인간$\cdot$환경$\cdot$건설을 하나로

천세윤 대한기계설비건설협회 2003 기계설비 Vol.155 No.-

제언 - '건설기술개발'이 곧 경쟁력이다

천세윤,Cheon, Se-Yun 대한기계설비건설협회 2013 기계설비 Vol.274 No.-

뮤지컬 전용공간의 건축적 계획에 관한 연구

천세윤(Chun.Se Yoon),최재희(Choy.Jae Hee) 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The musical, in all its various forms, is very much a "living art form."<br/> Musical Theater is not only the most popular form of modem art of the 20th century, but it evolved into a from that is both entertaining and intellectually stimulating; it successfully shifted from pure popular entertainment to recognition of<br/> the value of musical theatre as a higher art from.Currently in Korea, it is almost impossible to overestimate the role musical theatre plays in the economic life, and hence it is the most adequate for international business in the future.<br/> In order to successfully penetrate into the global business, Korean musical theater must mature into a higher from of art to uplift the cultural level of our country. However. the lack of space only for musicals is a critical problem that is<br/> holding back Korean musical theater from entering the main streamline and prospers in the industry of culture.<br/> Therefore, the main purpose of this research project is to find solutions to the fundamental problem stated previously, which is to provide actors with the space for developing their professionalism.In addition, musical theater must become more easily attainable from of popular entertainment that will ultimately appeal to all audiences.

Solanum tuberosum L. cv Jayoung Epidermis Extract Inhibits Mite Antigen-Induced Atopic Dermatitis in NC/Nga Mice by Regulating the Th1/Th2 Balance and Expression of Filaggrin

양갑식,천세윤,정경숙,이성진,홍철희,이경태,장대식,정진철,권오근,남정환,안효진 한국식품영양과학회 2015 Journal of medicinal food Vol.18 No.9

Solanum tuberosum L. cv Jayoung ( JY) is a potato with dark purple flesh and contains substantial amounts of polyphenols. In this study, we evaluated the therapeutic effects of S. tuberosum L. cv JY in a mouse model of Dermatophagoides farinae body (Dfb)-induced atopic dermatitis (AD). The ethanol extract of the peel of JY (EPJ) ameliorated Dfbinduced dermatitis severity, serum levels of immunoglobulin E (IgE) and thymus and activation-regulated chemokine. Histological analysis of the skin also revealed that EPJ treatment significantly decreased mast cell infiltration. The suppression of dermatitis by EPJ treatment was accompanied by a decrease in the skin levels of type 2 helper T-cell cytokines such as interleukin (IL)-4, IL-5, and IL-13. The induction of thymic stromal lymphopoietin, which leads to a systemic Th2 response, was also decreased in the skin by EPJ. Nuclear translocation of nuclear factor-κB p65 was decreased by EPJ in Dfb-induced NC/Nga mice. The protein expression of filaggrin in the AD-like skin lesions was restored by EPJ treatment. These results suggested that EPJ may be a potential therapeutic tool for the treatment of AD.

Rice Hull Extract Suppresses Benign Prostate Hyperplasia by Decreasing Inflammation and Regulating Cell Proliferation in Rats

김채윤,정경숙,천세윤,이종현,박윤범,안효진 한국식품영양과학회 2016 Journal of medicinal food Vol.19 No.8

Even though rice hull has various physiological functions with high antioxidant potential, the molecular mechanism(s) underlying the effects of rice hull on benign prostatic hyperplasia (BPH) have not been evaluated. The aim of this study was to determine the protective effect of rice hull water extract (RHE) against BPH, which is a common disorder in elderly men and involves inflammation that induces an imbalance between cell proliferation and cell death. In this study, RHE-treated mice exhibited lower prostate weights and ratios of prostate weight to body weight compared to those for the BPH-induced group. In addition, RHE-treated mice had lower serum levels of dihydrotestosterone, mRNA expression of 5areductase2, and protein expressions of proliferating cell nuclear antigen (PCNA). Furthermore, RHE treatment significantly decreased cell proliferation by regulating the expression levels of inflammatory-related proteins (iNOS and COX-2) and apoptosis-associated proteins (Fas, FADD, procaspase-8, -3, and Bcl-2 family proteins). These results suggest that RHE could protect against the development of BPH through its anti-inflammatory and apoptotic properties and has good potential as a treatment for BPH.

