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귀리 원형질체의 칼슘이온 변화에 미치는 Chitosan 의 영향
이종화,채쾌 ( Chong Hwa Lee,Quae Chae ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.5
Concentration changes of the cytoplasmic free Ca^(2+) in oat cell were investigated after interaction between chitosan and cell. Calcium ion specific fluorescence probe (Quin-2 AM) was loaded into the cells and the concentration of cytoplasmic free Ca^(2+) was determined from the fluorescence intensity of Ca^(2+)-Quin 2 complex. In the resting state, [Ca^(2+)]_i of oat cells (1×10^6 ml) was determined to be 40 nM. At low concentration of chitosan ($lt;6 ㎎/㎖), [Ca^(2+)]_i was decreased, but it began to increase at high concentration of chitosan decreased, but it began to increase at high concentration of chitosan (11.0∼26 ㎎/㎖) even in the presence of excess EGTA in the medium. However, Ca^(2+)-transport by the chitosan was completely inhibited by addition of Ca^(2+) channel blocker (120μM verapamil) and then Ca^(2+) influx was recovered by addition of Ca^(2+) ionophore (10 μM A 23187). In the presence of 1 mM Ca^(2+) in the medium, the mode of Ca^(2+) transport caused by the interaction between chitosan and cell was appeared as three different phases by variation of incubation time; 1) rapid increase of [Ca^(2+)]_i (=T₁; 2 sec), 2) decrease of [Ca^(2+)]_i (= T₂ phase; 2∼20 sec). Optimal concentration of chitosan for the initiation of its response was about 6 ㎎/㎖.
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Fusarium solani 포자에 의한 인삼 phytoalexin 의 생합성 및 유도에 관한 연구
이종화,박화진,채쾌 ( Chong Hwa Lee,Hwa Jin Park,Quae Chae ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.1
In order to find out the elicitation of the phytoalexin in ginseng root tissues, the spore of Fusarium solani was used as an elicitor. The macroscopic symptoms on the sliced root tissues and the measurement of the elicitor activity suggested us that the spore of Fusarium solani elicited the phytoalexin, which was not identified yet, from the ginseng root. The UV spectral data of water and methanol extracts after treatment with the fungal spore were compared with the ones reported in other plants, and it seemed that the phytoalexin formed in ginseng root might be the phenolic compounds. It was observed that phytoalexin started to accumulate from 15 h after infection of elicitors.
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