Epinephrine의 dose-response에 미치는 α- 및 β-receptor blocking agents의 영향(影響)

천연숙(Yun Sook Cheon),전보권(Bo Gwun Chun),신만련(Man Ryun Shin) 대한약리학회 1977 대한약리학잡지 Vol.13 No.2

Adrenergic receptors are now classified into alpha type and beta type These adrenergic receptors are distributed in various tissue in different patterns. Therefore, the adrenergic response of a certain tissue may be different from those of the other tissues, and such differences may exist among various species of animals. In this paper, the authors attempt to reevaluate the effect of epinephrine on the isolated atria, aortic strips, and vas deferenses of rabbits preincubated with alpha receptor blockades (ergotamine and dibenamine) and beta receptor blockades (propranolol and dichloroisoproterenol) in Locke-Ringer bathing medium. The results obtained were summarized as follows; 1) The dose dependent responses of isolated atria to epinephrine were significantly inhibited by propranolol and dichloroisoproterenol, and slightly inhibited by dibenamine, but not affected by ergotamine. 2) The dose dependent responses of excised aortic strips to epinephrine were significantly inhibited by ergotamine and dibenamine, but the responses were slightly potentiated by propranolol, and significantly by dichloroisoproterenol. 3) The dose dependent responses of isolated vas deferenses to epinephrine were significantly inhibited by ergotamine and dibenamine, but slightly potentiated by propranolol and dichloroisoproterenol.

Fluoride가 적출장기(摘出臟器)의 Catecholamine 유리(遊離) 및 Monoamine Oxidase 활성도(活性度)에 미치는 영향(影響)

천연숙(Cheon, Yun-Sook),김성숙(Kim, Sung-Sook),이경희(Lee, Kyung-Hee),신경철(Shin, Kyung-Chul) 대한약리학회 1972 대한약리학잡지 Vol.8 No.2

Fluorides were supposed to exert a stimulatory action on the catecholamine release. In this study, the authors attempted to investigate the action of sodium fluoride on the catecholamine release from the isolated perfused cow adrenal gland and rat heart. And also the inhibitory effect of sodium fluoride on the monoamine oxidase activity in rat heart and liver mitochondria was investigated. The monoamine oxidase activity was measured by the conversion of benzylamine to benzaldehyde. The results obtained were follows; 1. Sodium fluoride stimulated the release of catecholamine from the isolated perfused cow adrenal gland and rat heart. 2. Sodium fluoride inhibited the rat heart and liver mitochondrial monoamine oxidase activity.

$CC1_4$의 간장독작용(肝臟毒作用)에 미치는 Thyroxine의 영향(影響)

홍기성,천연숙,Hong, Ki-Sung,Cheon, Yun-Sook 대한약리학회 1980 대한약리학잡지 Vol.16 No.2

Calvert et al. formulated the hypothesis that carbon tetrachloride ($CCl_4$) acted on the central nervous system to produce and intensify sympathetic discharge which resulted in anoxic necrosis of the liver. Recknagel suggested that the essential feature of $CCl_4$ hepatotoxicity depended on the cleavage of it to $CCl_3$(free radical) and the peroxidative decomposition of cytoplasmic membrane structural lipids. And there are many reports which show the increase of adrenergic activity in hyperthyroidism. In this paper, the influence of thyroxine on the hepatotexicity of carbon tetrachloride was investigated in mice. The results obtained were summarized as follows; 1) Hepatic total lipid and lipid peroxide contents were slightly decreased by L-sodium thyroxine injection(4mg/kg/day for 4days or 6days), but hepatic glycogen content was significantly decreased. 2) Hepatic total lipid and lipid peroxide contents and serum lactic dehydrogenase activity were significantly increased by $CCl_4$ (4 ml/kg single dose or triple dose: 4ml/kg/day for 3days), but hepatic glycogen content was significantly decreased. 3) The increase of hepatic total lipid and lipid peroxide contents and serum lactic dehydrogenase activity induced by $CCl_4$ were significantly inhitited by the pretreatment of thyroxine. 4) The decrease of hepatic glycogen induced by $CCl_4$ was not affected by the pretreatment of thyroxine.