Anti-inflammatory Effects of Ethanol Extract of Various Korean Compositae Herbs in LPS-induced RAW 264.7 Macrophages

서민규,강윤미,정경숙,천세윤,박종혁,이영철,안효진 대한본초학회 2017 大韓本草學會誌 Vol.32 No.2

Objective : This study was designed to evaluate candidate materials as anti-inflammation agent from extracts of various Korean Compositae herbs in Hwaak mountain. Among Korea medicinal herbs, Ainsliaea acerifolia (AA) belongs to the Compositae family, has been used for the treatment of rheumatic arthritis. However, AA has not been previously reported to have an anti-inflammatory effect. Therefore, we investigated the anti-inflammatory effects of AA and its underlying molecular mechanisms in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages. Methods : Cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay in RAW 264.7 macrophages. Nitric oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by enzyme immunoassay (EIA) kits in LPS-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase, and cyclooxygenase-2 (COX-2) and p65 subunit of nuclear factor-κB (NF-κB) were determined by Western blot analysis. Results : Among 8 extracts of Korean Compositae herbs tested, AA showed the inhibition of NO production without cytotoxicity. Consistent with the observation, AA reduced the expression levels of iNOS and COX-2 proteins in LPS- simulated RAW 264.7 macrophages in dose-dependent manner. In addition, AA inhibited the productions of TNF-α and IL-6 in LPS-simulated RAW 264.7 macrophages. However, AA did not inhibit activation of p65 NF-κB in LPS- simulated RAW 264.7 macrophages. Conclusion : These results suggest that down-regulation of iNOS, COX-2 protein expression and TNF-α and IL-6 production by AA are responsible for its anti-inflammatory effects.

Anti-inflammatory Effects of Ethanol Extract of Korean Medicinal Plants at Hwaak Mountain in LPS-induced RAW 264.7 Macrophages

강윤미,전은진,정경숙,천세윤,박종혁,한유창,안효진 대한본초학회 2017 大韓本草學會誌 Vol.32 No.2

Objectives : This study was conducted to investigate candidate materials as anti-inflammatory agent from extracts of Korean medicinal plants in Hwaak mountain. Ligustrum obtusifolium (LO) is a Korea medicinal plants that commonly used for robustness and hemostasis. It has been reported that LO has exhibited anti-ischemic, anti-oxidative, anti- hypolipidemic, anti-tumor and hypoglycemic effects. However, LO has not been previously reported to have an anti- inflammatory effect. Therefore, we have evaluated the anti-inflammatory effects of LO and its underlying molecular mechanisms in LPS-induced RAW 264.7 macrophages. Methods : Cell viability was determined by MTT assay in RAW 264.7 macrophages. Nitric Oxide (NO) was measured with Griess reagent and pro-inflammatory cytokines were detected by ELISA in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. Protein expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and p65 subunit of nuclear factor-κB (NF-κB) were determined by Western blot analysis. Results : Among 15 extracts of Korean medicinal plants tested, Ligustrum obtusifolium (LO) showed the inhibition of NO production without cytotoxicity. LO reduced the expression levels of iNOS and COX-2 proteins in LPS-simulated RAW 264.7 macrophages in dose-dependent manner. Consistent with these data, LO inhibited the productions of TNF-α, IL-6, and IL-1β in LPS-simulated RAW 264.7 macrophages. Furthermore, LO attenuated the LPS-induced nuclear translocation of p65 NF-κB in RAW 264.7 macrophages involving suppression of NF-κB activation. Conclusions : Taken together, these results suggest that the anti-inflammatory effects of LO is associated with regulation of inflammatory mediators via inhibition of NF-κB activation in LPS-treated RAW 264.7 macrophages.

LPS로 자극한 RAW 264.7 세포에서 중국 연변에 자생하는 약용 식물 에탄올 추출물의 항염증 효과 연구

박예진 ( Yea-jin Park ),서종환 ( Jong-hwan Seo ),길태영 ( Tae-young Gil ),천세윤 ( Se-yun Cheon ),박인철 ( Ren-zhe Piao ),이상우 ( Sang-woo Lee ),차윤엽 ( Yun-yeop Cha ),안효진 ( Hyo-jin An ) 대한본초학회 2018 大韓本草學會誌 Vol.33 No.6

Objectives : This study was fulfilled to investigate nominee materials as anti-inflammatory agent from ethanol extract of Chinese medicinal plants in Yanjin. Among the 20 candidates, we selected most effective one, the ethanol extract of Cicuta virosa L. (CVL). The mechanism underlying the anti-inflammatory effects of CVL is not clearly identified as yet. Accordingly, we clarified the anti-inflammatory effects of CVL and its underlying molecular mechanisms in LPS-stimulated RAW 264.7 macrophages. Methods : RAW264.7 macrophages were incubated with CVL (12.5, 25, or 50 μM) and/or lipopolysaccharide (LPS) (1 ㎍/㎖). Cytotoxicity was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide assay and the level of nitric oxide (NO) production was measured with Griess reagent. The prostaglandin E2 (PGE2) production was measured with enzyme immunoassay kits and the protein expression of inducible nitric oxide synthase (iNOS) was determined using Western blot analysis. Results : Among the 20 ethanol extract of Chinese medicinal plants of Yanjin tested, CVL significantly reduced the production of NO in a dose-dependent manner via inhibition the protein expressions of iNOS without cytotoxicity on the LPS-stimulated RAW 264.7 macrophages. In addition, CVL also effectively declined the production of PGE2 in LPS-simulated RAW 264.7 macrophages. Conclusions : Taken together, these data presented in this study demonstrate that CVL possesses anti- inflammatory activity by suppressing the production of pro-inflammatory mediators NO and PGE₂, and pro- inflammatory protein iNOS expression in LPS-stimulated RAW 264.7 macrophages.