. For reflection electronmicroscopic study, IMZ implants were explanted, fixed with 10% neutral formalin, coated with Au-Pd, and examined with reflection electonmicroscopy. The obtained results were as follows. 1. Irregular osteoid like tissue in the upper portion of IMZ implant and less mineralized bone tissue surrounding implant, which was well demarcated from old bone tissue after 3 week implantation. 2. There were irregular trabecular pattern of less mineralized bone containing numerous osteocytes in the upper portion of IMZ implant, showing well demarcated line from old bone tissue and Haversian canals attached to implant after 6 week implantation. 3. There was increased mineralized in the upper portion of IMZ implant after 8, 10 week implantation. Various density of mineralization between adjacent bone tissue around implant and old bone was decreased but osteocytes in newly formed bone tissue were more increased than those of old tissue. 4. There was poorly demarcated line between newly formed bone tissue and old bone and newly formed bone was intermingled with old one and less mineralized bone tissue around Haversian canals after 12 week implantation.

정상인 악골에서 IMZ매식체 내부 골조직 형성에 관한 조직계측학적 연구

배성호,이일규,이종헌,천재식 단국대학교 치의학연구소 1994 논문집 Vol.6 No.2

The purpose of this study was to observe bone formation histomorphometrically, and bone, and peipheral tissue attached to IMZ implants which were 3.3㎜ in diameter and 10.0㎜ in length, being emdedded in the normal human mandible. After 1, 3 and 9 month IMZ implants explanted by trephine bur were fixed with 10% neutral formalin, dehydrated, and embedded with Spurr low viscosity. After the specimen was cut by 500㎛ with slow diamond wheel saw and these were grinded up to 100 ㎛ with grinder, stained with toluidine blue and Villanueva's method, and examined with light microscope in IMZ vent. The obtained results were as follows. 1. Histopathologic findings of 1 month showed slight new bone formation attached to implant, which was mainly surrounded by fibrous collagenous tissue. 2. Toluidine blue of 3 month showed strong positivity of outer lamellar pattern and osteocytes in high density bone and central direction of trabecular bone, but weak positivity of inner lamellar pattern. 9 month showed increased lamellar pattern around IMZ implants, more denser in bone adjacent to IMZ implant. 3. Villanueva of 3 month showed IMZ partially surrounded by lowdensity bone as light red and brown, connective tissue, osteocytes, and lacuna as red. 9 month showed IMZ implant mainly surounded by high density bone as heavy brown, and increased osteocytes in lacuna. 4. IMZ implant width of 1 month was 37.5±2.0%, bone width 8.5±1.0%, soft tissue width 54.0±4.0%. IMZ implant width of 3 month was 38.0±3.0%, bone width 43.2±2.0%, soft tissue width 19.2±4.0%. IMZ implant width of 9 month was 37.0±3.0%, bone width 56.0±2.0%, and soft tissue width 7.0±2.0%. 5. A direct bone contact of 1 month was 12.3±2.0%, and a direct soft tissue contact was 87.8±5.0%. A direct bone contact of 3 month was 46.9±4.0%, and a direct soft tissue contact was 53.1±4.0%. A direct bone contact of 9 month was 56.6±5.1%, and a direct soft tissue contact was 43.4±5.1%. 6. There was active bone formation in close proximity to IMZ implant from 1 to 3 month. The change from soft tissue to bone tissue in central portion of the vent was more active than on the interface of IMZ implant.

IMZ매식체와 주위 골조직 사이의 골융합에 관한 실험적 연구

김천식,이일규,이종헌,천재식 단국대학교 치의학연구소 1994 논문집 Vol.6 No.2

The purpose of this study was to observe morphologically remodelling and osseointegration of undecalcified bone, and peipheral tissue attached to IMZ implants with 3.3㎜ in diameter and 6.0㎜ in length, being emdedded in rabbit tibia. After 3, 6, 8, 12 weeks, IMZ impants associated with bone tissue were explanted, fixed with 10% neutral formalin, dehydrated, and embedded with Spurr low viscosity resin. After the specimen was cut by 500㎛ with slow diamond wheel saw, these were grinded to 100㎛ with grinder, stained with toluidine blue and Villanueva's method, and examined with lgiht microscope. The results obtained were as follows. 1. Histopathologic findings of 3 weeks showed IMZ implants mainly surrounded by low density bone, and regular directions of collagen fibers but no inflammatory cell infiltration. Findings of 6 weeks showed new bone well demarcated by compact bone, and findings of 8 weeks showed osteocytes with processes adjacent to implant, and findings of 10 weeks showed high density bone surrounding IMZ implant less well demarcated by compact bone. 2. Toluidine blue of 3 weeks showed strong positivity of low density bone and collagen fibers. Findings of 8 weeks showed high density bone containing increased lamellar and osteocytes with processes. Findings of 10 weeks showed osteocytes among plasma parallel to implant. 3. Villanueva of 3 weeks showed IMZ implants partically surrounded by low density bone as light red and brown. Findings 6 weeks showed osteocytes, lacuna, canaliculi, and Harversian canal as red. Findings 8 weeks showed implants mainly surounded by high density bone as brown, and more increased osteocytes. But Findings 10 weeks showed implants surrounded by less denser bone than compact bone.