Phenytoin과 Phenobarbital이 뇌내(腦內) Catecholamine함량(含量)과 혈장(血漿) Corticosterone치(値) 변동(變動)에 미치는 영향(影響)

김남헌,천연숙,Kim, Nam-Heon,Cheon, Yun-Sook 대한약리학회 1982 대한약리학잡지 Vol.18 No.2

In this paper, the influence of phenytoin and phenobarbical on the changes of brain norepinephrine(NE) content, plasma corticosterone and blood sugar level in mice were studied. The results obtained were summarized as follows: 1) Phenytoin(50 mg/kg) increased the brain NE content but phenobarbital(50 mg/kg) did not affect. The increase of the brain NE content induced phenytoin was potentiated by phenobarbital pretreatment. 2) Phenytoin(25 mg/kg, 50 mg/kg) markedly increased the level of plasma corticosterone but phenobarbital did not affect. The increase of the plasma corticosterone induced by phenytoin was inhibited by phenobarbital pretreatment. 3) Phenytoin(50 mg/kg) markedly increased the blood sugar level but phenobarbital did not affect. The increase of the blood sugar induced by phenytoin was not affected by phenobarbital pretreatment.

Ketamine의 뇌(腦) Norepinephrine함량(含量)과 혈장(血漿) Corticosterone치(値) 변동(變動)에 미치는 Reserpine과 Cocaine의 영향(影響)

김양숙(Yang Sook Kim),김학열( Hak Ryul Kim),천연숙(Yun Sook Cheon) 대한약리학회 1983 대한약리학잡지 Vol.19 No.1

Ketamine이 백서(白鼠)의 전뇌(全腦)와 심장조직중(心臟組織中)의 NE함량(含量)과 혈장(血漿) corticosterone치(値) 변동(變動)에 미치는 영향(影響)을 관찰(觀察)하고 아울러 reserpine 및 cocaine을 전처치(前處置)하고 ketamine의 효과(效果)에 미치는 영향(影響)을 비교(比較) 검토(檢討)하고 다음과 같은 결론(結論)을 얻었다. 1) 정상(正常) mouse에 ketamine 5mg, 10mg, 20mg 및 40m1/kg를 각각(各各) 투여(投與)한 후(後) 30분(分)에 측정(測定)한 혈장(血漿) corticosterone치(値)는 약물(藥物)의 투여양(投與量) 증가(增加)에 따라서 점차적으로 상승(上昇)되며, 20mg/kg와 40mg/kg에서는 더욱 현저(顯著)하였다. 2) ketamine은 투여후(投與後) 30분(分) 및 60분(分)에 전뇌(全腦)와 심장조직중(心臟組織中)의 NE함량(含量)을 감소(減少)시켰다. 3) ketamine에 의(依)한 혈장(血漿) corticosterone치(値)는 reserpine 36시간(時間) 전처치(前處置)로 감소(減少)되는 경향(傾向)을 보였고, cocaine 15분(分) 전처치(前處置)로는 별(別) 변화(變化)를 볼 수 없었다. 4) ketamine에 의(依)한 전뇌(全腦)와 심장조직중(心臟組織中)의 NE함량(含量)은 reserpine 전처치(前處置)로 감소(減少)되는 경향(傾向)을 보였고, cocaine 전처치(前處置)로는 증가(增加)되는 경향(傾向)을 보였다. The influences of reserpine and cocaine on the changes of brain ana cardiac norepinephrine (NE) contents and plasma corticosterone level induced by ketamine in mice were studied. The results obtained were summarized as follow: 1) Various doses(5 mg, 10 mg, 20 mg and 40 mg/kg) of ketamine gradually increased the plasma corticosterone levels in 30 minutes, especially 20 mg/kg and 40 mg/kg dose treatments markedly increased the levels. 2) Brain and cardiac NE contents were decreased in 30 minutes and 60 minutes after ketamine treatment. 3) The plasma corticosterone level induced by ketamine was decreased by reserpine pretrement(36 hr.) but not affected by cocaine pretreatment(15 min.). 4) Brain and cardiac NE contents induced by ketamine were decreased by reserpine pretreatment, but increased by cocaine pretreatment.

Clondine의 과혈당작용(過血糖作用)에 미치는 교감신경차단약물(交感神經遮斷藥物)의 영향(影響)

김수경(Soo Kyung Kim),천연숙(Yun Sook Cheon),신만련(Man Ryun Shin) 대한약리학회 1982 대한약리학잡지 Vol.18 No.1

In this paper, the influences of adrenergic blockades; propranolol and phenoxybenzamine on the changes of hyperglycemic action, hepatic glycogen content, and brain norepinephrine (NE) content induced by clonidine were investigated in the male mice. The results obtained were summarized as follows: 1) Blood glucose level was significantly increased by clonidine (30μg/kg). The increase of blood glucose level induced by clonidine was not affect by the propranolol (10mg/kg) pretreatment, but significantly inhibited by the phenoxybenzamine (10mg/kg) pretreatment. 2) Hepatic glycogen content was moderately inhibited by clonidine. The decrease of hepatic glycogen content induced by clonidine was not affected by the propranolol and phenoxybenzamine pretreatment. 3) Brain NE content was significantly increased in 30 minutes and 60 minutes after clonidine treatment. The increase of brain NE content induced by clonidine was significantly inhibited by the phenoxybenzamine pretreatment. The increase of train NE content induced in 90 minutes and 120 minutes after clonidine treatment was more markedly increased by the propranolol pretreatment.

CC1₄의 간장독작용(肝臟毒作用)에 미치는 Thyroxine의 영향(影響)

홍기성(Ki Sung Hong),천연숙(Yun Sook Cheon) 대한약리학회 1980 대한약리학잡지 Vol.16 No.2

Calvert et al. formulated the hypothesis that carbon tetrachloride (CCl₄) acted on the central nervous system to produce and intensify sympathetic discharge which resulted in anoxic necrosis of the liver. Recknagel suggested that the essential feature of CCl₄ hepatotoxicity depended on the cleavage of it to CCl₃(free radical) and the peroxidative decomposition of cytoplasmic membrane structural lipids. And there are many reports which show the increase of adrenergic activity in hyperthyroidism. In this paper, the influence of thyroxine on the hepatotexicity of carbon tetrachloride was investigated in mice. The results obtained were summarized as follows; 1) Hepatic total lipid and lipid peroxide contents were slightly decreased by L-sodium thyroxine injection(4mg/kg/day for 4days or 6days), but hepatic glycogen content was significantly decreased. 2) Hepatic total lipid and lipid peroxide contents and serum lactic dehydrogenase activity were significantly increased by CCl₄ (4 ml/kg single dose or triple dose: 4ml/kg/day for 3days), but hepatic glycogen content was significantly decreased. 3) The increase of hepatic total lipid and lipid peroxide contents and serum lactic dehydrogenase activity induced by CCl₄ were significantly inhitited by the pretreatment of thyroxine. 4) The decrease of hepatic glycogen induced by CCl₄ was not affected by the pretreatment of thyroxine.

Guanethidine이 白鼠摘出心臟의 Catecholamine 遊離에 미치는 效果

千然淑 고려대학교 의과대학 1975 고려대 의대 잡지 Vol.12 No.2

The isolated heart of matured rat in the perfusion experiments respond to continuous infusion of guanethidine with an increase in heart rate. This agent appear to increase heart rate by releasing endogenous norepinephrine, since depletion of cardiac norepinephrine store abolishes the action. In the present studies, to inverstigate the role of guanethidine on the release of catecholamine, the author observed the influence of guanethidine on the variations of catecholamine contents in the myocardium and perfusate from the perfusion experiment of isolated rat heart. Perfusions were carried out with varying concentration(1㎎, 5㎎ and 10㎎ per 1L of Tyrode solution) of guanethidine for 1 hour, 2 hours and 3 hours, according to Langendorff method. The results were as follows; 1. The norepinephrine content of isolated myocardium was decreased by the perfusion of guanethidine. 2. The norepinephrine content in the perfusate from isolated myocardium was increased by the perfusion of guanethidine. 3. Total norepinephrine content in both of isolated myocardium and the perfusate from isolated myocardium was increased progressively by the perfusion of increasing concentration of guanethidine in Tyrode solution and the prolongation of perfusion.

Morphine이 Norepinephrine 代謝에 미치는 影響 : Ⅰ. Morphine이 家兎의 腦, 心臟 및 腎臟의 norepinephrine 含量과 轉換에 미치는 效果 Ⅰ. The effect of morphine on the content and turnover of norepinephrine in the brain, heart and kidney of rabbits

千然淑 고려대학교 의과대학 1979 고려대 의대 잡지 Vol.16 No.2

Numerous studies using a variety of species implicated brain catecholamin in the analgesic activity of morphine. Yet the ability of single doses of morphine to change brain norepinephrine level would appear to be a species dependent phenomenon. This study was performed to observe the effect of morphine on the content and turnover of norepinephrine(NE) in the brain, heart and kidney of rabbits. The results were obtained as follows ; 1. Various doses (5, 10 and 20㎎/㎏) of morphine increased the brain NE and renal NE content and decreased the cardiac NE content 20 minutes after the morphine injection, and these NE changing effects of morphine were dose-dependent. 2. The time courses (20, 60 and 90 min.) of the effect of morphine (10 and 20㎎/㎏) on the NE content in several organs of rabbits showed an initial increase in the brain NE content, acute decrease in the cardiac NE content and gradual increase in the renal NE content. 3. The initial increase of brain NE content induced by morphine was continuously increased by iproniazid pretreatment, but the decreased cardiac NE and increased renal NE content induced by morphine were inhibited by the pretreatment.

Lithium과 Chlorpromazine의 腦組織中 Catecholamine 含量과 血漿 Corticosterone値에 미치는 影響

洪起男,千然淑 최신의학사 1984 最新醫學 Vol.27 No.3